Biological herbicide as well as preparation method and application thereof

Abstract

The invention relates to the technical field of biological control, in particular to a biological herbicide and a preparation method and application thereof. The biological herbicide is prepared from the following components in parts by mass: 4 to 8 parts of tribulus terrestris extract, 1 to 5 parts of horseweed herb extract, 1 to 5 parts of alternanthera philoxeroides extract, 4 to 8 parts of biological agent, 2 to 5 parts of surfactant, 2 to 5 parts of stabilizer, 0.2 to 1 part of synergist and 90 to 110 parts of water. The prevention and removal rate of broadleaf weeds and grassy weeds by using the biological herbicide disclosed by the invention is up to 90% or above, and compared with the existing chemical herbicide, the safety performance is improved, and the prevention and removal rate is also improved. A basis is provided for biological prevention and control of field weeds.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological control, and particularly relates to a biological herbicide, a preparation method and application thereof. BACKGROUND

[0002] Chemical herbicides are widely used in agricultural production, effectively solving the problem of competition for sheep manure, light and space between weeds and crops, and significantly improving the yield of crops. However, long-term use of chemical herbicides can cause a series of problems, such as soil residues of chemical agents, which can pollute groundwater and surface water through leaching, runoff and other ways, and damage the ecological environment; some chemical herbicides can enter the human body through the food chain, posing a threat to human health. At the same time, long-term use of a single chemical herbicide can also lead to increased weed resistance, resulting in a decrease in herbicidal effect year by year, thereby reducing crop yield.

[0003] Biological herbicides have become a hot topic in the field of herbicides due to their good environmental compatibility, difficulty in developing resistance, safety to crops and other advantages. Currently developed biological herbicides are mostly based on single bacterial agents or single plant-derived extracts. Single substances have problems such as low herbicidal efficiency and short persistence.

[0004] Therefore, a biological herbicide that can improve herbicidal efficiency and prolong persistence is urgently needed. SUMMARY

[0005] The present application aims to overcome the problems of single active ingredient, limited control effect and short persistence of existing biological herbicides. In particular, a biological herbicide, a preparation method and application thereof are provided.

[0006] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions:

[0007] The present application provides a biological herbicide, which comprises the following components in mass fraction:

[0008] 4-8 parts of Tribulus terrestris extract, 1-5 parts of Conyza canadensis extract, 1-5 parts of Alternanthera philoxeroides extract, 4-8 parts of biological bacterial agent, 2-5 parts of surfactant, 2-5 parts of stabilizer, 0.2-1 part of synergist, and 90-110 parts of water.

[0009] Preferably, the preparation method of the Tribulus terrestris extract is as follows:

[0010] Tribulus terrestris fruits are mixed with ethanol at a mass-volume ratio of 1g:8-12mL, reflux extraction is performed 1-3 times, the extraction liquid is combined, the extraction liquid is filtered, the filtrate is concentrated to 60℃ relative density of 1.2-1.4 to obtain an extract; and the extract is dried to obtain the Tribulus terrestris extract.

[0011] The initial concentration of the ethanol is 60-70vt%.

[0012] The temperature of the reflux extraction is 55-65℃;

[0013] The time of the reflux extraction is 1.5-2.5h / time;

[0014] The concentration is vacuum concentration; the vacuum degree of the vacuum concentration is 0.06-0.08MPa; the temperature of the vacuum concentration is 60-80℃;

[0015] The drying is spray drying; the inlet air temperature of the spray drying is 150-160℃; the outlet air temperature of the spray drying is 65-85℃; the tower pressure of the spray drying is -10--18Pa.

