Method for improving immunity of rabbits by combining bursin and vaccine

A cystin tripeptide and immunity technology, which can be used in tripeptide components, drug combinations, and resistance to vector-borne diseases, etc. Rabbit immunity is greatly affected and other problems, so as to make up for the blank period of immunity, without any side effects, and to enhance the effect of immunity

Inactive Publication Date: 2015-11-18
山东仙普爱瑞科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The rabbit immunization method of the prior art has the following defects: (1) the immunity of the rabbit is not greatly improved, and at the age of 65 days, the conversion rate of the rabbit plague antibody is low; (2) the body weight of the rabbit increases less; (3) ) Rabbit feed meat is relatively large
(4) Feed intake and water intake of sick animals are reduced, which has a greater impact on the immunity of sick rabbits, and the dosage and efficacy of the medicine are obviously insufficient
The traditional method of preventing and treating rabbit plague can achieve a certain immune effect, but as far as the current clinical application situation is concerned, there are still great deficiencies in the vaccine's action time and immune effect of the vaccine.

Method used

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  • Method for improving immunity of rabbits by combining bursin and vaccine
  • Method for improving immunity of rabbits by combining bursin and vaccine
  • Method for improving immunity of rabbits by combining bursin and vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 A clinical application method of combined injection of bursin tripeptide and rabbit plague vaccine

[0029] Step 1. Vaccine preparation

[0030] (1) Preparation of bursin tripeptide solution

[0031] Raw material: bursin tripeptide powder, purity 99.9%.

[0032] Preparation method: Add the above-mentioned vesicin tripeptide into distilled water to prepare a solution with a concentration of 0.1 g / ml.

[0033] (2) Preparation of mixed vaccine of bursin tripeptide and rabbit plague vaccine

[0034] All rabbit plague vaccines used in the present invention are purchased from: Beijing Lebeide Animal Husbandry Co., Ltd.

[0035] Add the bursa tripeptide solution prepared in step (1) into the rabbit plague vaccine, and the bursa tripeptide accounts for 0.1% of the mass percentage of the rabbit plague vaccine; turn the bursa tripeptide mixed upside down to make the rabbit plague vaccine It is fully mixed, then placed in a shaker with a rotating speed of 200r / min, ...

Embodiment 2

[0055] Example 2 A clinical application method of combined injection of bursin tripeptide and rabbit plague vaccine

[0056] Step 1. Vaccine preparation

[0057] Using the preparation method of Example 1, only changing the mass percentage of bursin tripeptide in the rabbit plague vaccine was used to carry out Examples 2-4.

[0058] See Table 1 for the mass percentage of the bursin tripeptide of Examples 2-4 in the rabbit plague vaccine:

[0059] Table 1

[0060]

[0061] Step 2. Vaccination

[0062] Using the vaccine injection method of Example 1, only changing the amount of bursin tripeptide per rabbit to carry out Example 2-4. See Table 2 for the dosage of each rabbit bursin tripeptide in embodiment 2-4:

[0063] Table 2

[0064]

[0065] Step 3, feeding

[0066] Adopt the raising method of embodiment 1.

Embodiment 1-4

[0067] Embodiment 1-4 experimental result:

[0068]When the rabbits were 25, 45 and 65 days old, blood was collected from the rabbits of Examples 1-4 and the control example, and the serum was separated, and the rabbit plague IgG antibody ELISA detection kit (purchased from Kunming Nuojin Technology Co., Ltd. ) to detect serum and calculate the positive rate, the operation method is:

[0069] (1) Add 100 μl negative and positive control samples to the negative control and positive control wells respectively;

[0070] (2) Take 100 μl of isolated rabbit serum from the rabbits in Examples 1-4 and the rabbit in the control example, dilute at 1:100, put them into the corresponding wells as samples to be tested, and make records;

[0071] (3) Incubate at 37°C for 1 hour;

[0072] (4) Discard the liquid in each well into the waste liquid bucket, and add 250 μl of washing solution to each well (the 20× concentrated washing solution in the kit must be diluted with distilled water o...

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Abstract

The invention provides a method for improving the immunity of rabbits by combining bursin and a vaccine. The method for improving the immunity of rabbits comprises vaccine injection and feeding, wherein the vaccine used during vaccine injection is a mixed vaccine composed of bursin and a rabbit plague vaccine, the bursin accounts for 0.1-0.8% of the rabbit plague vaccine in mass percentage, the vaccine injection comprises the first-time injection and the second-time injection, the first-time injection is carried out when the baby rabbit is 20 days old, the use amount of bursin for each baby rabbit is 0.003-0.01ml, the second-time injection is carried out when the baby rabbit is 40 days old, and the use amount of bursin for each baby rabbit is 0.006-0.02ml. The fodder used during feeding comprises wax gourd seed powder, lily root flour, photosynthetic bacteria preparation, bacillus thermoamylovorans preparation and basic forage. With the adoption of the method, the immunity of rabbits is obviously improved, the weight of the rabbits is obviously increased, and the feed conversion ratio is reduced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically, the invention relates to a method for improving rabbit immunity by using bursin tripeptide in combination with a vaccine. Background technique [0002] At present, the rabbit breeding industry generally adopts large-scale and intensive farming methods, which are prone to problems such as low immune function of rabbits and low vaccine immunity, and even the immunosuppressive effect of vaccines. The method of normal growth of breeding rabbits has become a top priority. Yet adopt prior art simple injection vaccine, although certain effect of strengthening immunity is arranged, its quality control but exists serious problem. [0003] Bursin tripeptide (bursin, BS) is an active tripeptide substance isolated from the bursa of Fabricius (bursaoffabricius, BF, which is the central lymphoid organ of humoral immunity unique to poultry), and its function is to induce poultry and mammal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61P31/14A61P37/04A61K38/06
CPCY02A50/30
Inventor 郭海岩刘刚王茂超王兴业张淑兰郭莎莎王雅静王洪军
Owner 山东仙普爱瑞科技股份有限公司
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