The invention relates to a breeding method of queen bees. The method comprises the specific steps of selection of a field, an environment, selection of a bee species, selection and demands of a queenbreeding colony, organization of the queen breeding colony, management of the queen breeding colony, larval transplantation for queen breeding, larval transplantation, organization of a coupling colony, a queen introduction method, a management method of the coupling colony, coupling control over Chinese bees, queen replacement time of the Chinese bees and a queen replacement method of the Chinesebees. By means of the method, a proper environment and rich nutrient conditions are created, according to the number and time which are required by a plan, batched good-quality queen bees are bred, and accordingly the aims of colony multiplication and good species popularization are achieved. The sexual maturity periods of drones and the queen bees are reasonably selected, and the sexual maturityperiod of the bred queen bees can be matched with the puberty of the drones. During queen breeding, a colony which is poor in swarming performance and good in species character and can maintain the large swarm is reasonably selected, used as a breeding colony and used for breeding new queen bees and drones respectively. By reasonably using queen replacement of the queen bees and factors such as the climate, the honey source, the population of the bee colony and the behavior and physiological states of the queen bees, introduction of the queen bees easily succeeds.