[0016] Preferably, the preparation method of the Potentilla indica extract is as follows:

[0017] Potentilla indica is mixed with ethanol at a mass-volume ratio of 1g:7-9mL, ultrasonic extraction is performed for 2-4 times, the extraction liquid is centrifuged, the supernatant is taken and concentrated to 1 / 4-1 / 3 of the original volume to obtain a concentrated liquid; the concentrated liquid is mixed with ethyl acetate, extraction is performed for 30-60min, the organic phase is taken, rotary evaporation and drying are performed to obtain the Potentilla indica extract;

[0018] The power of the ultrasonic extraction is 400-500W, and the time of the ultrasonic extraction is 30-40min / time;

[0019] The centrifugal speed is 10000-12000rpm, and the centrifugal time is 10-15min;

[0020] The volume ratio of the concentrated liquid to ethyl acetate is 1:1-3;

[0021] The extraction temperature is 50-60℃.

[0022] Preferably, the preparation method of the Alternanthera philoxeroides extract is as follows:

[0023] Alternanthera philoxeroides is mixed with methanol, reflux extraction is performed for 2-4 times, the extraction liquid is combined, filtration is performed, the supernatant is taken, and the supernatant is concentrated to 1 / 18-1 / 15 of the original volume to obtain a concentrated substance; the concentrated substance is mixed with diethyl ether for 3-5h, centrifugation is performed, the precipitate is collected, and drying is performed to obtain the Alternanthera philoxeroides extract;

[0024] The mass-volume ratio of the Alternanthera philoxeroides to methanol is 1g:10-14mL;

[0025] The initial concentration of the methanol is 65-75vt%;

[0026] The temperature of the reflux extraction is 50-60℃, and the time of the reflux extraction is 30-40min / time;

[0027] The volume ratio of the concentrate mixed with ethyl ether is 1:3-4.

[0028] Preferably, the biological agent comprises the following components in the following mass ratio:

[0029] Streptomyces microflavus: Bacillus amyloliquefaciens: Paecilomyces lilacinus is 1-3: 2-4: 1-3;

[0030] The initial viable bacterial concentration of the Streptomyces microflavus is 1-50 x 10 9 cfu / g;

[0031] The initial viable bacterial concentration of the Bacillus amyloliquefaciens is ≥ 1 x 10 11 cfu / g;

[0032] The initial viable bacterial concentration of the Paecilomyces lilacinus is 5-20 x 10 9 cfu / g.

[0033] Preferably, the surfactant is a mixture of Tween-80 and sodium dodecyl sulfonate in a mass ratio of 2-4:1;

[0034] The stabilizer comprises xanthan gum, trehalose and vitamin E in a mass ratio of 1-2:2-4:0.3-0.5;

[0035] The synergist is salicylic acid and / or humic acid;

[0036] When the synergist is salicylic acid and humic acid, the mass ratio of salicylic acid to humic acid is 1:3-5.

[0037] The present application also provides a preparation method of the biological herbicide, comprising the following steps:

[0038] (1) Take each component according to the mass fraction of the biological herbicide;

[0039] (2) Mix the Tribulus terrestris extract, the Matricaria maritima extract, the Alternanthera philoxeroides extract, the surfactant, the stabilizer and the synergist with water, and stir for 20-40 min to obtain a mixture;

[0040] (3) Mix the mixture with the biological agent, homogenize to obtain the biological herbicide.

[0041] Preferably, the stirring speed in step (2) is 80-100 rpm.

[0042] Preferably, the homogenization temperature in step (3) is 25-35℃, the homogenization pressure is 25-35 MPa, and the homogenization time is 1-2 h.

[0043] The application also provides application of the biological herbicide in inhibiting growth of weeds and increasing crop yield.

[0044] The weeds include broadleaf weeds and gramineous weeds.

[0045] Compared with the prior art, the application has the following beneficial effects:

[0046] (1) Significant herbicidal effect: the three plant source extracts Tribulus terrestris extract, Potentilla indica extract and Alternanthera philoxeroides extract respectively play roles by destroying weed cell membranes, interfering with weed respiratory metabolism and inhibiting weed root growth, and form a synergistic effect with biological fungicides, the control rate of the broadleaf weeds (Descurania sophia, Galium aparine, etc.) is ≥ 90%, and the control rate of the gramineous weeds (Echinochloa crus-galli, Digitaria sanguinalis, etc.) is ≥ 90%, which is higher than the control rate of a single active ingredient and the control rate of the existing chemical herbicides.

