A biological weed killer and preparation process thereof

A herbicide and biological technology, applied in the fields of botanical equipment and methods, biocides, herbicides and algicides, etc., can solve the problems of the scarcity of biological herbicides, difficult adaptation to ecological conditions, and poor effects, etc. The effect of high spore yield, low cost and easy availability of raw materials

Inactive Publication Date: 2007-07-11
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to propose a method aimed at the disadvantages caused by the long-term use of chemical herbicides for malignant weeds-Digits crabgrass, and the defects of existing biological herbicides that are rare in varieties, difficult to adapt to different ecological conditions, troublesome to use, and poor in effect. The species is prepared from fungal strains obtained through screening. It mainly controls crabgrass, and also removes weeds such as foxtail, barnyardgrass, and goosegrass. It is safe for crops, especially for monocotyledonous crops, and has wide application adaptability and low cost. Inexpensive, pollution-free biological herbicide; Another object of the present invention is to propose the preparation method of this biological herbicide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Embodiment 1: (screening of preventing and controlling crabgrass efficient strain Mds0404)

[0031] From the diseased plants of crabgrass crabgrass in the wild that naturally occurred in Yuyao, Zhejiang Province, the diseased leaves of crabgrass crabgrass were collected, and the pathogenic fungi were isolated and cultured with PDA medium. Several fungal strains were isolated from them, and their pathogenicity was tested. The following test: pick the single spores of each strain under the microscope, inoculate them on PDA medium respectively, and cultivate them in the dark at 28°C to observe the colony characteristics of the pure culture of pathogenic fungi; and inoculate each colony disc with a diameter of 6mm on the In the center of the PDA plate, measure the diameter of each colony after 7 days; prepare each strain to a concentration of 1×10 6 The spore suspension of each / ml is sprayed on 2 leaves and 1 heart crabgrass plant, handles after 4 days, observes the pathogen...

Embodiment 2

[0034] Embodiment 2: (measurement of bacterial strain Mds0404 host range)

[0035] Determination of the host range clearly shows that strain Mds0404 is safe to crops such as cotton, sunflower, soybean, cowpea, peanut, corn, wheat and rice; barnyardgrass, goosegrass and foxtail are infected, while crabgrass is a serious infection (see Table 2) , the control effect after one week is more than 85%.

[0036] Table 2 Determination of the host range of Curvularia crabgrass strain Mds0404

[0037] Branch name

[0038] Note: NS means no response; LS means infection; HS means serious infection

Embodiment 3

[0039] Embodiment 3: (a kind of biological herbicide of the present invention and preparation method 1 thereof)

[0040] Take 83 grams of pure spores of bacterial strain Mds0404, 15 grams of atrazine and 2 grams of Tween according to the formula, and mix them into 100 grams of biological herbicide. The preparation method of this agent is carried out according to the following steps:

[0041] (1) Preparation of culture medium: bacterial strain Mds0404 can be activated and induce the production of conidia with any of the following medium:

[0042] PDA medium: 150g potato, 10g glucose, 18g agar, 1L water;

[0043] PSA medium: 150g potato, 10g sucrose, 18g agar, 1L water;

[0044] Bran medium: 50g bran, 18g agar, 1L water;

[0045] Corn flour medium: corn flour 50g, agar 18g, water 1L;

[0046] Starch yeast medium: starch 10g, yeast powder 2g, agar 18g, water 1L;

[0047] Czapek medium: KNO 3 2g;K 2 HPO 4 1g; KCl 0.5g; MgSO 4 .7H 2 O 0.5g; FeSO 4 0.01g; 30g sucrose; 1...

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Abstract

The invention relates to a method for preparing biological weed killer, wherein it uses Curvularia Boedijn Mds0404 CGMCC NO. 1827 as seed, which comprises 80-95% spore, 0-18% chemical weed killer, and 2-5% agent. And its production comprises that: activating seed, cultivating seed liquid, cultivating spore and collecting components. The invention has low cost and wide application.

Description

technical field [0001] The present invention relates to a technical field of using microorganisms to control farmland weeds, in particular to a biological herbicide for controlling farmland weeds using fungal strains and a preparation method of the herbicide, which is specially used for farmland weeds crabgrass, goosegrass and Prevention and control of barnyardgrass, etc. Background technique [0002] With people's understanding of chemical herbicide residues, environmental pollution, and the development of drug-resistant and drug-resistant weeds, green food and organic agriculture, the development of biological herbicides to control weeds has become an urgent demand in production, and it has low investment. , short development cycle, strong specificity, environmental safety, no pollution and other advantages. At present, commercialized biological herbicide varieties include DeVine and College developed in the early 1980s and Comperico and Biochon launched in the late 1990s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P13/00A01N47/00A01N43/00A01N25/30
Inventor 杜新法邱海萍柴荣耀毛雪琴张震王艳丽王教瑜孙国昌
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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