Compound plague vaccine and preparation method thereof

A plague vaccine and vaccine technology, which is applied in the field of plague vaccines, can solve the problems of short protection period, high side reaction rate, and difficulty in vaccine transportation, and achieve the effects of reducing side effects, improving safety, and improving immune effect
CN101337069BInactive Publication Date: 2012-07-25NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NAT INST FOR THE CONTROL OF PHARMA & BIOLOGICAL PROD
Publication Date
2012-07-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a composite plague vaccine and the preparation method thereof. The plague vaccine comprises thallus split products of the attenuated strain of plague bacilli and recombination plague bacilli V antigens, and further comprises plague bacilli F1 antigens, wherein, by collecting the thallus of plague attenuated strain grown on the culture medium, the thallus split products of the plague attenuated strain is obtained through the cracking preparation by high-pressure homogenization. Bacterial cell wall with good adjuvant effect, bacterial protein with antigenic specificity (which includes protective capsular antigen F1) and CpG fragments which are rich in bacterial DNA, with stronger immunological competence, are concentrated in the split products; the expression vector including plague bacilli V antigens or F1 antigens which are constructed by adopting the gene recombination technology is transformed into escherichia coli, and after the inducible expression is performed, recombination plague bacilli V antigens or F1 antigens are obtained through purification. The vaccine is applicable to the general population for immunizing and preventing the plague.
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Description

technical field

[0001] The invention relates to a plague vaccine used for the immune prevention of human or animal from Yersinia pestis infection, especially suitable for the immune prevention of normal people, and also relates to the preparation method of the vaccine. Specifically, the vaccine contains the cell lysate of the attenuated strain of Yersinia pestis and the Yersinia pestis V antigen, and optionally also includes the Yersinia pestis F1 antigen. Background technique

[0002] Plague is a flea-mediated zoonotic disease caused by the bacterium Yersinia pestis (also known as Yersinia pestis). There have been three plague pandemics in the world. In the second epidemic that broke out in Europe in 1348, 30% of the European population died. In past plague pandemics, hundreds of millions of people died of plague. Plague among humans is rare now, but plague among rats is still rampant. The natural foci of plague worldwide have not shrunk. In addition, economic difference...

Claims

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