[0047] (2) Safety: through field tests in wheat and corn fields, the crop leaves using the biological herbicide of the application have no yellowing and wilting phenomenon, can promote crop root growth and increase crop yield.

[0048] (3) Environmental friendliness. DETAILED DESCRIPTION

[0049] Tribulus terrestris is a traditional medicinal plant, and the alkaloid extract thereof can destroy the integrity of weed cell membranes and inhibit the activity of key enzymes in photosynthesis; the flavonoid and terpenoid extracts of Potentilla indica can interfere with the respiration of weeds, leading to interruption of energy supply; and the saponin extract of Alternanthera philoxeroides can inhibit the growth of weed roots and block nutrient absorption. The three can effectively remove weeds in synergy.

[0050] The technical solutions provided by the application will be described in detail below in combination with the embodiments, but they should not be understood as limiting the protection scope of the application.

[0051] The Streptomyces microflavus in the embodiments of the application is purchased from Shandong Nuojie Biological Technology Co., Ltd., and the initial viable bacterial concentration is 1×10 9 cfu / g;

[0052] The Bacillus amyloliquefaciens and Paecilomyces lilacinus in the embodiments of the application are purchased from Shandong Ruigan Biological Technology Co., Ltd.; the initial viable bacterial concentration of the Bacillus amyloliquefaciens is 1×10 11 cfu / g; and the initial viable bacterial concentration of the Paecilomyces lilacinus is 5×10 9 cfu / g.

[0053] Embodiment 1

[0054] The Tribulus terrestris fruits were crushed to a particle size of 1 mm, 100 g of the crushed Tribulus terrestris fruits were mixed with 1000 mL of 65% by volume ethanol, and reflux extraction was performed twice at 65°C, each for 2 h, and the extract was combined. The extract was filtered through 5 layers of gauze, and the filtrate was concentrated to a paste with a relative density of 1.3 at 60°C in a vacuum concentration machine at a vacuum degree of 0.08 MPa and a temperature of 70°C. The extract was dried in a spray dryer at an inlet air temperature of 150°C, an outlet air temperature of 70°C, a tower pressure of -12 Pa, and a tower temperature of 80°C, until the water content was 6%, to obtain a Tribulus terrestris extract.

[0055] The Herba Conyzae canina was crushed to a particle size of 200 μm, 100 g of the crushed Herba Conyzae canina was mixed with 800 mL of 60% by volume ethanol, and ultrasonic extraction was performed three times at a power of 450 W, each for 35 min, and the extract was combined. The extract was centrifuged at 12000 rpm for 10 min, and the supernatant was collected. The supernatant was concentrated to 1 / 4 of the original volume by rotary evaporation at 60°C, 200 mL of ethyl acetate was added, and extraction was performed at 60°C for 50 min. The ethyl acetate organic phase was collected, and the rotary evaporation was performed at 60°C until it was semi-solid. The semi-solid was dried in a spray dryer at an inlet air temperature of 150°C, an outlet air temperature of 70°C, a tower pressure of -12 Pa, and a tower temperature of 80°C, until the water content was 6%, to obtain a Herba Conyzae canina extract.

[0056] The Alternanthera philoxeroides stems were crushed to a particle size of 200 μm, 100 g of the crushed Alternanthera philoxeroides was mixed with 1400 mL of 70% by volume methanol, and reflux extraction was performed three times at 55°C, each for 30 min, and the extract was combined. The extract was filtered through 5 layers of gauze, and the supernatant was collected. The supernatant was concentrated to 1 / 15 of the original volume by vacuum concentration, to obtain a concentrate. 300 mL of diethyl ether was added to the concentrate and mixed for 4 h, and the precipitate was collected by centrifugation at 10000 rpm for 15 min. The precipitate was dried in a spray dryer at an inlet air temperature of 150°C, an outlet air temperature of 70°C, a tower pressure of -12 Pa, and a tower temperature of 80°C, until the water content was 6%, to obtain an Alternanthera philoxeroides extract.

