Gosling plague viral vaccine

A technology of gosling plague virus and vaccine, which is applied in the direction of vaccines, viruses, antiviral agents, etc., can solve the problems such as the reduction of vaccine potency, achieve good safety, and prevent the effect of gosling plague virus infection in young Muscovy ducks

Active Publication Date: 2016-11-09
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, due to the reduction of the potency of the vaccine used due to virus mutation, it is also necessary to screen out the mutated virus strain to prepare a vaccine with higher efficacy

Method used

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  • Gosling plague viral vaccine
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  • Gosling plague viral vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, the screening of YBGPV-M strain

[0015] In 2013, a disease characterized by diarrhea and embolism due to the shedding of some duck intestinal mucosa appeared in a group of young muscovy ducks in a duck farm in Fujian Province. The incidence rate was 50% to 80%, and the fatality rate was 45% to 70%. The use of antibiotics, Muscovy duck parvovirus vaccine and Muscovy duck parvovirus hyperimmune yolk antibody could not control the disease. The inventor aseptically collected the liver, spleen and pancreas of dying ducks, homogenized the liver, spleen and pancreas with sterile normal saline to make a 20% suspension, centrifuged at 3000r / min for 15min, took the bacteria from the upper part and passed through the allantoic cavity Inoculate 11-day-old muscovy duck embryos, hatch for 168 hours, collect the allantoic fluid and embryo body tissue of the dead embryos after 24 hours, homogenize, freeze and thaw three times, and take the supernatant for freezing. Afte...

Embodiment 2

[0018] Embodiment 2 prepares vaccine

[0019] 1. Preparation of virus liquid for seedling production Dilute the YBGPV-M strain 100 times with sterile saline, inoculate 11-12 day-old susceptible Muscovy duck embryos in the allantoic cavity, 0.2ml per embryo, and incubate at 37°C , according to embryo inspection 2 times a day. Select duck embryos that died within 48 to 168 hours after inoculation, place them at 2 to 8°C for 4 to 12 hours, open the air chamber under sterile conditions, and pick out embryos with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantois For embryos with mild edema, take the embryo body (remove the head and limbs) and put it into a tissue grinder to crush, collect allantoic fluid and amniotic fluid, homogenate with embryo fluid, take the crushed tissue and add it at a ratio of 1:3 Physiological saline was mixed, freeze-thawed 3 times, centrifuged at 4000r / min for 30min, the supernatant was taken and mixed ...

Embodiment 3

[0043] 1. Preparation of antigens for seedling production

[0044] 1. Preparation of virus liquid for seedling production Dilute the YBGPV-M strain 100 times with sterile saline, inoculate 11-12 day-old susceptible Muscovy duck embryos in the allantoic cavity, 0.2ml per embryo, and incubate at 37°C , according to embryo inspection 2 times a day. Select duck embryos that died within 48 to 168 hours after inoculation, place them at 2 to 8°C for 4 to 12 hours, open the air chamber under sterile conditions, and pick out embryos with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantois For embryos with mild edema, take the embryo body (remove the head and limbs) and put it into a tissue grinder to crush, collect allantoic fluid and amniotic fluid, homogenate with embryo fluid, take the crushed tissue and add it at a ratio of 1:3 Physiological saline was mixed, freeze-thawed 3 times, centrifuged at 4000r / min for 30min, the supernatant...

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Abstract

The invention aims at providing a muscovy-duck-source gosling plague virus inactivated vaccine. The vaccine comprises an antigen and a vaccine adjuvant. The adopted antigen is an inactivated muscovy-duck-source gosling plague virus, and the preservation number of the adopted muscovy-duck-source gosling plague virus is CCTCC NO:V201620. A muscovy-duck-source gosling plague virus YBGPV-M strain with low toxicity and high immunogenicity is screened out and then inoculated with a duck embryo, the infected embryo and allantoic fluid are collected, after homogenization, ultrafiltration and concentration and formaldehyde solution inactivation, the oil adjuvant is added, and the vaccine is prepared after mixing and emulsifying. The prepared vaccine immunizes breeding muscovy ducks, the antibody level of the breeding muscovy ducks can be improved, the offspring maternal antibody level of the breeding muscovy ducks is guaranteed, and young muscovy duck gosling plague virus infection caused by the muscovy-duck-source gosling plague virus is prevented; antibodies can be produced after 10 days when 1-day young muscovy ducks are immunized, and young muscovy duck gosling plague virus infection caused by the muscovy-duck-source gosling plague virus can be effectively prevented. The prepared vaccine has the advantages of being efficient and good in safety.

Description

technical field [0001] The invention belongs to the technical field of pathogenic microorganism screening, and in particular relates to an inactivated vaccine of gosling plague virus. Background technique [0002] Goose parvovirus disease, also known as Derzsy's disease, commonly known as goose flu, goose or gosling plague, foie hepatitis, goose enteritis, etc., is a highly contagious disease that affects young geese and Muscovy ducks. The diversity of disease names reflects multiple pathological features of the disease. According to the age of infected goslings and ducklings, the disease can be manifested as acute, subacute and chronic. The acute type can cause 100% death of goslings within 10 days of age. Many countries have also reported a completely different antigenic Muscovy duck parvovirus infection with a mortality rate as high as 80%. The disease mainly occurs in 1 to 3-week-old gooses and young Muscovy ducks, especially those about 1 week old are more susceptibl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/23A61P31/20
CPCA61K39/12A61K2039/5252A61K2039/552C12N2750/14334Y02A50/30
Inventor 邹敏吴发兴宫晓王龙刘新文李芳范根成
Owner YEBIO BIOENG OF QINGDAO
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