38 results about "Italian bee" patented technology

The invention discloses a method for breeding a high-quality mite-resistant bee species. The method is characterized by comprising the following steps of: (1) selecting breeding materials: screening a 'Jiangshan No.2' Italian bee with a high royal jelly yield, a French pure-bred Italian bee with excellent honey making performance and a healthy local Chinese bee species with excellent mite resistance for serving as an introduction object according to series indexes such as mite resistance, productivity, vitality and the like; (2) breeding a mite-resistant bee species: breeding a breeding male bee from Jiangshan No.2, breeding a Jiangshan No.2 virgin queen bee according to the requirements of bee biology and genetic breeding characteristic, and performing artificial fertilization hybridization with the bred breeding male bee to form a Jiangshan No.2 bee species with high production performance and disease resistance for serving as a male parent, wherein a pure-bred Italian bee with excellent honey making performance introduced from France serves as a female parent; and (3) checking and cutting a French Italian bee drone pupae every 7 days from breeding in spring till the end of breeding. The harm of bee mites to bee colonies can be avoided for beekeepers, the reproduction speed of the bee colonies is increased, and the yields and qualities of bee products are improved.

The invention provides a honeybee breeding method and belongs to the technical field of animal husbandry. The breeding method includes the following steps: firstly, select a bee breeding place, set up a beehive, and regularly apply acaricide to the beehive to remove bee mites; then, select North China bee, Italian bee and Northeast black bee as parents, Breeding bees with stable and excellent properties as bee species; and feeding feed a made of honey, white sugar, orange juice, tomato juice, longan juice, grape juice, citric acid, edible salt, water, and natural pollen, Feed b made of defatted soybean flour, white sugar and appropriate amount of water. The invention provides a honeybee breeding method. The breeding method is safe and reliable. The North China honeybee, Italian honeybee and Northeast honeybee are scientifically bred to obtain a honeybee with high yield, cold resistance, mite resistance and disease prevention. species, and then fed scientifically and rationally to promote the healthy development of bees, reduce the incidence of morbidity, and improve the quality and food safety of honey.

The invention discloses a method for breeding a high-quality anti-mite bee variety. The method is characterized by comprising the following steps of: 1) selecting breeding materials, wherein 'jiangshan No.2' Italian bees with relatively high royal jelly yield, France pure Italian bees with high honey production performance and healthy local Chinese bees with high anti-mite performance are selected as species introduction objects according to a series of indexes such as anti-mite performance, productivity and vitality; 2) breeding the anti-mite bee variety, wherein breeding drones are selected from the jiangshan No.2, selecting a bred jiangshan No.2 virgin queen bee according to the requirements of honey bee biology and inheritance breeding characteristics, and performing the artificial fertilization and cross breeding between the breeding drones and the bred jiangshan No.2 virgin queen bee so as to obtain a jiangshan No.2 bee variety with high production performance and disease resistance, using the jiangshan No.2 bee variety as male parents and using the pure Italian bees which are introduced from France and have high honey production performance as female parents; and 3) since breeding starts in spring, detecting and cutting off pupae of the France Italian bees every other 7 days till the breeding ends. The method can solve the problem that bee colonies are jeopardized by bee mites for beekeepers and improve breeding speed of the bee colonies and the yield and the quality of bee products.

The invention relates to application of biogenic amine serving as a feature mark in bee venom type identification and further relates to a method for identifying types of bee venoms through the biogenic amine as the feature mark by means of HPLC / MS / MS methods. According to the method, one or more of histamine, dopamine, serotonin, epinephrine and noradrenaline serves as the feature mark, one or more of Italian bee venom, royal jelly high-yielding bee venom, Anatolia bee venom, Caucasia bee venom, Carniolan bee venom and Carpathian bee venom can be precisely identified. The method can efficiently, stably and accurately identify six types of bee venoms, and has significance on reinforcing study on bee venom type identification, exploring bee venom traceability information, developing traditional Chinese medicine, improving bee venom quality in China and guaranteeing health of consumers.

