12 results about "Chinese bee" patented technology

The utility model discloses an auxiliary device for breeding apis cerana in a soil slope or a ridge, which is characterized by comprising a box-shaped main body (1), a front door (2) of the box-shaped main body, an exhaust pipe external connecting pipe (3), an air inlet and water outlet pipe external connecting pipe (3) and a nest door external connecting device. When the auxiliary device is used, a soil hole with a proper size is dug in a soil slope or a ridge, the box-shaped main body of the auxiliary device is put in the soil hole, a beehive is put in the box-shaped main body, a beehive door external connection device is installed, and then a front door, an exhaust pipe external connection pipe and an air inlet drainage pipe external connection pipe of the box-shaped main body are installed. The beehive placed in the auxiliary device can obtain more excellent in-hole production conditions through the auxiliary device, and healthy, safe and efficient production of Chinese bees is guaranteed.

The utility model discloses an apis cerana feeding device, which relates to the field of apis cerana feeding devices and comprises a food storage box used for storing sweet water, pollen or other bee food. The feeding trough is used for containing food for bees to eat, and the bottom of the food storage box is provided with a food feeding pipe communicating with the upper portion of the feeding trough; the automatic control module comprises a quantity control electromagnetic valve, the quantity control electromagnetic valve is arranged on the feeding pipe, and when the quantity control electromagnetic valve is opened, bee food stored in the food storage box can be fed into the feeding trough through the feeding pipe. The automatic control module can automatically adjust the feeding amount according to the activity condition of bees and the environmental condition, it is ensured that each bee can obtain enough food, and the feeding efficiency is improved; the frequency of manually putting food is reduced, the stress response of bees is reduced, and the health level and productivity of bee colonies are improved; the double-layer heat preservation storage box and the automatic control module ensure that food is fresh and safe, and bee colony diseases caused by food deterioration are reduced.

The invention discloses an application of a heat-resistant related gene Hsc70-4 of apis cerana and an encoding protein of the heat-resistant related gene Hsc70-4, and belongs to the technical field of cytobiology and molecular biology. The invention verifies that silent Hsc70-4 can reduce the survival rate of Chinese bees under heat stress, and overexpressed Hsc70-4 can increase the survival rate of drosophila S2 cells under heat stress. Induced expression of Hsc70-4 is helpful for reducing oxidative damage of heat stress to apis cerana and improving heat resistance of apis cerana. In view of the heat resistance of the Hsc70-4, overexpression of the Hsc70-4 in bees or economic animals is expected to be realized through a transgenic technology in the future, so that the heat resistance of the Hsc70-4 is improved, the yield of the Hsc70-4 under a heat stress condition is further improved, and a new gene target is provided for coping with ecological and agricultural challenges under a global warming background.

The invention relates to the technical field of gene detection, in particular to a primer group for detecting a beta-GBP gene of apis cerana and application of the primer group, and the primer group comprises an upstream primer CDS-Acbeta-GBP-1-F with a nucleotide sequence as shown in SEQ ID No.1; the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-1-R is as shown in SEQ ID No. 2; the nucleotide sequence of the upstream primer CDS-Acbeta-GBP-2 is as shown in SEQ ID No.3, and the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-2-R is as shown in SEQ ID No.4; the nucleotide sequence of the upstream primer CDS-Acbeta-GBP-3-F is as shown in SEQ ID No.5, and the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-3-R is as shown in SEQ ID No.6. The invention further discloses a kit for detecting the content of the CDS-Acbeta-GBP-3-F. The Ac beta-GBP gene found by the research can be used as a molecular breeding target spot, disease-resistant bee species highly expressing the gene can be screened by detecting the Ac beta-GBP gene through the primer pair provided by the invention, the method for screening the chalkbrood disease-resistant bee species is enriched, and meanwhile, the Ac beta-GBP provides a new view angle and a new target spot for illuminating a chalkbrood disease-resistant molecular mechanism of Chinese bees, so that the application prospect is wide. And a theoretical basis is laid for developing a novel bee disease prevention and control technology based on RNA interference.

The invention discloses an apis cerana DnaJC28 gene as well as an encoding protein and application thereof, and belongs to the technical field of cytobiology and molecular biology. The invention finds that apis cerana DnaJC28 is positioned in cytoplasm through research for the first time. The short-term heat stress and the long-term heat stress can induce the expression of the apis cerana DnaJC28, the silent DnaJC28 can reduce the survival rate of the apis cerana under the heat stress, and the heterologous overexpression apis cerana DnaJC28 can increase the survival rate of the drosophila S2 cells under the heat stress. A plurality of antioxidant genes can partially make up the function of DnaJC28 under heat stress, and oxidative damage caused by heat stress to Chinese bees is reduced. The gene can be used as a novel heat-resistant gene target, the gene is over-expressed in other economic animals in the future, the yield and the heat resistance of the gene under the heat stress condition are expected to be improved, and a sustainable solution is provided for livestock production under the climate change.

