375 results about "Genes proteins" patented technology

The invention provides a SARS-CoV-2 vaccine using human type-5 replication-defective adenovirus as a vector. The vaccine uses E1 and E3 to be combined with replication-defective human type-5 adenovirus as the vector and HEK293 cells integrating adenovirus E1 gene as a packaging cell line, and a protective antigen gene carried is the 2019 SARS-CoV-2 S protein gene (Ad5-nCoV) which is subjected to optimization design. After the S protein gene is optimized, the expression level in transfected cells is increased significantly. The vaccine has good immunogenicity in mouse and guinea pig models, andcan induce a body to produce a strong cellular and humoral immune response in a short time. Studies on the protective effect of hACE2 transgenic mice show that after 14 days of single immunization ofAd5-nCoV, the viral load in lung tissue can be significantly reduced, and it is indicated that the vaccine has a good immunoprotective effect on the 2019 SARS-CoV-2. In addition, the vaccine is quick, simple and convenient to prepare, and can be mass-produced in a short period of time to respond to sudden outbreaks.

The present invention provides compositions, kits, assembles of articles and methodology for detecting multiple target molecules in a sample, such as in a tissue sample. In particular, site-specific deposition of elemental metal is used in conjunction with other means of detection, such as other chromogenic, radioactive, chemiluminescent and fluorescent labeling, to simultaneously detect multiple targets, such a gene, a protein, and a chromosome, in a biological sample. More particularly the multiple targets may be labeled with the specifically deposited metal and other chromogenic labels to allow chromogenic immunohistochemical (IHC) detection in situ by using bright field light microscope.

The invention described herein provides for methods of profiling cellular models of disease. Cellular systems biology is the investigation of the integrated and interacting networks of genes, proteins, and metabolites that are responsible for normal and abnormal cell functions. Methods and reagents for the profiling a disease state, the treatment of a disease state and assaying of treatments of a disease state are provided.

Disclosed is an one-dimensional biochip belonging to a serial analytic technique for micro-total analysis systems. The one-dimensional biochip is provided with a plurality of cellules on the micro-separation channel of micro flow control chip, microparticles modified by different biomolecules on surface are arranged in different cellules; in the gene or protein analysis, when the sample flows through the microchannel with a plurality of cellules, the microparticles can specifically identify and capture multiple target molecules, then a reagent with fluorescence labels can be introduced, and finally the microparticles will specifically combine with the fluorescence labels on surface to be detected by fluorescent imaging. The one-dimensional biochip provided by the invention not only has advantages of micro flow control technology and array analysis, but also improves the detection sensitivity and identification capability of target molecules, and provides an effective research measure for the gene and protein expression analysis at single cell level, and tumor research and drug screening.

Proteins having activity that promotes STAT6 activation, which are used for diagnosing, treating or preventing diseases associated with the excessive activation or inhibition of STAT6 are provided. Using a STAT6 response reporter plasmid, cDNA encoding a protein capable of promoting STAT6 activation was cloned from the cDNA library constructed from human lung fibroblasts, and the DNA sequence and the deduced amino acid sequence are determined. The protein, the DNA encoding the protein, a recombinant vector containing the DNA, and a transformant containing the recombinant vector are useful for screening a substance inhibiting or promoting STAT6 activation.

The present invention describes the development of a positive selection vector based on regulatory element modulation, wherein such modulation is achieved via insertional reconstruction or destruction of a regulatory element controlling transcription, translation, DNA replication and termination. A positive selection cloning vector pREM5Tc has been developed based on insertional reconstruction of a regulatory element of a reporter gene. The vector pREM5Tc carries the tetracycline resistance reporter gene with no functional -35 region of its promoter, a regulatory element, thus resulting in no expression of the tetracycline resistance gene. Hence a host cell carrying the vector pREM5Tc is unable to produce the tetracycline resistance gene protein resulting in inhibition of its growth in presence of tetracycline. An E. coli consensus -35 region is recognized as 5'-TTGACA-3' and a primer used in polymerase chain reaction (PCR) carries at its 5' end the sequence 5'-TGTCAA-3', which is the complementary sequence of 5'-TTGACA-3'. The PCR-amplified DNA fragment is ligated to pREM5Tc thus reconstructing the functional promoter of the tetracycline resistance reporter gene. Subsequent transformation of a host cell with the recombinant vector (carrying an insert DNA) results in production of the tetracycline resistance reporter gene protein that confers resistance to tetracycline thus allowing only the recombinants to grow in presence of tetracycline. The positive selection vector pREM5Tc greatly reduces, if not eliminates, the number of exonuclease-generated false positive clones.