The invention discloses a method for improving the yield and quality of Chinese bee honey. The Chinese bee has the advantages of capability of taking advantage of sporadic nectariferous plants, strong gathering power, high utilization efficiency, long period of honey gathering, high adaptability, and high resistance to mites and diseases. While, the Italian bee is larger than the Chinese bee and docile, has strong spawning and feeding capacity, is able to suddenly gather a large quantity of nectar sources, and has strong bleeding capacity. The method comprises the steps of putting the honeycomb spleens of raised Chinese bees in the bee hive of raised Italian bees to feed the Italian bees for 1-2 days and taking the honeycomb spleens back to the bee hive of the Chinese bees, wherein the honeycomb spleens contain Chinese bee egg spleens. The method combines the advantages of the Chinese bee and the Italian bee. Through Italian bee feeding, the adult Chinese bee is strong, the gathering and bleeding abilities of the Chinese bee are improved, and the yield and quality of honey are improved. The produced honey is deep yellow, has a unique taste, is mellow, and has high concentration, and the honey is sweet and delicious, and is of rich nutrition.

The invention discloses a hybrid queen bee breeding method. The hybrid queen bee breeding method comprises the steps that (1), eggs produced by Italian queens are incubated for three days in a beehiveinto larvae, queen cells are made with beewax, the incubated Italian larvae are transferred to the queen cells respectively and then placed into a Chinese bee box to be subjected to Italian bee breeding, and the Italian larvae get involved in Chinese bee ingredients; (2), eggs produced by the Chinese bees are incubated for three days in the beehive into larvae, the larvae are transferred to the queen cells and then placed into an Italian box to be subjected to Chinese bee breeding, and the Chinese bees get involved in Italian bee ingredients; (3), the Italian larvae in the Chinese bee box arebred to become Italian bee female queens, and the Chinese bee larvae in the Italian bee box are bred to become Chinese bee male queens; (4), copulation is conducted, the born Italian bee female queens are guided to copulate and spawn with the Chinese bee male queens mutually, and after 20 days of incubation, hybrid bees are bred. By the adoption of the hybrid queen bee breeding method, the bred bees combine advantages of the Italian bees and the Chinese bees.

The invention relates to a method for verifying anti-chalk disease traits of a swarm by an SNP marker. The method comprises the following steps: collecting larva samples from Italian swarms to be observed, extracting larva DNA, synthesizing a target primer, carrying out PCR with each extracted individual DAN as a template, carrying out electrophoresis detection on a PCR product, sequencing, finally comparing and analyzing the obtained sequencing result, and verifying the anti-chalk disease traits by judging whether the occurrence frequency PC of a C allele of randomly captured larva individuals from the swarm has difference with the occurrence frequency PT of a T allele in the SNP marker C2587245T. According to the invention, aiming at the problem that the Italian swarms are severely affected by the chalk disease, the SNP (C2587245T) marker related to anti-chalk disease of Italian bee larva provided from the molecular biology level for scientifically, accurately and quickly verifying the strength of the anti-chalk disease performance of the Italian swarms, the breeding cycle of the anti-chalk disease breeding of the Italian bees can be greatly shortened, the bee breeding speed is accelerated and the loss in the beekeeping industry caused by the chalk disease is reduced.

The invention discloses a method for cultivating a hybrid queen bee, the steps of which are as follows:) hatch eggs produced by the Italian queen bee in a beehive for 3 days to hatch into larvae, use beeswax to make a queen bee, and transplant the hatched Italian bee larvae to the queen bee respectively , and put into the Chinese honeybee hive for Italian bee cultivation, so that the Italian bee larvae intervene in the Chinese bee ingredients; 2) use the eggs produced by the Chinese bee to hatch in the beehive for 3 days to hatch into larvae, move into the royal platform, put into the Italian bee box and enter the Chinese honeybee 3) Cultivate the larvae of the Italian bee in the Chinese bee box to become the female queen bee of the Italian bee; cultivate the larvae of the Chinese bee in the Italian bee box to become the male queen bee of the Chinese bee;) Mating, the Italian bee will be born The female queen bee is introduced to mate with the Chinese bee drone queen bee, lay eggs, and after 20 days of incubation, hybrid bees are cultivated. The honeybees cultivated by the method combine the advantages of both the Italian honeybee and the Chinese honeybee.

