20 results about "Bioherbicide" patented technology

The invention belongs to the technical field of agricultural biology, and discloses myrothecium verrucaria with herbicidal activity and application of the myrothecium verrucaria. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20251228. Experiments show that the bacterial strain has remarkable pathogenic activity on various weeds such as atrazine, ageratina adenophora, barnyard grass, amaranthus retroflexus and digitaria sanguinalis, the fatality rate of part of weeds under high spore concentration can reach 100%, and the maximum fresh weight inhibition rate can reach 87.47%. More importantly, the strain is safe to the growth of tomato crops and has no obvious adverse effect. The invention also provides application of the strain in preparation of microbial herbicides. The strain and the product thereof provide important strain resources and technical support for developing efficient, safe and environment-friendly novel biological herbicides, and are particularly suitable for green weed control in specific scenes such as tomato fields.

The invention relates to the technical field of orchard planting and soil fertilization management, and discloses a mango orchard ecological fertilization planting method with root zone annular drip covering and inter-row pasture mixed sowing, which comprises the following steps: field foundation pretreatment: field shallow rotation no-tillage, ecological ditch digging and biological herbicide spraying; paving a domain composite covering layer constructed by root domain composite covering and intelligent drip irrigation, clamping a drip irrigation ring, and controlling drip irrigation by a sensor; mixed sowing of the ternary pasture between the rows in the rainy season is conducted, and the ternary pasture is cut and utilized in good time; after composting the mushroom dregs, the mushroom dregs of the double-microbial-agent synergistic compost, the straws and the trimmed mango tree branches and leaves, adding double microbial agents of bacillus subtilis powder and trichoderma powder, and precisely fertilizing; and precise fertilization, ecological disease prevention and control and field maintenance: irrigating roots with trichoderma in a flowering period, and maintaining drip irrigation and a covering layer. According to the mango garden ecological fertilizing planting method with root domain annular dripping covering and inter-row pasture mixed sowing, the soil fertility can be improved, diseases can be prevented, and the quality and yield of mangoes can be improved.

The invention belongs to the technical field of biological herbicides, particularly discloses a sweet potato long-beak-shell bacterial strain Ans and application of volatile matter of the sweet potato long-beak-shell bacterial strain Ans, and provides a sweet potato long-beak-shell bacterial strain Ans, and the classification name of the sweet potato long-beak-shell bacterial strain Ans is as follows: sweet potato long-beak-shell bacterial strain Ans, and the classification name of the sweet potato long-beak-shell bacterial strain Ans is as follows: sweet potato long-beak-shell bacterial strain Ans. The preservation number is CGMCC No.41781. Volatile organic compounds of the sweet potato long-beak-shell bacteria Ans strain provided by the invention have a very good inhibition effect on germination of weed ageratum conyzoides seeds and are safe to germination of crop rice, VOCs components of the volatile organic compounds have diversity, n-propyl acetate, isobutyl acetate or isobutyl propionate can be developed into a microbial weed sealing agent in herbicides, and the application prospect is wide. The weeding composition is used for controlling weeds in crop planting fields, especially in crop planting fields with more weeds, namely ageratum conyzoides.

The invention belongs to the technical field of biological prevention and treatment, and particularly relates to a biological herbicide for preventing and treating solidago canadensis and a preparation and use method thereof.The biological herbicide is prepared from, by volume, 94%-97% of biological fermentation liquor, 0.75%-1% of Tween 80, 1.5%-2% of paraffin oil, 0.1%-0.2% of xanthan gum and 1%-2% of azone. The biological fermentation liquid is prepared by fermenting solidago canadensis. The preparation method comprises the following steps: S1, raw material treatment: selecting solidago canadensis as a plant raw material, cutting the solidago canadensis into pieces, performing high-pressure sterilization, and adding water for mixing to obtain a mixture; s2, adding the cultured bacillus subtilis and saccharomyces cerevisiae into the mixture in the step S1, mixing and inoculating, and stirring and fermenting to obtain a fermentation stock solution; s3, centrifuging the fermentation stock solution, taking supernate, filtering to obtain biological fermentation liquor, adding Tween 80, paraffin oil, xanthan gum and azone into the biological fermentation liquor at one time, stirring until the materials are completely dissolved, adjusting the pH value, and filtering to remove impurities to obtain the biological herbicide. The biological herbicide for preventing and treating the solidago canadensis can be used for preventing and treating seed germination, seedling growth and flowering processes of the solidago canadensis, and solves the problem that a biological medicament for preventing and treating the solidago canadensis is lacked at present.

