150 results about "Chinese bee" patented technology

The invention discloses a conveniently cleaned Chinese bee hive which comprises a bottom box. A box cover is arranged on the upper portion of the bottom box, more than two honeycombs are arranged in the bottom box, the space in the bottom box is divided into two symmetric breeding chambers by a first vertical queen excluder, a drawable bearing plate is arranged on a bottom plate of the bottom box, a baffle plate is vertically fixed at a draw-out end of the bearing plate, honeycomb doors are arranged on two sides of the baffle plate respectively, and the baffle plate is hermetically fastened with the front end face of the bottom box when the bearing plate is not drawn out of the bottom box. By the aid of the drawable bearing plate arranged on the bottom plate of the bottom box, broken combs, wax-moth larvae and impurities in the bottom box can be conveniently cleaned and arranged, and the inside of the box is kept clean and neat. Besides, a partition plate and / or a second vertical queen excluder are arranged on two sides of the inside of the bottom box respectively, and a honey storage area and a breeding area are respectively meticulously managed. Honey is collected from the honeycombs in the honey storage area without opening the breeding area, so that larvae and bee pupae cannot be injured.

The invention provides an original ecological beekeeping method and an original ecological beehive thereof which can provide honey for a plurality of times. The invention employs the original ecological beehive with movable covers on the top and the bottom and supporting strips at the middle part. Beekeeping and honey obtaining steps are that the following: 1. the bees are led to spit wax and make comb in the beehive in the state of standing stably; a honey storing area is arranged on the top of the comb, at the bottom of which is a breeding area; when the honey storing area is full, the covers are closed; then the honey is cut down and collected from the honey storing area, while the cutting operation can not exceed the supporting strips in the middle part; 2. after honey collection is finished, the body of the beehive is turned upside down; currently, the breeding area is at the upper part; after larva bee are out of the beehive, the breeding area is changed into a honey storing area which can store honey continuously; while the lower part is the cut original honey storing area, on which the bee can continuously spit wax and make comb to form a new breeding area; when the newly formed honey storing area is full and covered by the covers, the step 1 is repeated and the honey storing area is cut down to collect honey; 3. the step 2 is repeated.

The invention discloses a method for breeding a high-quality mite-resistant bee species. The method is characterized by comprising the following steps of: (1) selecting breeding materials: screening a 'Jiangshan No.2' Italian bee with a high royal jelly yield, a French pure-bred Italian bee with excellent honey making performance and a healthy local Chinese bee species with excellent mite resistance for serving as an introduction object according to series indexes such as mite resistance, productivity, vitality and the like; (2) breeding a mite-resistant bee species: breeding a breeding male bee from Jiangshan No.2, breeding a Jiangshan No.2 virgin queen bee according to the requirements of bee biology and genetic breeding characteristic, and performing artificial fertilization hybridization with the bred breeding male bee to form a Jiangshan No.2 bee species with high production performance and disease resistance for serving as a male parent, wherein a pure-bred Italian bee with excellent honey making performance introduced from France serves as a female parent; and (3) checking and cutting a French Italian bee drone pupae every 7 days from breeding in spring till the end of breeding. The harm of bee mites to bee colonies can be avoided for beekeepers, the reproduction speed of the bee colonies is increased, and the yields and qualities of bee products are improved.

The invention discloses a bee breeding method which includes the following steps that 1, a nectar source field is selected; 2, a beehive is arranged, a partition plate divides the beehive into an upper region and a lower region, and honeycombs are arranged in both regions; 3, queen bee breeding is conducted, the two regions partitioned by the partition plate and a super are each internally provided with a Chinese bee queen and a West bee queen, mixed feed composed of pollen, amino acid and bean protein powder is added to the Chinese bee queens and the West bee queens in the spawning period, honey water is fed, after the Chinese bee queens and the West bee queens lay eggs, honey and fresh ginger water are injected into the honeycombs to feed the bee queens; 4, as the temperature drops, the eggs laid by the bee queens are reduced, the partition plate is opened, the West bee queen is transferred, West bees are involved into Chinese bees, and the honey and the fresh ginger water are injected into the honeycombs to feed worker bees and the bee queens. According to the bee breeding method, Chinese bees are mainly bred, west bees are added at proper time so as to reduce plant diseases and insect pests generated when the Chinese bees are bred, and the problems that it is difficult for the Chinese bees to survive the winter, and honey is not easy to take are solved.

