Schistosoma japonicum gene recombination living vaccine production technique taking porcine pseudorabies virus as vector and product

A porcine pseudorabies and recombinant vaccine technology, applied in the field of production technology and products of Schistosoma japonicum gene recombinant live vaccine, can solve the problems of large schistosomiasis, unsatisfactory immune protection effect, complex antigen, etc., and achieve long shelf life and good immunity Protective effect, non-toxic side effects

Inactive Publication Date: 2009-06-03
MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Schistosoma japonicum has large body and complex antigens, so far, the immune protection effect induced by a single genetically engineered vaccine is not ideal

Method used

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  • Schistosoma japonicum gene recombination living vaccine production technique taking porcine pseudorabies virus as vector and product
  • Schistosoma japonicum gene recombination living vaccine production technique taking porcine pseudorabies virus as vector and product
  • Schistosoma japonicum gene recombination living vaccine production technique taking porcine pseudorabies virus as vector and product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of Live Vaccine with Recombinant Schistosoma japonicum Sj26GST Gene Pseudorabies Virus Vector

[0032] According to attached figure 1 The process route shown is to prepare the recombinant Schistosoma japonicum Sj26GST gene pseudorabies virus vector live vaccine. Specific steps are as follows:

[0033] 1. Cloning of Schistosoma japonicum protective antigen Sj26GST gene and construction of eukaryotic expression vector

[0034] Rabbits were infected with 1500 cercariae of Schistosoma japonicum and dissected after 45 days. Perfuse the portal vein with sterile sodium citrate saline, collect adult worms, and wash with sterile saline to remove blood components attached to the surface of the worms. Take 0.5 g of fresh adults, homogenate on ice, and use the Trizol method to prepare adult mRNA. The protective antigen Sj26GST was obtained by RT-PCR. After the amplified product was confirmed by agarose gel electrophoresis, the obtained gene was cloned into the pMD-...

Embodiment 2

[0054] Preparation of Live Vaccine with Recombinant Schistosoma japonicum Sj23 Gene Pseudorabies Virus Vector

[0055] According to attached figure 1 The process route shown is to prepare the recombinant Schistosoma japonicum Sj23 gene pseudorabies virus vector live vaccine. Specific steps are as follows:

[0056] 1. Cloning of Schistosoma japonicum protective antigen Sj23 gene and construction of eukaryotic expression vector

[0057] Rabbits were infected with 1500 cercariae of Schistosoma japonicum and dissected after 45 days. Perfuse the portal vein with sterile sodium citrate saline, collect adult worms, and wash with sterile saline to remove blood components attached to the surface of the worms. Take 0.5 g of fresh adults, homogenate on ice, and use the Trizol method to prepare adult mRNA. The protective antigen Sj23 was obtained by RT-PCR. After the amplified product was confirmed by agarose gel electrophoresis, the obtained gene was cloned into the pMD-18T vector t...

Embodiment 3

[0077] Preparation of Live Vaccine with Recombinant Schistosoma japonicum FABP Gene and Pseudorabies Virus Vector

[0078] According to attached figure 1 The shown process route prepares the recombinant schistosoma japonicum FABP gene pseudorabies virus vector live vaccine. Specific steps are as follows:

[0079] 1. Cloning of Schistosoma japonicum protective antigen FABP gene and construction of eukaryotic expression vector

[0080] Rabbits were infected with 1500 cercariae of Schistosoma japonicum and dissected after 45 days. Perfuse the portal vein with sterile sodium citrate saline, collect adult worms, and wash with sterile saline to remove blood components attached to the surface of the worm body. Take 0.5 g of fresh adults, homogenate on ice, and use the Trizol method to prepare adult mRNA. The protective antigen FABP was obtained by RT-PCR. After the amplified product was confirmed by agarose gel electrophoresis, the obtained gene was cloned into the pMD-18T vector ...

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Abstract

The invention provides a Schistosoma japonicum gene recombination living vector vaccine which is composed of porcine pseudorabies and a schistosoma japonicum antigen gene; the schistosoma japonicum antigen gene comprises Sj26GST, SjFABP and Sj23 to form monovalent recombination living vector vaccines rPRV/Sj26GST, rPRV/FABP and rPRV/Sj23 as well as multivalent living vector vaccines rPRV/Sj26GST-FABP and rPRV/Sj26GST-FABP-Sj23; the imago reduction rate is between 38.4 and 56.5 percent; the liver egg reduction rate is between 38.8 and 70.6 percent; the vaccine can effectively induce the immunity protection force for resisting the schistosoma japonicum, can generate longer immunity protection effect for one time immunity, does not have pollution or harmful effect to environment and does not cause untoward effect.

Description

technical field [0001] The invention provides a production process and product of a schistosomiasis gene recombinant live vaccine with porcine pseudorabies virus as a carrier, which is used for the prevention and treatment of domestic animal (cattle, sheep) schistosomiasis, and belongs to the technical field of animal infectious disease vaccine preparation. Background technique [0002] Schistosomiasis japonicum is a zoonotic parasitic disease that seriously endangers human health and affects economic and social development. In addition to infecting humans, it can also infect livestock such as cattle and sheep, causing significant economic losses to animal husbandry. Drug treatment alone cannot solve the problem of repeated infection of humans and animals. Therefore, the development of animal schistosomiasis vaccine is an important basis and technical guarantee for the control and elimination of schistosomiasis. [0003] With the development of modern molecular biology and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K48/00C12N15/12C12N15/86C12N7/01A61P33/12
CPCY02A50/30
Inventor 刘全商立民扈荣良朱兴全夏志平袁子国
Owner MILITARY VETERINARY RES INST PLA MILITARY MEDICAL ACAD OF SCI
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