The invention discloses a pig mycoplasma pneumonia recombination antigen ELISA detection Kit. The Kit is provided with an antibody detection plate, enzyme conjugate treatment fluid, a positive control, a negative control, sample diluent, 10x condensed cleaning solution, developing solution A, developing solution B and termination solution. The detection plate of the Kit is a detachable 96-pore enzyme label plate enveloped by the mutational pig mycoplasma pneumonia membrane protein P46 gene protein antigen, the enzyme conjugate treatment fluid is a rabbit anti-pig antibody labeled by horse radish peroxidase, the positive control serum is taken from a pig which is detected positive through indirect hemagglutination and the ELISA Kit of IDEXX and has obvious pig mycoplasma pneumonia lesions in the lungs after anatomy, and the negative control serum is taken from a pig which is detected negative through indirect hemagglutination and the ELISA Kit of IDEXX and has no pig mycoplasma pneumonia lesions in the lungs after anatomy. The pig mycoplasma pneumonia recombination antigen ELISA detection Kit has the advantages of strong specificity, high sensitivity, simple operation, easy large-scale generation and application, and broad market prospect.

Systems and methods for modeling the interactions of the several genes, proteins and other components of a cell, employing mathematical techniques to represent the interrelationships between the cell components and the manipulation of the dynamics of the cell to determine which components of a cell may be targets for interaction with therapeutic agents. A first such method is based on a cell simulation approach in which a cellular biochemical network intrinsic to a phenotype of the cell is simulated by specifying its components and their interrelationships. The various interrelationships are represented with one or more mathematical equations which are solved to simulate a first state of the cell. The simulated network is then perturbed by deleting one or more components, changing the concentration of one or more components, or modifying one or more mathematical equations representing the interrelationships between one or more of the components. The equations representing the perturbed network are solved to simulate a second state of the cell which is compared to the first state to identify the effect of the perturbation on the state of the network, thereby identifying one or more components as targets. A second method for identifying components of a cell as targets for interaction with therapeutic agents is based upon an analytical approach, in which a stable phenotype of a cell is specified and correlated to the state of the cell and the role of that cellular state to its operation. A cellular biochemical network believed to be intrinsic to that phenotype is then specified by identifying its components and their interrelationships and representing those interrelationships in one or more mathematical equations. The network is then perturbed and the equations representing the perturbed network are solved to determine whether the perturbation is likely to cause the transition of the cell from one phenotype to another, thereby identifying one or more components as targets.

The invention relates to a recombinant fusion protein vaccine and an attenuated live vector vaccine for treating and preventing helicobacter pylori (Hp) infection, and belongs to the field of biopharmaceutics. The recombinant fusion protein vaccine and the attenuated live vector vaccine for expressing the recombinant fusion protein are characterized in that: the recombinant fusion protein is formed by connecting immune protective function fragments of helicobacter pylori cytotoxin relevant gene protein CagA from helicobacter pylori, vacuolization cytotoxin VacA and urease subunit UreB linearly, and the immunogenicity and immune protection of the recombinant fusion protein are verified through animal experiments. The live vaccine has the advantages that: 1, an Hp fusion protein gene can beexpressed stably; 2, mucosa and systemic immune response can be induced after immunity; and 3, the method is convenient, the cost is low, and the economic benefit is obvious. Therefore, the attenuated live vector vaccine can be used as candidate vaccines for treating and preventing the Hp infection.

A yolk antibody for SARS coronavirus is prepared through injecting the antigen, which may be recombinant genetic protein S, M, or E,or the antigen epitope for said protein able to represent SARS coronavirus, or the synthetic polypeptide of said protein, etc, in health hen for primary immunizing, booster immunizing, collecting its egg, extracting yolk, and extracting the yolk antibody from it. It can also be prepared to become liquid preparation. It can be used to prevent SARS.

The present invention concerns genes containing mutations associated with autism its onset and development and also to the encoded proteins of said genes associated with autism, its onset and development and the use of said genes, proteins or protein isoforms. The invention thus also relates to methods of screening for, diagnosis and treatment of autism in human subjects e.g., clinical screening, diagnosis, prognosis, therapy and prophylaxis, as will as for drug screening and drug development.

