120 results about "Genes proteins" patented technology

The invention provides a vaccine target screening system based on calculation model simulation. The vaccine target screening system comprises a multi-source heterogeneous database, wherein the multi-source heterogeneous database integrates and standardizes pathogenic genes, protein structures, literatures and experimental data; the feature calculation module calls a calculation biological model to carry out structural analysis, immunogenicity simulation and stability prediction; the intelligent screening and sorting module applies a multi-objective optimization algorithm to perform parallel evaluation and outputs optimal target spots; a structure iteration optimizer automatically iteratively corrects the optimized target spots to generate a high-potential variant library; and the process suitability simulation module couples the variants with the preparation formula and the process parameters to simulate production storage behaviors and feeds back an optimization target. According to the invention, efficient screening and optimization of vaccine targets can be realized, the accuracy and efficiency of target screening are improved, the research and development cost is reduced, and the research and development process of vaccines is accelerated.

The invention relates to a gene, a protein and a plasmid of a trachinotus ovatus apoptosis regulatory factor Bok and application of the gene, the protein and the plasmid, and belongs to the field of molecular biology, the sequence of cDNA nucleotide of the trachinotus ovatus apoptosis regulatory factor Bok is as shown in SEQ ID NO.1, and the amino acid sequence is as shown in SEQ ID NO.2. The invention further provides a construction method of the eukaryotic expression plasmid of the trachinotus ovatus apoptosis regulatory factor Bok. The regulation effect of the constructed eukaryotic expression vector on other apoptosis-related factors of the trachinotus ovatus is verified, and after the eukaryotic expression plasmid for the apoptosis regulation factor Bok of the trachinotus ovatus is injected into a fish body, the disease resistance of the fish body can be remarkably improved, and a remarkable immune protection effect is achieved.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention relates to the field of gene engineering, in particular to Os HSL mutants and coding genes, expression vectors, transgenic cells, compositions and application thereof, the mutants are Os HSL1 enzyme and / or Os HSL2 enzyme mutants, the amino acid sequence of the Os HSL1 enzyme is as shown in SEQ ID NO.1, and the amino acid sequence of the Os HSL2 enzyme is as shown in SEQ ID NO.2. The invention further relates to an application of the Os HSL mutants and the coding genes, the expression vectors, the transgenic cells and the compositions. The invention relates to a gene, a protein, a carrier, a cell and a composition which endow plants with a detoxification effect on an HPPD inhibitor and application of the gene, the protein, the carrier, the cell and the composition to plant improvement, so that the plants are endowed with resistance to the HPPD inhibitor.

The invention discloses an application of a composition of a polypeptide and a PARP inhibitor in preparation of a medicine for treating breast cancer, the sequence of the polypeptide is as shown in SEQ ID NO: 1, and the PARP inhibitor is Olaparil or taprazopalil. The polypeptide can down-regulate the expression of the DNA damage repair gene FANCD2 protein and block the cross-linking damage repair between DNA chains. The PARP inhibitor blocks DNA single-stranded fracture damage repair by inhibiting the catalytic activity of PARP, and is mainly used for breast cancer treatment of BRCA mutation. DNA damage caused by the polypeptide and the PARP inhibitor in two different modes jointly destroys a cell replication fork protection mechanism, so that a DNA replication fork of a breast cancer cell is prone to stagnation and collapse, and cell death is caused. The invention shows synergistic cancer suppression activity in both BRCA wild type and BRCA mutant breast cancer subtypes, and is expected to provide a new treatment strategy for clinical treatment of breast cancer.

As disclosed herein a precision based immunomolecular augmentation (PBIMA) high specificity patient profiling networked computer system, rapid therapeutic vaccine design method, and personalized vaccine, which utilizes immuno-molecular biopathway HLA affinity mapping and selection prediction ranking tools. This PBIMA approach comprises: Strategic-Selection, Molecular-Mapping, Antigen-Alignment, Receptor-Recognition, and Tactical Technology (SMART). The platform obtains data from a patient's genes and proteins as input. NGS data, including WES, WGS, ctDNA and cfDNA, RNAseq uses as input. PBIMA comprises a gene-protein-cell Cloud-based sequence editing interface to select the high confidence peptides. The PBIMA vaccine is a solution-based multi-purpose vaccine design strategy. PBIMA technology can produce therapeutic vaccines for cancer, autoimmune, neurodegenerative, inflammation-driven disease, and novel pathogen infection treatment. PBIMA therapeutic design is multi-mechanistic and broad-spectrum.

