26 results about "Chinese bee" patented technology

The utility model discloses an auxiliary device for breeding apis cerana in a soil slope or a ridge, which is characterized by comprising a box-shaped main body (1), a front door (2) of the box-shaped main body, an exhaust pipe external connecting pipe (3), an air inlet and water outlet pipe external connecting pipe (3) and a nest door external connecting device. When the auxiliary device is used, a soil hole with a proper size is dug in a soil slope or a ridge, the box-shaped main body of the auxiliary device is put in the soil hole, a beehive is put in the box-shaped main body, a beehive door external connection device is installed, and then a front door, an exhaust pipe external connection pipe and an air inlet drainage pipe external connection pipe of the box-shaped main body are installed. The beehive placed in the auxiliary device can obtain more excellent in-hole production conditions through the auxiliary device, and healthy, safe and efficient production of Chinese bees is guaranteed.

The utility model discloses an apis cerana feeding device, which relates to the field of apis cerana feeding devices and comprises a food storage box used for storing sweet water, pollen or other bee food. The feeding trough is used for containing food for bees to eat, and the bottom of the food storage box is provided with a food feeding pipe communicating with the upper portion of the feeding trough; the automatic control module comprises a quantity control electromagnetic valve, the quantity control electromagnetic valve is arranged on the feeding pipe, and when the quantity control electromagnetic valve is opened, bee food stored in the food storage box can be fed into the feeding trough through the feeding pipe. The automatic control module can automatically adjust the feeding amount according to the activity condition of bees and the environmental condition, it is ensured that each bee can obtain enough food, and the feeding efficiency is improved; the frequency of manually putting food is reduced, the stress response of bees is reduced, and the health level and productivity of bee colonies are improved; the double-layer heat preservation storage box and the automatic control module ensure that food is fresh and safe, and bee colony diseases caused by food deterioration are reduced.

The invention discloses an application of a heat-resistant related gene Hsc70-4 of apis cerana and an encoding protein of the heat-resistant related gene Hsc70-4, and belongs to the technical field of cytobiology and molecular biology. The invention verifies that silent Hsc70-4 can reduce the survival rate of Chinese bees under heat stress, and overexpressed Hsc70-4 can increase the survival rate of drosophila S2 cells under heat stress. Induced expression of Hsc70-4 is helpful for reducing oxidative damage of heat stress to apis cerana and improving heat resistance of apis cerana. In view of the heat resistance of the Hsc70-4, overexpression of the Hsc70-4 in bees or economic animals is expected to be realized through a transgenic technology in the future, so that the heat resistance of the Hsc70-4 is improved, the yield of the Hsc70-4 under a heat stress condition is further improved, and a new gene target is provided for coping with ecological and agricultural challenges under a global warming background.

The invention relates to the technical field of gene detection, in particular to a primer group for detecting a beta-GBP gene of apis cerana and application of the primer group, and the primer group comprises an upstream primer CDS-Acbeta-GBP-1-F with a nucleotide sequence as shown in SEQ ID No.1; the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-1-R is as shown in SEQ ID No. 2; the nucleotide sequence of the upstream primer CDS-Acbeta-GBP-2 is as shown in SEQ ID No.3, and the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-2-R is as shown in SEQ ID No.4; the nucleotide sequence of the upstream primer CDS-Acbeta-GBP-3-F is as shown in SEQ ID No.5, and the nucleotide sequence of the downstream primer CDS-Acbeta-GBP-3-R is as shown in SEQ ID No.6. The invention further discloses a kit for detecting the content of the CDS-Acbeta-GBP-3-F. The Ac beta-GBP gene found by the research can be used as a molecular breeding target spot, disease-resistant bee species highly expressing the gene can be screened by detecting the Ac beta-GBP gene through the primer pair provided by the invention, the method for screening the chalkbrood disease-resistant bee species is enriched, and meanwhile, the Ac beta-GBP provides a new view angle and a new target spot for illuminating a chalkbrood disease-resistant molecular mechanism of Chinese bees, so that the application prospect is wide. And a theoretical basis is laid for developing a novel bee disease prevention and control technology based on RNA interference.