The invention relates to a breeding method of queen bees. The method comprises the specific steps of selection of a field, an environment, selection of a bee species, selection and demands of a queenbreeding colony, organization of the queen breeding colony, management of the queen breeding colony, larval transplantation for queen breeding, larval transplantation, organization of a coupling colony, a queen introduction method, a management method of the coupling colony, coupling control over Chinese bees, queen replacement time of the Chinese bees and a queen replacement method of the Chinesebees. By means of the method, a proper environment and rich nutrient conditions are created, according to the number and time which are required by a plan, batched good-quality queen bees are bred, and accordingly the aims of colony multiplication and good species popularization are achieved. The sexual maturity periods of drones and the queen bees are reasonably selected, and the sexual maturityperiod of the bred queen bees can be matched with the puberty of the drones. During queen breeding, a colony which is poor in swarming performance and good in species character and can maintain the large swarm is reasonably selected, used as a breeding colony and used for breeding new queen bees and drones respectively. By reasonably using queen replacement of the queen bees and factors such as the climate, the honey source, the population of the bee colony and the behavior and physiological states of the queen bees, introduction of the queen bees easily succeeds.

The invention provides a honeybee breeding method and belongs to the technical field of animal husbandry. The breeding method includes the following steps: firstly, select a bee breeding place, set up a beehive, and regularly apply acaricide to the beehive to remove bee mites; then, select North China bee, Italian bee and Northeast black bee as parents, Breeding bees with stable and excellent properties as bee species; and feeding feed a made of honey, white sugar, orange juice, tomato juice, longan juice, grape juice, citric acid, edible salt, water, and natural pollen, Feed b made of defatted soybean flour, white sugar and appropriate amount of water. The invention provides a honeybee breeding method. The breeding method is safe and reliable. The North China honeybee, Italian honeybee and Northeast honeybee are scientifically bred to obtain a honeybee with high yield, cold resistance, mite resistance and disease prevention. species, and then fed scientifically and rationally to promote the healthy development of bees, reduce the incidence of morbidity, and improve the quality and food safety of honey.

The invention relates to the technical field of bee feeding, in particular to a Chinese bee feeding device with possibility of traditional feeding and movable-frame nectar fetching for many times, and a keeping method. The Chinese bee movable-frame feeding device comprises a movable hive bottom, a hive, a super and a movable hive cover, wherein a movable bee space frame is further arranged between the super and the hive and can be convenient to take out to allow a hive body structure to be capable of being changed; when the movable bee space frame is taken down, no up-and-down bee space exists between the hive and the super; when the movable bee space frame is mounted, an up-and-down bee space exists between the hive and the super; during bee keeping, when the nectar secreting amount of an outside nectar source is small, the movable bee space frame is not added after nectar is fetched, the beehive is turned over up and down to allow bees to freely build a comb and give birth downwards, and an old comb at the upper part becomes a nectar storage area; when the nectar secreting amount of the outside nectar source is large, the movable bee space frame is added, the beehive is not turned over, the upper part is used for production, and the lower part is used for reproduction; the bee reproduction is unaffected; bee diseases are few; a bee colony develops quickly; the nectar yield is high; the nectar fetching for many times can be realized simply; and the bee reproduction is unaffected.

The invention provides a Chinese bee bottle type hive honey flat box production method and device and a frame for production. The Chinese bee bottle type hive honey flat box production method includes steps of selecting a manual septum made of beewax to be in a honey bottle; partitioning a honeycomb having a honey storage area, a powder storage area and a raising area along the boundary of the honey storage area and the powder storage area according to habits of Chinese bees, removing the honey storage area, reserving a lower honeycomb with the powder storage area and the raising area, fixing the lower honeycomb within a small frame having equal height; partitioning a beehive into two parts, forming a bottom hive comprising the bottom and a four-wall frame cover; arranging the small frame in the bottom hive; arranging a partition on the upper surface of the bottom hive, disposing a honey bottle with a bottle mouth downwards on a partition so that bees can produce hive honey in a new honey storage area formed in the honey bottle. Bottled hive honey can be produced by flat boxes, honey super is not needed to be added, labor intensity is reduced, and the Chinese bee bottle type hive honey is applicable to bee species easy to hive off or small in the colony such as Chinese bees.