The invention discloses a Parkinson's disease gene diagnosis kit which comprises 11 pathogenic genes of the Parkinson's disease namely SNCA,Parkin,Pink1,UCHL-1,DJ-1,ATP13A2,GIGYF2,HTRA2,FBX07,Vps35 and MAPT. A detection method comprises the following steps: designing and synthesizing specific primers of all genes, collecting specimens of individuals to be detected, sequencing specific DNA fragments obtained by all genes through a RT-PCR technology, comparing the gene sequence with a normal gene sequence, and analyzing whether deleterious mutations exit or not to evaluate disease risks of the individuals. By adopting the Parkinson's disease gene diagnosis kit disclosed by the invention, different deleterious mutations of a pathogenic gene protein coding region (CDS) can be detected at a time, the method is simple, convenient and rapid, the specificity is good, the sensitivity is high and the Parkinson's disease gene diagnosis kit can be used for pathogenic gene screening and early diagnosis of the Parkinson's disease.

The invention relates to a recombinant virus containing a gene expression box expressing an anthropogenic GAD gene and a cell factor, in particular to a method for constructing a recombinant adeno-associated virus with the coexpression of an anthropogenic GAD 65 gene and a GDNF gene, an application and a meaning thereof. Anthropogenic GAD 65 gene and the GDNF gene protein products generated by the expression of the recombinant virus have favorable synergistic effect on the treatment of diseases relative to Parkinson's disease and the central nervous system and have important values for treatment of relative diseases.

The present invention provides cloning sequencing, analyzing, function research of a biosynthesis gene cluster of an antibiotic-Azinomycin B having antitumor activity produced by streptomyces, and its application. The whole gene cluster includes 34 genes: one repeatedly using I type polyketide synthase gene; two naphthalene ring modification enzyme genes; 8 non-ribosomal polypeptide skeleton synthesis and modification enzyme genes; 11 non-natural amino acid structure unit synthase genes; 1 resistance gene; 3 post modification enzyme genes and 8 genes which functions are not determined. The genetic operation of the biosynthesis gene breaks the synthesis of Azinomycin B; the precursor compound is produced by the heterologous expression of synthesis gene and modification gene of naphthalene ring. The gene of the invention and the protein can be used for searching and finding compound or gene, protein applied in medical, industry or agriculture.

Canavan disease, an autosomal recessive leukodystrophy, is caused by deficiency of aspartoacylase and accumulation of N-acetylaspartic acid in brain. Human aspartoacylase (ASP) cDNA spanning 1,435 bp has been cloned and expressed in E. coli. A base change, a854>c, has been found in 85% of the 34 Canavan alleles tested so far, which results in a missense glu285>ala mutation that is predicted to be part of the catalytic domain of aspartoacylase. The invention therefore provides nucleic acid sequences, genes, polypeptides, antibodies, vectors containing the gene, host cells transformed with vectors containing the gene, animal models for the disease, methods for expressing the polypeptide, genetic screening methods and kits, diagnostic methods and kits, methods of treating Canavan disease and methods of genetic therapy for the disease.

The invention discloses application of an OsAGO18 gene in improving the rice stripe disease resistance of rice, and provides application of an OsAGO18 gene, encoding protein of the OsAGO18 gene, or a recombinant carrier containing the encoding gene, in regulating and controlling rice stripe disease resistance of plants. The amino acid sequence of the gene OsAGO18 protein is specifically shown in the sequence 2 in a sequence table. The experiment shows that the plant disease resistance in rice with over-expression of the OsAGO18 gene is improved; on the contrary, the disease resistance of an ago18 mutant is degraded, which means that the OsAGO18 protein encoded by the gene plays an important role in rice stripe disease resistance of rice.