The present invention relates to the technical field of bee breeding, and particularly relates to a method for mixed feeding of Italian bees and Chinese bees. The method for mixed feeding of Italian bees and Chinese bees comprises the following steps: S1: Raise the Italian bee colony at one side position of a double-queen colony beehive; S2: After 2 to 3 days, transfer the Chinese bees into the beehive and raise them at the other side position, and an adjustable queen excluder is installed in the middle of the beehive to separate the two bee colonies; S3: Open both nest entrances, and both bee colonies can freely enter and exit their respective nest entrances, recognize the hive, and collect; S4: After raising for a period of time, adjust the aperture of the queen excluder to 0.2 cm and spray a bee colony odor reduction liquid; S5: After 3 to 5 days, adjust the aperture of the queen excluder to 0.38 cm; S6: After a period of time, remove the escape prevention piece on the nest entrance of the Chinese bee colony to make it more free for the Chinese bees to enter and exit; S7: Add a super in the season with sufficient honey and pollen sources. The present invention provides a method for mixed feeding of Italian bees and Chinese bees, which can not only improve the mite resistance of the Italian bee colony, but also reduce the risk of the Chinese bee colony being infected with wax moths.

The utility model discloses an apis cerana multi-queen bee breeding box in the technical field of apis cerana breeding, which comprises two sub-hive boxes, at least one side of each sub-hive box is provided with an opening, a partition plate is detachably connected to the opening, the two sub-hive boxes are assembled by connecting the openings of the two sub-hive boxes through assembling pieces, the partition plate is provided with a ventilation structure, and the ventilation structure is connected with the opening of each sub-hive box. The sub-hive boxes are spliced, so that a plurality of queen bees can be conveniently combined, a group of bees can be detached at any time to form a plurality of queen bee combination with other hive bodies, a ventilation structure on a partition plate is arranged, and structures such as queen bee separation plates and gauze plates do not need to be erected when smell is mixed.

The invention relates to the technical field of bee breeding, and particularly discloses breeding equipment for Chinese bees. Comprising an arc-shaped feeding trough, and a liquid passing pipe is arranged in the arc-shaped feeding trough; an overflow hole is formed in the outer surface of the liquid passing pipe, the top of the liquid passing pipe communicates with a top connecting pipe, the top of the top connecting pipe communicates with a pushing and pressing mechanism, a first round block is arranged on the inner wall of the liquid passing pipe, one side of the first round block is fixedly connected with an extension spring, and the end, away from the first round block, of the extension spring is fixedly connected with a second round block; according to the breeding equipment for the apis cerana, an overflow hole which is small in hole diameter and not convenient for fluid food to directly flow out is formed in the liquid passing pipe, so that the liquid passing pipe can be filled with the fluid food; the situation that the feeding area of the fluid food is not uniform and the bee breeding effect is affected due to the fact that the fluid food entering the liquid passing pipe leaks outwards when the liquid passing pipe is not filled and distributed at all positions of the arc-shaped feeding trough is prevented.

The invention relates to the technical field of honey identification, in particular to a multi-omics-based Changbai Mountain Chinese bee honey characteristic component analysis method. According to the method, mass spectrometric detection is carried out on the Changbai Mountain apis cerana honey and the reference substance honey, qualitative and quantitative analysis is carried out on metabolites and proteins of the honey, metabonomics and proteomics information is obtained, and metabonomics and proteomics difference information of the Changbai Mountain apis cerana honey and the reference substance honey is obtained; the method comprises the following steps: carrying out functional annotation on the Chinese bee honey, finding out KEGG pathways annotated by two groups of schools by utilizing KEGG database pathway analysis, carrying out expression correlation analysis and O2PLS analysis on the KEGG pathways annotated by the two groups of schools, and screening characteristic components of the Chinese bee honey in the Changbai Mountain; potential characteristic substances of the Changbai Mountain apis cerana honey are obtained through screening, and a scientific basis is further provided for origin traceability and quality identification of the apis cerana honey.