[0057] 20 g of Streptomyces microflavus, 30 g of Bacillus amyloliquefaciens, and 30 g of Paecilomyces lilacinus were mixed to obtain a biological microbial agent.

[0058] 30 g of Tween-80 and 10 g of sodium dodecyl sulfate were mixed to obtain a surfactant.

[0059] 15 g of xanthan gum, 20 g of trehalose, and 5 g of vitamin E were mixed to obtain a stabilizer.

[0060] 10 g of salicylic acid and 40 g of humic acid were mixed to obtain a synergist.

[0061] Take Tribulus terrestris extract 5g, Knapweed extract 5g, Alternanthera philoxeroides extract 1g, surfactant 2g, stabilizer 3g, synergist 0.8g and 100g water, stir at 90rpm for 30min to obtain a mixture. Add 6g biological fungicide to the mixture, homogenize at 30℃ and 30MPa for 2h to obtain a biological herbicide.

[0062] Example 2

[0063] Prepare Tribulus terrestris extract, Knapweed extract, Alternanthera philoxeroides extract, surfactant, stabilizer, synergist and biological fungicide according to the method of Example 1.

[0064] Take Tribulus terrestris extract 4g, Knapweed extract 3g, Alternanthera philoxeroides extract 3g, surfactant 3g, stabilizer 2g, synergist 0.2g and 90g water, stir at 80rpm for 40min to obtain a mixture. Add 4g biological fungicide to the mixture, homogenize at 25℃ and 30MPa for 2h to obtain a biological herbicide.

[0065] Example 3

[0066] Prepare Tribulus terrestris extract, Knapweed extract, Alternanthera philoxeroides extract, surfactant, stabilizer, synergist and biological fungicide according to the method of Example 1.

[0067] Take Tribulus terrestris extract 8g, Knapweed extract 1g, Alternanthera philoxeroides extract 5g, surfactant 5g, stabilizer 5g, synergist 1g and 110g water, stir at 100rpm for 20min to obtain a mixture. Add 8g biological fungicide to the mixture, homogenize at 35℃ and 30MPa for 2h to obtain a biological herbicide.

[0068] Comparative Example 1

[0069] Prepare the scheme of Comparative Example 1 according to the method of Example 1, except that no biological fungicide is added in Comparative Example 1, and the mixture is homogenized for 2h to obtain a biological herbicide.

[0070] Comparative Example 2

[0071] Prepare the scheme of Comparative Example 2 according to the method of Example 1, except that no plant extract is added in Comparative Example 2.

[0072] Comparative Example 3

[0073] Prepare the scheme of Comparative Example 3 according to the method of Example 1, except that the plant extract of Comparative Example 3 only adds Tribulus terrestris extract, and the amount of Tribulus terrestris extract is 11g, and the biological fungicide is removed.

[0074] Comparative Example 4

[0075] The scheme of Comparative Example 4 was set up according to the method of Example 1, except that the plant extract of Comparative Example 4 only added the extract of Knapweed, and the amount of the extract of Knapweed was 11 g, and the biological agent was removed.

[0076] Comparative Example 5

[0077] The scheme of Comparative Example 5 was set up according to the method of Example 1, except that the plant extract of Comparative Example 5 only added the extract of Alternanthera philoxeroides, and the amount of the extract of Alternanthera philoxeroides was 11 g, and the biological agent was removed.

[0078] Comparative Example 6

[0079] A herbicide, the herbicide was 200 g / L glufosinate ammonium aqueous agent.

[0080] Experimental Example 1

[0081] Barnyard grass, fleabane, speedwell, and crabgrass were respectively taken, and sowed in plastic pots, one kind of weed was sowed in each pot, and the seed number of the weed was 100 per pot, and each kind of weed was sowed in 30 pots, and when the weed grew to 3-leaf stage, each kind of weed was randomly divided into Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, Comparative Example 5 group, Comparative Example 6 group, and blank control group, 3 pots of each group of each kind of weed. Example 1-3 groups were respectively sprayed with the biological herbicides configured by Example 1-3 (50 times diluent), Comparative Example 1-5 groups were respectively sprayed with the herbicides configured by Comparative Example 1-5 (50 times diluent), and Comparative Example 6 group was sprayed with the herbicide of Comparative Example 6. The blank control group was sprayed with distilled water, 5 mL was sprayed per pot of each group, and the growth of each kind of weed was respectively counted at 24 h and 72 h after spraying, and the control rate was calculated. The diluent was diluted with water.

