Colletotrichum Corda strain and biological herbicide containing spores thereof
A herbicide and anthracnose technology, applied in the direction of biocides, herbicides, algicides, biocides, etc., can solve the problems of increasing the difficulty of production, unsatisfactory single-strain control effect, and unmentioned safety. Achieve the effects of low cost, high spore yield and easy access to raw materials
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Embodiment 1
[0032] Embodiment 1: Screening of Cds0501 efficient strain Cds0501 for controlling crabgrass
[0033] From the diseased plants of crabgrass in the wild in Xinchang, Zhejiang Province, the diseased leaves of crabgrass were collected, and the pathogenic fungi were isolated and cultured with PDA medium. Several fungal strains were isolated from them, and their pathogenicity was tested. The following test: pick the single spores of each strain under the microscope, inoculate them on PDA medium respectively, and cultivate them in the dark at 28°C to observe the colony characteristics of the pure culture of pathogenic fungi; and inoculate each colony disc with a diameter of 6mm on the In the center of the PDA plate, measure the diameter of each colony after 7 days; prepare each strain to a concentration of 1×10 6 The spore suspension of each / mL is sprayed on 2 leaves and 1 heart crabgrass plant, and after 4 days of treatment, observe the pathogenic characteristics (see table 1) of e...
Embodiment 2
[0036] Embodiment 2: Determination of the host range of bacterial strain Mds0404
[0037] It is clear that the strain Cds0501 is safe to crops such as cotton, sunflower, soybean, cowpea, peanut, corn, wheat and rice through the determination of the host range; barnyardgrass, goosegrass and foxtail are infected, while crabgrass is seriously infected (see Table 2) , the control effect after one week is about 65%.
[0038] Table 2 Determination of the host range of C. crabgrass anthracnose strain Cds0501
[0039]
[0040]
[0041] Note: NS means no response; LS means infection; HS means severe infection
Embodiment 3
[0042] Embodiment 3: a kind of biological herbicide of the present invention and its preparation method 1
[0043] Take 83 grams of pure spores of bacterial strain Cds0501, 15 grams of atrazine and 2 grams of Tween according to the formula, and mix them into 100 grams of biological herbicide. The preparation method of this agent is carried out according to the following steps:
[0044] (1) Preparation of culture medium: strain Cds0501 can be activated and induce the production of conidia with any of the following medium:
[0045] PDA medium: 150g potato, 10g glucose, 18g agar, 1L water;
[0046] PSA medium: 150g potato, 10g sucrose, 18g agar, 1L water;
[0047] Bran medium: 50g bran, 18g agar, 1L water;
[0048] Corn flour medium: corn flour 50g, agar 18g, water 1L;
[0049] Starch yeast medium: starch 10g, yeast powder 2g, agar 18g, water 1L;
[0050] Czapek medium: KNO 3 2g;K 2 HPO 4 1g; KCl 0.5g; MgSO 4 .7H 2 O 0.5g; FeSO 4 0.01g; 30g sucrose; 18g agar; 1L wat...
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