34 results about "Worker bee" patented technology

The utility model discloses a device for artificial feeding of worker bees, which comprises a beehive body, a beehive cover movably connected to the top of the beehive body, and a support plate movably connected to one side of the beehive body. The device solves the problem of mutual cooperation of the support plate, sugar storage pot, cover plate, connecting pipe, feeding box, mounting plate, nut, protruding point and climbing net, so that the worker can feed the worker bees without opening the beehive body to add honey water. The worker can determine the capacity of the feeding box by the capacity of the sugar storage pot according to the principle of communicating vessel, which makes the use more convenient and improves the practicability of the device. The cooperation of the protruding point and climbing net provides a standing point for the worker bees inside the device when they eat, which makes the eating more safe and further improves the practicability of the device.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to the body size and temperature adaptability of worker bees of apis cerana and application of the SNP marker. According to the invention, 25 key SNP sites and 10 associated genes for regulating and controlling the body type and temperature adaptation of the apis cerana are screened by a selection signal analysis method. Research finds that in the second intron region (6452066th site of the chromosome 7 of the bees) of the Foxo gene, the mutation type is T / C, and the mutation type is closely related to the body size and temperature adaptability of the Chinese bees. Through verification, in Hainan apis cerana and Aba apis cerana with extremely different body sizes and living environment temperatures, mutation position genotypes are different, TT is a large-body cold-resistant dominant genotype, and CC / TC is a small-body heat-resistant dominant genotype; a gene expression quantity detection result shows that the expression quantity of the Foxo gene is in negative correlation with a large body type and cold resistance and is in positive correlation with a small body type and heat resistance. The method can be used for genetic improvement of bee body shape characters and has breeding application value.

The utility model discloses a multifunctional beehive entrance, and belongs to the technical field of bee breeding. The hive entrance body is in a round cake shape and is divided into a front face and a back face, the hive entrance body is evenly divided into four different functional areas including a normal access area, an anti-escape and anti-pollen-falling area, a ventilation area and a closing area through two perpendicularly-intersected reinforcing ribs, screw holes are reserved in the intersection center points of the reinforcing ribs, and protruding frames are designed on the edges of the front face and the back face of the hive entrance. The rotary handle and the lifting platform are arranged on the front side; the bee hive door can defend natural enemies and solve the problems that a bee colony is prone to escape during swarming, worker bees are difficult to collect powder and return to the bee hive, powder falls off easily and the like, the four functional areas are scientifically sequenced, when two adjacent functional areas correspond to holes of the bee hive at the same time, specific requirements in special scenes can be met, and protrusions are arranged on the frame of the bee hive door and the edges of all functional areas. The hive entrance is tightly attached to the beehive, and friction force during rotation can be reduced. By means of the design, the working efficiency and the yield of bees can be greatly improved.

The invention relates to the field of economic animal feeding, and particularly discloses a technical method for promoting efficient and healthy breeding of bumblebees based on circadian rhythm, which comprises the following steps: breaking the diapause state of queen bees by using high-concentration COs, recovering the activity of the queen bees, transferring the recovered queen bees into an early-stage queen bee nesting feeding box, and providing normal pollen and liquid food; putting the queen bee breeding box into an incubator, simulating a natural photoperiod environment at the temperature of 28-29 DEG C and the humidity of 50-60%, and helping the queen bee to adapt to the circadian rhythm; at the beginning, the feeding amount of the queen bees every day is equal to the size of soybeans, and after the first batch of worker bees are hatched, the queen bees, the worker bees and honeycombs are transferred into a larger feeding box; according to the method, the biological clock of the queen bee is adjusted by controlling time service factors such as illumination, temperature, humidity and food, the survival rate of the queen bee is remarkably increased, the growth and development of the queen bee and the health state of a swarm are improved, and the technical method for promoting efficient and healthy breeding of the bumblebees based on the circadian rhythm is established.

The utility model provides a multipurpose queen bee parturition control queen bee isolation grid device which is provided with two grid holes, the first grid hole is a first grid hole with the fixed width, and the second grid hole is a second grid hole with the adjustable width. Under the condition that the individual size of a queen bee of one kind of bees is close to that of worker bees of the other kind of bees, the device can be universally used for the two kinds of bees, the queen bee can be closed without hindering the worker bees from feeding, and the device is easy to operate and conforms to the habits of the worker bees.