The invention relates to a method for identifying a swarm royal jelly high yield character with an SNP mark rs16287910. The method comprises the steps of bee individual sampling, bee sample DNA extraction, primer synthesis, PCR detecting and swarm royal jelly high yield character identifying. According to the fact whether obvious difference exists between the frequency PT of which a T allele appears at the SNP mark rs16287910 of worker bee individuals collected randomly in a swarm and frequency PC of a C allele, the swarm royal jelly high yield character is identified. From the level of molecular biology, the SNP(rs16287910) mark related to the Italian bee worker bee individual royal jelly high yield is provided, the Italian bee worker bee individual royal jelly high yield character is identified scientifically, accurately and quickly, the breeding cycle of royal jelly high-yield Italian bees can be greatly shortened, and bee breeding speed is increased.

The invention discloses a method for detecting 6-pyruvoyl tetrahydropterin synthase PTS gene expression of Italian bee through a fluorescent quantitative PCR (Polymerase Chain Reaction) technology andparticularly relates to the technical field of biology. A real-time fluorescent RT-PCR detection method for PTS of the Italian bee is established, and the method comprises the following steps: (1) performing gene detection and acquisition by using primers; (2) extracting total RNA; (3) synthesizing cDNA; (4) real-time fluorescent PCR; (5) performing relative expression calculation of PTS genes ofworker bee, drone and queen bee in the Italian bee. Compared with the conventional PCR, the real-time fluorescent RT-PCR adopted in the invention has the advantages that fluorescence signals are collected by an automatic instrument in the real-time fluorescent PCR technology, the subjectivity of judgment by naked eyes is avoided, and the sensitivity is further improved. The real-time fluorescentPCR technology is in a totally closed reaction, PCR after-treatment is not needed, pollution is avoided, and the reliability and repeatability of the results are ensured. Moreover, electrophoresis, quantitative scanning and other subsequent complicated steps in the conventional PCR are eliminated, and the experimental time is greatly shortened.

The invention discloses a Chinese bee queen breeding method which includes the steps: feeding Chinese bee queen with royal jelly by the aid of young Italian bee swarms which serve as feeding swarms, and wherein the Chinese bee queen is Chinese bee queen after natural mating; the weight and the daily egg laying amount of the Chinese bee queen are remarkably increased, the egg weight, the egg lengthand the birth weight of offspring worker bees are increased to different extents, the shapes of the offspring worker bees are changed to different extents, and the reproductive performance of the Chinese bee queen bee is improved.

A method for the production of hybrid seeds for the medical valerian plant, which grows in the high altitude area. The method comprises the following steps: choosing the artificial cultivating field for valerian plant which is in shed or not with good drainage as the field for the production of hybrid seeds; introducing bees into the field when the amount of flower is 25%, the number of bees is 15-20 / m2; covering the plant with bags when 90% flowers withered; recovering the seeds within one or two weeks after all inflorescence withered and before plants begin fall to ground; sifting the seeds and getting seeds with cleanliness above 95%. In shed, Italian bees are employed, while out of shed are china bees. The invention employs bees as pollination medium and the germination rate is increased from below 30% to above 70%, thus solving the problem of seed supply for large-area of artificial cultivating of the said genus; by applying bees for pollination, the method can realize the effect of labor and time saving.

The invention discloses a building method of an Apis cerana-Apis mellifera ligustica mixed colony model. The method includes: preparing a bee hive, and placing a gauze element in the middle of the beehive to separate the bee hive into a left independent space and right independent space; preparing a healthy Apis mellifera ligustica colony and a healthy Apis cerana colony, and respectively placingthe healthy Apis mellifera ligustica colony and the healthy Apis cerana colony into the two independent spaces; opening two hive doors at the same time to allow the bees in two independent spaces tobe able to freely go in and out the hive doors; taking honey from the honeycombs of the Apis cerana and the Apis mellifera ligustica, mixing the honey, and smearing the gauze element with the mixed honey 3-5 times a day for 5-10 days; after 3-5 days, observing the acceptance of the Apis mellifera ligustica to the honeycomb of the Apis cerana, and immediately removing the gauze element if the Apismellifera ligustica accepts the honeycomb of the Apis cerana; continuing smearing the gauze element with the mixed honey if the Apis mellifera ligustica does not accept the honeycomb of the Apis cerana until the Apis mellifera ligustica accepts the honeycomb of the Apis cerana. By the method, Apis cerana larvae can be prevented from being eliminated by the Apis mellifera ligustica, and the uncovered Apis cerana larvae in the mixed bee colony can be normally cultured.

