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A method for identification of high yield of royal jelly in bee colonies using snp marker rs16287910

A high-yield technology of bee colony royal jelly, which is applied in the field of identification of high-yield traits of bee colony royal jelly and identification of high-yield traits of bee colony royal jelly, can solve the problems of unfavorable popularization, complicated identification process, high cost, etc., and achieve improved success rate, rapid identification, and improved efficiency effect

Inactive Publication Date: 2017-12-08
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

The more reliable ones are the three molecular markers screened by Pan Jiao et al. (2012) using gene chip technology within the range of the honeybee genome. However, this method requires the extraction of honeybee RNA and the use of fluorescent quantitative instruments, which makes the identification process complicated and expensive. , which is not conducive to the popularization of the method

Method used

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  • A method for identification of high yield of royal jelly in bee colonies using snp marker rs16287910
  • A method for identification of high yield of royal jelly in bee colonies using snp marker rs16287910
  • A method for identification of high yield of royal jelly in bee colonies using snp marker rs16287910

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Embodiment 1

[0039] A method for identifying high-yield traits of bee colony royal jelly using SNP marker rs16287910, comprising the following steps:

[0040] (1) Sampling of bees: 50 adult worker bee individuals were collected from the bee species with high royal jelly production ("Zhenongda No. 1") and the bee species with low royal jelly production (Apis mellifera, USA), and each individual was placed in a centrifuge tube. Freeze at -20°C for later use;

[0041] (2) Extraction of DNA from bee samples (using Sangon’s Animal Genome Rapid Extraction Kit, SK8222)

[0042] Use ophthalmic scissors to cut off the limbs, wings and abdomen of the individual worker bees in step (1), keep only the head and thorax, put them in a mortar filled with liquid nitrogen, crush them with a grinding rod, and then transfer them to a 1.5 mL centrifuge tube Add 400 μL Buffer Digestion, shake evenly, and place in a water bath at 65 °C for 1 h until the cells are completely lysed. Then remove the supernatant...

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Abstract

The invention relates to a method for identifying a swarm royal jelly high yield character with an SNP mark rs16287910. The method comprises the steps of bee individual sampling, bee sample DNA extraction, primer synthesis, PCR detecting and swarm royal jelly high yield character identifying. According to the fact whether obvious difference exists between the frequency PT of which a T allele appears at the SNP mark rs16287910 of worker bee individuals collected randomly in a swarm and frequency PC of a C allele, the swarm royal jelly high yield character is identified. From the level of molecular biology, the SNP(rs16287910) mark related to the Italian bee worker bee individual royal jelly high yield is provided, the Italian bee worker bee individual royal jelly high yield character is identified scientifically, accurately and quickly, the breeding cycle of royal jelly high-yield Italian bees can be greatly shortened, and bee breeding speed is increased.

Description

technical field [0001] The invention relates to a method for identifying high-yield traits of bee colony royal jelly, in particular to a method for identifying high-yield traits of bee colony royal jelly by using SNP marker rs16287910, and belongs to the field of biotechnology. Background technique [0002] Royal jelly has many important nutritional and medicinal values, is very beneficial to human health, and is a natural nourishing food. my country is a big beekeeping country, and the output of royal jelly accounts for more than 90% of the world's total, which has an inseparable relationship with the bee species raised in our country. Our country's bee industry scientists and scientific and technical personnel have bred "Zhenongda No. 1" and Xiaoshan jellyfish through the closed breeding method of bee groups. , Has become my country's unique and main bee species. For a long time, my country's bee industry scientific and technological workers have been committed to the res...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 苏松坤刘小彦李志国许叔鹏孟魏宏
Owner FUJIAN AGRI & FORESTRY UNIV
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