[0082] The control rate = (the number of weeds in the blank control group - the number of weeds in the treatment group) / the number of weeds in the blank control group x 100%. The results are shown in Tables 1-4.

[0083] Table 1 Effect of different herbicides on the control of barnyard grass

[0084]

[0085]

[0086] Table 2 Effect of different herbicides on the control of fleabane

[0087] Group 24h control rate % 72h control rate % Example 1 69.3 92.7 Example 2 70.4 95.2 Example 3 68.1 90.4 Comparative Example 1 47.6 62.3 Comparative Example 2 38.2 54.3 Comparative Example 3 16.4 22.8 Comparative Example 4 28.6 36.9 Comparative Example 5 18.3 24.5 Comparative Example 6 65.7 93.3

[0088] Table 3 Effect of different herbicides on the control of speedwell

[0089]

[0090]

[0091] Table 4 Control effect of different herbicides on Digitaria sanguinalis

[0092] Group 24h control rate % 72h control rate % Example 1 70.7 98.4 Example 2 71.3 95.6 Example 3 69.8 96.8 Comparative Example 1 34.6 42.6 Comparative Example 2 38.4 48.7 Comparative Example 3 16.9 25.6 Comparative Example 4 24.1 30.4 Comparative Example 5 18.2 25.8 Comparative Example 6 72.3 96.3

[0093] From Tables 1 to 4, it can be seen that the herbicides of Examples 1 to 3 have similar control rates on Echinochloa crus-galli, Gnaphalium affine, Galium aparine and Digitaria sanguinalis as glufosinate, but are significantly higher than those of Comparative Example 1 without bio-fermentation and Comparative Example 2 without plant extract, and are higher than those of Comparative Examples 3 to 5 with only one plant extract. This shows that the bio-herbicides prepared in Examples 1 to 3 can effectively control Echinochloa crus-galli, Gnaphalium affine, Galium aparine and Digitaria sanguinalis.

[0094] Experimental Example 2

[0095] Each of 100 seeds of Echinochloa crus-galli, Gnaphalium affine, Galium aparine and Digitaria sanguinalis was mixed and sowed in the field. A total of 10 groups were sowed, and each group had a sowed area of (2 m x 2 m). When the weeds of each group reached the 3-leaf stage, the weeds of Examples 1 to 3 were sprayed with the bio-herbicides prepared in Examples 1 to 3 (50 times dilution), the weeds of Comparative Examples 1 to 5 were sprayed with the herbicides prepared in Comparative Examples 1 to 5 (50 times dilution), and the weeds of Comparative Example 6 were sprayed with the herbicide of Comparative Example 6. The blank control group was sprayed with distilled water, and 20 mL was sprayed in each group. On the 5th day after spraying, the number of surviving weeds in each group was counted, and the control rate was calculated. The results are shown in Table 5. The dilution liquid can be diluted with water.

[0096] Control rate = (number of weeds in the blank control group - number of weeds remaining in each treatment group) / number of weeds in the blank control group x 100%.

[0097] Table 5 Control of different herbicides on weeds in field test

[0098]

[0099]

[0100] Table 5 shows that the bio-herbicides of Examples 1 to 3 have high control on broadleaf weeds and gramineous weeds, and are higher than the control effect of existing chemical herbicides, and are safer than existing chemical herbicides.

[0101] Experimental Example 3

[0102] A test was conducted in a wheat test field, and the wheat was Jimei 44. The wheat field was divided into three groups, i.e. Example 1 group, Comparative Example 6 group and blank control group.