The invention provides a method for breeding pyemotes and a method for preventing and treating boring pests by using the pyemotes. The method for breeding the pyemotes comprises the steps: (1) preparing a first generation of artificial pyemotes species by using larvae and pupae of Chinese bees or Italian bees; (2) making mass breeding species by using the larvae and the pupae of the Chinese bees or the Italian bees; (3), carrying out artificial large-scale breeding of the pyemotes by using the larvae and the pupae of the Chinese bees or the Italian bees ; and (4) packaging and storing. According to the method for breeding the pyemotes, the larvae and the pupae of the Chinese bees or the Italian bees are used as substitute hosts of the pyemotes, so that the breeding cost of the pyemotes can be greatly reduced, the breeding quantity of the pyemotes can be increased, the high activity of the pyemotes species can be kept, and the artificial large-scale breeding of natural enemies of the pyemotes can be really realized; when the pyemotes obtained by the method are used for preventing and treating the boring pests, the pyemotes have a good prevention and treatment effect on the boring pests.

The invention discloses leguminous plant hybridization cultivated fields, and belongs to the field of plant cultivation. The leguminous plant hybridization cultivated fields are mainly suitable for cultivation of common mung beans and red beans and comprise a cultivated field A, a cultivated field B and a breeding field, wherein the distance between the cultivated field A and the cultivated field B is 40 m, and the cultivated field A and the cultivated field B are arranged side by side and located on the position of 40 m in the south of the breeding field; the cultivation process comprises the steps that 1, 300 strains of the mung beans and 50 strains of peas are selected to be mixedly cultivated in the cultivation field A, and 200 strains of high-quality small red beans and 50 strains of cowpeas are selected to be mixedly cultivated in the cultivated field B; 2, 30 boxes of Italian bees and 18 boxes of northeastern black bees are mixedly bred in the breeding field; 3, after beans are harvested, the beans of which the size is two times that of the common beans are selected to be separately collected and stored; 4, the selected beans are sown after Grain Rain in spring. The mung beans and the small red beans which are cultivated through the leguminous plant hybridization cultivated fields belong to pure natural hybrid products, the yield is increased by 2-3 times compared with common cultivation, pest and disease resistance is high, management is convenient, the cultivated beans are full, the taste is good, and the nutritional value is rich.

The invention relates to the field of plant breeding, in particular to an insect pollination method for different entomophilous hermaphrodite flower herb pollination groups. According to the method, asteel frame arched shed serves as a basic facility, the roof of the shed is covered with a plastic film, the specification of a sieve is set according to the scale of the pollination groups (a singleplant, opposite cross and testcross groups, small parent or stock and breeding material groups and large parent or stock groups), corresponding isolation units are built, artificial pollination is changed into insect pollination, pollination insects can include osmia, bumblebees, Italian bees and Chinese bees, and rain sheltering measures are implemented during flowering pollination. According tothe method, artificial pollination cannot be depended, reproduction of cross parents can be implemented, the yield of seeds is low under artificial pollination, the yield can be remarkably improved under insect pollination, isolation area entering and exiting frequency of workers is remarkably reduced when management is reasonable, and the probability of pollination mixing between materials can be effectively reduced. Besides, huge labor cost of insect pollination is saved as compared with artificial pollination.