The invention discloses a preparation method and application of a biological herbicide based on an RNA (Ribonucleic Acid) interference technology, which specifically comprises the following steps: designing a specific siRNA (Small Interference Ribonucleic Acid) sequence through a targeted glutamine synthetase gene (GS2), cloning a reverse repeat structure of the specific siRNA sequence to multiple cloning sites of an L4440 vector, and transforming the reverse repeat structure to RNase III defective escherichia coli HT115 for induced fermentation. By optimizing IPTG concentration and induction time, the thalli efficiently express target dsRNA, and a dsRNA aqueous solution is prepared after splitting decomposition and purification. Foliage spraying is adopted during spraying treatment, dsRNA can permeate into plant cells through pores, GS2 mRNA is specifically degraded, ammonium ions in the cells are accumulated, a photosynthetic system collapses, and leaves wither, wither and yellow and finally die within 72 hours. Compared with a traditional chemical herbicide, the herbicide has the advantages of being accurate in targeting, safe, environmentally friendly, free of residues and the like, and an efficient and sustainable biological solution is provided for agricultural weeding.

The present invention provides a composite fungal herbicide, belonging to the technical field of microbial herbicides. The composite fungal herbicide comprises active herbicidal ingredients: Streptomyces hygroscopicus M22 and Curvularia lunata B6. The combined use of Streptomyces hygroscopicus M22 and Curvularia lunata B6 creates a synergistic effect, broadens the weed control spectrum, and enhances the control of field weeds. The weed control effect is superior to that of a single agent, and is superior to other microbial herbicides. Furthermore, the composite herbicide is highly safe for crops and does not cause phytotoxicity.

The present invenon relates to the field of sustainable agriculture. More parcularly, the present disclosure provides composions and methods for natural pest management, more parcularly for inhibing or suppressing growth of unwanted plant species by making use of bioherbicides. The bioherbicide composions of current applicaon comprise one or more natural compounds produced by bacterial strains from the Streptomyces chrestomycecus species.

The invention discloses an isovanillin derivative as well as a preparation method and application thereof. The structural general formula of the isovanillin derivative is shown as a formula (II), and R1 to R5 are independently selected from any one of hydroxyl, alkyl, amino, sulfydryl, carboxyl, aldehyde group or alkoxy with 1-7 carbon atoms respectively. The isovanillin and the derivative thereof provided by the invention are wide in herbicide controlling spectrum and remarkable in effect. Meanwhile, the structure is simple, industrial production is easy, and the herbicide has the potential of being developed into biological herbicides. # imgabs0 #

This invention relates to the field of biocontrol bacteria technology, specifically to a type of barley brewing lees bacteria and its application in biological herbicides and fungicides, wherein the barley brewing lees bacteria is Bacillus subtilis (Bacillus subtilis). Bacillus subtle B9, whose 16S rDNA sequence is shown in SEQ ID NO:1, was deposited at the Guangdong Provincial Microbial Culture Collection Center on July 1, 2022, with accession number GDMCC No: 62595. The fermentation broth of *Bacillus oryzae* from this invention exhibits a 74.71% inhibitory effect on *Fusarium oxysporum* LM-C, and its inhibition rates on shoot length, root length, and fresh weight of *Rhizopus spp.* and *Oxalis spp.* seedlings all exceed 78%, making it suitable for preparing microbial herbicides.