[0103] When the wheat reached the 3-leaf stage, the Example 1 group was sprayed with the biological herbicide of Example 1 (50-fold dilution) at 3500 mL / acre; the Comparative Example 6 group was sprayed with the herbicide of Comparative Example 6 at 3500 mL / acre, and the blank control group was sprayed with an equal amount of distilled water. The spraying was repeated on the 30th day after the first spraying at the same dosage.

[0104] The weeds in the wheat fields of each group were observed on the 20th day after the second spraying, and the control rate was calculated. After the wheat matured, the wheat was harvested, the yield of the wheat in each field was calculated, and the growth of the wheat in each field was observed after the spraying. The results are shown in Table 6.

[0105] Control rate = (number of plants of a certain weed in the blank control group - number of plants of the certain weed remaining in each treatment group) / number of plants of the certain weed in the blank control group x 100%.

[0106] Table 6 Control of weeds in wheat fields by different herbicides

[0107] Group Barnyardgrass % Pigweed % Sheotherb % Crabgrass % Yield / kg / acre Example 1 98.5 92.7 95.2 92.7 602 Comparative Example 6 83.4 79.3 81.7 85.7 554

[0108] The yield of the wheat in the blank control group was 446 kg / acre.

[0109] On the 3rd day after each spraying, the leaves of the wheat sprayed with the herbicide of Comparative Example 6 turned yellow, and wilting occurred, and individual plants died. On the 15th day after each spraying, the leaves of the wheat in the Comparative Example 6 group turned green again.

[0110] Table 6 shows that the biological herbicide of the present application can effectively control broadleaf weeds and grass weeds in wheat fields, and is safer than chemical herbicides.

[0111] Experimental Example 4

[0112] The experiment was conducted in a corn field of Zhengdan 958 according to the method of Experimental Example 3. The control of broadleaf weeds and grass weeds in corn fields by different herbicides was studied. The results are shown in Table 7.

[0113] Table 7 Control in corn fields

[0114] Group Barnyardgrass % Pigweed % Sheotherb % Crabgrass % Yield / kg / acre Example 1 99.6 91.7 90.5 93.5 702 Comparative Example 6 89.4 80.6 82.3 86.3 654

[0115] The yield of the corn in the blank control group was 596 kg / acre.

[0116] Table 7 shows that the biological herbicide of the present application can effectively control weeds in corn fields.

[0117] From the above examples, the present application provides a kind of biological herbicide obtained by compounding three plant source extracts Tribulus terrestris extract, Herba Patriniae extract and Alternanthera philoxeroides extract with biological agent.The biological herbicide of the present application has a control rate of more than 90% for broadleaf weeds and gramineous weeds, which improves the safety performance and control rate compared with existing chemical herbicides, and provides a basis for biological control of field weeds.

[0118] The above only describes the preferred embodiments of the present application, and it should be noted that those skilled in the art can make some improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered within the scope of protection of the present application.

Claims

1. A bioherbicide, characterized in that, The components include the following mass parts: 4-8 parts of Tribulus terrestris extract, 1-5 parts of Knapweed extract, 1-5 parts of Alternanthera philoxeroides extract, 4-8 parts of biological agent, 2-5 parts of surfactant, 2-5 parts of stabilizer, 0.2-1 part of synergist, and 90-110 parts of water.

2. The bioherbicide of claim 1, wherein, The preparation method of the Tribulus terrestris extract is as follows: The Tribulus terrestris fruit is mixed with ethanol at a mass-volume ratio of 1 g:8-12 mL, and reflux extraction is performed for 1-3 times. The extraction liquid is combined and filtered. The filtrate is concentrated to a relative density of 1.2-1.4 at 60 DEG C to obtain an extract. The extract is dried to obtain the Tribulus terrestris extract. The initial concentration of the ethanol is 60-70 vol %. The temperature of the reflux extraction is 55-65 DEG C. The time of the reflux extraction is 1.5-2.5 h / time. The concentration is vacuum concentration. The vacuum degree of the vacuum concentration is 0.06-0.08 MPa. The temperature of the vacuum concentration is 60-80 DEG C. The drying is spray drying. The inlet air temperature of the spray drying is 150-160 DEG C. The outlet air temperature of the spray drying is 65-85 DEG C. The tower pressure of the spray drying is-10 to-18 Pa.

