20 results about "Miniature swine" patented technology

The invention relates to the technical field of biological gene detection, and particularly discloses a miniature pig SNP (Single Nucleotide Polymorphism) molecular marker combination and application thereof. The miniature pig SNP molecular marker combination disclosed by the invention comprises at least one of 221 SNP molecular markers, the physical positions of the 221 SNP molecular markers are determined by performing sequence alignment on whole genome sequencing with the coverage rate of 10 * on the basis of miniature pigs, and the site information of the 221 SNP molecular markers is shown in a table 1. The miniature pig SNP molecular marker combination provided by the invention is used for detecting the SNP genotyping of miniature pigs, and has the properties of novelty, stability, economy and high efficiency.

The invention belongs to the technical field of medicines, and particularly relates to application of harpagide in preparation of a medicine for treating constipation. The technical problem to be solved by the invention is to provide a safer, milder and new effective choice for treating and relieving constipation. The technical scheme for solving the technical problem is to provide application of harpagide in preparation of drugs or health care products for treating or relieving constipation. The harpagide achieves or even exceeds the treatment effect of the fluid-increasing decoction compound. Meanwhile, in the small pigs, the constipation symptom of the small pigs in the gestation period is also obviously relieved. When the harpagide is used as a medicine for treating constipation, a safer, more efficient and standardized treatment effect can be achieved, a safer and milder treatment choice is provided for patients with constipation, and the harpagide has a very good application prospect.

The invention belongs to the field of animal experiment devices, and discloses a fixing device for index collection of miniature pigs for experiments. Comprising a support which is formed by supporting a square frame through four supporting legs. The fixing bag is installed on the two opposite beams of the square frame and naturally droops to form an arc shape, the fixing bag is provided with four through holes matched with the positions of the four limbs of the miniature pig, and the fixing bag is provided with a rectangular hole; one end of the cylinder is installed on the through hole, the other end of the cylinder is closed, an operation hole is formed in the outer side of the cylinder, and elastic pads are arranged at the bottom and the inner side of the cylinder; the bandage is arranged on the square frame; a breathing belt can be smoothly wound through the rectangular hole, operation on the fixing device can be achieved, the risk that a miniature pig is fixed with bare hands to escape is eradicated, and the risk that collection equipment is damaged when the miniature pig struggles is also eradicated.

This application relates to the technical field of biological gene detection, specifically disclosing a miniature pig SNP molecular marker combinatorial system and its application. The miniature pig SNP molecular marker combinatorial system disclosed in this application includes at least one of 221 SNP molecular markers. The physical locations of these 221 SNP molecular markers are determined based on sequence alignment during 10× coverage whole-genome sequencing of miniature pigs. The site information of the 221 SNP molecular markers is shown in Table 1. The miniature pig SNP molecular marker combinatorial system provided in this application is used for detecting SNP genotyping in miniature pigs and possesses novel, stable, cost-effective, and efficient properties.

The invention discloses application of water microspheres of modified polysaccharide in promoting wound skin regeneration and inhibiting wound skin fibrosis. In the invention, compared with a Control group (silicone oil) as a control and an unmodified polysaccharide water microsphere group (AVBEC), when the modified polysaccharide water microsphere group (AVBER) is externally applied to the skin of a bama miniature pig in a deep II-level scald model, the wound healing can be effectively promoted, the inflammation degree and duration can be controlled, and the extracellular matrix composition and plasticity can be regulated; expression of fibrosis marker protein is reduced, and the dual effects of promoting skin regeneration and inhibiting fibrosis are achieved. The problems that natural extracts and common polysaccharide microsphere products are low in biological activity and poor in stability are solved, and new functions of modified polysaccharide are found.

The invention discloses a preparation method of a Guizhou miniature pig piglet damp-heat diarrhea model. The method comprises the steps that Guizhou miniature pigs are placed under the conditions of poor ventilation and temperature and humidity to be fed with feed, 1 * 10 < 9 > CFU / mL / kg / d of escherichia coli is fed every day at the same time, and continuous modeling is conducted for 3-5 days; the feed comprises the following components in percentage by weight: 37.8%-79.7% of corn, 15%-20% of crude protein, 1%-7% of crude fiber, 1%-9% of crude ash, 0.5%-1.2% of calcium, 1.5%-10% of lysine, 0.5%-1.5% of total phosphorus, 0.3%-0.8% of sodium chloride and 1%-13% of water. The Guizhou miniature pig piglet damp-heat diarrhea model prepared by the method is good in stability and repeatability, standard in evaluation and suitable for clinical practice.