The invention relates to the technical field of herbicides, in particular to a biological herbicide based on schizonepeta leaf water extract and a preparation method and application thereof. The invention discloses and proves that the schizonepeta leaf water extract has a remarkable and concentration-dependent allelopathy inhibition effect on seed germination and seedling growth of major weeds barnyard grass in farmland through systematic biological assay for the first time. The extract simultaneously and obviously inhibits the germination potential (germination speed), germination rate (germination ability), root length and bud length (early planting) of barnyard grass, realizes cooperative blocking of a plurality of key links in the early life stage of weeds, and has more thorough prevention and removal effects. The key concentration range (0.033 g / mL-0. 1 g / mL) and the optimal concentration (0.1 g / mL) of the schizonepeta leaf water extract for generating the remarkable inhibition effect are defined, and an accurate matching basis is provided for product development.

This invention discloses an extract of *Taxus chinensis* aril and its application. The extract is obtained through the following steps: 1) thoroughly crushing the *Taxus chinensis* aril using a mortar and pestle; 2) extracting with water and ethanol as solvents, followed by rotary evaporation to obtain the mother liquor; 3) diluting the mother liquor to the desired concentration. Experimental results show that treatment with a 0.3 g / mL concentration of the aqueous extract of *Taxus chinensis* aril can inhibit the growth of barnyard grass in rice paddies, providing a research basis for the development of biological herbicides. Therefore, this invention provides a feasible method for studying the allelopathic effects of *Taxus chinensis* aril on barnyard grass and rice, and has practical application value.

The present invention relates to bioherbicidal compositions comprising an extract from an insect of the genus Hermetia spp. and / or one or more fractions of this extract. The present invention further relates to uses of the extract or fractions of the extract as a bioherbicide, as well as corresponding methods for weed control.

The invention relates to the technical field of biology, in particular to a naphthoquinone derivative and a preparation method and application thereof. A series of novel naphthoquinone compounds are found from Fusarium sp.L-3 secondary metabolites for the first time, and the influence of treatment of the naphthoquinone derivatives on the growth of purslane roots, the influence of treatment of the naphthoquinone derivatives on the integrity of purslane root cell membranes, the weeding spectrum, the action mechanism and the toxicity of the naphthoquinone derivatives are studied. Results show that the naphthoquinone derivative can significantly inhibit growth of portulaca oleracea roots, destroy plasma membrane integrity and cause root cell death, plays a role mainly through a photosynthetic transmission chain plasma quinone competitive site, is safe and non-toxic, has the advantages of wide herbicide controlling spectrum, significant effect and unobvious toxicity, and meanwhile, has a simple structure, is easy for industrial production and has a broad application prospect. The bacillus subtilis has the potential of being developed into biological herbicides.

The present invention relates to bio-herbicide compositions comprising an extract from an insect of the genus Hermetia spp., and / or one or more fractions of this extract. The present invention further relates to uses of the extract or of the fractions of the extract as a bio-herbicide, and to corresponding methods for weed control.

This invention discloses a method for controlling Canadian goldenrod, belonging to the field of biological control technology. The method includes: physical tilling combined with chemical spraying during the seedling stage; tilling followed by application of biological herbicides during the vegetative growth stage; spraying flower bud inhibitors to suppress seed formation during the flowering stage; and physical removal during the seed maturation stage. This invention employs a phased, multi-pronged, synergistic comprehensive control strategy, targeting the biological characteristics of Canadian goldenrod at different growth stages, and combining chemical, biological, and physical methods to achieve highly efficient, long-lasting, and environmentally friendly control effects. This significantly reduces problems such as herbicide resistance, high recurrence rates, and environmental pollution caused by single-method control.

The invention relates to the technical field of biological control, in particular to a biological herbicide and a preparation method and application thereof. The biological herbicide is prepared from the following components in parts by mass: 4 to 8 parts of tribulus terrestris extract, 1 to 5 parts of horseweed herb extract, 1 to 5 parts of alternanthera philoxeroides extract, 4 to 8 parts of biological agent, 2 to 5 parts of surfactant, 2 to 5 parts of stabilizer, 0.2 to 1 part of synergist and 90 to 110 parts of water. The prevention and removal rate of broadleaf weeds and grassy weeds by using the biological herbicide disclosed by the invention is up to 90% or above, and compared with the existing chemical herbicide, the safety performance is improved, and the prevention and removal rate is also improved. A basis is provided for biological prevention and control of field weeds.