The invention discloses a Chinese bee fast queen separating method. Beehives are used for queen separation, each beehive in a square shape, edge plates and longitudinal plates are arranged inside each beehive, when brood combs in the beehives reach 4 to 5 frames, queen separation plates are used for clamping in the longitudinal plates for averagely separating the beehives into two spaces, 2 to 3 beehive frames are respectively placed onto the edge plate of the two places, the brood combs, honey combs and bees are averagely distributed, the original queen bees are placed at one side, one newly ovigerous queen bee is placed at the other side, at the moment, collecting bees fly into a left space and a right space of the beehives when returning to the beehives, and the number of the beehive frames can be regulated if the condition that the bees in one beehive are few, so that the bee number is averaged. When the Chinese bee fast queen separating method is adopted, the investment and special management measures do not need to be added, the original group can be developed into strong production groups, and in addition, the separated bee groups can also become strong production groups, so the goals of increasing the bee group number, enlarging the production capability and improving the bee product capability are achieved.

The invention discloses a method for breeding a high-quality anti-mite bee variety. The method is characterized by comprising the following steps of: 1) selecting breeding materials, wherein 'jiangshan No.2' Italian bees with relatively high royal jelly yield, France pure Italian bees with high honey production performance and healthy local Chinese bees with high anti-mite performance are selected as species introduction objects according to a series of indexes such as anti-mite performance, productivity and vitality; 2) breeding the anti-mite bee variety, wherein breeding drones are selected from the jiangshan No.2, selecting a bred jiangshan No.2 virgin queen bee according to the requirements of honey bee biology and inheritance breeding characteristics, and performing the artificial fertilization and cross breeding between the breeding drones and the bred jiangshan No.2 virgin queen bee so as to obtain a jiangshan No.2 bee variety with high production performance and disease resistance, using the jiangshan No.2 bee variety as male parents and using the pure Italian bees which are introduced from France and have high honey production performance as female parents; and 3) since breeding starts in spring, detecting and cutting off pupae of the France Italian bees every other 7 days till the breeding ends. The method can solve the problem that bee colonies are jeopardized by bee mites for beekeepers and improve breeding speed of the bee colonies and the yield and the quality of bee products.

The invention discloses a preparing method and application of an odorant binding protein sensor based on impedance analysis. A plane interdigital gold electrode array is formed by machining in a standard MEMS technology, a nitrocellulose membrane is used for processing the surface of an interdigital gold electrode, odorant binding protein is directly fixed, and the impedance sensor is obtained. An analyzing method with impedance and molecular docking combined is used, and the Chinese bee odorant binding protein Acer-ASP2 is fixed on the interdigital gold electrode and is used for detecting flower fragrance matter and bee pheromone. The established electrochemical sensor can fix the odorant binding protein on the surface of the interdigital gold electrode stably and easily, sensor detecting sensitivity is high, detecting lower limit is low, and specificity is strong.

The invention discloses Chinese bee feed and a preparation method thereof. The Chinese bee feed is prepared from the following components: 45-55% of bee pollen, 13-15% of honey, 10-15% of white sugar, 10-15% of protein powder, 3-7% of dried figs, 3.5-4.5% of raisins, 0.5-1% of citric acid, 0.1-0.2% of salt and 0.3-0.4% of riboflavin. The Chinese bee feed is prepared by the following steps: a, preparing the raw materials: grinding the dried figs, the raisins, the honey, the white sugar, the protein powder, the salt and the riboflavin respectively and sieving; and b, treating the bee pollen: immersing the bee pollen with water and putting the mixture into a steamer to be steamed; c, mixing and boiling: mixing the honey, the white sugar, the protein powder, the salt, the riboflavin and the dried fig dry powder with the raisin dry powder and then boiling syrup, and mixing the citric acid and the bee pollen treated in the step b into the syrup; and cooling to obtain the product. The Chinese bee feed disclosed by the invention has the characteristics that the crowd fertility of Chinese bees can be improved, the crowd is enhanced and the morbidity is reduced, and the method is suitable for popularization and application.