The present invention relates to the field of bumblebee handling in particular in relation to bumblebee rearing, storage, transport and at the pollination location. For these purposes the invention according to a first aspect provides a housing, such as a nest box, for bumblebee (Bombus spp.) individuals, including bumblebee workers. Further aspects of the invention relate to the use of the housing for confining bumblebees and a method of pollinating blooming plants comprising providing in the vicinity of the plants a number of bumblebee housings according to the invention.

The utility model provides a kind of active frame soil bee-keeping box based on standard box improvement, including standard frame, gauze cover, box cover and hollow heightening frame, heightening frame is stacked in standard frame top and the same as standard frame appearance size, and the top of two long side walls of heightening frame is equipped with overhead position, and multiple bee-keeping upside-down nest frames are arranged in overhead position, and guiding nest spleen is fixedly connected in the nest spleen fixed groove of the inner side of the upper crosspiece of each bee-keeping upside-down nest frame, gauze cover is placed in heightening frame top, and box cover is pressed on gauze cover, and breathable hole is arranged on the two wide side walls of box cover. The utility model adds a heightening frame on the top of the existing standard frame, and multiple upside-down nest frames for bee-keeping are arranged on the top of the two long side walls of the heightening frame, which changes the nest spleen into horizontal breeding, so that the worker bees build spherical spleen on the nest spleen, and it will not cause rotten eggs.

The application discloses a honeybee attracting composition of Apis cerana and application thereof, and the honeybee attracting composition of Apis cerana comprises the following raw material components in parts by weight: 0.1-1 parts of queen pheromone of worker bees, 0.1-1 parts of body surface hydrocarbon compounds of worker bees, 0-1 parts of honeycomb extract, and 0-1 parts of extract of a flower spike of a lily. The application finds the principle of attracting swarm bees by lily and simulating swarm pheromone of Apis cerana, and completely analyzes the whole composition of the swarm pheromone of Apis cerana for the first time, and simulates the swarm pheromone of Apis cerana completely by artificial synthesis. It is proved by experiments that the synthesized honeybee attracting composition of Apis cerana has higher activity of attracting swarm bees than that of extracting natural pheromone.

The application discloses a kind of Apis cerana cerana male bee breeding device and method, including top open nest box and the box cover for sealing the top of the nest box, it is characterized in that: the middle part of the nest box is provided with vertical intermediate column, the four sides of the intermediate column are provided with slot A along the axial direction, the inner side of the four side plates of the nest box is respectively provided with vertical slot B, the slot A and the slot B between opposite are provided with the barrier plate that can be inserted and extracted, four barrier plates divide the nest box into four areas and are respectively A area, B area, C area and D area;The A area, B area, C area and D area are respectively provided with nest door, and the opening direction of four nest doors is different;Adjuster is arranged on the barrier plate, and the adjuster can adjust the barrier plate to be closed state, breathable state and only for worker bee to pass through state. The male bee is bred by using the above breeding device and by means of rotation nursing, and the Apis cerana cerana male bee with large quantity and good quality can be quickly bred.

The invention relates to the technical field of biology, and provides an application of a connexin signal channel in regulating and controlling the body weight and the body shape of worker bees. According to the invention, a cascade regulation pathway of Jhe-JH-Titin in bees is disclosed for the first time, which indicates that Jhe (juvenile hormone esterase) regulates JH (juvenile hormone) and further regulates the expression of Titin (connexin) to influence the growth and development of bees, and finally regulates the body type and weight of worker bees of bees. By means of the method, the purposes of regulating and controlling the birth weight and the body type of worker bees can be achieved. According to the method, molecular breeding work is carried out by adopting the regulation and control effect of the Jhe-JH-Titin pathway on the body type of the bees, the traditional breeding period can be shortened, and the breeding target of improving the body type of the bees can be rapidly achieved. The method is simple, convenient, easy to operate and short in breeding period, the body length of worker bees can be rapidly increased only through one generation, the breeding cost can be effectively reduced, and the method has important application and popularization value for bee breeding.