The invention belongs to the technical field of plant gene engineering, and relates to clone and application of a maize water-logging tolerance-related transcription factor gene zm-bRLZ. The nucleotide sequence of the gene is shown as SEQ ID No. 1, and the gene has a total length of 4,027bp and comprises 6 exons. The cDNA sequence of the gene is shown as SEQ ID No. 2, and 282 amino acids are encoded on the gene. In flooding stress, the expression of the gene in maize inbred line Hz32 seedling roots is up-regulated and the expression level of the gene in the Mo17 is kept unchanged. The in-vitro combination of a gene protein product and an antidiuretic hormone (ADH) promoter anaerobic response factor shows that the zm-bRLZ gene has a regulation and control effect on an anaerobic induced gene. A pair of cleaved amplified polymorphic sequence (CAPS) markers is developed by utilizing zm-bRLZ gene sequence difference of the maize water-logging tolerance inbred line Hz32 and high-sensitivity K12. The gene is positioned at a water-logging tolerance quantitative trait locus (QTL) peak part of 9.04bin by utilizing K12*Hz32 F2 group. A candidate gene correlation analysis proves that a zm-bRLZ gene promoter region and a plurality of single nucleotide polymorphism (SNP) sites at a 3'-untranslated region (3'-UTR) are all obviously associated with a plurality of water-logging tolerance indexes.

The invention discloses a biosynthetic gene cluster of romatic-polyketide atypical fluostatins and applications thereof. The nucleotide sequence of the biosynthetic gene cluster of fluostatins derived from micromonospora rosaria SCSIO N160 (preservation No: CCTCC NO: M2012392) is shown in the 1st-40128th base sequences in SEQ ID NO. 1, and contains 32 genes. Genes and proteins thereof provided by the invention can be used for seeking and finding compounds or genes and proteins for medicine, industry or agriculture.

The present invention relates to the development of a positive selection vector based on insertional reconstruction of a reporter gene or of a regulatory gene controlling the expression of a reporter gene. The cloning vector carries a reporter gene or a regulatory gene with a mutation rendering the reporter or the regulatory gene protein functionally inactive. A primer carrying a nucleic acid sequence that corrects the mutation is used during PCR amplification of a targeted nucleic acid sequence, and the amplified DNA fragment is then ligated to the said vector thus reconstructing the wild-type reporter or regulatory gene.

The invention provides a method for information mining of a genome metabolic network preliminary model. The method comprises the steps as follows: as for the KEGG website, extracting a corresponding relation among gene, protein and reaction, that is, determining the GPR relation by adopting a website script semantic analysis technology on the basis of webpage key content priori position information, and establishing an excel table to show the GPR relation information, so as to obtain the genome metabolic network preliminary model. The model constructed by adopting the method unifies the reaction form, and is convenient in comparison with other models and convenient in reference. The method has been applied to construction of a scheffersomyces stipitis CBS6054 genome-scale metabolic network, and compared with the construction of a traditional network preliminary model based on the KEGG database, a great amount of labor and time are saved, and the construction efficiency is greatly improved.

The invention discloses a genetic genetic diagnosis kit for amyotrophic lateral sclerosis. The kit and a detection method are characterized in that gene-specific primers are designed and synthesized according to a virulence gene which is studied and reported to be correlated with the amyotrophic lateral sclerosis, including SOD1, FUS, VAPB, ANG, TARDBP, FIG4, OPTN, VCP, Sigmar1, CHMP2B and PFN1 sites, a sample is obtained from an individual to be detected, a RT-PCR technology is carried out, the RT-PCR product is subjected to sequencing analysis, next, the sample sequence is compared with a normal gene sequence to determine whether the sample sequence has disease-causing mutations. The kit is capable of detecting all disease-causing mutation sites of the coding sequence (CDS) of the virulence gene at a time, and has the advantages of being simple and quick, reliable in preparation, good in specificity, high in sensitivity and the like; the kit is capable of detecting the ALS patients and asymptomatic patients.

The invention discloses a rice grain type growth and development related coding gene, and the name of the rice grain type growth and development related coding gene is OrMKK3-4; and the rice grain type growth and development related gene OrMKK3-4 is used for regulating rice grain growth and development. The application firstly identifies the rice grain type growth and development related gene OrMKK3-4, and the rice grain type growth and development related gene OrMKK3-4 can obtain the short grain rice under the condition of enhanced function or increased expression, which proves that the ricegrain type growth and development related gene protein or its protein plays an important role in controlling rice grain size.

The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal.