The invention provides a use method of a natural swarming gate plate in a hive of Chinese bees. By means of the use method, natural swarming of the Chinese bees can be achieved in the hive. The use method comprises the steps that firstly, the gate plate is installed in the middle of the hive; secondly, hive door hole queen excluders are installed on hive door holes of the hive; thirdly, bee colonies are placed in a first chamber with the face provided with queue bee exit holes, and hive frames with hive foundations are placed in the first chamber; fourthly, checking is carried out, the queen excluders on the virgin queen colony hive door holes are removed, and combs in an original colony are disposed or destroyed; fifthly, one bee colony is moved out. The use method is easy to implement, learn and understand, saves troubles and labor, greatly reduces the workload of manual breeding of the Chinese bees, avoids huge losses caused when the Chinese bees fly away from the hive and abscond, and is beneficial for protecting the Chinese bee resources.

The invention relates to a medicament for treating Chinese bee sacbrood and process for preparation, wherein the constituents of the raw material include capsule of weeping forsythia 1 part, baikal skullcap root 0.9-1.1 parts, honeysuckle flower 0.9-1.1 parts, and radix isatidis 0.9-1.1 parts. The preparing process includes grilling liquid medicine with water, concentrating and charging bee honey.

The invention provides a beehive for Chinese bees and a method for pollinating greenhouse crops by the Chinese bees. The beehive is provided with a first bottom box (1), a second bottom box (2), a super (3), a super cover (4), a yarn cover (5), a queen-bee separation plate (14), a feeding box (15), nest frames (16) and a nest door board (6), wherein the first bottom box (1) and the second bottom box (2) are detachably connected with each other; the super (3) is detachably connected with the bottom box; the yarn cover (5) is positioned between the super (3) and the super cover (4); the queen-bee separation plate (14) is positioned between the bottom box and the super (3); the feeding box (15) is arranged at the lower part of the bottom box; the frames (16) are respectively arranged in inner cavities of the bottom box and the super (3); the nest door board (6) is arranged at one side of the feeding box (15); the nest door board (6) is provided with ventilation holes (9) and a bee inlet and outlet. The method for pollinating the greenhouse crops by the Chinese bees comprises the following steps of: transferring young bees which emerge out of pupae and are aged between10 to 30 days into any bottom box, and transferring the rest colony into the other bottom box; removing the super (3); mounting the yarn cover and a box cover on the bottom box (2), transferring the bottom box carrying the young bees into the greenhouse, and pollinating the greenhouse crops by the young bees.

The invention discloses a double-queen culture method of Chinese bees. The method comprises the steps of selecting a healthy and strong swarm for rearing a queen bee from a series of swarms in the period of May to June before the natural swarming, selecting on one-day larva from each of the rest bee boxes to be cultured for 9 days in a queen rearing box, locking an old queen in the rest bee boxes and 3 to 6 worker bees onto a boundary board of the bee box, respectively introducing the queen cells into respective bee box after the queen cell is grown, observing cell outing and mating situation of the new queen, wherein if both the cell outing and mating are normal, and no fight exists, the two queens are successfully cultured. By adopting the method, a one-year-old queen and a novel bred queen can be cultured in a same bee box, the fight is avoided, the Chinese bees can be reproduced rapidly, the yield of honey can be increased, and the problems that the economic benefit for culturing the Chinese bees is low and the Chinese bee honey is in shortage in the market can be solved; meanwhile, the Chinese bees can be used for pollinating crops and traditional Chinese herbs, so that the yield of the crops and traditional Chinese herbs can be increased.

The invention relates to a comb honey production box, in particular to a Chinese bee comb honey double-queen production box. The invention provides the Chinese bee comb honey double-queen production box which comprises a comb frame provided with a hollow cavity, wherein a separator plate is arranged in the comb frame, the hollow cavity of the comb frame is separated by the separator plate into a first cavity and a second cavity for containing two queen bees respectively, and combs are respectively arranged in the first cavity and the second cavity. The Chinese bee comb honey double-queen production box has the beneficial effects that the hollow cavity of the comb frame is separated by the separator plate into the first cavity and the second cavity for containing the two queen bees respectively for forming double-queen colonies, in such a way, the colonies are conveniently joined after queen bees are lost, and the colonies are conveniently separated when two queen bees exist, so that stability in the colonies is favorably maintained, the working intensity of peasant households is lowered, and the efficiency of observing the bee colony is favorably improved.

