Safety protection enhanced organ low-temperature machine perfusion preservation device and method

Abstract

The invention discloses a safety protection enhanced organ low-temperature machine perfusion preservation device and a method. The device comprises an organ box for holding an organ to be transplanted, a low-temperature incubator, a perfusion power source, a perfusion loop, a perfusion preparation loop, a flushing loop, and sensor assemblies arranged on various pipeline loops. The perfusion loop is composed of the perfusion power source, a filter, a buffer, a bubble trap, a first perfusion pipeline, a main channel pipeline and a second branch channel pipeline of a Y-shaped pipeline, an organ arteriovenous blood vessel and a second perfusion pipeline. The perfusion preparation loop is composed of the perfusion power source, a filter, a buffer, a bubble trap, a first perfusion pipeline, and a main channel pipeline and a first branch channel pipeline of a Y-shaped pipeline. The flushing loop is a flushing pipeline. According to the safety protection enhanced organ low-temperature machine perfusion preservation device, the design of the perfusion loop is optimized, the bubble trap and the bubble detector are added in the perfusion loop, and the perfusion loop and the flushing loop are used for selectively removing perfusion bubbles in real time, and the safety of perfusion is improved.

Description

technical field [0001] The invention belongs to the field of biomedical instruments, and in particular relates to a low-temperature machine perfusion preservation device and method for enhanced in vitro safety protection of organs to be transplanted. Background technique [0002] Organ transplantation has become one of the effective measures to save the lives of patients with multiple organ failure. The emergence of organ cryopreservation technology provides a solution to solve the shortage of transplanted organ donors and maintain organ function in the process of ex vivo, which greatly improves the clinical application of organ transplantation technology. [0003] Pure cryopreservation and mechanical continuous perfusion are the most important methods for in vitro preservation of isolated organs in the past three decades. The simple cold storage method is characterized by reducing the metabolic rate of organs by providing a low temperature environment of 0-4 °C and usi...

AI Technical Summary

Problems solved by technology

[0004] However, the effective time limit for organ preservation using these methods is short, and it is difficult to preserve the isolated kidney for more than 24-30 hours in clinical practice.

Among them, the safety protection of the perfusion device itself, the timely treatment of possible failures, the physiological simulation of the organ preservation environment and other factors may affect the effect of organ preservation and the effective time limit of preservation.

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