The invention provides an anti-ageing gene oligopeptide-containing preparation and an application of the preparation to cosmetics. The preparation is prepared by compounding gene oligopeptide key components, auxiliary materials and preparation raw materials. Gene oligopeptide is selected from one or a combination of genetic recombination hydrolyzed collagen dipeptide, genetic recombination hydrolyzed collagen tripeptide, genetic recombination hydrolyzed collagen tetrapeptide, genetic recombination hydrolyzed collagen pentapeptide, genetic recombination hydrolyzed collagen hexapeptide, first genetic recombination hydrolyzed collagen, second genetic recombination hydrolyzed collagen, third genetic recombination hydrolyzed collagen, genetic recombination gene protein, first genetic recombination hydrolyzed hyaluronic acid and second genetic recombination hydrolyzed hyaluronic acid.

The invention discloses a recombinant carp Nrf2 (NF-E2-related factor 2) gene, a recombinant carp Nrf2 protein, preparation and detection methods and application of the recombinant carp Nrf2 gene. The recombinant carp Nrf2 gene has a sequence shown by SEQIDNO:1, and the recombinant carp Nrf2 protein has a sequence shown by the SEQIDNO:2. By the nucleotide sequence of the carp Nrf2 gene complementary deoxyribonucleic acid (cDNA) disclosed by the invention, the tissue specific expression of the Nrf2 gene can be further detected through a reverse transcription-polymerase chain reaction (RT-PCR) or hybridization method, and the full-length cDNA of the carp Nrf2 gene can be further cloned. The complete Nrf2 protein may be expressed through recombining the cDNA of the recombinant carp Nrf2 gene onto an expression vector, so that the Nrf2 protein can be used for producing antibodies, and the Nrf2 gene expression states of cells, tissues, early embryos and individuals of carps under different states are detected.

The invention discloses a malus hupehensis MhWRKY40a gene and applications thereof. The nucleotide sequence of the malus hupehensis MhWRKY40a gene is shown as the SEQ ID NO.1. The MhWRKY40a gene is capable of applying to the production of novel species, which are salt resistant and low temperature resistant, and / or plant variety improvement. A novel MhWRKY40a gene is cloned from malus hupehensis for the first time, and the homology between the gene sequence and the arabidopis thaliana, grape and barley is 50.89%. But the protein tertiary structure of the MhWRKY40a is prominently different from the homologous gene of the model plant arabidopis thaliana; the former has 5 beta-folds, while the latter has 4 beta-folds. The MhWRKY40a transgenetic tobacco has the properties of salt resistance and low-temperature resistance, raises the resistance-associated gene expression, and therefore the fact, which the MhWRKY40a gene has the function of improving the salt resistance and low-temperature resistance properties of the plants, is testified.

The invention discloses a method for constructing a corynebacterium constitutive expression vector promoter based on transcriptome sequence. The invention further discloses the corynebacterium constitutive expression vector promoter constructed based on the transcriptome sequence, an expression vector containing the promoter and a recombination strain obtained by converting host cell corynebacterium glutamicum by the expression vector. According to the expression vector constructed by the promoter obtained by the method, the target gene protein expression quantity of the logarithmic phase is low, but the target gene protein expression quantity of the stable phase is greatly improved relative to the logarithmic phase, and the effect to the host bacteria growth is low, and the stability of the plasmid passage is high. The expression vector is suitable for constructing the vector of the target gene of which the logarithmic phase does not need the expression, but the stable phase needs the high expression, and is particularly suitable for constructing engineered strains for amino acid fermentation.

The invention relates to preparation and application of an egg yolk antibody containing florfenicol drug resistance gene protein (FloR). Riemerella anatipestifer (CCTCC No: M208128) resistant to florfenicol is separated from a duck dies clinically; PCR is adopted to amplify a section of the drug resistance gene (floR); the section of the drug resistance gene is cloned to an expression plasmid pET28a (+) for transfecting host bacteria BL21 (DE3). The recombination strain is subject to induction expression, and fusion protein containing the target gene section and having a HIS label is prepared; the fusion protein exists in the form of an inclusion body; the inclusion body is extracted for the preparation of an immunogen; the immunogen is used for immunizing a laying hen, so as to obtain the egg yolk antibody capable of improving the sensitivity of the drug resistance strain to florfenicol; the egg yolk antibody is used in combination with florfenicol to remarkably improve the treatment effect on a duck infected with a drug-resistant riemerella anatipestifer strain ; as other strains resistant to florfenicol are also mediated through a floR gene, the egg yolk antibody can be applied to the veterinary clinical treatment on livestock and poultry infected with strains resistant to florfenicol.