Method for the detection of potential toxicity of gastrotoxic insecticides or genetically modified insecticidal proteins to larvae of Apis mellifera

Abstract

The invention provides a simple and sensitive method for detecting direct toxic influence of stomach insecticides or transgenic insecticidal proteins on Italian bee larvae. The method comprises the following steps: uniformly mixing a tested insecticidal compound with artificial feed and directly feeding to the Italian bee larvae; and comparing and analyzing important life indexes, such as survival rate, developmental duration, prepupa weight and eclosion rate, in a processed group and a control group of the Italian bee larvae by taking the artificial feed, to which a soybean trypsin inhibitor (SBTI) with the concentration of 0.1%(w:w) is added, as positive control and taking pure artificial feed as blank control so as to detect the potential toxicity of the tested insecticidal compound. Due to the adoption of the method, the toxicity of the SBTI with different concentrations on the Italian bee larvae is detected; a result shows an obvious dose-effect relation between different life parameters of the Italian bee larvae and the concentration of the SBTI in the feed so that the method achieves effectiveness and sensitivity.

Description

Technical field [0001] The invention relates to a method for detecting the toxicity of gastric toxic insecticides or transgenic insecticidal proteins on Italian honeybee larvae. Background technique [0002] Bees are a very important economic and ecological insect (Babendreier et al., 2004). Bees rely on collecting nectar and pollen to survive and multiply, and objectively play a role in pollinating plants and promoting the balanced development of natural ecology. This makes a huge contribution to the natural world and is unmatched by humans and other organisms. Bees such as Italian honeybees use plant pollen as their main source of protein, and also collect plant nectar and honeydew secreted by aphids or other insects. In this way, during feeding, it may be exposed to insecticides or exogenous insecticidal proteins expressed by transgenic insect-resistant plants, such as Bt proteins. Ingestion of transgenic insect-resistant plant pollen may be the only way for honeybees to be ...

AI Technical Summary

Problems solved by technology

[0010] Based on the above, the current inspection methods for detecting the direct toxic effects of gastric toxic insecticides or transgenic insecticidal proteins on Italian honeybee larvae are not perfect, and researchers have not found evidence and instructions for improving the existing technology. Therefore, it is necessary to provide simple and sensitive Examination methods to detect the direct toxic effects of gastrotoxic insecticides or genetically modified insecticidal proteins on Italian honeybee larvae are quite difficult

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