The invention relates to the technical field of biology, in particular to an lncRNA primer group for detecting apis cerana infected by bee glomus and application of the lncRNA primer group, and the primer group comprises the following primers: lncRNA6470-F, lncRNA6470-R, Ace-actin-F and Ace-actin-R; the nucleotide sequence of the lncRNA6470-F is as shown in SEQ ID No. 1 (sequence identifier number 1); the nucleotide sequence of the lncRNA6470-R is as shown in SEQ ID No. 2; the nucleotide sequence of the Ace-actin-F is as shown in SEQ ID No. 3; and the nucleotide sequence of the Ace-actin-R is as shown in SEQ ID No. 4. The research finds and identifies a specific lncRNA molecule, namely lncRNA6470, of which the expression is obviously changed after worker bee larvae of apis cerana are infected by bee glomus, the primer group provided by the research can be used for early diagnosis of the apis cerana chalkbrood disease, the diagnosis time is earlier than that of a traditional method, a diagnosis basis can be provided for whether bee colony larvae are infected by chalkbrood disease or not before the larvae are attacked, and the method has the advantages that the method is simple and convenient to operate, and the cost is low. A new view angle and a new target spot are provided for clarification of a molecular mechanism of Chinese bee chalkbrood disease resistance, and a theoretical foundation is laid for development of a novel bee disease prevention and control technology based on RNA interference in the future.

The application discloses a device and method for improving the egg production of a honeybee colony, wherein the lower left and right sides of the front side plate of a nest box are provided with an outer left nest door and an outer right nest door, and a fixed middle partition plate is arranged in the middle of the nest box, which divides the nest box into a left breeding area and a right breeding area; an active left partition plate is arranged in the left breeding area, which divides the left breeding area into a left queen bee breeding area and a left worker bee swimming area, and the lower part of the active left partition plate is provided with an inner left nest door; an active right partition plate is arranged in the right breeding area, which divides the right breeding area into a right queen bee breeding area and a right worker bee swimming area, and the lower part of the active right partition plate is provided with an inner right nest door, the outer left nest door is located in the left worker bee swimming area, the outer right nest door is located in the right worker bee swimming area, the top of the left queen bee breeding area is provided with an inner left cover plate, and the top of the right queen bee breeding area is provided with an inner right cover plate; a nest box is further stacked with a secondary nest box, and the lower part of the front side plate of the secondary nest box is provided with a secondary nest door. The application can realize the feeding of double queen bees in the same nest box throughout the year.

The breeding method comprises the following steps: collecting larva morphology, royal jelly liquid level and worker bee activity images in a queen cell, and matching a queen bee state evaluation model and a worker bee feeding behavior prediction model; key information such as larva body length, body width, royal jelly liquid level height and worker bee gathering density is extracted through image data, queen bee satiation degree and worker bee feeding intensity are quantitatively evaluated through a model, specific optical signals are generated, worker bees are accurately guided to adjust feeding behaviors, it is avoided that the royal jelly in a queen cell is increased by more than 0.5 mg or reduced by more than 0.3 mg within 1 h, and the survival rate of the queen cell is improved. The satiation degree of the queen bee is stabilized in a healthy interval of 60%-80%; compared with the prior art, monitoring of the queen bee state in queen bee breeding is enhanced, corresponding adjustment is carried out, and the problem that the queen bee breeding quality is low due to visual communication obstacles is solved.

The invention relates to the field of crop pollination, and particularly discloses a method for promoting efficient pollination of bumblebees based on circadian rhythm, which comprises the following steps: preparation of bumblebee colonies: a normal bumblebee colony, a liquid food a containing a queen bee and a certain number of worker bees, and normal pollen b for ensuring the health of the bumblebee colony are needed; putting the beehive into an incubator for domesticating the circadian rhythm, and maintaining the environmental conditions of the bee colony; the number of worker bees in a swarm is 60-80, the bee vigor is determined according to the area of a greenhouse, and the worker bees are moved to a buffer chamber at the temperature of 20 DEG C to be pretreated for 2-3 days before being put into the greenhouse for pollination; after one week, recording the pollination efficiency according to the bite mark rate on the tomato flower, and timely supplementing the feed lacking in the box during the pollination period; on the basis of the original bumblebee feeding conditions, the pollination efficiency of the bumblebees can be greatly improved by adjusting culture of a certain light temperature and feeding time, and the value is improved for better service of the bumblebees to agricultural pollination of greenhouse facilities.