The present disclosure relates to a kit for detecting a soluble growth stimulation expressed gene 2 protein. In particular, the present disclosure relates to a latex-enhanced turbidimetric immunoassay kit for detecting the concentration and / or content of the sST2 in human samples. The kit can be used in transmission immunoturbidimetry and scattering immunoturbidimetry. The kit comprises a buffer system, an anti-interference component, latex microspheres, an anti-sST2 antibody, etc. The latex-enhanced immunoturbidimetric agent of the present disclosure can detect sST2 proteins within a range of <400 ng / ml in a sample, with a sensitivity of up to 0.1 ng / ml and a high specificity, accuracy and precision. The kit is suitable for a fully automatic biochemical analyzer and a scattering analyzer, and has the advantages of convenient and fast use and low cost, and can be used clinically to detect the sST2 protein.

The invention belongs to the technical field of gene engineering, and particularly relates to a larch drought response gene LgLEA10, a protein and application thereof. The nucleotide sequence of the larch drought response gene LgLEA10 provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the protein expressed by the larch drought response gene LgLEA10 is as shown in SEQ ID NO.2. It is proved for the first time that the LgLEA10 gene of dahurian larch is a drought response regulation gene of dahurian larch. Tests prove that overexpression of the LgLEA10 gene can improve the drought resistance of larch by remarkably improving the activity of antioxidant enzyme and osmotic adjusting substances. The discovery provides important gene resources and theoretical basis for cultivating a new variety of larch with strong drought resistance.

The invention provides a marker combination for predicting severe acute pancreatitis and application thereof. Specifically, the invention provides application of a gene, mRNA, cDNA and protein of a risk marker for judging severe acute pancreatitis or a detection reagent of the gene, the mRNA, the cDNA and the protein, and the gene, the mRNA, the cDNA and the protein are used for preparing / establishing a diagnostic reagent or a kit / equipment for judging the occurrence risk of severe acute pancreatitis. Researches show that the severe acute pancreatitis risk marker can be used as a marker for early judgment of whether acute pancreatitis patients develop into severe acute pancreatitis, has high sensitivity and specificity, can quickly diagnose severe acute pancreatitis at a relatively early disease progress stage, and provides powerful assistance for early treatment intervention of diseases.

This invention discloses the application of the OsCAD3 protein and its encoding gene in regulating resistance to bacterial blight in rice, relating to the field of plant genetic engineering. The amino acid sequence of this gene protein is shown in SEQ ID NO.3. Cloning the gene editing site sequence, constructing a gene editing recombinant vector, and transforming it into rice can improve rice resistance to bacterial blight and ensure stable rice yield. The OsCAD3 gene editing vector constructed in this invention and the new OsCAD3 allele formed through genome editing can be applied to rice breeding for resistance to bacterial blight. Constructing an overexpression recombinant vector of the above gene coding region sequence and transforming it into rice can reduce rice resistance to bacterial blight and ensure stable rice yield. The above gene overexpression vector can be used in rice breeding to regulate bacterial blight. This invention can be used in the fields of rice germplasm resource improvement and genetic breeding, and is of great significance for breeding new rice varieties resistant to bacterial blight.

The present invention relates to an ACCS gene, a protein and the use thereof. An involved ACCS protein mutant is formed by means of mutating a proline residue at position 134 and a tryptophan residue at position 136 of an animal-derived wild-type ACCS protein into a glutamine residue and a tyrosine residue, respectively. The involved ACCS protein mutant can be used in the intervention and treatment of a variety of diseases comprising brain diseases, and provides a new regimen and approach for the intervention and treatment of brain diseases.

