Wheat interspecific distant hybrid, secondary backcross and selfing breeding method
A technology of distant hybridization and hybridization, which is applied in the field of crop genetics and breeding research, can solve the problems of failure to break through the thousand-grain weight of wheat germplasm and increase the production cost of pharmaceutical purchase, and achieve the effect of low production cost and strong genetic stability
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[0020] a. Parent selection: common wheat (T.aestivum) variety "Shi 4185" (genome AABBDD) and durum wheat (T.durum) variety "Neixiang 4184" (genome AABB) with a large genetic background were selected as parents ;
[0021] b. Hybridization: the common wheat (T. aestivum) variety "Shi 4185" was used as the female parent, and the durum wheat (T.durum) variety "Neixiang 4184" was used as the male parent to prepare a hybrid combination. Carry out artificial detasseling of 230 florets on the second day after the heading of the female parent, bagging and isolation after emasculation; pollination at 8-9 am and 15-16 pm on the second day after the female parent blooms, repeat three times in a row (days) . After pollination, they were still bagged for isolation. 15 days after pollination, the hybrid ear bag was removed to ensure that the hybrid grains were well developed, and the interspecific hybrid F 0 56 seeds;
[0022] C. Fertility identification: the seeds of the hybrid combinat...
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