The invention provides a square automatic constant-temperature Chinese bee hive with a sloping bottom, which can discharge waste out naturally, keep a constant internal temperature automatically and separate reproduction from honey storage and honey making. The hive is characterized in that a front bottom plate and a rear bottom plate are fixed beneath a nest box frame; box bottom backing strips with grooves are fixed beneath the bottom plates; in a nest box, a set of automatic thermostat composed of an electronic refrigeration sheet, an electric heating device, a temperature controller and the like is mounted at the rear part of a box bottom, and a sloping bottom plate is mounted on the automatic thermostat, so as to form a nest box with a sloping bottom; and a metal screen plate, a super, an auxiliary cover plate and a box cover are overlapped on the nest box frame in sequence. The hive provided by the invention has the advantages that hazard that wax-moth larvae breed in waste at the hive bottom is avoided, the harm to Chinese bees caused by change of external temperature is overcome, honey is convenient to take, and the quality and yield of Chinese bee honey are improved.

The invention discloses a composition for treating Chinese bee sacbrood and a preparation method thereof and belongs to the technical field of animal epidemic prevention and treatment. The composition is prepared from, by weight, 20-50 parts of Japanese flowering fern rhizomes, 20-50 parts of flos lonicerae, 5-10 parts of radix glycyrrhizae and 400-500 parts of white sugar. The preparation method comprises the following steps that the Japanese flowering fern rhizomes, flos lonicerae and radix glycyrrhizae are put in a container according to respective parts by weight; after 400-500 parts by weight of water is added, the mixture is boiled with a strong fire and then decocted for 15-20 min with a slow fire; the decoction passes through a 10-20 mesh screen, filter residues are discarded, 400-500 parts of white sugar is added into filter liquor and boiled into syrup, and the composition for treating Chinese bee sacbrood can be obtained. The Japanese flowering fern rhizomes, flos lonicerae and radix glycyrrhizae are compounded and then mixed with white sugar which serves as the main material, the prepared composition is sweet in smell and good in palatability, pathogens of sacbrood can be effectively killed, the pathogens are prevented from infecting the respiratory tract, the treatment effect of the composition is maintained, and then the purpose of healing sacbrood is achieved.

The invention relates to a method for cultivating Chinese bee queens in bulk by using Chinese bee natural stylobates. The method is characterized in that the natural stylobates made by Chinese bee swarms are adopted, the natural stylobates and a part which is positioned below 3-5 rows of nests of the root of every natural stylobate are cut off together, and part of honeycombs at the left and right of the stylobate are reserved; the cut natural stylobates are put into a freezing chamber of a refrigerator for cryopreservation, and are taken out for use under the condition that the collected natural stylobates reach batch requirements and requirements of cultivating the Chinese bee queens. According to the method, a special queen-breeding frame for cultivating Chinese bees in bulk is adopted to cultivate the Chinese bee queens, two beams for fixing the stylobate are arranged in the special queen-breeding frame, the taken-out stylobates are adhered to the beams, larvas, being one day old, of the Chinese bees are transferred to the bottom of the adhered natural stylobates by using a larva transferring needle, the special queen-breeding frame is put into a queen cultivating swarm of the Chinese bees for cultivating and is taken out on the day before new queen bees are going to be taken out of a nest, all to-be taken out Chinese bee queen cells are cut off, and the queen cells are respectively arranged into the Chinese bee swarms needing the Chinese bee queens. The method has the advantage that the efficiency of cultivating Chinese bee queens in bulk is greatly improved.

The invention discloses a Chinese herbal medicinal preparation for preventing and treating Chinese sacbrood. The preparation consists of the following crude medicines in parts by mass: 26-30 parts of longhairy antenoron herb, 26-30 parts of selfheal, 26-30 parts of plantain herb and 14-18 parts of acanthopanax root. Experiment proves that the Chinese herbal medicinal preparation for preventing and treating Chinese sacbrood, when being fed to Chinese bee swarm, is good in palatability and popular in the bee swarm, and the Chinese herbal medicinal preparation has no influence to the reproductive capacity of the bee swarm and can remarkably reduce the incidence and disease index of Chinese sacbrood; the Chinese herbal medicinal preparation is easy to keep, long in shelf life, simple in prepare, convenient and simple to apply, and can be industrially produced.