The application belongs to the field of biological weed control, and discloses a Calonectria ilicicola and application thereof in weed control. The Calonectria ilicicola is mainly used for the prevention and treatment of broadleaf weeds in agricultural production, and also shows pathogenic activity to some common grass weeds. The Calonectria ilicicola and metabolites thereof can cause diseases to various broadleaf weeds and some grass weeds, and have strong pathogenicity, so that target weeds such as Alternanthera philoxeroides and Ageratina adenophora can be effectively controlled. In addition, a metabolite 7α-hydroxytrichodermol is identified in the fermentation liquor of the fungus, and the metabolite also has pathogenic activity and can be used as an auxiliary or independent weed control active ingredient. The Calonectria ilicicola is safe to the environment, has certain selectivity and a wide herbicidal spectrum, and has the application potential of being developed into a biological herbicide.

The invention belongs to the technical field of biological herbicides, particularly discloses a sweet potato long-beak-shell bacterial strain S-2 and application of volatile matter of the sweet potato long-beak-shell bacterial strain S-2, and provides the sweet potato long-beak-shell bacterial strain S-2 which is classified and named as sweet potato long-beak-shell bacterial strain. The preservation number is CGMCC No.41782. Volatile organic compounds of the sweet potato long beak-shell fungus S-2 strain provided by the invention have a very good inhibition effect on germination of weed ageratum conyzoides seeds and are safe to germination of crop rice, and VOCs (Volatile Organic Compounds) components of the sweet potato long beak-shell fungus S-2 strain have diversity; wherein one or more of 2-methylbutyl acetate, myrcene and isoamyl acetate can be developed into a microbial weed sealing agent in herbicides, and the microbial weed sealing agent is used for preventing and controlling weeds in crop planting fields, especially for the crop planting fields with more weeds, namely ageratum conyzoides.

The invention belongs to the technical field of biological herbicides, particularly discloses a sweet potato long-beak-shell bacterial strain S-2 and application of volatile matter of the sweet potato long-beak-shell bacterial strain S-2, and provides the sweet potato long-beak-shell bacterial strain S-2 which is classified and named as sweet potato long-beak-shell bacterial strain. The preservation number is CGMCC No.41782. Volatile organic compounds of the sweet potato long beak-shell fungus S-2 strain provided by the invention have a very good inhibition effect on germination of weed ageratum conyzoides seeds and are safe to germination of crop rice, and VOCs (Volatile Organic Compounds) components of the sweet potato long beak-shell fungus S-2 strain have diversity; wherein one or more of 2-methylbutyl acetate, myrcene and isoamyl acetate can be developed into a microbial weed sealing agent in herbicides, and the microbial weed sealing agent is used for preventing and controlling weeds in crop planting fields, especially for the crop planting fields with more weeds, namely ageratum conyzoides.

This invention provides a strain MC-M1 for controlling Artemisia weeds, a biological herbicide, and its application, belonging to the field of Artemisia control technology. The gene sequence of strain MC-M1 is shown in SEQ ID NO: 1, and it was isolated from the leaves of naturally infected alfalfa. The fermentation broth of this strain showed a 100% inhibition rate on the germination of Artemisia seeds and an 83.33% disease incidence rate after spraying potted Artemisia. When its fermentation broth was mixed with a 1:1 volume ratio of bensulfuron-methyl solution at a bensulfuron-methyl concentration of 0.1165 g / L, the resulting compound microbial herbicide improved the control effect of Artemisia to 100%, significantly better than the single treatment, achieving synergistic effect. This provides a new strain resource and technical solution for the green control of Artemisia weeds, with good application prospects and ecological value.