The invention discloses a method for rapidly detecting sulfonamides in meat by an improved
QuEChERS combined
enzyme inhibition colorimetric method, which uses a natural eutectic
solvent instead of a toxic
organic solvent as an extraction agent, reduces the harm to the environment and
human body, improves the extraction efficiency, and is beneficial to protecting the
enzyme activity, so that the detection is more sensitive and stable; the magnetic COF (TpPa-Cl) coated Fe3O4 synthesized by adopting a simple and rapid stirring method not only has good
chemical stability and can be repeatedly used, but also has excellent adsorption capacity and strong
magnetism, and when the magnetic COF (TpPa-Cl) coated Fe3O4 is used as an adsorbent for improving
QuEChERS, interfering substances in a sample can be effectively removed in a small amount, the pretreatment time is shortened, and the purification process is simplified; in the
enzyme inhibition colorimetric detection process, a
digital image colorimetric method of a
smart phone is combined for quantitative analysis. The whole method has the remarkable advantages of greenness, high efficiency, short time consumption, low cost, simplicity in operation, high sensitivity and the like.