The invention discloses a biological herbicide and a preparation method thereof, belonging to the technical field of using microorganisms to control farmland weeds. The herbicide takes Curvularia Boedijn (Curvularia Boedijn) Mds0404 strain CGMCCNO.1827 as the bacterial species, and the weight percentage content of its components and each component is: 80-95% of pure spores, 0-18% of chemical herbicides, and auxiliary agents 2-5%; prepared by the preparation of the culture medium, the activation of the strain Mds0404, the cultivation of the seed solution, the production, cultivation and collection of the spores of the strain, and the mixing of various components. The agent mainly controls crabgrass, and also kills foxtail, barnyardgrass, goosegrass and other weeds. It is safe for crops, especially for monocot crops, has wide application adaptability, low cost, and no pollution. It can be popularized and applied in crabgrass crabgrass and other weed-infested areas.