Autologous stem cells for treating renal insufficiency and preparation method thereof

A stem cell and kidney function technology, applied in animal cells, vertebrate cells, artificial cell constructs, etc., to achieve good safety, easy access, and no adverse reactions

Inactive Publication Date: 2011-08-10
SHANGHAI BIOMED UNION BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no literature report on the application of induced different...

Method used

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Embodiment Construction

[0018] Below in conjunction with specific embodiment the present invention is described in further detail:

[0019] a. Aseptically suck adipose tissue from the patient's abdomen or buttocks, wash it repeatedly with PBS buffer three times to remove red blood cells and other cell debris, cut it into pieces, and add 2 to 3 times the volume of 0.075% to 0.1% I Collagenase-type PBS buffer solution, fully shaken and digested in a 37°C water bath for more than 30min.

[0020] b. Then add an equal amount of DMEM culture medium containing 10% autologous serum or human AB serum to neutralize collagenase, centrifuge at 1000rpm for 10min, remove floating adipocytes and supernatant, add 140~180mM NH to the obtained cell pellet 4 Cl was resuspended in PBS buffer, and after standing at room temperature for 10 minutes to lyse the red blood cells, centrifuged at 1000 rpm for 10 minutes to discard the supernatant and obtain cell pellets.

[0021] c. After the obtained cell pellet was washed wi...

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Abstract

The invention provides autologous stem cells for treating renal insufficiency and a preparation method thereof. In the preparation method, autologous progenitor cells for treating renal insufficiency are generated by induced differentiation of adipose-derived stem cells which are obtained by separation from the adipose tissue of a patient, purification and amplification culture. The resource of the autologous progenitor cells is rich, the autologous progenitor cells can be obtained conveniently, and the preparation method is simple, convenient, easy, economic and practical. The prepared stem cells are subjected to autotransfusion to treat renal insufficiency with obvious treatment effect, high safety, no immunological rejection, no obvious toxic or side effects, no adverse reaction and less pain in patients. The invention provides a brand-new treatment means for patients with renal insufficiency and has a profound active significance.

Description

technical field [0001] The invention relates to a stem cell and a preparation method thereof, in particular to an adipose-derived autologous stem cell for treating renal insufficiency and a preparation method thereof. Background technique [0002] Renal insufficiency (renal insufficiency) is caused by a variety of reasons. The glomerulus is severely damaged, which makes the body excrete metabolic waste and regulate water, electrolyte, acid-base balance and other aspects of clinical syndrome. Divided into acute renal insufficiency and chronic renal insufficiency. Renal insufficiency can be divided into four stages: the first stage of renal function reserve compensation; the second stage of renal insufficiency; the third stage of renal failure; the fourth stage of uremia or the end stage of renal insufficiency. It is a globally recognized intractable disease with a serious prognosis and is one of the main life-threatening diseases. Uremia, known as the second cancer, is the ...

Claims

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Application Information

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IPC IPC(8): C12N5/071
Inventor 许凤华吴国祥张绪敏
Owner SHANGHAI BIOMED UNION BIOTECHNOLOGY CO LTD
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