The application relates to the field of crop pollination, and particularly discloses a method for promoting efficient pollination of ground bees based on circadian rhythm, which comprises the following steps: ground bee colony preparation: a normal ground bee colony is needed, which contains a queen bee and a certain number of worker bees, liquid food a for ensuring the health of the bee colony, and normal pollen b; the beehive is placed in a culture box for circadian rhythm domestication, and the environment of the bee colony is maintained; after ten days of domestication, a special paper box for pollination is loaded, the number of worker bees in the bee colony is 60-80, the bee strength is determined according to the area of the greenhouse, and the bee colony is moved to a buffer room with a temperature of 20 DEG C for pretreatment for 2-3 days before being placed in the greenhouse for pollination; after one week, the pollination efficiency is recorded according to the bite mark rate on the flowers of tomatoes, and the feed in the box is supplemented in time during the pollination period; on the basis of the original ground bee feeding condition, the feeding time is regulated by adding certain light and temperature, so that the pollination efficiency of the ground bees is greatly improved, and the value of the ground bees for better serving the greenhouse facility agriculture pollination is improved.

The invention discloses a method for producing mature honey by traditional breeding of apis cerana, which adopts a multi-layer grid combined beehive and utilizes the habits that internal bees in a bee colony store honey upwards and worker bees can fully absorb honey sacs when being stimulated. In the evening after bees collect honey and return to the nest on the same day, after no nectar enters the nest from the outside, honey combs to be harvested in the beehive are placed at the lowest part of a bee colony. The inner bees transfer the uncovered honey on the honey combs in the beehive to the upper part of the honeycomb at night, and the beehive is taken out before the collection bees of the bee colony collect new nectar on the next day; the taken-down beehive is placed at a hive entrance of a bee colony, the beehive is lightly knocked for 90-200 seconds, bees in the beehive can find food which is easy to absorb to fill the honey bags due to stress reaction, after 90-200 seconds, the bees on the honey combs are repelled away by means of strong vibration or smoke or air blowing and the like, and then the fully-sealed honey combs can be obtained. And cutting off the fully-sealed honey comb to obtain mature honey produced by traditional feeding.

This invention relates to the field of biotechnology, specifically to a PiRNA primer set for detecting *Gnaphalium affine* infection in Italian honeybees, a kit for the same, and its application. The primer set includes the following primers: piR-1182394-F, piR-1182394-R, U6-F, and U6-R. The nucleotide sequence of piR-1182394-F is shown in SEQ ID No. 1; the nucleotide sequence of piR-1182394-R is shown in SEQ ID No. 2; the nucleotide sequence of U6-F is shown in SEQ ID No. 3; and the nucleotide sequence of U6-F is shown in SEQ ID No. 4. This study discovered and identified a specific PiRNA molecule, piR-1182394, whose expression changed significantly after *Gnaphalium affine* infection of Italian honeybee worker larvae. The primer set provided in this study can be used for the early diagnosis of chalkbrood in Italian honeybees, with a diagnostic time earlier than traditional methods. It can provide a diagnostic basis for whether bee colony larvae are infected with chalkbrood before the larvae develop symptoms, providing a new perspective and target for elucidating the molecular mechanism of Italian honeybee resistance to chalkbrood, and laying a theoretical foundation for the future development of novel bee disease control technologies based on RNA interference.

The invention relates to a convenient bee pupa collecting device, which belongs to the technical field of wasp breeding, and comprises a beehive, at least one frame access opening is formed in the beehive, a honeycomb frame is arranged in the frame access opening, a honeycomb frame outer baffle and a honeycomb frame inner baffle are respectively arranged at the front end and the rear end of the honeycomb frame, and a guide rail corresponding to the honeycomb frame in position is arranged on the inner wall of the beehive. The honeycomb frame is arranged on the guide rail, a honeycomb is arranged in the honeycomb frame, the sizes of the honeycomb frame outer baffle and the honeycomb frame inner baffle are both larger than the honeycomb frame inlet and outlet, and the gap between the honeycomb frame and the honeycomb frame inlet and outlet is smaller than the size of worker bees. By taking out the bee pupae through the device, the bee pupae can be rapidly and efficiently taken out, worker bees can be effectively prevented from hurting people, and therefore the efficiency and safety of collecting the bee pupae are greatly improved.