The invention discloses an apis cerana DnaJC28 gene as well as an encoding protein and application thereof, and belongs to the technical field of cytobiology and molecular biology. The invention finds that apis cerana DnaJC28 is positioned in cytoplasm through research for the first time. The short-term heat stress and the long-term heat stress can induce the expression of the apis cerana DnaJC28, the silent DnaJC28 can reduce the survival rate of the apis cerana under the heat stress, and the heterologous overexpression apis cerana DnaJC28 can increase the survival rate of the drosophila S2 cells under the heat stress. A plurality of antioxidant genes can partially make up the function of DnaJC28 under heat stress, and oxidative damage caused by heat stress to Chinese bees is reduced. The gene can be used as a novel heat-resistant gene target, the gene is over-expressed in other economic animals in the future, the yield and the heat resistance of the gene under the heat stress condition are expected to be improved, and a sustainable solution is provided for livestock production under the climate change.

The utility model relates to the technical field of agricultural cultivation, and particularly discloses a novel seed and honey separation type Chinese bee cultivation movable frame box which comprises an original box, a super is installed on the upper end face of the original box, first sliding grooves are formed in the front side and the rear side of the upper end face of the original box, and two supporting tables distributed front and back are fixedly connected to the left side of the original box. Second sliding grooves communicating with the first sliding grooves are formed in the upper end faces of the two supporting tables correspondingly, when honey needs to be taken, the super is pushed leftwards, the super moves leftwards on the first sliding grooves and the second sliding grooves through the sliding tables till the super moves to the position above the supporting tables, then an original box can be exposed for honey taking, and therefore the purposes that the super is convenient to move, and the honey taking efficiency is improved are achieved. Honey is taken from the original box below, manual carrying of the box body is avoided, the lower end face of the original box can be protected through the supporting plate, and the situation that the service life of the original box is affected due to the fact that moisture on the ground makes direct contact with the original box can be avoided through the damp-proof plate.

The utility model discloses a combined type vertical Chinese bee hive with movable hives, which relates to the field of hives and comprises a plurality of vertical hive bodies which are stacked, each vertical hive body comprises two symmetrically arranged hive body side plates, one side of each of the two hive body side plates is clamped with a hive body front plate, and the opposite side of each of the two hive body side plates is clamped with a hive body rear plate. Grooves are symmetrically formed in the tops of the opposite sides of the box body front plate and the box body rear plate, the vertical beehive covers are installed on the tops of the vertical beehive bodies in a stacked mode, and the queen excluders are arranged between every two adjacent vertical beehive bodies. According to the combined vertical Chinese bee hive with the movable hive bodies, the vertical Chinese bee hive bodies which are arranged in a stacked mode are formed, each hive body is formed by connecting the side plates, the front plates, the rear plates and other parts in a clamping mode, the modular design enables the hive to be assembled and disassembled easily and rapidly, and daily management and maintenance of beekeepers are facilitated.

The method comprises the following steps: screening virgin queen bees and drones of Chinese bees naturally bred in a southwest origin area, and domesticated first target drones and second target drones, and establishing a hybrid combination; taking the first target drone as a male parent, hybridizing with the native Chinese bee virgin queen bee as a female parent to obtain a first target hybrid filial generation, and taking the second target drone as a male parent, hybridizing with the native Chinese bee virgin queen bee as a female parent to obtain a second target hybrid filial generation; backcrossing male apis cerana in the origin place as a male parent and excellent virgin queen bees of the hybrid filial generations of the first target and the second target as female parents respectively to obtain first and second backcross filial generations respectively; respectively carrying out composite hybridization on the first target and second target backcross offspring excellent drones and excellent virgin queen bees to respectively obtain first and second composite cross offspring Chinese bees; through multi-generation multi-cross breeding, excellent swarms with high reproduction rate, high honey yield, strong stress resistance and genetic stability are obtained through screening.

The utility model discloses a multifunctional soil breeding frame, and belongs to the technical field of bee breeding. The nest frame comprises a concave upper frame beam, a top cover plate, a left frame, a right frame, a lower frame beam, sliding block units, steel sticks and screws, the concave upper frame beam and the top cover plate form a feeder, a concave groove is reserved in the bottom of the feeder, the four frames are connected through mortise and tenon joint structures and are reinforced through the screws, and a plurality of sets of sliding blocks are buckled on the left frame and the right frame. Steel sticks are placed at the notches of the same horizontal sliding block to form a frame with the interior capable of moving flexibly; the beehive is provided with the feeder, the groove for installing the guide foundation is reserved, the sliding block units can be flexibly detached and moved, completeness of remaining honeycombs after honey taking is guaranteed, an environment for new honeycombs to produce birth is created for Chinese bees, operation is easy, the problems that bee growers check bees, change queen bees, swarming bees, frequently open the beehive and add frames, and hurt the birth and the bees after honey taking are solved, and the beehive is suitable for being popularized and used. A plurality of frames can be added at a time, the original ecological habit of ball holding development of bees is truly restored, the cost is effectively saved, and the capacity of bee colonies is improved.

