7 results about "Italian bee" patented technology

The invention discloses application of jute nanocellulose or derivatives thereof in preparation of drugs or feeds for preventing and treating bee virus infection, and belongs to the technical field of bee breeding. It is found that the jute nanocellulose can significantly improve the survival rate of Italian bees infected with chronic paralysis virus (CBPV) and reduce the death rate of the Italian bees. The jute nano-cellulose can inhibit proliferation of CBPV in bees and reduce the copy number of viruses. Meanwhile, the level of intestinal probiotics reduced due to CBPV infection can be recovered, and the congenital immune defense capability of bees is improved. The jute nanocellulose can be used for preparing drugs or feed additives for preventing and treating CBPV infection, has important significance on preventing and treating chronic paralysis viruses of Italian bees, and has good market application value.

The present invention relates to the technical field of bee breeding, and particularly relates to a method for mixed feeding of Italian bees and Chinese bees. The method for mixed feeding of Italian bees and Chinese bees comprises the following steps: S1: Raise the Italian bee colony at one side position of a double-queen colony beehive; S2: After 2 to 3 days, transfer the Chinese bees into the beehive and raise them at the other side position, and an adjustable queen excluder is installed in the middle of the beehive to separate the two bee colonies; S3: Open both nest entrances, and both bee colonies can freely enter and exit their respective nest entrances, recognize the hive, and collect; S4: After raising for a period of time, adjust the aperture of the queen excluder to 0.2 cm and spray a bee colony odor reduction liquid; S5: After 3 to 5 days, adjust the aperture of the queen excluder to 0.38 cm; S6: After a period of time, remove the escape prevention piece on the nest entrance of the Chinese bee colony to make it more free for the Chinese bees to enter and exit; S7: Add a super in the season with sufficient honey and pollen sources. The present invention provides a method for mixed feeding of Italian bees and Chinese bees, which can not only improve the mite resistance of the Italian bee colony, but also reduce the risk of the Chinese bee colony being infected with wax moths.

This invention discloses a method for improving the fruit set rate of Hovenia dulcis, belonging to the field of agricultural production technology. Addressing the low fruit set rate caused by anther abortion, insufficient pollinating insects, and self-incompatibility, this invention comprehensively applies three synergistic measures: First, spraying a hormone solution of GA 35 μg / g and IAA 3 μg / g during the bud stage promotes pollen and style development; second, attracting native bees by planting motherwort and white clover, and leasing and introducing Italian bee colonies during peak flowering to enhance natural pollination; finally, preparing and spraying an artificial pollination solution containing specific concentrations of sucrose, boric acid, and calcium ions during the flowering period for assisted pollination. This method systematically solves the bottlenecks in each stage of pollination, increasing the fruit set rate of Hovenia dulcis from a natural 12.67% to a maximum of 71.24%, resulting in a significant yield increase and wide applicability to the development of the Hovenia dulcis industry.

According to the method for promoting spawning by feeding the Italian bee queen with juvenile hormone, the Italian bee queen is quantitatively fed with a JHIII sugar aqueous solution with the concentration of 500 micrograms / milliliter, each queen bee is fed with 20 microliters of JHIII sugar aqueous solution, that is, the effective mass of JHIII fed to each queen bee is 10 micrograms, and the result shows that after the method is adopted, the content of the JHIII sugar aqueous solution in the Italian bee queen is increased, and the content of the JHIII sugar aqueous solution in the Italian bee queen is increased. The egg laying quantity and quality of the Italian bee queen bee are remarkably improved, and meanwhile the Italian bee queen bee can still keep a good physiological state.

The invention discloses a method for increasing the comb making rate of a Chinese bee movable frame with a foundation, which comprises the following steps of: S1, selecting a Chinese bee and Italian bee colony with good colony potential and normal spawning of a queen bee, and feeding the Chinese bee and Italian bee colony in a beehive; s2, during sunset, adding a frame for mounting a Chinese honeycomb foundation into a nest of the swarms of Italian bees, and feeding syrup to the swarms of Italian bees; after the apis mellifera constructs a semi-finished honeycomb, the semi-finished honeycomb is taken out; s3, semi-finished honeycombs taken out of the Italian honeycombs are added into the Chinese bee colony nests for Chinese bee colonies to build honeycombs in a relay mode, the Chinese bee colonies are fed with syrup, and spleen building is completed by the Chinese bee colonies. By means of the method, the Chinese bee finished product honeycomb of the Langshi standard box can be built within 28 hours.

The invention belongs to the technical field of honey detection, and particularly relates to a method for identifying Chinese mountain sapium sebiferum honey and Italian mountain sapium sebiferum honey based on the amino acid ratio. The method for identifying the Chinese alpine mountain sapium sebiferum honey and the Chinese alpine mountain sapium sebiferum honey provided by the invention comprises the step of identifying the Chinese alpine mountain sapium sebiferum honey and the Chinese alpine mountain sapium sebiferum honey on the basis of the ratio of the proline content in the total content of free amino acids and / or the ratio of the phenylalanine content in the total content of the free amino acids in a honey sample. The method has the advantages of simplicity and convenience in operation, rapidness, high accuracy and the like, can realize rapid identification of the source of the Sapium discolor honey species, provides technical support for authenticity identification of honey, and has important significance in promoting healthy development of the honey industry.

The application relates to the field of biotechnology, and particularly relates to a MicroRNA primer group for detecting Nosema infection of Apis mellifera, a kit and application thereof. The primer group comprises the following primers: miR-6941-F, miR-6941-R, U6-F and U6-R; the nucleotide sequence of the miR-6941-F is shown in SEQ ID No. 1; the nucleotide sequence of the miR-6941-R is shown in SEQ ID No. 2; the nucleotide sequence of the U6-F is shown in SEQ ID No. 3; and the nucleotide sequence of the U6-F is shown in SEQ ID No. 4. The present application finds and identifies a specific MicroRNA molecule miR-6941 which is significantly changed after Nosema infection of Apis mellifera worker larvae. The primer group provided by the present application can be used for early diagnosis of Italian bee chalkbrood, and the diagnosis time is earlier than that of traditional methods. The primer group can provide a diagnosis basis for whether the larvae of a bee colony are infected with chalkbrood before the larvae are sick, provide a new perspective and target for elucidating the molecular mechanism of Apis mellifera resistance to chalkbrood, and lay a theoretical foundation for future development of a new bee disease prevention and control technology based on RNA interference.