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Goose parvovirus and applications thereof

A technology of goose parvovirus and goose plague virus, applied in the direction of antiviral agent, virus/bacteriophage, antiviral immunoglobulin, etc., can solve the problem of different gene sequences, achieve long duration, high antibody level, and good immunity original effect

Active Publication Date: 2014-01-22
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research data show that after years of evolution, although the serum of gosling plague virus has not changed significantly, the gene sequence is significantly different

Method used

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  • Goose parvovirus and applications thereof
  • Goose parvovirus and applications thereof
  • Goose parvovirus and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Obtaining and characteristic determination of goose parvovirus

[0029] 1. Virus Isolation

[0030] The goose liver tissue suspected to be infected with goose parvovirus was ground and homogenized to obtain a disease sample. Physiological saline with 1 / 5 times the volume of the disease material sample, and penicillin and streptomycin at a final concentration of 2000 units were added. Freezing and thawing were repeated 3 times, centrifuged at 7000 r / min for 20 min, and the supernatant was collected. Filter with a microporous membrane, take the filtrate and inoculate 10 12-14-day-old goose embryos through the allantoic cavity, discard the dead embryos within 24 hours; collect the dead embryos within 24-96 hours and take their allantoic fluid; within 96 hours of collection The liver tissue of the undead goose embryo was ground into a homogenate and then mixed with the above-mentioned allantoic fluid to form a suspension. The suspension was repeatedly frozen an...

Embodiment 2

[0048] Preparation and Application of Embodiment 2 Gosling Plague Virus Vaccine

[0049] 1 Virus culture

[0050] The virus seed (Example 1) was diluted 100 times with physiological saline, inoculated with 11-13 days old goose embryos, 0.2 mL per embryo, and cultured at 37°C. Goose embryos that died within 48 hours were discarded. When the culture time reaches 168 hours, the allantoic fluid and embryo body of dead goose embryos are collected. After removing the gallbladder, the embryo body is homogenized and added to the allantoic fluid, centrifuged at 5000r / min for 10min, and the supernatant is collected as the GPV-YZ strain virus liquid , stored at -20°C.

[0051] 2 Virus content

[0052] The GPV-YZ strain virus solution was diluted 10 times with normal saline, and inoculated into the chorioallantoic cavity of 11-13 day-old goose embryos, 0.2 mL per goose embryo, and used as a blank control. Five goose embryos were inoculated for each dilution, sealed with paraffin, and ...

Embodiment 3

[0083] Example 3 Preparation and application of anti-gosling plague egg yolk antibody

[0084] The gosling plague virus vaccine (GPV-YZ strain) prepared in Example 2 was used to prepare anti-gosling plague egg yolk antibodies.

[0085] 1. Immune laying hens

[0086]Healthy laying hens were used, and the chest muscles were injected with gosling plague virus vaccine at multiple points, 0.5ml / hen, which was the first immunization. Ten days after the first immunization, each laying hen was immunized with 1.0ml of gosling plague virus vaccine. 20 days after the first immunization, each laying hen was inoculated with 2.0ml of gosling plague virus vaccine. After three times of immunization, regularly check the titer of egg yolk agar diffusion test (the detection method refers to "2000 Chinese Veterinary Pharmacopoeia"), when the titer of agar reaches 2 5 , start collecting free eggs.

[0087] 2. Preparation of anti-gosling plague egg yolk antibody

[0088] Disinfect the collecte...

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Abstract

The invention provides goose parvovirus (GPV) and applications thereof, and belongs to the technical field of biology. The invention provides a goose parvovirus GPV-YZ strain as well as applications thereof in preparing medicaments for preventing and treating the gosling plague disease. The microorganism preservation number is CGMCC NO.8160. The invention also provides a gosling plague disease viral vaccine as well as an anti-gosling plague yolk antibody. The goose parvovirus YZ strain provided by the invention has favorable immunogenicity for the present epidemic goose parvovirus, can resist toxicity attacking of the GPV-AV240 strain, the GPV-GD strain as well as the toxic strain of the goose parvovirus, and has the protection capability against the GPV-AV240 strain and the GPV-GD strain. After breeding geese are immunized by the gosling plague disease viral vaccine provided by the invention, young geese can be protected by maternal antibodies, and can be prevented from being infected by gosling plague viruses. The anti-gosling plague yolk antibody provided by the invention can prevent and treat gosling disease.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to gosling plague virus and its application. Background technique [0002] Gosling plague is an acute or subacute septic infectious disease of goslings caused by goose parvovirus (also known as goose parvovirus, abbreviated as GPV). Gosling plague is the most severe infectious disease of goslings. Once goslings are infected, the case fatality rate is as high as 50-95%. Gosling plague virus is mainly transmitted through the secretions, excreta, breeding eggs of sick geese, and water, feed, utensils, and venues that sick geese have drunk. Clinical manifestations include mental fatigue, lone spouse, serous nasal discharge from the nostrils, frequent shaking of the head of the affected goose, loose grayish-yellow or yellow-green feces, nervous disorders, necrosis and shedding of the middle and posterior small intestine mucosa, and fibrinous exudate coagulation to form a thrombu...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/23A61K39/42A61P31/20C07K16/08C07K16/02C12R1/93
CPCY02A50/30
Inventor 卢宇邓碧华吕芳张金秋侯继波赵晓娟赵艳红
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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