The invention provides a method for controlling crystallization of Chinese bee honey, which comprises heat treatment and ultrasonic treatment, the ultrasonic treatment is carried out after the heat treatment, the heat treatment temperature is 60 DEG C, the constant heat treatment temperature is 60 DEG C for 20 minutes, the nominal frequency of the ultrasonic treatment is 40 kHz, the ultrasonic bath temperature is controlled at 25 + / -5 DEG C, and the ultrasonic treatment time is 20 minutes. Compared with traditional heat treatment, the synergistic process of '60 DEG C / 20 min heat treatment + 40 kHz / 20 min ultrasonic treatment 'has the more remarkable comprehensive advantages that the crystallization control effect is better, active ingredients are better reserved, less harmful substances (HMF) are generated, the microorganism inactivation efficiency is higher, the sensory and physical characteristics are more stable, and the energy consumption and the processing plant efficiency are better.

The utility model relates to the technical field of mating boxes, and discloses an apis cerana mating box which solves the problem that frames in an existing mating box are inconvenient to adaptively arrange, and comprises a cover body arranged at the top end of a box body, a plurality of frames arranged in the box body, and a first lap plate fixedly arranged at the top end of one side of each frame. A first lap-joint plate is fixedly arranged on one side of the frame, a first half groove is formed between the first lap-joint plate and the frame, a second lap-joint plate is fixedly arranged on the other side of the frame, a second half groove is formed between the second lap-joint plate and the frame, a first threaded hole is formed in the middle of the top end of the frame, second threaded holes are formed in the first lap-joint plate and the second lap-joint plate, and locking bolts are arranged on the second threaded holes. A rectangular supporting frame body matched with the frame is fixedly arranged at the top end of the inner side wall of the box body; by means of the mating box, a single frame can be longitudinally arranged or two frames can be spliced to be transversely arranged according to needs, and adaptability is improved.

The invention relates to the technical field of double-queen cultivation of southern Yunnan Chinese bees, in particular to a method of a double-queen cultivation technology of southern Yunnan Chinese bees, which comprises the following steps: preferably selecting a local high-yield disease-resistant bee species, intervening a same-period queen cell into a standardized bee colony, constructing a double-area structure by using a vertical partition plate, applying honey liquid to the same-age queen bees, and then intervening in different areas. The size of the hive entrance is dynamically adjusted according to the group potential difference, worker bee channels are opened in stages, and the partition plates are removed to combine the double-area swarms. The elsholtzia rugulosa plant extract is mixed in a gradient manner to replace alcohol, so that the rejection behavior of worker bees is avoided; group potential balance is controlled in a linkage mode through dynamic hive entrance regulation and control and cover sealing spleen threshold value exchange; the staged partition plate channels are opened, the worker-bee interaction rate larger than or equal to 70% serves as a switching node, and two-area pheromone progressive fusion is achieved; through combination of overwintering bee group temperature closed-loop control and queen bee spacing precise management, the double-queen coexistence stability is finally improved to 90% or above, and the upper limit of the bee group potential in southern Yunnan is broken through to the scale of 12 honeybees.

The invention relates to a preparation method and application of an immune response activating protein PPAN for preventing and treating Chinese bee cystic larva viruses, which comprises the following steps: amplifying from cDNA of Chinese bee larvae by using PCR (Polymerase Chain Reaction) to obtain PPAN genes of the Chinese bee larvae, connecting a PCR product to a pGEX-4T-1 vector to obtain positive recombinant plasmid pGEX-4T-1-PPAN, and transforming the positive recombinant plasmid into BL21 (DE3) competent cells to obtain the immune response activating protein PPAN for preventing and treating the Chinese bee cystic larva viruses. Inoculating the obtained bacterial liquid into an ampicillin-containing LB culture medium, and carrying out shaking culture; and transferring into an ampicillin-containing liquid LB culture medium, and carrying out mass induction and purification to obtain the immune response activating protein PPAN. The immune response activating protein PPAN has the advantages of being easy to prepare, low in production cost, convenient to use, rapid in effect, high in cure rate, high in specificity, free of medicine residues and convenient to store, and the immune response activating protein PPAN is applied to the Chinese bee sacbrood virus resisting medicine.

