The invention discloses equipment for accelerating natural maturity of honey and a using method thereof, and belongs to the professional technical field of honey production. The equipment comprises anegative pressure fan, a louver, a thermostat, a hygrometer-thermometer, a diaphragm plate, an air inlet, a stainless steel screen, a drying agent box I, a thermometer, an air inlet pipe, a drying agent box II, a thermostatic water tank, a water thermometer, a temperature control electric heating pipe, a water inlet and a water outlet. The using method comprises the following steps: a) filling honey combs with honey in a swarm at a honey flow period; b) opening the electric heating pipe, and adjusting the temperature of water in the thermostatic water tank; c) opening the negative pressure fan, and adjusting the temperature and relative humidity in the thermostat; d) putting the honey combs in the thermostat, and maintaining the temperature and the relative humidity to be constant; e) placing the honey combs in the thermostat for 24 to 48h; f) taking out the honey combs from the thermostat, putting the honey combs back to a beehive, and covering the beehive. The natural maturity time of honey can be shortened, and the yield of mature honey is improved when the quality, nutrition and flavor of the honey are ensured.