The invention discloses application of NSD2 in prevention and treatment of liver cancer. Specifically, the invention provides application of an NSD2 gene, mRNA, protein or an expression promoting agent thereof, the NSD2 gene, the mRNA, the protein or the expression promoting agent is used for preparing a preparation or a composition, and the preparation or the composition is used for preventing and / or treating liver cancer. The NSD2 gene, the mRNA, the protein or the expression promoting agent thereof can be used for effectively treating liver cancer.

The application discloses a tobacco NtGLV11 gene, a protein, and polypeptide coding, and application and preparation and a preparation method of the polypeptide in promoting plant yellowing. The tobacco NtGLV11 gene codes the NtGLV11 protein, and the NtGLV11 polypeptide is generated after the NtGLV11 protein is cut and chemically modified. It is found for the first time that the NtGLV11 polypeptide is involved in tobacco yellowing regulation, and no report is found in crops including tobacco at present. The NtGLV11 polypeptide hormone of the application promotes tobacco senescence and yellowing, and has simple preparation and use methods, strong operability, effectively promotes tobacco senescence and yellowing, and has biological safety.

The invention discloses a tobacco NtMATE62 gene, a protein and application of the tobacco NtMATE62 gene in regulation and control of flavonol synthesis and drought resistance. The invention discloses a method for obtaining a plant with drought resistance and / or rich in flavonol, and the method comprises the step of increasing the expression quantity of an NtMATE62 gene in the plant so as to obtain the plant with drought resistance and / or rich in flavonol. The tobacco NtMATE62 gene plays an important role in tobacco flavonol metabolism and drought resistance regulation and control processes, and can be applied to promotion of flavonol biosynthesis and drought-resistant plant breeding.

The application discloses a tobacco NtGLV05 gene, a protein, and polypeptide coding, and application and preparation and a preparation method of the polypeptide in promoting plant yellowing. The tobacco NtGLV05 gene codes the NtGLV05 protein, and the NtGLV05 polypeptide is generated after the NtGLV05 protein is cut and chemically modified. It is found for the first time that the NtGLV05 polypeptide is involved in tobacco yellowing regulation, and no report is found in crops including tobacco at present. The NtGLV05 polypeptide hormone of the application promotes tobacco senescence and yellowing, and has simple preparation and use methods, strong operability, effectively promotes tobacco senescence and yellowing, and has biological safety.

The present application provides a kind of PRMT5 inhibitor, PRMT5 inhibitor includes: recombinant adeno-associated virus vector AAV-shPrmt5 and GSK3326595;Recombinant adeno-associated virus vector AAV-shPrmt5 includes the shRNA of targeting PRMT5, the nucleotide sequence of shRNA is as shown in SEQ ID No:1.The present application also provides the application of the above-mentioned PRMT5 inhibitor in the preparation of drug for improving primary biliary cholangitis, PRMT5 inhibitor is all PRMT5 gene / protein as target point, by inhibiting the expression of PRMT5 gene / protein, reduce liver tissue and spleen tissue Tfh cell and GC B cell, serum autoantibody and transaminase level, can effectively delay the disease progression of primary biliary cholangitis.

The invention provides a method for constructing an emphysema mouse airway candida colonization model, and belongs to the technical field of candida colonization models.The method comprises the following steps that 1, an emphysema mouse model is constructed, including tobacco smoke exposure and elastase titration; and 2, establishing the pulmonary emphysema mouse Candida albicans airway colonization model, including preparation of a Candida albicans suspension and airway Candida colonization. The genomes of mice are closer to those of human beings than those of other animals, the mice have relatively wide gene, protein sequence and antibody availability, and the modeling cost of the mice is low, so that the mice are more suitable for experimental research of emphysema. Therefore, the construction method of the model is closer to a natural generation mode, is simple and convenient, can better simulate disease characteristics of candida albicans airway colonization in COPD, and creates conditions for further researching whether candida albicans airway colonization has a certain relationship with occurrence and development of COPD and acute exacerbation thereof.