The utility model provides a cell base strip and a cell base strip fixing structure, the cell base strip can be easily taken down from a pulp frame and can improve productivity, the cell base strip comprises a main body strip, the main body strip is provided with rows of holes, and two ends of the main body strip are provided with hanging lugs used for being hung on the pulp frame; holes are additionally formed in the two ends of the rows of holes in the main body strip, so that the distance between the positions, closest to the end edge of the main body strip, of the holes in the two ends of the rows of holes and the end edge of the main body strip is smaller than the outer diameter of the holes. The number of holes in the cell base strip can be increased, under the conditions that the length of the cell base strip is not increased and a conventional beehive standard is still adapted, royal jelly production places in the beehive are increased, according to the fact that one cell base strip is provided with 32 holes in the past, after one hole is added to each of the two ends, the royal jelly production places are increased by 6.7%, the royal jelly production capacity is improved, and the royal jelly production efficiency is improved. The length of gaps on the two sides of the cell base strip is shortened, the area, where beeswax can be made by worker bees, of the two ends is reduced, the cell base strip can be taken down from the pulp frame more easily compared with an existing cell base strip, and the limiting effect is good.

The invention discloses a rapid detection method for family composition of a bee colony, and belongs to the technical field of genetic breeding of bees. The method comprises the following steps: randomly collecting a plurality of worker bee individuals of a to-be-detected bee colony, respectively intercepting the right posterior foot tissue of each worker bee, mixing and grinding, and extracting total DNA to obtain a unified colony DNA sample; carrying out whole genome sequencing on the sample, comparing a sequencing read segment to a csd gene region, and calculating the frequency of each allele; alleles of queen bees and drone sources are distinguished according to frequency distribution, and the composition proportion of each paternal family is calculated based on the frequency of the alleles of the drone sources. According to the method, the right posterior foot tissue is intercepted in a standardized manner, so that the relative constancy of the individual DNA amount in the mixed sample is ensured, and the quantization deviation is eliminated; and in combination with a high-throughput sequencing strategy, the complex family structure of the bee colony and the potential inbreeding risk can be quickly and accurately analyzed at low cost only by one-time sequencing.

The invention belongs to the technical field of bee breeding, and particularly relates to application of Bombella intestini in improving the 10-HDA content of royal jelly. According to the invention, it is found that the ability of worker bees to synthesize 10-HDA can be significantly improved by exogenously supplementing Bombella intestini with a specific concentration. Meanwhile, the method is safe, efficient and sustainable, does not influence bee colony health and bee product safety, can be used in combination with a traditional nutrition regulation and control method, meets the requirement of the market for high-quality royal jelly, and has broad market prospects. And a feasible technical support and a solution are provided for realizing healthy breeding of bees, standardized production of royal jelly and development of green organic bee products.

The invention discloses a BIM-based building structure intelligent scanning monitoring device and method, and belongs to the technical field of building structure monitoring. The invention discloses a BIM-based intelligent scanning and monitoring device for a building structure. The BIM-based intelligent scanning and monitoring device comprises an automatic marking and laying module, an unmanned aerial vehicle scanning module and a central control module, the automatic mark laying module adopts a multi-machine cooperative bee colony operation mode and comprises at least two worker bee units responsible for low areas and at least one drone unit with a lifting stand column. Through a multi-mode marking system combining physical marking and virtual optical marking, various complex wall surface conditions such as glass curtain walls, rough concrete and smooth ceramic tiles are intelligently adapted, the problems of scanning data splicing failure and precision reduction caused by feature deficiency are fundamentally solved, a multi-machine cooperative bee colony operation mode is adopted, and the working efficiency is improved. And parallel operation of high and low airspaces and different areas is realized.

The invention relates to the technical field of biology, in particular to a circRNA primer group for detecting apis cerana infected by bee glomus as well as a kit and application of the circRNA primer group, and the primer group comprises the following primers: ac2651-F, ac2651-R, actin-F and actin-R; the nucleotide sequence of the ac2651-F is as shown in SEQ ID No. 1; the nucleotide sequence of the ac2651-R is as shown in SEQ ID No. 2; the nucleotide sequence of the actin-F is as shown in SEQ ID No. 3; and the nucleotide sequence of the actin-R is as shown in SEQ ID No. 4. The research finds and identifies a specific circRNA (Ribonucleic Acid) molecule ac2651 of which the expression is obviously changed after worker bee larvae of apis cerana are infected by bee glomus, the primer group provided by the research can be used for early diagnosis of the apis cerana chalkbrood disease, the diagnosis time is earlier than that of a traditional method, a diagnosis basis can be provided for whether bee colony larvae are infected by chalkbrood disease or not before the larvae are attacked, and the method has the advantages that the method is simple and convenient to operate and high in practicability. A new view angle and a new target spot are provided for clarification of a molecular mechanism of Chinese bee chalkbrood disease resistance, and a theoretical foundation is laid for development of a novel bee disease prevention and control technology based on RNA interference in the future.

