Colletotrichum Corda strain and biological herbicide containing spores thereof

A herbicide and anthracnose technology, applied in the direction of biocides, herbicides, algicides, biocides, etc., can solve the problems of increasing the difficulty of production, unsatisfactory single-strain control effect, and unmentioned safety. Achieve the effects of low cost, high spore yield and easy access to raw materials

Inactive Publication Date: 2012-07-04
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the patent document proposed its use technology and production method, it only made it clear that it was safer for soybeans and cotton, but did not mention the safety and use of monocot crops such as corn and rice.
In addition, domestic Nanjing Agricultural University has applied for a patent (ZL patent No. 01134002.9) for biological control of crabgrass by using Nakataea Hara strain QZ-2000 (preservation number: CGMCC NO.0640), but its single-strain control effect Not ideal, the spores of several individual plants need to be mixed in proportion and then sprayed to achieve a better control effect, which increases the difficulty of production

Method used

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  • Colletotrichum Corda strain and biological herbicide containing spores thereof
  • Colletotrichum Corda strain and biological herbicide containing spores thereof
  • Colletotrichum Corda strain and biological herbicide containing spores thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Screening of Cds0501 efficient strain Cds0501 for controlling crabgrass

[0033] From the diseased plants of crabgrass in the wild in Xinchang, Zhejiang Province, the diseased leaves of crabgrass were collected, and the pathogenic fungi were isolated and cultured with PDA medium. Several fungal strains were isolated from them, and their pathogenicity was tested. The following test: pick the single spores of each strain under the microscope, inoculate them on PDA medium respectively, and cultivate them in the dark at 28°C to observe the colony characteristics of the pure culture of pathogenic fungi; and inoculate each colony disc with a diameter of 6mm on the In the center of the PDA plate, measure the diameter of each colony after 7 days; prepare each strain to a concentration of 1×10 6 The spore suspension of each / mL is sprayed on 2 leaves and 1 heart crabgrass plant, and after 4 days of treatment, observe the pathogenic characteristics (see table 1) of e...

Embodiment 2

[0036] Embodiment 2: Determination of the host range of bacterial strain Mds0404

[0037] It is clear that the strain Cds0501 is safe to crops such as cotton, sunflower, soybean, cowpea, peanut, corn, wheat and rice through the determination of the host range; barnyardgrass, goosegrass and foxtail are infected, while crabgrass is seriously infected (see Table 2) , the control effect after one week is about 65%.

[0038] Table 2 Determination of the host range of C. crabgrass anthracnose strain Cds0501

[0039]

[0040]

[0041] Note: NS means no response; LS means infection; HS means severe infection

Embodiment 3

[0042] Embodiment 3: a kind of biological herbicide of the present invention and its preparation method 1

[0043] Take 83 grams of pure spores of bacterial strain Cds0501, 15 grams of atrazine and 2 grams of Tween according to the formula, and mix them into 100 grams of biological herbicide. The preparation method of this agent is carried out according to the following steps:

[0044] (1) Preparation of culture medium: strain Cds0501 can be activated and induce the production of conidia with any of the following medium:

[0045] PDA medium: 150g potato, 10g glucose, 18g agar, 1L water;

[0046] PSA medium: 150g potato, 10g sucrose, 18g agar, 1L water;

[0047] Bran medium: 50g bran, 18g agar, 1L water;

[0048] Corn flour medium: corn flour 50g, agar 18g, water 1L;

[0049] Starch yeast medium: starch 10g, yeast powder 2g, agar 18g, water 1L;

[0050] Czapek medium: KNO 3 2g;K 2 HPO 4 1g; KCl 0.5g; MgSO 4 .7H 2 O 0.5g; FeSO 4 0.01g; 30g sucrose; 18g agar; 1L wat...

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Abstract

The invention provides a biological herbicide containing spores produced by a Colletotrichum Corda strain with a collection number of CGMCC No.2867, and a method for preparing the biological herbicide and a method for using the herbicide to remove weeds from crops. The invention particularly provides a biological herbicide for removing digitaria sanguinalis from crops, which comprises the spores produced by the Colletotrichum Corda strain with the collection number of CGMCC No.2867. The biological herbicide can prevent and control weeds in dicotyledons, and prevent and control weeds in monocotyledons; and the strain particularly has 85 to 100 percent of control effect on a target weed digitaria sanguinalis, particularly on plants with less than 3 leaves.

Description

technical field [0001] The invention relates to a fungus belonging to the genus Anthracnose and a biological weeding method thereof, which belongs to the technical field of microorganisms used for agricultural plant protection and crop weed control, and is specially used for the control of farmland weeds. Background technique [0002] With people's understanding of chemical herbicide residues, environmental pollution, and the development of drug-resistant and drug-resistant weeds, green food and organic agriculture, the development of biological herbicides to control weeds has become an urgent demand in production, and it has low investment. , short development cycle, strong specificity, environmental safety, no pollution and other advantages. At present, commercialized biological herbicide varieties include DeVine and College developed in the early 1980s and Comperico and Biochon launched in the late 1990s. These herbicides play an important role in controlling relative ta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01N43/70A01N37/26A01N43/60A01N43/40A01P13/00C12R1/645
Inventor 邱海萍王艳丽柴荣耀毛学琴张震姜华王教瑜杜新法孙国昌
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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