The invention discloses an efficient Chinese bee breeding device and breeding method, and relates to the technical field of artificial breeding of bees. Comprising the steps of queen breeding population selection, queen cell preparation, queen breeding without larva transferring, queen bee feeding, rapid breeding and the like, and meanwhile, by arranging an efficient breeding device, the queen bee quality is improved and breeding is promoted by adopting an external direct supplementary feeding and pheromone supplementing mode. The method overcomes the defects in the prior art, queen bee larvae are fed with Italian bee royal jelly, the quality of a new queen bee is improved through nutritional hybridization, meanwhile, larva pheromone and pupa pheromone are properly supplemented in the breeding process, the temperature of a nest box is effectively adjusted, the collecting and feeding efficiency of worker bees and the egg laying efficiency of the queen bee are improved, and the survival rate of the queen bee is increased. Therefore, the bee colony breeding is promoted, the honey yield and pollination efficiency of the Chinese bees are further improved, and a foundation is laid for bee farmers to increase both production and income.

The invention discloses a method for establishing a mixed colony model of Apis mellifera and Apis mellifera, which comprises the following steps: preparing a beehive, using gauze to separate the middle part of the beehive into two independent spaces on the left and right; preparing a colony of healthy Italian bees and healthy bees group, put them into two independent spaces respectively; open two hive doors at the same time, so that the bees in the two independent spaces can freely enter and exit the hive door; get honey and mix respectively from the combs of the middle bees and Italian bees, and mix The mixed honey is evenly spread on the gauze of the beehive, smeared 3-5 times every day, and continuously smeared for 5-10 days; The gauze is removed; if it is not accepted, continue to smear honey on the gauze until the Chinese bee and the Italian bee accept each other. The method effectively avoids the cleaning behavior of the Italian bees on the bee broods of the middle bees, and enables the uncapped bee broods in the mixed bee colony to be raised normally.

The invention discloses a method for detecting expression of an odorant-binding protein gene OBP6 of Italian bees by using fluorescent RT-PCR technology, and particularly relates to the technical field of biology. The method for detecting the expression of the odorant-binding protein gene OBP6 of the Italian bees by using the fluorescent RT-PCR technology comprises the following steps that (1), OBP6 genes are detected by using primers; (2), total RNA extraction is performed; (3), cDNA synthesis is performed; (4), real-time fluorescent PCR is performed; and (5), the relative expression quantityof OBP6 of the Italian bees is calculated before and after benomyl treatment. The real-time fluorescent RT-PCR adopted by the method for detecting the expression of the odorant-binding protein gene OBP6 of the Italian bees by using the fluorescent RT-PCR technology is compared with conventional PCR, and the real-time fluorescent PCR technology collects fluorescent signals by automated instruments, the subjectivity of visual judgment is avoided, and the sensitivity is further improved; and at the same time, the real-time fluorescent RT-PCR technology reacts in a fully closed mode without post-treatment of PCR, pollution is avoided, and the reliability and repeatability of the results are ensured. At the same time, the following tedious steps such as electrophoresis and quantitative scanning in routine PCR are eliminated, and the experiment time is greatly shortened.

The invention relates to the field of biotechnology and immunodetection, in particular to an anti-apis mellifera MRJP1 monoclonal antibody and a colloidal gold detection card. The invention provides ahybridoma cell strain 1F 61D 7 and a hybridoma cell strain 6F 124B 4 which stably secrete the anti-apis mellifera MRJP1 monoclonal antibody, the secreted antibody only recognizes apis mellifera MRJP1protein, does not recognize apis cerana MRJP1 protein, has the advantages of good specificity, high sensitivity and the like, and has no cross reaction with other family related proteins. The colloidal gold detection card and test strip of the apis mellifera MRJP1 are further developed from the antibody, the colloidal gold detection card and test strip have the advantages of high precision, smallbatch difference and the like, rapid detection of the apis mellifera MRJP1 in a bee product is achieved, and the colloidal gold detection card and test strip are particularly suitable for high-efficiency and large-batch detection of whether apis mellifera honey is mixed into Chinese honey (local honey) or not, and are particularly suitable for high-efficiency and large-batch detection of whetherapis mellifera honey is mixed into Chinese honey (local honey) or not

