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A kind of Streptococcus suis indirect hemagglutination detection kit and its application

A detection kit, Streptococcus suis technology, applied in the direction of biological testing, measuring devices, material inspection products, etc., can solve the problems of misdiagnosis and missed diagnosis of Streptococcus suis infection, loss of animal husbandry production, complex symptoms and lesions, etc., to achieve easy Long-term storage, low price, avoid the effect of short storage time

Active Publication Date: 2018-05-15
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the symptoms and lesions of this disease are more complicated, and it is easy to be confused with acute swine erysipelas, acute swine fever and other diseases, and it is easy to cause misdiagnosis and missed diagnosis of Streptococcus suis infection at the grassroots level, thus causing unnecessary losses to animal husbandry production

Method used

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  • A kind of Streptococcus suis indirect hemagglutination detection kit and its application
  • A kind of Streptococcus suis indirect hemagglutination detection kit and its application
  • A kind of Streptococcus suis indirect hemagglutination detection kit and its application

Examples

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Effect test

Embodiment 1

[0026] Example 1 Preparation of Streptococcus suis indirect hemagglutination diagnostic kit

[0027] The components of the Streptococcus suis indirect hemagglutination diagnostic kit are as follows:

[0028] (1) 1 bottle of Streptococcus suis indirect hemagglutination antigen, 10ml / bottle;

[0029] (2) 1 bottle of standard positive serum, 1ml / bottle;

[0030] (3) 1 bottle of standard negative serum, 1ml / bottle;

[0031] (4) 2 bottles of diluent, 10ml / bottle.

[0032] The shelf life of the kit is one year.

[0033] 1 Preparation of indirect hemagglutination antigen of Streptococcus suis

[0034] 1.1 Culture medium preparation

[0035] Prepare THB liquid medium 2000ml: 4.0g beef powder, 40g tryptone, 40g glucose, 6.0g sodium chloride, Na 2 HPO 4 5.8g, KH 2 PO 4 Add 0.6g, Yeast extracts 6.0g to deionized water, set the volume to 2000mL, fully stir to dissolve, and use anhydrous Na 2 CO 3 Adjust the pH to 7.4. Sterilize under high temperature and high pressure. After...

Embodiment 2

[0058] Apply the test kit of the present invention to detect the serum to be tested, the specific method is as follows:

[0059] First add 75 μL of diluent to each well of 8 rows and 12 columns of a 96-well “∨” polystyrene titration plate; In column 1, add 25 μL of standard positive serum and standard negative serum to the first well of columns 10 and 11, respectively, and column 12 is a blank control. Use a row gun to thoroughly mix wells 1-11, then pipette 25 μL into well 2, serially dilute to well 8, discard 25 μL from well 8 after mixing; finally add 1% sensitized red blood cells to each well Suspension (i.e. indirect haemagglutination diagnostic antigen of Streptococcus suis with a concentration of 100 μg / ml) 25 μL, after adding the sample, place the ∨-shaped titer plate on a micro shaker for 1-2 minutes, cover with a glass plate, and place at a constant temperature of 37 °C Box 1.5-2h, judge the result.

[0060] Judgment criteria: all erythrocytes agglutination (++++);...

Embodiment 3

[0061] Embodiment 3 The specificity test of Streptococcus suis indirect hemagglutination detection kit of the present invention

[0062] Use Streptococcus suis indirect hemagglutination detection kit of the present invention to detect healthy rabbit serum, Staphylococcus positive serum, Enterococcus faecalis positive serum, Enterococcus faecium positive serum, Streptococcus suis positive serum, the result except Streptococcus suis, The rest were negative (see Table 2), indicating that the antigen has a very high specificity.

[0063] Table 2 Specific test results of indirect hemagglutination diagnostic reagents for Streptococcus suis

[0064]

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Abstract

The invention provides a kit for detecting indirect hemagglutination of streptococcus suis. The kit comprises indirect hemagglutination antigen of streptococcus suis, and the indirect hemagglutination antigen of streptococcus suis is composed of whole bacterial protein of streptococcus suis CCTCC M 2016028 and aldehydated-tanned sheep red blood cell. The kit has the advantages that the operation is simple, convenient and rapid, no special equipment and apparatus is required, a whole course can be completed in 2-3 hours, the kit is suitable for basic popularization and application, and can be widely used for investigation and research of epidemiology of swine streptococcosis. The results have the advantages of good repeatability, stabilization, and reliablility, and can accurately detect the antibody of streptococcus suis.

Description

technical field [0001] The invention belongs to the technical field of detection reagents for microorganisms, and in particular relates to a detection kit for indirect hemagglutination of Streptococcus suis. Background technique [0002] Streptococcus suis ( Streptococcus. suis ) are Gram-positive facultative anaerobic cocci arranged in chains, without flagella, immobile, non-spore-forming, but capsulated. It is divided into 35 serotypes, of which type 2 has the strongest pathogenicity, the highest infection rate, and the widest distribution. The bacterium can cause symptoms such as meningitis and pneumonia in pigs and even death, as well as symptoms such as sepsis and endocarditis in humans and even death. Streptococcus suis has a high prevalence in pigs and is uncommon in humans, but the disease is very serious. The onset time is relatively concentrated in the high temperature season from June to August. Cases of human infection with Streptococcus suis were reported as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/86G01N33/569G01N33/531
CPCG01N33/531G01N33/56944G01N33/86
Inventor 周建华李学瑞马丽娜王立燕刘永安刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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