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Tissue culture and rapid propagation method for campanumoea

A technology of tissue culture rapid propagation and soil Codonopsis pilosula, applied in horticultural methods, soilless cultivation, botanical equipment and methods, etc., can solve the problems of uneven quality of seedlings, difficulty in meeting high-quality seedlings, and lack of natural resources. To achieve the effect of strong pertinence, feasible operation, and expansion of sources

Inactive Publication Date: 2010-10-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The medicinal plant Codonopsis pilosula is seldom cultivated. The traditional Chinese medicinal material Codonopsis pilosula is collected wildly, and its growth is relatively slow, which makes this natural resource increasingly scarce. Under natural conditions, seeds or rhizomes are used for reproduction, but the germination rate of its seeds is low and the reproduction rate is low. , the quality of seedlings is uneven, and it is difficult to meet the requirements for high-quality seedlings in production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1), the selection and pretreatment of explants: cut the newly germinated shoots with a length of 5-10 cm, cut off the leaves, rinse with tap water with 1% (volume ratio) detergent for 10-30 minutes , Rinse with tap water for 20-40 minutes;

[0031] 2), explant sterilization: cut the pretreated twigs into 1-2.cm long stem sections with terminal buds or stem sections with axillary buds as explants, and use them for later use after surface sterilization;

[0032] 3), induction culture: under aseptic conditions, after the surface-sterilized stem section with apical bud or the stem section with axillary bud explant, one end or two ends of the incision browning are removed, and the apical bud and the stem section with axillary bud are removed. The buds are inoculated upwards in the induction medium, and after 30-45 days of cultivation under culture conditions, the terminal buds and axillary buds are induced to germinate, and then clustered buds are induced to grow into rattan...

Embodiment 2

[0054] In this example, basic medium: select MS medium, sucrose or white sugar 20-30g / L, agar 7-9g / L, pH5.6-5.8; induction medium: MS+BA 0.1-5.0mg / L+NAA 0.0~0.5mg / L+sucrose or white sugar 30g / L; proliferation medium: MS+BA 0.1~3.0mg / L+NAA 0.0~0.5mg / L+sucrose or white sugar 30g / L; rooting medium: 1 / 2MS+NAA0 .1~1.0mg / L+sucrose or white sugar 20g / L.

[0055] All the other steps and conditions are the same as in Example 1.

Embodiment 3

[0057] In this example, basic medium: select MS medium, sucrose or white sugar 20-30g / L, agar 7-9g / L, pH5.6-5.8; induction medium: MS+KT 0.1-5.0mg / L+IAA 0.0~0.5mg / L+sucrose or white sugar 30g / L; proliferation medium: MS+KT 0.1~3.0mg / L+IAA 0.0~0.5mg / L+sucrose or white sugar 30g / L; rooting medium: 1 / 2MS+IAA 0.1~1.0mg / L+sucrose or white sugar 20g / L.

[0058] All the other steps and conditions are the same as in Example 1.

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Abstract

The invention discloses a tissue culture and rapid propagation method for campanumoea, which comprises the following steps of: 1) selecting and pretreating an explant; 2) sterilizing the explants; 3) performing inducing culture; 4) performing propagation culture; 5) performing rooting culture; and 6) transplanting, wherein a basic culture medium is an MS basic culture medium, comprises 20 to 30g / L of cane sugar or white sugar and 7 to 9g / L of agar, and has the pH of 5.6 to 5.8; an inducing culture medium comprises MS, 0.1 to 5.0mg / L of cytokinin, 0.0 to 0.5mg / L of growth hormone and 30g / L of cane sugar or white sugar; a propagation culture medium comprises MS, 0.1 to 3.0mg / L of cytokinin, 0.0 to 0.5mg / L of growth hormone and 30g / L of cane sugar or white sugar; and a rooting culture medium comprises half MS, 0.1 to 1.0mg / L of growth hormone and 20g / L of cane sugar or white sugar. The method has the advantages of simple and convenient operation, strong pertinence of component formulas of the culture media, low vitrification rate of tissue culture seedlings, feasible operation, and capacity of making the campanumoea have high propagation coefficient and good genetic stability than wild campanumoea.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a method for tissue culture and rapid propagation of Codonopsis pilosula. Background technique [0002] Codonopsis pilosula is the root of Campanumoea javanica B1. Subsp. Japonica (Makino) Hong or Campanumoea javanica Blume, a plant of the genus Campanum in Campanulaceae. Leopard (Earth Codonopsis) and Leopard large flower are perennial twining vines. Born on sunny slopes in low mountainous areas. Mainly distributed in East Asia, southern and southwestern my country. Taproot hypertrophy, fleshy, beige, few fibrous roots. It has the functions of invigorating the spleen and stomach, invigorating the lung qi, eliminating phlegm and relieving cough, etc. It can treat internal injuries due to fatigue, cough due to lung deficiency, diarrhea due to spleen deficiency, insufficient milk, malnutrition in children, enuresis and other diseases. The medi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 徐刚陈剑平汪一婷吕永平牟豪杰
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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