An experimental miniature pig rearing cage includes a rearing cage, a support base, and a heater. The rearing cage has a first switch door on its outer side and a second switch door on one side. The lower part of the rearing cage is connected to the support base, and the upper part of the support base has a fixed base. The lower part of the rearing cage has a lying board, and the lower part of the lying board is connected to the fixed base. The lying board has a heating chamber inside, and a heating pipe is connected to the inner side of the heating chamber. The heating pipe passes through the lying board and connects to the heater. The bottom of the heater is connected to the support base. The lower part of the rearing cage has multiple drainage troughs. Before use, the cage is disinfected, improving the survival rate of miniature pigs. The temperature of the miniature pigs' living environment can be adjusted according to needs. The cage is simple to operate, has a reasonable structure, and can achieve centralized collection of feces, reducing the probability of disease in miniature pigs and facilitating their rearing.

The application discloses a method for establishing a Bama miniature pig spleen deficiency and dampness excess model, and belongs to the technical field of animal models. The method is to reduce the immunity of the body by intraperitoneal injection of cyclophosphamide, and then to stimulate the Bama miniature pig by using high-sugar and high-fat feed and senna leaves in combination with a high-humidity external environment. After 8 weeks, the Bama miniature pig has the clinical manifestations of the spleen deficiency and dampness excess, such as body fatness, fatigue and laziness, white and slippery tongue fur, and loose and watery stool, and the model is verified in the reverse direction by using the classic compound Shenglin Bai Zhusan. The Bama miniature pig spleen deficiency and dampness excess model is applied to the research of human spleen deficiency and dampness excess and the development of spleen-invigorating and dampness-expelling drugs. The model provides a new model for standardizing, standardizing and scientifically evaluating the research of spleen-invigorating and dampness-expelling traditional Chinese medicines, and has the advantages of good practicability, strong conformity, simplicity and objectivity, and conforms to the guidance of the theory of traditional Chinese medicine syndromes, and has a wide application prospect.

The invention provides a construction and evaluation method of a porcine retinitis pigmentosa disease model, and belongs to the technical field of animal models of human diseases. The invention provides a method for constructing and evaluating a porcine retinitis pigmentosa disease model, which comprises the following steps: applying iodoacetic acid to the vein of a miniature pig to construct the porcine retinitis pigmentosa disease model; after the iodoacetic acid is applied to the veins of the miniature pig, the miniature pig is subjected to ophthalmic examination; the ophthalmic examination comprises eye morphological structure and function examination. According to the construction method, iodoacetic acid is used as a modeling reagent, directional damage to the miniature pig retina photoreceptor cells can be rapidly achieved within one week, and the modeling rate is high. Moreover, after the iodoacetic acid is applied to the veins of the miniature pig, the miniature pig is subjected to ophthalmic examination including eye morphological structure and function examination, analysis is comprehensive, and the constructed model is stable, reliable, good in uniformity, long in maintenance time and easy to copy.

The invention belongs to the technical field of bama miniature pig experiments, and particularly relates to a fixator for constructing a bama miniature pig DCUs model, which comprises an outer sleeve and an inner sleeve arranged in the outer sleeve, first openings are formed in the left side and the right side of the bottom end of the outer sleeve, and second openings are formed in the left side and the right side of the bottom end of the inner sleeve. A supporting strip is fixedly connected into the inner sleeve, a twisting strip is fixedly connected to the upper end of the screw shaft, a screw hole is formed in the middle of the supporting strip, the screw shaft is connected into the screw hole in a screwed mode, a connecting base is arranged at the bottom end of the screw shaft, and connecting rods are arranged on the left side and the right side of the bottom end of the connecting base. Compared with an existing fixator which adopts a sewing fixing mode, the fixing device has the advantages that the operation is more convenient and faster, skin inflammation and ulceration on the surface of a wound are not easily caused, and the use is better.