Particulally relating to the field of biotechnology, the invention discloses a method for detection of Italian bee immunogene defensin-1 expression by fluorescence RT-PCR technology. Establishment of the Italian bee immunogene defensin-1 real-time fluorescence RT-PCR detection method comprises the steps of: (1) gene detection and acquisition with primers; (2) extraction of total RNA; (3) cDNA synthesis; (4) real-time PCR; and (5) calculation of the Italian bee defensin-1 relative expression level before and after benomyl treatment. The invention lays the foundation for study of Italian bee defensin-1 expression regulation mechanism and immunological functions thereof, the disease-resistant molecular mechanism of Italian bees, scientific utilization of antibiotics to ensure safety production of bee products and disease control of bees, study of spatial and temporal expression condition of Italian bee immunogene defensin-1 and the influence of antibiotic benomyl feeding on the Italian bee immunogene defensin-1 expression level, and provides a technical platform for defensin-1 gene identification on the mRNA level and relative quantitative analysis.

The invention relates to a method for identifying a swarm royal jelly high yield character with an SNP mark rs16287910. The method comprises the steps of bee individual sampling, bee sample DNA extraction, primer synthesis, PCR detecting and swarm royal jelly high yield character identifying. According to the fact whether obvious difference exists between the frequency PT of which a T allele appears at the SNP mark rs16287910 of worker bee individuals collected randomly in a swarm and frequency PC of a C allele, the swarm royal jelly high yield character is identified. From the level of molecular biology, the SNP(rs16287910) mark related to the Italian bee worker bee individual royal jelly high yield is provided, the Italian bee worker bee individual royal jelly high yield character is identified scientifically, accurately and quickly, the breeding cycle of royal jelly high-yield Italian bees can be greatly shortened, and bee breeding speed is increased.

The invention relates to a method for identification of bee colony royal jelly high yield characters by SNP (single nucleotide polymorphism) marker rs4208349. The method includes: bee individual sampling, bees sample DNA extraction, primer synthesis, PCR detection and identification of bee colony royal jelly high yield characters. For the bee individuals randomly collected from the bee colony, according to whether significant difference exists between the occurrence frequency PA of A allele at the SNP marker rs4208349 and the occurrence frequency PG of G allele, the bee colony royal jelly high yield characters can be identified. From the molecular biological level, the invention provides the method using the apis mellifera worker bee individual royal jelly high yield related SNP (rs4208349) marker to scientifically, accurately and rapidly identify the apis mellifera colony royal jelly high yield characters, can greatly shorten the breeding cycle of high royal jelly producing apis mellifera species, and accelerates the bee breeding speed.

The invention provides an ELISA detection kit for detecting Apis cerana Smith honey and Apis mellifera honey. The MRJP2(major royal jelly protein 2) is selected as the target protein for Apis cerana Smith honey and Apis mellifera honey detection, the Apis cerana Smith MRJP2 and the Apis mellifera MRJP2 are obtained through a Prokaryotic expression purification, and preparing and purifying to obtain the specificity and identity antibody of the Apis cerana Smith MRJP2 and the Apis mellifera MRJP2, and the Apis cerana Smith honey and the Apis mellifera detection kit is developed by using a doubleantibody sandwich method. The ELISA detection kit disclosed by the invention has high sensitivity, the developing condition can be observed through the naked eyes, 1% of Apis mellifera honey doping in the Apis cerana Smith can be identified within 45min, the convenient and fast detection requirement is satisfied, and the detection kit is a reliable tool for Apis cerana Smith honey and Apis mellifera honey detection.

The invention provides a detection method and application of Italian bee body color differentiation related gene, and relates to the technical field of biology. The detected related gene is the gene of the Italian bee pteridin-4-alpha-methanol amine dehydratase, and the detection method mainly comprises the following steps: designing fluorescent quantitative primers Amel-PCD-QRT-F and Amel-PCD-QRT-R, extracting total RNA, synthesizing cDNA, carrying out real-time fluorescent PCR, and calculating gene expression. The defects in the prior art are overcome, a related reference index of the synthesis amount of BH4 in bees is established by detecting pteridin-4-alpha-methanol amine dehydratase PCD, and a theoretical basis is provided for the synthesis route of BH4 of Italian bees by researching the gene expression level of PCD of bees of various differentiation types in an Italian bee colony; and a certain reference is provided for researching a pigment metabolism network in a bee body.