The invention discloses a safety protection enhanced organ low-temperature
machine perfusion preservation device and a method. The device comprises an organ box for holding an organ to be transplanted, a low-temperature
incubator, a
perfusion power source, a
perfusion loop, a perfusion preparation loop, a flushing loop, and sensor assemblies arranged on various pipeline loops. The perfusion loop is composed of the perfusion power source, a filter, a buffer, a bubble trap, a first perfusion pipeline, a
main channel pipeline and a second
branch channel pipeline of a Y-shaped pipeline, an organ arteriovenous
blood vessel and a second perfusion pipeline. The perfusion preparation loop is composed of the perfusion power source, a filter, a buffer, a bubble trap, a first perfusion pipeline, and a
main channel pipeline and a first
branch channel pipeline of a Y-shaped pipeline. The flushing loop is a flushing pipeline. According to the safety protection enhanced organ low-temperature
machine perfusion preservation device, the design of the perfusion loop is optimized, the bubble trap and the
bubble detector are added in the perfusion loop, and the perfusion loop and the flushing loop are used for selectively removing perfusion bubbles in real time, and the safety of perfusion is improved.