Tissue culture and rapid propagation method for campanumoea

A technology of tissue culture rapid propagation and soil Codonopsis pilosula, applied in horticultural methods, soilless cultivation, botanical equipment and methods, etc., can solve the problems of uneven quality of seedlings, difficulty in meeting high-quality seedlings, and lack of natural resources. To achieve the effect of strong pertinence, feasible operation, and expansion of sources
CN101869074AInactive Publication Date: 2010-10-27ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Publication Date
2010-10-27
Estimated Expiration
Not applicable · inactive patent
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Abstract

The invention discloses a tissue culture and rapid propagation method for campanumoea, which comprises the following steps of: 1) selecting and pretreating an explant; 2) sterilizing the explants; 3) performing inducing culture; 4) performing propagation culture; 5) performing rooting culture; and 6) transplanting, wherein a basic culture medium is an MS basic culture medium, comprises 20 to 30g / L of cane sugar or white sugar and 7 to 9g / L of agar, and has the pH of 5.6 to 5.8; an inducing culture medium comprises MS, 0.1 to 5.0mg / L of cytokinin, 0.0 to 0.5mg / L of growth hormone and 30g / L of cane sugar or white sugar; a propagation culture medium comprises MS, 0.1 to 3.0mg / L of cytokinin, 0.0 to 0.5mg / L of growth hormone and 30g / L of cane sugar or white sugar; and a rooting culture medium comprises half MS, 0.1 to 1.0mg / L of growth hormone and 20g / L of cane sugar or white sugar. The method has the advantages of simple and convenient operation, strong pertinence of component formulas of the culture media, low vitrification rate of tissue culture seedlings, feasible operation, and capacity of making the campanumoea have high propagation coefficient and good genetic stability than wild campanumoea.
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Description

technical field

[0001] The invention relates to the technical field of plant tissue culture and rapid propagation, in particular to a method for tissue culture and rapid propagation of Codonopsis pilosula. Background technique

[0002] Codonopsis pilosula is the root of Campanumoea javanica B1. Subsp. Japonica (Makino) Hong or Campanumoea javanica Blume, a plant of the genus Campanum in Campanulaceae. Leopard (Earth Codonopsis) and Leopard large flower are perennial twining vines. Born on sunny slopes in low mountainous areas. Mainly distributed in East Asia, southern and southwestern my country. Taproot hypertrophy, fleshy, beige, few fibrous roots. It has the functions of invigorating the spleen and stomach, invigorating the lung qi, eliminating phlegm and relieving cough, etc. It can treat internal injuries due to fatigue, cough due to lung deficiency, diarrhea due to spleen deficiency, insufficient milk, malnutrition in children, enuresis and other diseases. The medi...

Claims

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