The invention discloses a Chinese bee hive with an outer solid layer box body and an inner transparent layer box body; a solid outside layer is a bee hive of an existing structure or an underground honeycomb which can isolate light, insulate heat and prevent sunstroke. An inner transparent layer is a transparent box which is made of a novel thermoplastic transparent material in industrialized production. The box body of the inner transparent layer is used for receiving wax secreted by a bee for building the honeycomb. When the honeycomb in the box is checked, the situation in the honeycomb can be seen only by tearing off the outer layer box or lifting the inner layer box, so that the problem of the prior art that the situation in the honeycomb can be observed after taking off the cover and opening the box. In order to realize the practicality of the Chinese bee hive, the structure of the inner transparent layer bee hive is designed into a food steamer shape, the biological characteristics of Chinese bees are integrated, the complicated management in breeding Chinese bees with movable frames is omitted, and the Chinese bees are bred through a 'non-management' management method. According to the Chinese bee hive with the outer solid layer box body and the inner transparent layer box body, because the novel bee hive is made of the novel transparent material in the industrialized production, a bee hive observation function is realized, the effect of simplifying the bee observation is achieved, the simple operation of Chinese bee breeding is promoted, and a large-scale Chinese bee breeding process is promoted.

The invention discloses a main protein AccMRJP7 gene of Chinese royal jelly honey and an encoding protein of the gene, the gene has the DNA sequence shown in SEQ ID No. 5, and the encoding protein of the gene has the amino acid sequence shown in SEQ ID No. 6 and can be used for antibody preparation. The main protein AccMRJP7 gene of Chinese royal jelly honey and the encoding protein of the gene establish certain foundation for MRJP7 bioengineering application, particularly provides a new method for detecting MRJP protein family by using a MRJPs member antibody, which has wide usage in the research and production fields of royal jelly. The main protein AccMRJP7 gene of Chinese royal jelly honey and the encoding protein of the gene provide foundation for further research of MRJP-related protein active molecule mechanism as well as development of MRJPs-related functional food, diagnostic reagents and biological reagents.

The invention belongs to the field of bee breeding, and particularly relates to a bee breeding method. The method includes the following steps of firstly, feeding Italian queen bees with Chinese bee milk, incubating laid eggs into larvae, culturing the larvae into first-generation Italian queen bees, feeding the first-generation Italian queen bees with Chinese bee milk, incubating laid eggs into larvae, culturing the larvae into second-generation Italian queen bees, incubating eggs laid by the second-generation Italian queen bees into larvae, and placing the larvae in Chinese bee colonies to culture third-generation Italian queen bees. The obtained bee colonies are stable in character, resistant to diseases and pests, strong in mite resistance and high in honey yield.

The invention discloses a semicircular all-metal Chinese bee activity comb frame which is capable of enabling Chinese bees to form spherical bee clusters and suitable for the Chinese bees to store honey and produce honey in supers. The semicircular all-metal Chinese bee activity comb frame is made according to the following steps of: combining a concave metal upper beam and a semicircular metal lower frame via spot welding or bolts, and threading longitudinal and transverse thin iron wires into threading small holes. The comb frame has a semicircular lower part, is capable of enabling the Chinese bees to form spherical bee clusters and accords with the biological characteristics of Chinese bees; only the 1.2-1.5mm thick metal upper beam is suitable for the Chinese bees to store honey and produce honey in supers, so that the honey quality and output of the Chinese bees are improved and the structure is simple.

The invention discloses an immunity colloidal gold test strip for quickly detecting Chinese bee sac brood virus. The immunity colloidal gold test strip comprises a PVC (Polyvinyl Chloride) base plate, wherein a sample pad, a colloidal gold pad, a nitrocellulose membrane and a water absorption pad are sequentially fixed on the PVC base plate from left to right; a detection line and a quality control line mutually separated are arranged on the nitrocellulose membrane; the colloidal gold pad is marked with an anti-mouse infectious bursal disease virus capsid protein vp3 monoclonal antibody; the detection line on the nitrocellulose membrane is coated with another anti-mouse vp3 monoclonal antibody; the quality control line on the nitrocellulose membrane is coated with goat-anti-mouse IgG. The colloidal gold test strip is used for detecting the Chinese bee sac brood virus, and is high in detection specificity, simple, convenient and fast to operate, intuitive in result, and convenient for popularization and application.

