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Enterococcus hirae indirect hemagglutination antibody detection kit and preparation method thereof

A technology of Enterococcus haimani and a kit, which is applied in the field of microorganism detection reagents, can solve problems such as unreported, and achieve the effects of simple and convenient operation, low cost and economical benefits.

Inactive Publication Date: 2019-04-26
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are very few research reports on Enterococcus hirae at home and abroad. The detection technology for Enterococcus hirae mainly relies on laboratory bacterial isolation and PCR molecular biology identification. The serum antibody detection technology for Enterococcus hirae has no see the report

Method used

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  • Enterococcus hirae indirect hemagglutination antibody detection kit and preparation method thereof
  • Enterococcus hirae indirect hemagglutination antibody detection kit and preparation method thereof
  • Enterococcus hirae indirect hemagglutination antibody detection kit and preparation method thereof

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Embodiment 1

[0037] The indirect hemagglutination kit for detecting the serum antibody of Enterococcus hirai involved in the present invention includes:

[0038] 1 vial (5ml) of Enterococcus hirae indirect hemagglutination antigen, stored at 4°C;

[0039] 1) 1 bottle (0.5ml) of standard positive serum, frozen at -20°C;

[0040] 2) 1 bottle (0.5ml) of standard negative serum, frozen at -20°C;

[0041] 3) 3 bottles of diluent (5ml / bottle), stored at 4°C.

[0042] Enterococcus hirae CCTCC M 2018115 used to prepare the antigen is a field isolate and is kept in the State Key Laboratory of Livestock Disease Pathogen Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences.

[0043] The preparation method of various reagents in the kit of the present invention is:

[0044] 1. Indirect hemagglutination antigen

[0045] Enterococcus hirae CCTCC M 2018115 strain was enriched and cultured, ultrasonically crushed, and then centrifuged to obtain the supernatant, which ...

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Abstract

The invention disclose an indirect hemagglutination kit for detecting enterococcus hirae serum antibodies and a preparation method for preparing and using the kit. The kit comprises (1), enterococcushirae indirect hemagglutination antigens; (2), standard positive serums; (3), standard negative serums; (4), a diluting solution. The invention further provides a preparation and application method ofthe kit. The invention provides the enterococcus hirae indirect hemagglutination antibody detection kit and the application therefore. The kit has the advantages that the operation of the kit is simple and convenient, and the kit is extensively popularized and used in each base-level veterinarian related units and farms; the kit only needs simple instrument and consumable materials when a sampleis detected, the whole process can be completed within 2-3 hours, the result judgement is visualized, the repeatability is good, and the enterococcus hirae antibodies can be rapidly, stably and accurately detected.

Description

technical field [0001] The invention relates to an enterococcus hirae indirect hemagglutination antibody detection kit and a preparation method thereof, belonging to the technical field of detection reagents for microorganisms. Background technique [0002] Enterococcus hirae belongs to the genus Enterococcus and is a Gram-positive coccus widely distributed in terrestrial animals and poultry. It can easily inhibit the growth of chickens, cause animal pericarditis and infect humans. Sepsis occurs later, and at the same time, it often leads to severe drug resistance in animals. At present, there are few reports of Enterococcus hirae infection in humans. However, recent cases in the United States suggest that a life-threatening disease has developed in humans as a result of infection with Enterococcus hirae. The bacterial infection is associated with a variety of clinical symptoms, including endocarditis, acute pyelonephritis, acute cholecystitis, and spontaneous bacterial pe...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 陈启伟郑福英宫晓炜刘永生
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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