Immunochromatographic test strip for rapid detection of ancylostomiasis infection and preparation method thereof

An immunochromatography and hookworm disease technology, which is applied in the field of bioengineering, can solve the problems of high requirements for instruments, equipment and operators, and is not suitable for on-site use, and achieves the effects of easy industrial production, low cost, and simple detection methods.

Inactive Publication Date: 2015-11-11
STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of immunochromatographic test strip and preparation method thereof for rapid detection of hookworm infection, and the described immunochromatographic test strip and prepara...

Method used

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  • Immunochromatographic test strip for rapid detection of ancylostomiasis infection and preparation method thereof
  • Immunochromatographic test strip for rapid detection of ancylostomiasis infection and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of the immunochromatographic test strip that detects hookworm infection

[0039] 1. Preparation of purified antigen against hookworm

[0040] About 2000 adult hookworms of American hookworm were collected, washed repeatedly with phosphate buffer, and the worms were placed in a pre-cooled mortar and continuously poured into liquid nitrogen for thorough grinding. Then add 20 times the volume of worm body volume containing 1.5% n-octyl glucoside, 1mM phenylmethylsulfonyl fluoride, 0.2mM tosyllysyl chloromethanone, 1mM ethylenediaminetetraacetic acid, 1mM ethylene glycol bismuth (2-Aminoethyl ether) tetraacetic acid soaked and rinsed the collected parasite material, repeated freezing and thawing 5 times in liquid nitrogen and 37°C, and ice-bathed overnight. Centrifuge at 26000g at 4°C for 30 minutes, and dialyze the supernatant against phosphate buffer for 48 hours. Ammonium sulfate was added to the solution to 70% saturation and a precipitat...

Embodiment 2

[0061] Embodiment 2, the preparation of the immunochromatographic test strip that detects hookworm infection

[0062] Colloidal gold-labeled detection probes were prepared from Staphylococcus aureus protein A (SPA) purchased from Shanghai Yeli Biotechnology Co., Ltd., and the quality control line was coated with chicken anti-Staphylococcus aureus protein A IgY purchased from Jiening Biotechnology Co., Ltd. , other steps are with embodiment 1.

Embodiment 3

[0063] Embodiment 3, laboratory assessment of the immunochromatographic test strip for detecting hookworm infection

[0064] 1. Detection method

[0065] Add the serum to be tested to the blood filtration membrane sample pad of the immunochromatography test strip prepared in Example 1, add 1 drop (about 50 μL) of sample diluent (PBS) after 1 minute, start to observe the results after 2 minutes, and stop after 20 minutes . Judgment of results: If red bands appear on both test line 5 and quality control line 6, it is judged as hookworm disease; if only red bands appear on quality control line 6, it is judged as negative.

[0066] 2. Experimental results

[0067] Table 1 shows the results of laboratory assessment using the immunochromatographic immunochromatographic test strip for detecting hookworm infection prepared in Example 2 of the present invention. It can be seen from Table 1 that the sensitivity of the immunochromatographic test paper of the present invention can reac...

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Abstract

The invention relates to an immunochromatographic test strip for rapid detection of ancylostomiasis infection. The test strip includes: a sample pad, a gold labeled pad that is closely connected to the sample pad and contains a colloidal gold labeled probe, a cellulose membrane closely connected to the gold labeled pad and a water absorption pad closely connected to the other end of the cellulose membrane. One cellulose membrane end far from the gold labeled pad is provided with a quality control line, the cellulose membrane between the quality control line and the gold labeled pad is equipped with a detection line, which is composed of a hookworm purification worm antigen, and the hookworm purification adult worm antigen is a soluble antigen solution of Necator Americanus adult worms. The colloidal gold labeled probe is staphylococcus aureus A protein or s streptococcus G protein. The control line is composed of the antibody specifically binding with the colloidal gold labeled probe. The invention also provides a preparation method of the immunochromatographic test strip. The immunochromatographic test strip provided by the invention has the advantages of simplicity, sensitivity, specificity and rapidity, and can be applied to clinical and field use of human, livestock and wild animals at the same time.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a hookworm disease, in particular to an immunochromatographic test strip for rapidly detecting hookworm infection and a preparation method thereof. Background technique [0002] Hookworm disease (Ancylostomasis) includes duodenal hookworm disease and American hookworm disease, which are caused by Ancylostoma Duodenale and Necator Americanus parasitizing the human body respectively. Clinically, hookworm disease can be divided into the skin (mucosa) invasion stage, the hookworm lung migration stage and the adult intestinal parasitic stage. Hookworm infection can not only lead to malnutrition, anemia, growth retardation, intellectual impairment, etc. in children, which seriously endangers the growth and development of children, but also can cause anemia, malnutrition, gastrointestinal dysfunction, and decreased labor capacity in adults, especially pregnant women. Cause dev...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/532
CPCG01N33/532G01N33/558G01N2333/43526
Inventor 汪俊云石锋杨玥涛高春花杨益朱慧慧陈颖丹
Owner STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
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