The invention discloses a method for improving the yield and quality of Chinese bee honey. The Chinese bee has the advantages of capability of taking advantage of sporadic nectariferous plants, strong gathering power, high utilization efficiency, long period of honey gathering, high adaptability, and high resistance to mites and diseases. While, the Italian bee is larger than the Chinese bee and docile, has strong spawning and feeding capacity, is able to suddenly gather a large quantity of nectar sources, and has strong bleeding capacity. The method comprises the steps of putting the honeycomb spleens of raised Chinese bees in the bee hive of raised Italian bees to feed the Italian bees for 1-2 days and taking the honeycomb spleens back to the bee hive of the Chinese bees, wherein the honeycomb spleens contain Chinese bee egg spleens. The method combines the advantages of the Chinese bee and the Italian bee. Through Italian bee feeding, the adult Chinese bee is strong, the gathering and bleeding abilities of the Chinese bee are improved, and the yield and quality of honey are improved. The produced honey is deep yellow, has a unique taste, is mellow, and has high concentration, and the honey is sweet and delicious, and is of rich nutrition.

The invention discloses a method for attracting Chinese bees. The method includes the steps: (1) crushing 3g of dried osmanthus and 1-2 eliminated or dead Chinese queen bees, adding 100g of water, and soaking the mixture for 24-30h after the dried osmanthus, the Chinese queen bees and the water are uniformly mixed; (2) uniformly mixing pollen and 52-degree corn wine according to the weight ratio of 1:3, and soaking the pollen and the corn wine for 5-8h; (3) toasting a beehive to reach 60 DEG C, uniformly coating the inner wall of the beehive with 10g of beeswax or old half-comb honey of the Chinese bees; (4) uniformly mixing honey and osmanthus liquid in the first step according to the weight ratio of 1:15-20, spraying the mixed honey and osmanthus liquid on the inner wall of the beehive, and then drying the beehive; (5) spraying 1g of pollen and corn wine soaking liquid in the second step on the wall of the beehive, and then drying the beehive; (6) selecting sunny, lee and dry cave mouths, the lower portions of rocks and trees or the upper portions of tree branches for placing the beehive. The method is short in consumed time and high in efficiency.

The invention relates to a method for controlling CSBV (Chinese Bee Sacbrood Virus) by utilizing an RNA interference technology. The method comprises the following steps: synthesizing dsRdRp through a bacteria prokaryotic induction expression method by utilizing an RNA-dependent RNA polymerase (RdRp) gene which is specific to the CSBV and related to virus replication, and adding double-strand RNA into feed for feeding larva of 1-2 days. The double-strand RNA is expressed by Escherichia coli induction, is low in cost and has a good curing effect for controlling the CSBV. The feeding concentration of dsRdRp is 0.5 microgram / ml, and the pupation rate and eclosion rate of the larva are respectively 70 percent and 55 percent and are obviously improved compared with the pupation rate of 10 percent and the eclosion rate of 0 percent in the larva fed of dsGFP.

The invention discloses a hybrid queen bee breeding method. The hybrid queen bee breeding method comprises the steps that (1), eggs produced by Italian queens are incubated for three days in a beehiveinto larvae, queen cells are made with beewax, the incubated Italian larvae are transferred to the queen cells respectively and then placed into a Chinese bee box to be subjected to Italian bee breeding, and the Italian larvae get involved in Chinese bee ingredients; (2), eggs produced by the Chinese bees are incubated for three days in the beehive into larvae, the larvae are transferred to the queen cells and then placed into an Italian box to be subjected to Chinese bee breeding, and the Chinese bees get involved in Italian bee ingredients; (3), the Italian larvae in the Chinese bee box arebred to become Italian bee female queens, and the Chinese bee larvae in the Italian bee box are bred to become Chinese bee male queens; (4), copulation is conducted, the born Italian bee female queens are guided to copulate and spawn with the Chinese bee male queens mutually, and after 20 days of incubation, hybrid bees are bred. By the adoption of the hybrid queen bee breeding method, the bred bees combine advantages of the Italian bees and the Chinese bees.