The invention discloses a genetic genetic diagnosis kit for amyotrophic lateral sclerosis. The kit and a detection method are characterized in that gene-specific primers are designed and synthesized according to a virulence gene which is studied and reported to be correlated with the amyotrophic lateral sclerosis, including SOD1, FUS, VAPB, ANG, TARDBP, FIG4, OPTN, VCP, Sigmar1, CHMP2B and PFN1 sites, a sample is obtained from an individual to be detected, a RT-PCR technology is carried out, the RT-PCR product is subjected to sequencing analysis, next, the sample sequence is compared with a normal gene sequence to determine whether the sample sequence has disease-causing mutations. The kit is capable of detecting all disease-causing mutation sites of the coding sequence (CDS) of the virulence gene at a time, and has the advantages of being simple and quick, reliable in preparation, good in specificity, high in sensitivity and the like; the kit is capable of detecting the ALS patients and asymptomatic patients.