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A method for identifying high-yield traits of royal jelly in bee colonies using the snp marker rs4208349

A bee colony royal jelly, high-yield technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of high cost, complex identification process, unfavorable popularization, etc., to improve the success rate, rapid identification, and efficiency. Effect

Inactive Publication Date: 2018-02-09
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

The more reliable ones are the three molecular markers screened by Pan Jiao et al. (2012) using gene chip technology within the range of the honeybee genome. However, this method requires the extraction of honeybee RNA and the use of fluorescent quantitative instruments, which makes the identification process complicated and expensive. , which is not conducive to the popularization of the method

Method used

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  • A method for identifying high-yield traits of royal jelly in bee colonies using the snp marker rs4208349
  • A method for identifying high-yield traits of royal jelly in bee colonies using the snp marker rs4208349
  • A method for identifying high-yield traits of royal jelly in bee colonies using the snp marker rs4208349

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Embodiment 1

[0040] A method utilizing SNP marker rs4208349 to identify high-yield traits of honey bee colony royal jelly, comprising the following steps:

[0041] (1) Sampling of bees: 50 adult worker bee individuals were collected from the bee species with high royal jelly production ("Zhenongda No. 1") and the bee species with low royal jelly production (Apis mellifera, USA), and each individual was placed in a centrifuge tube. Freeze at -20°C for later use;

[0042] (2) Extraction of DNA from bee samples (using Sangon’s Animal Genome Rapid Extraction Kit, SK8222)

[0043] Use ophthalmic scissors to cut off the limbs, wings and abdomen of the individual worker bees in step (1), keep only the head and thorax, put them in a mortar filled with liquid nitrogen, crush them with a grinding rod, and then transfer them to a 1.5 mL centrifuge tube Add 400 μL Buffer Digestion, shake evenly, and place in a water bath at 65 °C for 1 h until the cells are completely lysed. Then remove the supern...

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Abstract

The invention relates to a method for identification of bee colony royal jelly high yield characters by SNP (single nucleotide polymorphism) marker rs4208349. The method includes: bee individual sampling, bees sample DNA extraction, primer synthesis, PCR detection and identification of bee colony royal jelly high yield characters. For the bee individuals randomly collected from the bee colony, according to whether significant difference exists between the occurrence frequency PA of A allele at the SNP marker rs4208349 and the occurrence frequency PG of G allele, the bee colony royal jelly high yield characters can be identified. From the molecular biological level, the invention provides the method using the apis mellifera worker bee individual royal jelly high yield related SNP (rs4208349) marker to scientifically, accurately and rapidly identify the apis mellifera colony royal jelly high yield characters, can greatly shorten the breeding cycle of high royal jelly producing apis mellifera species, and accelerates the bee breeding speed.

Description

technical field [0001] The invention relates to a method for identifying high-yield traits of bee colony royal jelly, in particular to a method for identifying high-yield traits of bee colony royal jelly by using SNP markers, and belongs to the field of biotechnology. Background technique [0002] Royal jelly has many important nutritional and medicinal values, is very beneficial to human health, and is a natural nourishing food. my country is a big beekeeping country, and the output of royal jelly accounts for more than 90% of the world's total, which has an inseparable relationship with the bee species raised in our country. Our country's bee industry scientists and scientific and technical personnel have bred "Zhenongda No. 1" and Xiaoshan jellyfish through the closed breeding method of bee groups. , Has become my country's unique and main bee species. For a long time, my country's bee industry scientific and technological workers have been committed to the research of h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 苏松坤刘小彦李志国刘元珍孟魏宏
Owner FUJIAN AGRI & FORESTRY UNIV
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