The method comprises the following steps: screening virgin queen bees and drones of Chinese bees naturally bred in a southwest origin area, and domesticated first target drones and second target drones, and establishing a hybrid combination; taking the first target drone as a male parent, hybridizing with the native Chinese bee virgin queen bee as a female parent to obtain a first target hybrid filial generation, and taking the second target drone as a male parent, hybridizing with the native Chinese bee virgin queen bee as a female parent to obtain a second target hybrid filial generation; backcrossing male apis cerana in the origin place as a male parent and excellent virgin queen bees of the hybrid filial generations of the first target and the second target as female parents respectively to obtain first and second backcross filial generations respectively; respectively carrying out composite hybridization on the first target and second target backcross offspring excellent drones and excellent virgin queen bees to respectively obtain first and second composite cross offspring Chinese bees; through multi-generation multi-cross breeding, excellent swarms with high reproduction rate, high honey yield, strong stress resistance and genetic stability are obtained through screening.

The utility model discloses a multifunctional soil breeding frame, and belongs to the technical field of bee breeding. The nest frame comprises a concave upper frame beam, a top cover plate, a left frame, a right frame, a lower frame beam, sliding block units, steel sticks and screws, the concave upper frame beam and the top cover plate form a feeder, a concave groove is reserved in the bottom of the feeder, the four frames are connected through mortise and tenon joint structures and are reinforced through the screws, and a plurality of sets of sliding blocks are buckled on the left frame and the right frame. Steel sticks are placed at the notches of the same horizontal sliding block to form a frame with the interior capable of moving flexibly; the beehive is provided with the feeder, the groove for installing the guide foundation is reserved, the sliding block units can be flexibly detached and moved, completeness of remaining honeycombs after honey taking is guaranteed, an environment for new honeycombs to produce birth is created for Chinese bees, operation is easy, the problems that bee growers check bees, change queen bees, swarming bees, frequently open the beehive and add frames, and hurt the birth and the bees after honey taking are solved, and the beehive is suitable for being popularized and used. A plurality of frames can be added at a time, the original ecological habit of ball holding development of bees is truly restored, the cost is effectively saved, and the capacity of bee colonies is improved.

The utility model relates to the technical field of mating boxes, and discloses an apis cerana mating box which solves the problem that frames in an existing mating box are inconvenient to adaptively arrange, and comprises a cover body arranged at the top end of a box body, a plurality of frames arranged in the box body, and a first lap plate fixedly arranged at the top end of one side of each frame. A first lap-joint plate is fixedly arranged on one side of the frame, a first half groove is formed between the first lap-joint plate and the frame, a second lap-joint plate is fixedly arranged on the other side of the frame, a second half groove is formed between the second lap-joint plate and the frame, a first threaded hole is formed in the middle of the top end of the frame, second threaded holes are formed in the first lap-joint plate and the second lap-joint plate, and locking bolts are arranged on the second threaded holes. A rectangular supporting frame body matched with the frame is fixedly arranged at the top end of the inner side wall of the box body; by means of the mating box, a single frame can be longitudinally arranged or two frames can be spliced to be transversely arranged according to needs, and adaptability is improved.

The utility model discloses a horizontal Chinese bee hive for bee breeding, which relates to the field of bee breeding and comprises a honeycomb for providing a place for inhabiting and breeding a bee colony; the beehive is mounted outside the honeycomb and used for protecting the honeycomb inside the beehive; the beehive comprises two symmetrically-arranged heat preservation plates which are installed on the inner wall of the beehive in a sliding mode. The two groups of frames are symmetrically arranged and are mounted in the beehive; the vertical partition plates are fixedly connected with clamping grooves in the inner wall of the beehive; each of the two groups of symmetrically arranged frames comprises a frame transverse hanger and a frame transverse hanger; the frame transverse hangers are connected with the inner wall of the beehive in a sliding manner; the honeycomb is fixedly mounted at the bottom of the frame transverse hanger; according to the horizontal Chinese bee hive for bee breeding, through the design of flexible disassembly, a bee farmer can conveniently replace damaged parts of the hive, and through the movable arrangement of the honeycomb, the bee farmer can adjust the number and position of the honeycomb frames according to needs.

The application discloses a method for breeding strong colonies of honeybee larvae with high efficiency, and belongs to the technical field of honeybee breeding. The method comprises the following steps: after overwintering, two colonies are put into one beehive, and the colonies are separated by a queen separator for outdoor spring breeding; during the outdoor spring breeding, the queenless larvae in the colonies are taken out, put into a beehive with a feeding device at the bottom for indoor incubation, and a queenless feeding colony is formed; after the queenless feeding colony is completely covered, the queenless feeding colony is moved out of the room, and is gradually added into the outdoor breeding colony, so that the queenless feeding colony is hatched out of the room; the indoor incubation step is repeated until the external temperature is stable, and honey powder sources appear. The method has obvious effects on breeding strong colonies and increasing production, has important significance for improving the economic benefits of the beekeeping industry, and provides a guarantee for the development of the Chinese bee industry.

The invention belongs to the technical field of honey detection, and particularly relates to a method for identifying Chinese mountain sapium sebiferum honey and Italian mountain sapium sebiferum honey based on the amino acid ratio. The method for identifying the Chinese alpine mountain sapium sebiferum honey and the Chinese alpine mountain sapium sebiferum honey provided by the invention comprises the step of identifying the Chinese alpine mountain sapium sebiferum honey and the Chinese alpine mountain sapium sebiferum honey on the basis of the ratio of the proline content in the total content of free amino acids and / or the ratio of the phenylalanine content in the total content of the free amino acids in a honey sample. The method has the advantages of simplicity and convenience in operation, rapidness, high accuracy and the like, can realize rapid identification of the source of the Sapium discolor honey species, provides technical support for authenticity identification of honey, and has important significance in promoting healthy development of the honey industry.