The invention provides ultralow-temperature cryopreservation liquid for white inbred line miniature pig sperms and application of the ultralow-temperature cryopreservation liquid, and belongs to the technical field of pig sperm cryopreservation. The problems that an existing commercial pig sperm cryopreservation technology system and an inbred line white miniature pig cryopreservation technology system are not matched, the sperm quality is reduced and the like are solved. The cryopreservation solution comprises the cryopreservation solution for the sperms of the inbred line white miniature pigs, and the cryopreservation solution is prepared from the following components: a conventional cryopreservation solution, betaine and resveratrol. The method is mainly used for cryopreservation of inbred white miniature pigs.

The present invention relates to a method for preparing a melatonin-producing miniature pig cell line, and a use thereof. It has been confirmed that a miniature pig-derived pineal gland cell line of the present invention may be used as a pineal gland cell line by having a single-characteristic cell line isolated. In addition, it has been confirmed that, in the isolated pineal gland cell line, the pineal gland markers and melatonin synthesis proteins AANAT, ASMT, SAG, ADRB1, and SLC15A1 are expressed, and it has been confirmed that serotonin and melatonin secreted by pinealocytes are extracellularly secreted. In addition, it has been confirmed that the pineal gland cell line expresses pineal gland markers and proteins. In addition, it has been confirmed that, in a cell line in which the pineal gland cell line has MTNR1A marked with red fluorescence, fluorescence expression increases in a melatonin-dependent manner, and the fluorescence expression and MTNR1A are reduced due to a melatonin antagonist, and thus it has been confirmed that the present invention may be used for brain disease or brain cancer screening by using same.

This invention discloses the application of the AKR1C1 gene and its encoded protein in breeding pig breeds with superior metabolic function. It belongs to the field of genetic engineering technology, and particularly relates to the application of the AKR1C1 gene in breeding pig breeds with superior metabolic function. The nucleotide sequence of the AKR1C1 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the AKR1C1 gene is shown in SEQ ID NO.2. This invention provides the AKR1C1 gene of the Guangxi Bama miniature pig, clarifies the nucleotide sequence of the AKR1C1 gene and the amino acid sequence of the encoded protein, and provides the application of the AKR1C1 gene in regulating bile acid metabolism in pig liver, providing a new molecular target for pig genetic breeding and contributing to the breeding of pig breeds with superior metabolic function.

The present invention relates to a method for preparing a melatonin-producing miniature pig cell line, and a use thereof. It has been confirmed that a miniature pig-derived pineal gland cell line of the present invention may be used as a pineal gland cell line by having a single-characteristic cell line isolated. In addition, it has been confirmed that, in the isolated pineal gland cell line, the pineal gland markers and melatonin synthesis proteins AANAT, ASMT, SAG, ADRB1, and SLC15A1 are expressed, and it has been confirmed that serotonin and melatonin secreted by pinealocytes are extracellularly secreted. In addition, it has been confirmed that the pineal gland cell line expresses pineal gland markers and proteins. In addition, it has been confirmed that, in a cell line in which the pineal gland cell line has MTNR1A marked with red fluorescence, fluorescence expression increases in a melatonin-dependent manner, and the fluorescence expression and MTNR1A are reduced due to a melatonin antagonist, and thus it has been confirmed that the present invention may be used for brain disease or brain cancer screening by using same.

The invention provides a Guangxi Bama miniature pig fetus kidney fibroblast subculture method, and belongs to the technical field of cell subculture, and the method comprises the following steps: step 1, obtaining a cell material, step 2, carrying out cell subculture, step 3, carrying out cryopreservation and recovery, step 4, drawing a cell growth curve, and step 5, identifying cell types. According to the method, a mechanical method and an enzyme digestion method are combined, primary kidney fibroblasts of Guangxi Bama miniature pig fetuses are separated and cultured, and a primary cell line capable of being subjected to conventional subculture, freezing and resuscitation is obtained; the proliferation potential is detected through a CCK8 method, cell identification is carried out through immunofluorescence, and the result shows that newborn Guangxi Bama miniature pig kidney fibroblasts are successfully separated and can be subjected to mass passage and freezing in vitro, and the newborn Guangxi Bama miniature pig kidney fibroblasts are already subjected to passage to 20 generations or above at present and grow well; after freezing recovery, normal culture and passage can still be realized.

