Cockroach glycoprotein as well as preparation method and application thereof

A technology of cockroach sugar and glycoprotein, applied in the field of medicine, to achieve the effect of delaying the occurrence and development

Active Publication Date: 2021-11-30
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The extraction and purification of cockroach glycoprotein from cockroach medicinal materials and the research on the immune regulation and antioxidation of cockroach glycoprotein have not been reported yet.

Method used

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  • Cockroach glycoprotein as well as preparation method and application thereof
  • Cockroach glycoprotein as well as preparation method and application thereof
  • Cockroach glycoprotein as well as preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0038] The invention discloses a preparation method of cockroach glycoprotein, comprising the following steps:

[0039] Step 1. Raw material processing: Raise the temperature of the cockroach adult breeding workshop to 40-50°C and maintain it for 20-30 minutes to kill, or pour the adults into warm water at about 40-50°C to kill, and separate the impurities in the adults Sieve out, clean the cockroach with warm water, dry with hot air below 80°C until the water content is less than 5%, and obtain the medicinal material, crush it, and set aside;

[0040] Step 2, the extraction of cockroach glycoprotein:

[0041] Step 2.1, put the pulverized medicinal material in an extraction tank, add water according to the ratio of material to liquid (g / ml) of 1:7~1:9, stir, heat and reflux at 80~90°C for 2~3 hours, filter, collect the filtrate, Repeated extraction 3 to 4 times;

[0042] Step 2.2, Concentration: Concentrate the obtained filtrate under reduced pressure at 60-70°C to 1 / 5-2 / 5 o...

Embodiment 1

[0052] A preparation method of cockroach glycoprotein, comprising the following steps:

[0053] Step 1. Raw material processing: Raise the temperature of the cockroach adult breeding workshop to 40-50°C and maintain it for 20-30 minutes to kill, or pour the adults into warm water at about 40-50°C to kill, and separate the impurities in the adults Sieve out, clean the cockroach with warm water, dry with hot air below 80°C until the water content is less than 5%, and obtain the medicinal material, crush it, and set aside;

[0054] Step 2, the extraction of cockroach glycoprotein:

[0055] Step 2.1. Weigh 100g of the pulverized medicinal material, put it in the extraction tank, add water according to the ratio of material to liquid (g / ml) of 1:9, stir, heat and reflux at 90°C for 2.25 hours, filter, collect the filtrate, and extract repeatedly for 4 Second-rate;

[0056] Step 2.2, Concentration: Concentrate the obtained filtrate under reduced pressure at 65°C to 1.5 / 5 of the or...

Embodiment 2

[0063] A preparation method of cockroach glycoprotein, comprising the following steps:

[0064] Step 1. Raw material processing: Raise the temperature of the cockroach adult breeding workshop to 40°C for 30 minutes to kill, or pour the adults into warm water at about 40°C to kill, and sieve out the impurities in the adults. Wash it with warm water, dry it with hot air below 80°C until the moisture content is less than 5%, obtain the medicinal material, crush it, and set aside;

[0065] Step 2, the extraction of cockroach glycoprotein:

[0066] Step 2.1, put the pulverized medicinal material in an extraction tank, add water according to the ratio of material to liquid (g / ml) of 1:7, stir, heat and reflux at 80°C for 3 hours, filter, collect the filtrate, and repeat the extraction 3 times;

[0067] Step 2.2, concentration: Concentrate the obtained filtrate under reduced pressure at 60°C to 2 / 5 of the original volume to obtain a concentrated solution with a specific gravity of 1...

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Abstract

The invention discloses cockroach glycoprotein as well as a preparation method and application thereof. The preparation method comprises the following steps: raw material treatment: killing cockroach adults, screening out impurities in the adults, cleaning the cockroaches with warm water, drying to obtain medicinal materials, and crushing for later use; performing extraction of cockroach glycoprotein; and purifying the cockroach glycoprotein. According to the invention, a glycoprotein substance for regulating immunocompetence and antioxidant activity is extracted from cockroaches, and the glycoprotein substance is an animal-derived natural immunomodulator and antioxidant and can be applied to treatment of immunosuppression, immune disorder and chronic diseases related to lipid peroxidation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a cockroach glycoprotein and its preparation method and application. Background technique [0002] Glycoproteins are a type of glycosylated binding protein formed by covalently linking oligosaccharides and polypeptides or proteins. Its structure mainly includes two parts: polypeptide chains and sugars, which are linked by covalent bonds. As a class of binding proteins, glycoproteins widely exist in various forms in animals, plants and microorganisms, and play roles in organisms such as cell signal recognition, growth regulation, intercellular information transmission and immune activity regulation. Studies have shown that glycoprotein has the functions of enhancing immune regulation, inhibiting tumors, lowering blood sugar, lowering blood lipids, anti-oxidation, and anti-aging. [0003] Cockroach (Periplaneta americana L.) is one of the insects of the genus Periplan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/30C07K1/34A61K35/64A61K38/01A61P37/02A61P37/04A61P39/06
CPCC07K1/145C07K1/34C07K1/30C07K1/14A61K38/012A61K35/64A61P37/02A61P37/04A61P39/06Y02A50/30
Inventor 杨永寿肖培云郝芳芳何正春顾婷和英陈怡袁丽萍王宝宇周永芳
Owner DALI UNIV
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