The invention discloses a method for increasing the comb making rate of a Chinese bee movable frame with a foundation, which comprises the following steps of: S1, selecting a Chinese bee and Italian bee colony with good colony potential and normal spawning of a queen bee, and feeding the Chinese bee and Italian bee colony in a beehive; s2, during sunset, adding a frame for mounting a Chinese honeycomb foundation into a nest of the swarms of Italian bees, and feeding syrup to the swarms of Italian bees; after the apis mellifera constructs a semi-finished honeycomb, the semi-finished honeycomb is taken out; s3, semi-finished honeycombs taken out of the Italian honeycombs are added into the Chinese bee colony nests for Chinese bee colonies to build honeycombs in a relay mode, the Chinese bee colonies are fed with syrup, and spleen building is completed by the Chinese bee colonies. By means of the method, the Chinese bee finished product honeycomb of the Langshi standard box can be built within 28 hours.

The utility model discloses a horizontal Chinese bee hive for bee breeding, which relates to the field of bee breeding and comprises a honeycomb for providing a place for inhabiting and breeding a bee colony; the beehive is mounted outside the honeycomb and used for protecting the honeycomb inside the beehive; the beehive comprises two symmetrically-arranged heat preservation plates which are installed on the inner wall of the beehive in a sliding mode. The two groups of frames are symmetrically arranged and are mounted in the beehive; the vertical partition plates are fixedly connected with clamping grooves in the inner wall of the beehive; each of the two groups of symmetrically arranged frames comprises a frame transverse hanger and a frame transverse hanger; the frame transverse hangers are connected with the inner wall of the beehive in a sliding manner; the honeycomb is fixedly mounted at the bottom of the frame transverse hanger; according to the horizontal Chinese bee hive for bee breeding, through the design of flexible disassembly, a bee farmer can conveniently replace damaged parts of the hive, and through the movable arrangement of the honeycomb, the bee farmer can adjust the number and position of the honeycomb frames according to needs.

The invention discloses an automatic queen bee changing device and method for traditional feeding of apis cerana. The automatic queen bee changing device for traditional Chinese bee feeding comprises a hive, the hive is provided with a hive entrance, a queen bee cage is arranged at the top of the hive, a queen bee automatic separator communicated with the hive entrance is arranged at the hive entrance, and the queen bee automatic separator is communicated with the queen bee cage through a hose through which bees can normally pass. In addition, a queen bee check baffle is arranged at an inlet of the queen bee cage. The non-return baffles are two non-return elastic pieces arranged at a queen bee inlet of the queen bee cage, and the two non-return elastic pieces are oppositely arranged, obliquely stretch out towards the interior of the queen bee cage and form an inlet triangle. And the outside of the queen bee cage is covered with a light shielding part. By means of the device and the method, the queen bee can be stably replaced within about one year, it is guaranteed that the egg laying force of the queen bee cannot be reduced, and good group potential and collection enthusiasm are maintained.

The invention discloses a Chinese bee drone semen extraction device and an efficient extraction method, the semen extraction device comprises an extraction type piston, a drone imbedding device, a semen collection channel and a movable semen collection table, and the extraction type piston is arranged at an upper port of the drone imbedding device; a seminal fluid collecting channel is arranged below the drone imbedding device, and an EP pipe is arranged on a movable seminal fluid collecting table below the seminal fluid collecting channel and used for receiving extracted seminal fluid. The semen extraction and enrichment method comprises the following steps: placing a drone into the drone fixator, keeping the abdomen upward and enabling 2-3 back plates at the tail part of the drone to extend out of the fixing device, enabling the chest and the abdomen of the drone to directly face an electric shock opening, and then installing an extraction type piston to stabilize the position of the drone; and the chest and the abdomen of the drone are electrically shocked simultaneously by a 220V voltage contact, so that the cutin, the mucilage gland and the positive stem bulb of the drone are completely turned outwards, and then the sperm extraction by electric shock is completed. After drone electric shock operation is completed, a basic auxiliary buffer solution is used for flushing seminal fluid on the outer side of a positive stem ball subjected to electric shock extroversion, the seminal fluid flows into an EP tube, the seminal fluid collecting method guarantees the activity and the number of sperms, and impurities in the extracted seminal fluid are remarkably reduced; and then freezing and centrifuging to obtain the high-enrichment and high-activity seminal fluid.