3. The bioherbicide of claim 2, wherein, The preparation method of the Knapweed extract is as follows: The Knapweed is mixed with ethanol at a mass-volume ratio of 1 g:7-9 mL, and ultrasonic extraction is performed for 2-4 times. The extraction liquid is centrifuged, and the supernatant is concentrated to 1 / 4-1 / 3 of the original volume to obtain a concentrated liquid. The concentrated liquid is mixed with ethyl acetate, and extraction is performed for 30-60 min. The organic phase is collected, and rotary evaporation and drying are performed to obtain the Knapweed extract. The power of the ultrasonic extraction is 400-500 W, and the time of the ultrasonic extraction is 30-40 min / time. The rotation speed of the centrifugation is 10 000-12 000 rpm, and the time of the centrifugation is 10-15 min. The volume ratio of the concentrated liquid to ethyl acetate is 1:1-3. The temperature of the extraction is 50-60 DEG C.

4. The bioherbicide of claim 1, wherein, The preparation method of the Alternanthera philoxeroides extract is as follows: The Alternanthera philoxeroides is mixed with methanol, and reflux extraction is performed for 2-4 times. The extraction liquid is combined and filtered. The supernatant is collected, and concentrated to 1 / 18-1 / 15 of the original volume to obtain a concentrated liquid. The concentrated liquid is mixed with diethyl ether for 3-5 h, centrifuged, and dried to obtain the Alternanthera philoxeroides extract. The mass-volume ratio of the Alternanthera philoxeroides to methanol is 1 g:10-14 mL. The initial concentration of the methanol is 65-75 vol %. The temperature of the reflux extraction is 50-60 DEG C, and the time of the reflux extraction is 30-40 min / time. The volume ratio of the concentrated liquid to diethyl ether is 1:3-4.

5. The bioherbicide of claim 1, wherein, The biological agent includes the following components in the following mass ratio: Streptomyces microflavus, Bacillus amyloliquefaciens, and Paecilomyces lilacinus are in a mass ratio of 1-3:2-4:1-3. The initial viable cell concentration of the Streptomyces microflavus is 1-50 x 10 9 cfu / g; The initial viable cell concentration of the Bacillus amyloliquefaciens is ≥ 1 x 10 11 cfu / g; The initial viable cell concentration of the said P. lilacinum is 5-20 x 10 9 cfu / g.

6. The bioherbicide of claim 1, wherein, The surfactant is obtained by mixing Tween-80 and sodium dodecyl sulfonate at a mass ratio of 2-4:

1. The stabilizer includes xanthan gum, trehalose, and vitamin E in a mass ratio of 1-2:2-4:0.3-0.

5. The synergist is salicylic acid and / or humic acid. When the synergist is salicylic acid and humic acid, the mass ratio of salicylic acid to humic acid is 1:3-5.

7. A method for the production of a bioherbicide, characterized by, The method comprises the following steps: (1) taking the components of the biological herbicide according to any one of claims 1-6 by mass fraction; (2) mixing the Tribulus terrestris extract, the Gnaphalium affine extract, the Alternanthera philoxeroides extract, the surfactant, the stabilizer and the synergist with water, stirring for 20-40 min to obtain a mixture; (3) mixing the mixture with the biological agent, homogenizing to obtain the biological herbicide.

8. The preparation method according to claim 7, characterized in that, The stirring speed in step (2) is 80-100 rpm.

9. The production method according to claim 8, characterized by, The homogenizing temperature in step (3) is 25-35°C, the homogenizing pressure is 25-35 MPa, and the homogenizing time is 1-2 h.

10. The biological herbicide according to any one of claims 1-6 is used for inhibiting the growth of weeds and improving the yield of crops. The weeds include broadleaf weeds and grass weeds.

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