The invention belongs to the technical field of biotechnology and medicine, and particularly relates to application of an Anapc11 gene / protein to screening of drugs for treating hyperuricemia-related multi-organ dysfunction. According to the invention, the Anapc11 gene is used as a molecular target or a drug action target, and is used for screening or preparing drugs for treating multi-organ dysfunction (including liver injury, testis dysfunction, intestinal barrier destruction and the like) caused by hyperuricemia. The invention provides a new application of the cell cycle promoting complex subunit E3 ubiquitin ligase Anapc11 gene, provides a new molecular target for treating hyperuricemia related multi-organ dysfunction, and breaks through the limitation that the existing uric acid reducing medicine is only limited to a metabolic link; a new direction and a good application prospect are provided for developing innovative medicines with organ protection effects.

The present disclosure provides engineered extracellular vesicles (e.g., exosomes) comprising a fusion polypeptide that includes a Tumor Necrosis Factor-Stimulated Gene-6 (TSG-6) protein or a truncated form thereof displayed on the vesicle surface in a stabilized and biologically active configuration. The engineered extracellular vesicles retain or enhance the anti-inflammatory and cytoprotective activities of TSG-6 and may be used in the prophylaxis or treatment of inflammatory, autoimmune, or injury-related diseases or disorders. Also provided are nucleic acid constructs encoding the fusion polypeptide, compositions comprising the engineered extracellular vesicles, methods for producing the vesicles, and methods for using the vesicles in therapeutic applications.

The invention discloses an application of an Mbtps1 gene and a protein SKI-1 coded by the Mbtps1 gene in screening or preparing a medicine for treating cerebral arterial thrombosis, and particularly discloses an application of the Mbtps1 gene / protein SKI-1 as a medicine target of cerebral arterial thrombosis. A tMCAO mouse model is constructed by specifically knocking out a microglial cell Mbtps1 gene / protein SKI-1 of a tMCAO mouse to verify that the Mbtps1 gene and the protein SKI-1 can be used as drug targets for screening or preparing drugs for treating cerebral arterial thrombosis; the compound PF429242 is screened out and has the functions of repairing brain injury nerves caused by cerebral arterial thrombosis, inhibiting neuroinflammation and repairing fragmentation of a mitochondrial network, namely, the feasibility of the Mbtps1 gene and the protein SKI-1 serving as targets in screening or preparing medicines for preventing or treating cerebral arterial thrombosis is verified.

The application provides a gene and a protein sequence of a deep archaea transmethylase I and a preparation method thereof, belongs to the technical field of genetic engineering, and the transmethylase I is derived from a deep archaea genome and has transmethyl activity, so that the methyl in a methoxyl aromatic compound is transferred to a corrin ring protein. The deep archaea transmethylase I is cloned into a pCold-TF expression vector, is induced to express in Escherichia coli through a recombination expression vector, is purified through immobilized nickel ion affinity, and the transmethylase is prepared. The transmethylase I can be used for transmethylation of the methoxyl aromatic compound.

This invention relates to the strawberry FaCMT3.1 gene and its application in regulating fruit color quality and postharvest preservation quality, belonging to the field of agricultural bioengineering technology. The FaCMT3.1 gene, with a full-length protein-coding sequence (CDS) of 2892 bp, was cloned from strawberry and its function was identified. The results showed that FaCMT3.1 negatively regulates anthocyanin accumulation in strawberry fruit. Increased FaCMT3.1 expression inhibits strawberry coloring, while decreased expression promotes it. Therefore, by rationally utilizing this gene, strawberry fruit color can be regulated, postharvest preservation quality can be improved, and color-related strawberry breeding can be guided. This invention provides a theoretical basis and technical support for using genetic engineering technology to regulate anthocyanin content in fruit, and has significant application value.

The application discloses application of a tobacco NtMATE34 gene and protein in regulation of a bacterial wilt resistance. The tobacco NtMATE34 gene is induced to express by inoculation of a bacterial wilt pathogen Ralstonia solanacearum, and participates in a tobacco bacterial wilt stress response. After artificial inoculation of the bacterial wilt, compared with K326, the tobacco plant with overexpression of the NtMATE34 gene shows strong bacterial wilt resistance. The tobacco NtMATE34 gene plays an important role in regulation of the tobacco bacterial wilt resistance, and can be applied to gene function research and biological breeding for improvement of the tobacco bacterial wilt resistance.

