2 results about "Italian bee" patented technology

This invention discloses a method for improving the fruit set rate of Hovenia dulcis, belonging to the field of agricultural production technology. Addressing the low fruit set rate caused by anther abortion, insufficient pollinating insects, and self-incompatibility, this invention comprehensively applies three synergistic measures: First, spraying a hormone solution of GA 35 μg / g and IAA 3 μg / g during the bud stage promotes pollen and style development; second, attracting native bees by planting motherwort and white clover, and leasing and introducing Italian bee colonies during peak flowering to enhance natural pollination; finally, preparing and spraying an artificial pollination solution containing specific concentrations of sucrose, boric acid, and calcium ions during the flowering period for assisted pollination. This method systematically solves the bottlenecks in each stage of pollination, increasing the fruit set rate of Hovenia dulcis from a natural 12.67% to a maximum of 71.24%, resulting in a significant yield increase and wide applicability to the development of the Hovenia dulcis industry.

The application relates to the field of biotechnology, and particularly relates to a MicroRNA primer group for detecting Nosema infection of Apis mellifera, a kit and application thereof. The primer group comprises the following primers: miR-6941-F, miR-6941-R, U6-F and U6-R; the nucleotide sequence of the miR-6941-F is shown in SEQ ID No. 1; the nucleotide sequence of the miR-6941-R is shown in SEQ ID No. 2; the nucleotide sequence of the U6-F is shown in SEQ ID No. 3; and the nucleotide sequence of the U6-F is shown in SEQ ID No. 4. The present application finds and identifies a specific MicroRNA molecule miR-6941 which is significantly changed after Nosema infection of Apis mellifera worker larvae. The primer group provided by the present application can be used for early diagnosis of Italian bee chalkbrood, and the diagnosis time is earlier than that of traditional methods. The primer group can provide a diagnosis basis for whether the larvae of a bee colony are infected with chalkbrood before the larvae are sick, provide a new perspective and target for elucidating the molecular mechanism of Apis mellifera resistance to chalkbrood, and lay a theoretical foundation for future development of a new bee disease prevention and control technology based on RNA interference.