Method for detecting potential toxicity of stomach insecticides or transgenic insecticidal proteins on Italian bee larvae

Abstract

The invention provides a simple and sensitive method for detecting direct toxic influence of stomach insecticides or transgenic insecticidal proteins on Italian bee larvae. The method comprises the following steps: uniformly mixing a tested insecticidal compound with artificial feed and directly feeding to the Italian bee larvae; and comparing and analyzing important life indexes, such as survival rate, developmental duration, prepupa weight and eclosion rate, in a processed group and a control group of the Italian bee larvae by taking the artificial feed, to which a soybean trypsin inhibitor (SBTI) with the concentration of 0.1%(w:w) is added, as positive control and taking pure artificial feed as blank control so as to detect the potential toxicity of the tested insecticidal compound. Due to the adoption of the method, the toxicity of the SBTI with different concentrations on the Italian bee larvae is detected; a result shows an obvious dose-effect relation between different life parameters of the Italian bee larvae and the concentration of the SBTI in the feed so that the method achieves effectiveness and sensitivity.

Description

technical field [0001] The invention relates to a detection method for the toxic effects of gastric toxic insecticides or transgenic insecticidal proteins on Italian honeybee larvae. Background technique [0002] Honey bees are economically and ecologically important insects (Babendreier et al., 2004). Bees rely on collecting nectar and pollen to survive and reproduce, and objectively play a role in pollinating plants and promoting the balanced development of natural ecology. This is a huge contribution to nature, which is unmatched by humans and other creatures. Honey bees, such as Apis mellifera, use plant pollen as their main source of protein, but they also collect plant nectar and honeydew secreted by aphids or other insects. In this way, the feeding process may be exposed to insecticides or exogenous insecticidal proteins expressed by transgenic insect-resistant plants, such as Bt proteins. Feeding the pollen of transgenic insect-resistant plants may be the only way ...

AI Technical Summary

Problems solved by technology

[0010] Based on the above, the current inspection methods for detecting the direct toxic effects of gastric toxic insecticides or transgenic insecticidal proteins on Italian honeybee larvae are not perfect, and researchers have not found evidence and instructions for improving the existing technology. Therefore, it is necessary to provide simple and sensitive Examination methods to detect the direct toxic effects of gastrotoxic insecticides or genetically modified insecticidal proteins on Italian honeybee larvae are quite difficult

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