The invention discloses an application of FAM20C protein or a coding gene thereof in preparation of an anti-RSV drug, and relates to the technical field of gene functions and application, and the FAM20C protein or the coding gene thereof is an action target of the drug; 1, and the amino acid sequence of the FAM20C protein is as shown in SEQ ID NO. 2. According to the invention, the relationship between the FAM20C gene / protein and RSV infection is determined, the FAM20C gene / protein can be used as a therapeutic target of RSV infection, the replication of RSV virus can be inhibited by knocking down the FAM20C gene, and the inhibitor FL-1607 of the FAM20C gene can be used for treating RSV infection. The invention provides an effective new way for the treatment of RSV (Respiratory Syndrome Virus) infection.

The invention relates to the field of biological detection, in particular to a multi-omics combined screening cell senescence marker detection method and application thereof. According to the method, data of genomics, transcriptomics, proteomics and metabonomics are combined, and multi-dimensional data in the cell senescence process are subjected to high-throughput screening and integration by means of whole genome sequencing, RNA-Seq, a liquid chromatography-mass spectrometry technology and a gas chromatography-mass spectrometry technology. And adopting machine learning algorithms such as random forest, a support vector machine and deep learning to screen out key markers related to aging. The markers comprise genes, proteins and metabolites, and have relatively high sensitivity and specificity. Verification of different aging models proves that the screened markers change significantly in the aging process and can effectively reflect the biological characteristics of cell aging. The method provides new technical support and theoretical basis for research of an aging mechanism, early diagnosis of aging-related diseases and anti-aging treatment.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of preventing and treating verticillium wilt by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHAT1 (the encoding protein of the verticillium dahliae pathogenic gene VdHAT1 is as shown in SEQ ID NO: 3), the core of the verticillium dahliae pathogenic gene VdHAT1 is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 4, SEQ ID NO: 6 or SEQ ID NO: 7. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

A R gene is arranged for controlling soybean-rhizobia compatibility, wherein the GmNNL1 gene of the gene sequence in Hengfeng black bean of the soybean products is shown in SEQ ID NO. 2, and the encoded amino acid sequence is shown in SEQ ID NO. 3. The soybean R gene GmNNL1 is an effective gene that is able to regulate the nodule number of specific rhizobia on soybean, and to regulate the nodule number by directly recognizing the haplotype of the bradyrhizobia-specific effector protein NopP, which is able to limit the symbiotic nodulation of indigenous rhizobia, make soybean preferentially nodule with artificially applied high-efficiency rhizobia agents, and improve symbiotic nitrogen fixation capacity.

The invention discloses a tobacco NtMATE30 gene, a protein and application of the tobacco NtMATE30 gene in regulation and control of flavonol synthesis and drought resistance. The invention discloses a method for obtaining a plant with drought resistance and / or rich in flavonol, and the method comprises the step of increasing the expression quantity of an NtMATE30 gene in the plant so as to obtain the plant with drought resistance and / or rich in flavonol. The tobacco NtMATE30 gene plays an important role in tobacco flavonol metabolism and drought resistance regulation and control processes, and can be applied to promotion of flavonol biosynthesis and drought-resistant plant breeding.

The invention relates to the technical field of genetic engineering, in particular to a gene MEU26 related to sexual coordination and pathopoiesis of sugarcane sporosmut, protein and application of the gene MEU26. The gene MEU26 is as shown in SEQ ID NO. 1. A delta MEU26 mutant strain is constructed through a PEG-mediated protoplast transformation method, a backfill strain is constructed through an ATMT method, it is found that the sexual cooperation ability and pathogenicity of the delta MEU26 mutant strain are affected, the sexual cooperation ability and pathogenicity of the backfill strain are basically recovered, and it is indicated that the MEU26 gene participates in regulation and control of the sexual cooperation and pathogenicity of the Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis Saccharospora canadensis. A foundation is provided for screening and developing products for reducing pathogenicity of sugarcane sporoderm smut, and a new target is provided for researching and developing agents for preventing and treating sugarcane smut and a novel efficient disease prevention and control technology.