38 results about "Miniature swine" patented technology

The invention provides a porcine reproductive and respiratory syndrome virus receptor CD163 knock-out swine and a cultivation method thereof. A constructed CD163 gene knock-out carrier pSSC-Larm-Sarm-CD163 uses a genome of Chinese experimental miniature swines as a template, and adopts a PCR method to amplify a homologous left arm and a homologous right arm of the swine CD163 gene; the cloned homologous left arm and the homologous right arm of the CD163 gene are subcloned to a pSSC-9 carrier respectively; and the porcine reproductive and respiratory syndrome virus receptor CD163 knock-out swine is cultivated, wherein the CD163 gene is not expressed, and the swine is not infected by the porcine reproductive and respiratory syndrome virus.

The invention relates to an experimental miniature pig feeding fence, and belongs to the technical field of experimental miniature pig breeding. The experimental miniature pig feeding fence comprises a supporting frame, a feces leaking plate, a feces tray and barriers, wherein the bottom of the supporting frame is connected to the feces leaking plate in a clamped mode, feces leaking holes are evenly formed in the feces leaking plate at intervals, the feces tray located below the feces leaking plate is mounted on the supporting frame in a sliding mode, the fence is fixedly mounted on the periphery of the supporting frame, vertical sliding rods are correspondingly mounted in the middle of the front side and the middle of the rear side of the supporting frame, and sliding grooves matched with the sliding rods are formed in the front ends and the rear ends of the barriers correspondingly. According to the experimental miniature pig feeding fence, the barriers convenient to disassemble and assemble are arranged in the middle of the feeding fence, the barriers are disassembled or assembled according to the feeding scale, the pig feeding space is reduced or enlarged in applicability, the feeding space can be saved to the maximum extent, the feeding cost can be reduced, meanwhile, feces of pigs in the feeding fence can be rapidly discharged by arranging the feces leakage plate and the feces disc, the clean feeding environment is guaranteed, the miniature pigs can grow healthily, and breeding, popularization and utilization of clean-level and SPF-level experimental miniature pigs are accelerated.

The invention discloses a specific microsatellite primer composition for identifying experimental minipig varieties and a method for identifying experimental minipig varieties. The primer composition includes 3 pairs of primers. The present invention is based on the microsatellite sequence in the genome of the miniature pig breed, and obtains the specific microsatellite sequence and primers among different breeds of miniature pigs for experiments through population test verification and screening, optimizes the test conditions and usage methods of the primers, and solves the problem of Identification of miniature pig breeds for experimental use.

The invention relates to the biological cell technology field, particularly a method for separating and culturing miniature swine bone marrow-derived endothelial progenitor cells. The conventional EPCs culture method has the disadvantages of complexity and greatly reduced cell harvest rate by using immunomagnetic bead separation. The method in the invention comprises the steps of directly using myelomonocyte to conduct culture in vitro to obtain EPCs, adding growth factors in culture solution, planting in a definite density in a culture flask / vessel or a glass sheet enveloped by fibronectin,and conducing appropriate culture to obtain EPC. The method with high repeatability simplifies the steps of the conventional culture method, effectively avoids unnecessary cell loss caused by the conventional separation and culture method, increases the pick-up rate of EPCs, and saves a large amount of funds. The method of the invention also prepares for clinical application in future, and can reduce the usage of bone marrow and provide a technology platform for curing traumatic or ischemic diseases by autotransfusion.

The invention relates to an underwater breathing mask for Bama miniature pigs. The underwater breathing mask comprises a silica gel sleeve (1), a hard shell (2), a first circular supporting plate (3),a second circular supporting plate (7), a plurality of ventilation copper pipes (4) and a plurality of silica gel pads (6); the silica gel sleeve (1) is of a straight cylindrical structure with an opening in one end, and the opening is in a horn shape; the silica gel sleeve (1) is divided into a first rubber sleeve section (8) and a second rubber sleeve section (9); the first rubber sleeve section (8) is nested in the hard shell (2), and a first circular supporting plate (3) and a second circular supporting plate (7) are correspondingly arranged on the outer side and the inner side of the first rubber sleeve section (8) respectively; wherein the hard shell (2) is of a straight cylindrical structure with an opening at one end; and the two ventilation copper pipes (4) are respectively and correspondingly inserted inwards from the round holes on the hard shell (2), and the silica gel pads (6) are arranged between the two ventilation copper pipes (4).

The present invention discloses an experimental method for evaluating eye irritation by using miniature pig erythrocytes. The method mainly comprises: (1) preparing a suspension of miniature pig erythrocytes; (2) carrying out a miniature pig erythrocyte hemolysis test; (3) carrying out a protein denaturation experiment; (4) predicting a model; (5) carrying out classification and judgment. According to the present invention, the source of the erythrocytes is reliable and safe; the tested material is easy to obtain, and has good uniformity; the test system is simple and cheap, and no special equipment is required; the detection method is rapid, sensitive and universal; the method of the present invention can be directly applicable for safety evaluations of chemicals, cosmetics, pesticides, eye medicines and other substances possibly contacting the eyes, wherein the chemicals comprise surfactants, disinfection products, detergent and the like, the cosmetics comprise shampoo, bath foam, eye cream and the like; the method further can be applicable for rapidly screening, classifying and identifying the eye irritations of the raw materials, the formulas or the products.

The invention discloses a method for preparing a human complement regulatory protein miniature pig at the Rosa26 site. The invention inserts the human complement regulatory protein gene into the pig ROSA26 gene site through site-directed gene recombination, thereby constructing The minipigs constitutively expressing human complement regulatory proteins were obtained. The constructed minipigs not only avoided the unclear genetic background caused by the random insertion of foreign sources and the unstable expression level caused by the segregation of chromosomes during the breeding process of offspring, but also laid a solid foundation for future development. The study of xenotransplantation and immune-related diseases provides important animal resources and has important clinical application value.

The invention relates to an underwater breathing support system for Bama miniature pigs, comprising: a high-pressure gas cylinder (13), a secondary decompression device (14) and a breathing mask (15); a high-pressure gas cylinder (13), The secondary decompression device (14) and the breathing mask (15) are connected to each other through ventilation connectors; the present invention can be reused, and can achieve 100% sealing by cooperating with adhesive tape and glass glue, which fully guarantees that the Bama miniature pigs can survive in Underwater breathing, will not drowning, choking, and avoid subsequent test failures.

The invention relates to an underwater breathing mask for Bama miniature pigs. The underwater breathing mask comprises a silica gel sleeve (1), a hard shell (2), a first circular supporting plate (3),a second circular supporting plate (7), a plurality of ventilation copper pipes (4) and a plurality of silica gel pads (6); the silica gel sleeve (1) is of a straight cylindrical structure with an opening in one end, and the opening is in a horn shape; the silica gel sleeve (1) is divided into a first rubber sleeve section (8) and a second rubber sleeve section (9); the first rubber sleeve section (8) is nested in the hard shell (2), and a first circular supporting plate (3) and a second circular supporting plate (7) are correspondingly arranged on the outer side and the inner side of the first rubber sleeve section (8) respectively; wherein the hard shell (2) is of a straight cylindrical structure with an opening at one end; and the two ventilation copper pipes (4) are respectively and correspondingly inserted inwards from the round holes on the hard shell (2), and the silica gel pads (6) are arranged between the two ventilation copper pipes (4).