The application discloses a method and application of targeting knockout of a pig TXLNB gene locus, and belongs to the technical field of gene editing engineering. The application designs a TXLNB gene knockout method based on a CRISPR / Cas9 system. Specifically, by screening gRNA target points and gRNA combinations, it is found that when the two ends of a targeted TXLNB exon 3 are adopted, a specific gRNA combination can realize efficient TXLNB gene deletion. After the related gene fragments in pig fibroblasts are deleted by the method, the animal model formed by transplanting the knockout cells into a small pig in vivo has obvious phenotypes, and has great potential in pig breeding related research.

The invention discloses a method for targeted knockout of a pig TXLNB gene locus and application, and belongs to the technical field of gene editing engineering. The invention designs a TXLNB gene knockout method based on a CRISPR / Cas9 system, and particularly, by screening a gRNA target and a gRNA combination, it is found that efficient TXLNB gene deletion can be realized by adopting a specific gRNA combination when two ends of a TXLNB exon 3 are targeted. The animal model formed by deleting related gene segments in the porcine fibroblasts and then transplanting the knocked-out cells into miniature pig bodies has obvious phenotypes and has huge potential in related research of pig breeding.

The invention relates to the technical field of medical evaluation and detection, in particular to a Qi deficiency and blood stasis syndrome large animal model making method which comprises the following steps: step 1, feed proportioning and feeding: miniature pigs are continuously fed with high-calorie feed for 6 months, and the high-calorie feed comprises the following components in percentage by weight: 10% of beef tallow, 6% of peanut oil, 0.5% of cholate, 3% of cholesterol and the balance of water; the acesulfame potassium accounts for 2.5%, and the basal feed accounts for 78%. According to the preparation method of the qi deficiency and blood stasis syndrome model, animals are subjected to long-term chronic high-fat diet, stomach impairment and spleen impairment according to the traditional Chinese medicine theories of'fat, sweet and thick taste impairment on spleen and qi 'and'qi consumption due to fatigue', so that the source of vital energy can be reduced, and the blood stasis syndrome caused by phlegm-dampness can be helped to occur; exhaustive swimming is used for increasing the consumption of healthy qi, qi is the commander of blood, qi deficiency causes inability to promote blood circulation, and blood in meridians is stagnated and deposited to form blood stasis.

The invention relates to the technical field of biomedical experimental animal models, in particular to a drug induction-based miniature pig diabetes mellitus model construction method and application. The drug-induced miniature pig diabetes model construction method comprises the following steps: mixing a citric acid solution with a sodium citrate solution to obtain a mixed solution, and adjusting the pH value of the mixed solution to 4.2-5.0 to obtain a citric acid buffer solution; under the dark condition, streptozotocin is dissolved in the citric acid buffer solution, and a streptozotocin solution is obtained; the prepared streptozotocin solution is injected into the ear edge vein of the fasting miniature pig at a time, and construction of the miniature pig diabetes mellitus model is completed; the injection dosage in the step S3 is 140 to 160 mg / kg. The construction steps of the miniature pig diabetes model are simplified, the long-term stable miniature pig diabetes model can be constructed, and a simpler, more convenient and more stable model construction method is provided for research on a miniature pig diabetes disease mechanism and a treatment method.

The present invention relates to a porcine circovirus infection-preventing vaccine composition in which the amount of an antigen is adjusted. The vaccine composition according to the present invention has been verified to be safe, and, unlike existing patents, has also been identified to effectively induce protective immunity against PCV2d challenge vaccination in pigs (SPF minipigs) which are target animals. In addition, it has been identified that animals inoculated with the vaccine composition of the present invention have significantly reduced PCV2-related lesions. As such, effective protection against currently prevalent porcine circovirus is indicated, and thus the present invention can be widely utilized in the fields of porcine circovirus infection prevention and pig farming.