Method for identifying types of bee venoms through biogenic amine as feature mark

A technology of biogenic amines and markers, applied in the field of bee analysis, can solve the problems of endangering the health of experimenters, cumbersome derivatization process, no bee venom classification, etc., and achieve accurate and reliable measurement results, environmental friendliness, and easy operation.

Active Publication Date: 2016-07-06
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

Although derivatization can greatly improve the sensitivity, the derivatization process is cumbersome and time-consuming, and requires toxic derivatization reagents such as dansyl chloride, OPA, DMQC-QSU, CNBF, NQS, AQC, etc., which may endanger the health of experimenters.
[0009] In addition, because the amount of bee venom samples is very small, and the dangers such as bee stings are likely to occur during the collection process, there is currently no research on the classification of bee venom at home and abroad.

Method used

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  • Method for identifying types of bee venoms through biogenic amine as feature mark
  • Method for identifying types of bee venoms through biogenic amine as feature mark
  • Method for identifying types of bee venoms through biogenic amine as feature mark

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Embodiment 1

[0060] (1) Preparation of standard stock solution: Accurately weigh 10.0mg of histamine, dopamine, serotonin, epinephrine, and norepinephrine respectively, place them in a 10mL volumetric flask, dissolve them in pure water and make to volume, and shake well to obtain 1.0mg / mL standard stock solution, sealed, stored at -4°C (stable for 6 months).

[0061] (2) Preparation of mixed standard working solutions: Take an appropriate amount of each standard stock solution in the same volumetric flask, dilute to volume with pure water, and prepare the following series of standard working solutions: 1ng / mL, 5ng / mL, 10ng / mL, 50ng / mL mL, 100ng / mL, 500ng / mL and 1000ng / mL, sealed, and stored in a 4°C refrigerator for later use (stable for 1 month).

[0062] (3) Pretreatment of bee venom samples: Weigh 5.0±0.1mg of bee venom samples, place them in a 5mL centrifuge tube with stopper, add 2mL of pure water, vortex for 10min to mix, centrifuge at 1000rpm and draw part of the supernatant from th...

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Abstract

The invention relates to application of biogenic amine serving as a feature mark in bee venom type identification and further relates to a method for identifying types of bee venoms through the biogenic amine as the feature mark by means of HPLC/MS/MS methods. According to the method, one or more of histamine, dopamine, serotonin, epinephrine and noradrenaline serves as the feature mark, one or more of Italian bee venom, royal jelly high-yielding bee venom, Anatolia bee venom, Caucasia bee venom, Carniolan bee venom and Carpathian bee venom can be precisely identified. The method can efficiently, stably and accurately identify six types of bee venoms, and has significance on reinforcing study on bee venom type identification, exploring bee venom traceability information, developing traditional Chinese medicine, improving bee venom quality in China and guaranteeing health of consumers.

Description

technical field [0001] The invention relates to the technical field of honeybee analysis, in particular to a method for identifying bee venom species using biogenic amines as characteristic markers. Background technique [0002] Bee venom is a poisonous liquid secreted by the venom glands and accessory venom glands of bees and stored in the venom sac. When in a dangerous situation, the bee will inject bee venom into the body of the human body or the predator through the poisonous needle. This venom is easy to cause allergic reactions in the human body, and in severe cases, it is life-threatening. Bee venom contains various active compounds such as active enzymes, polypeptides, carbohydrates, lipids, amino acids and amines, so it has various pharmacological properties and biological activities such as anti-cancer, anti-radiation, antibacterial, anti-inflammatory and anti-mutation. In traditional Chinese medicine, people successfully use bee venom therapy to relieve chronic p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 周金慧李熠张文文吴黎明牛庆生张金振金玥王琳赵文
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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