The utility model discloses a queen bee rearing platform base suitable for apis cerana, and relates to the technical field of bee breeding equipment, the queen bee rearing platform base comprises a platform base and a platform base sheet, an inner cavity of the platform base is of a big-end-up structure, the diameter of the top of the inner cavity is 6.8-7.2 mm, the diameter of the bottom of the inner cavity is 6.1-6.7 mm, and the height of the inner cavity is 8-9 mm; the base piece comprises a bearing portion and an inserting portion which are connected with each other, the bearing portion is used for bearing the base, the inserting portion is used for being connected with a honeycomb, and the bottom face of the base is fixedly connected with the upper surface of the bearing portion. The diameter of the top of the inner cavity of the platform base is 6.8-7.2 mm, the diameter of the bottom of the inner cavity is 6.1-6.7 mm, and the height of the inner cavity is 8-9 mm, so that compared with the size of an existing platform base, the platform base provided by the utility model is more in line with the biological characteristics of Chinese bees, thereby being more beneficial to queen bee rearing of the Chinese bees. The contact area with the queen breeding frame supporting strips is increased by arranging the table base piece located at the bottom of the table base, and therefore the stability of the table base is improved.

The application discloses a method for breeding strong colonies of honeybee larvae with high efficiency, and belongs to the technical field of honeybee breeding. The method comprises the following steps: after overwintering, two colonies are put into one beehive, and the colonies are separated by a queen separator for outdoor spring breeding; during the outdoor spring breeding, the queenless larvae in the colonies are taken out, put into a beehive with a feeding device at the bottom for indoor incubation, and a queenless feeding colony is formed; after the queenless feeding colony is completely covered, the queenless feeding colony is moved out of the room, and is gradually added into the outdoor breeding colony, so that the queenless feeding colony is hatched out of the room; the indoor incubation step is repeated until the external temperature is stable, and honey powder sources appear. The method has obvious effects on breeding strong colonies and increasing production, has important significance for improving the economic benefits of the beekeeping industry, and provides a guarantee for the development of the Chinese bee industry.

The invention belongs to the technical field of honey detection, and particularly relates to a method for identifying Chinese mountain sapium sebiferum honey and Italian mountain sapium sebiferum honey based on the amino acid ratio. The method for identifying the Chinese alpine mountain sapium sebiferum honey and the Chinese alpine mountain sapium sebiferum honey provided by the invention comprises the step of identifying the Chinese alpine mountain sapium sebiferum honey and the Chinese alpine mountain sapium sebiferum honey on the basis of the ratio of the proline content in the total content of free amino acids and / or the ratio of the phenylalanine content in the total content of the free amino acids in a honey sample. The method has the advantages of simplicity and convenience in operation, rapidness, high accuracy and the like, can realize rapid identification of the source of the Sapium discolor honey species, provides technical support for authenticity identification of honey, and has important significance in promoting healthy development of the honey industry.

The invention relates to the technical field of South Yunnan Chinese bee breeding, in particular to a South Yunnan Chinese bee improved breed breeding method which comprises the following steps: screening a queen bee and a parent group, preferably selecting a female group and a parent group with stable characters, and carrying out artificial insemination on the queen bee of the female group to preserve high-quality genetic materials; drone breeding is synchronously regulated and controlled, male group male bee breeding is started in advance, and the drone quality is enhanced through bee quantity densification, feeding optimization and special drone comb management; nutrition of the female group in the larva stage is enhanced, feeding space of the female group is adjusted, protein feed is supplemented, and sufficient nutrition in the larva stage is ensured. According to the method, the quality defect rate of the queen bee is reduced to 5% or below from 40% of a traditional method through genetic resource dynamic updating, larva nutrition precise regulation and control and environment adaptation parameter design, and a quantifiable technical scheme is provided for resource protection and industrial upgrading of the southern Yunnan Chinese bees.