The utility model relates to a special box for spawning of bred bumblebees. The special box comprises a box body, a breeding box arranged in the box body, a feeding box arranged in the box body, a first plate for sealing the breeding box, a second plate for sealing the feeding box, a first grating facilitating entering and exiting of drones and worker bees and a second grating preventing queen bees from flying out. The first plate is connected to the breeding box in a sliding manner; the second plate is rotationally hinged to the feeding box; the first grating is arranged between the box body and the feeding box; the first grid is arranged between the breeding box and the feeding box. The problems that after the breeding cavity is opened, the breeding cavity is opened, and then the queen bee easily escapes are solved, and when the queen bee needs to be caught, whether the queen bee escapes from the gap between the cover plate and the box body or not needs to be noticed when an ordinary box cover is opened, and the queen bee escapes are solved.

The application relates to a SNP marker for identifying the royal jelly high-yield trait of an Apis mellifera colony and application thereof. The SNP marker is selected from NC_037641.1_10806386 and NC_037641.1_10806436. Adult worker bee individuals are randomly collected from an Apis mellifera colony to be detected, and genomic DNA is extracted as a template for a PCR amplification reaction to obtain a PCR reaction product; the obtained PCR reaction product is detected by agarose gel electrophoresis, and the PCR reaction liquid with a target band is sequenced; the sequencing result is analyzed, the gene frequency of the NC_037641.1_10806386 site or the NC_037641.1_10806436 site of the Apis mellifera colony to be detected is calculated, and whether the Apis mellifera colony to be detected is a royal jelly high-yield colony is determined. The application provides two SNP markers related to the royal jelly high-yield trait of the Apis mellifera from the molecular biology level, the two SNP markers are contained in a target fragment amplified by a same pair of specific primers, the royal jelly high-yield trait of the Apis mellifera colony can be scientifically, accurately and quickly identified, and the breeding cycle of the royal jelly high-yield Apis mellifera can be greatly shortened, and the breeding speed can be accelerated.

The utility model relates to a queen bee oviposition controller suitable for multiple box types, and belongs to the technical field of bee breeding. One end of the telescopic frame is telescopically inserted into the outer frame, the outer frame and the telescopic frame form a frame with an opening in the top, mesh holes allowing worker bees to pass through are evenly formed in the two sides of the outer frame and the two sides of the telescopic frame, supporting holes allowing frame lugs of the honeycomb frame to stretch out are formed in the outer end of the outer frame and the outer end of the telescopic frame, and the top of the telescopic frame is covered with the first cover plate. The second cover plate covers the top of the outer frame, and the outer frame, the telescopic frame, the first cover plate and the second cover plate form a space for restraining a queen bee; the honeycombs are placed in the frame, the queen bees are placed in the space for restraining the queen bees, worker bees can freely enter and exit through the meshes, and the queen bees can be limited to lay eggs on the honeycombs so as to obtain eggs of the same day age; the outer frame and the telescopic frame are installed in a telescopic mode, the size of the space for restraining the queen bee can be adjusted according to the honeycomb size of the beehive of the corresponding model, the device can adapt to beehives of various models, universality is achieved, and applicability is improved.

The application relates to the field of insect behavior, and particularly discloses a method for monitoring queen bee movement, diurnal rhythm and sleep of Apis mellifera, which comprises the following steps: S1, preparation; S2, 5mL of 50% sugar water is filled into a rubber head dropper a, the rubber head dropper a is fixed on a worker bee monitoring tube c, then a steel wire mesh b is covered on the non-sugar water end, and finally the aforementioned components are connected and fixed with a queen bee monitoring tube d; S3, the worker bee and the queen bee are put into the queen bee monitoring device, then the queen bee monitoring device is put into an insect behavior monitor LAM25, a computer is connected for monitoring; and S4, data analysis; on the basis of the original insect behavior monitoring system, the research object is specially the Apis mellifera species, and the monitoring device is optimized according to the monitoring data; the queen bee monitoring device solves the problem of excessively low survival rate of the queen bee in the monitoring, the healthy queen bee guarantees the accuracy of the data, and a good behavior basis is provided for queen bee breeding.