Detection method of actinobacillus pleuropneumoniae in porcine serum

Abstract

The invention relates to a detection method of actinobacillus pleuropneumoniae in porcine serum. The steps include: conducting random selection of scale farm piglets and fattening pig serum samples, firstly conducting IHA (indirect hemagglutination test) detection on the collected serum samples, then carrying out APP ELISA detection on the collected serum samples so as to be able to detect the actinobacillus pleuropneumoniae in porcine serum. The detection method provided by the invention employs combined use of APP indirect hemagglutination test and APP ELISA test, makes the actinobacillus pleuropneumoniae detection result faster and more accurate, reliable and sensitive, the detection result is stable and has no error. Also the detection method has the advantages of low cost and simplicity, is suitable for grass-roots application, and brings about great convenience to pig breeding, disease prevention and resisting.

Description

Technical field [0001] The invention belongs to the technical field of pig serum detection, in particular to a method for detecting Actinobacillus pleuropneumoniae in pig serum. Background technique [0002] Porcine infectious pleuropneumonia is a respiratory infectious disease caused by Actinobacillus pleuropneumoniae (APP). It is characterized by acute hemorrhagic fibrinous pleurisy and chronic fibrous necrotizing pneumonia. Since the disease was discovered in the United Kingdom in 1957, it has been widespread worldwide and has become one of the five major epidemics that have harmed the modern pig industry. Although the disease was discovered late in my country, it has serious harm to the breeding industry and has become one of the three most common respiratory diseases in the sick pig industry in my country. [0003] There are many existing detection methods for Actinobacillus pleuropneumoniae, which can be roughly divided into three categories, namely, pathogenic diagnostic me...

AI Technical Summary

Problems solved by technology

The etiological diagnosis method requires bacterial isolation, culture and identification, and the cycle is long; the serological diagnosis method includes complement fixation reaction, fluorescent antibody, indirect hemagglutination test and enzyme-linked immunosorbent assay (ELISA), etc. The disadvantages of the serological diagnosis method are It can only detect the antibody of Actinobacillus pleuropneumoniae in animals but cannot detect the pathogen; the molecular biology diagnosis method mainly includes reverse transcription polymerase chain reaction (RT-PCR) and fluorescent nucleic acid probe. This method is fast and sensitive, but requires the use of special instruments, such as PCR instruments, and the preparation of fluorescent probes is relatively expensive, which is not suitable for grassroots applications

[0004] However, sometimes when the above-mentioned detection methods are used alone for detection, the detection results are often unstable, and sometimes errors occur, which also brings great inconvenience to pig breeding, disease prevention, and disease resistance.

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

Images