Biological product containing novel goose parvovirus and duck circovirus antigen-antibody complex

A technology of goose parvovirus and duck circovirus, which is applied in the direction of virus antigen components, medical preparations containing active ingredients, viruses/bacteriophages, etc. It can solve the problems of slow efficacy of traditional Chinese medicine preparations, weak therapeutic effect, and increased feeding costs. , so as to achieve the effects of rapid immune effect, improved protective effect, and cost-saving prevention and treatment

Active Publication Date: 2022-06-24
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, traditional Chinese medicine preparations are slow in efficacy, large in dosage, unstable in quality, and inconvenient to use; yolk antibody has a weak therapeutic effect on mixed infection and secondary infection, and as the age of the diseased duck increases, yolk antibody It is difficult to protect the whole growth process of meat ducks after injection, and repeated injections will increase the cost of feeding; simple live vaccines have the possibility of strong virulence, potential infectiousness and pathogenicity, and the vaccine interacts with maternal antibodies The problem of inoculation time and method may affect the final immune effect

Method used

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  • Biological product containing novel goose parvovirus and duck circovirus antigen-antibody complex
  • Biological product containing novel goose parvovirus and duck circovirus antigen-antibody complex
  • Biological product containing novel goose parvovirus and duck circovirus antigen-antibody complex

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: the preparation of new goose parvovirus live vaccine and inactivated vaccine

[0026] 1. The disease material of the present invention is collected from the viscera and duck feather hair follicles of Cherry Valley meat duck with duck short beak dwarfism syndrome and duck feather shedding syndrome in Cherry Valley duck farm in Anhui Province. Using the gene sequence of the classic goose plague virus (SYG61v strain) in GenBank (GenBank sequence number: KC996729), use Primer5.0 software to design upstream and downstream primers (F: 5'-GTAAGGGGTCTGTTAGTATTG-3'; R: 5'-GCTGTTGGTCGTAGTAGGC-3 '). After the diseased material was ground, the supernatant was extracted, etc., PCR amplification and sequencing were carried out with the above primers, and it was confirmed to be a new type of goose parvovirus.

[0027] 2. The virus was identified by PCR of various waterfowl viruses (including duck circovirus, duck plague, adenovirus, avian influenza virus, duck Tembusu v...

Embodiment 2

[0039] Embodiment 2: Preparation of novel goose parvovirus egg yolk antibody IgY

[0040] (1) Preparation of highly immune eggs: immunize laying hens with new goose parvovirus live vaccine and inactivated vaccine successively, carry out live vaccine immunization at the young age of laying hens (25-35 days old), and carry out 80-90 days later For the first inactivated vaccine immunization, the interval is 21 days, and the inactivated vaccine is used for the second immunization, and after another 21 days, the inactivated vaccine is used for the third immunization; 14 days after the third immunization of the inactivated vaccine, the high-immune eggs are collected, And carry out the two-way diffusion test of agar expansion, and collect the high-immune eggs with the agar expansion titer ≥ 1:64.

[0041] (2) Extraction and preparation of egg yolk antibody IgY: immerse high-immune eggs in 1% bromogeramine solution, soak and sterilize for 15 minutes, and dry for later use; mechanicall...

Embodiment 3

[0042] Example 3: Preparation of duck circovirus Cap protein antigen

[0043] Using the co-conserved fragment of the Cap protein gene of the duck circovirus classic strain (GeneBank sequence number: GU014543.1) in GenBank, use the software Premier 5.0 to design and synthesize the upstream and downstream primers of the Cap protein gene; take the diseased tissue, grind and homogenize The total RNA was extracted by conventional RNA extraction technology and reverse-transcribed into cDNA; using the cDNA as a template, the Cap protein gene was amplified and recovered by using the upstream and downstream primers of the Cap protein gene; the recovered product and the expression vector used used the same restriction Endonucleases are digested and ligated, and the ligated products are transformed into expression vectors; positive clones are screened for culture and induction, and the expression cells are recovered; the cells are ultrasonically disrupted and obtained after affinity chrom...

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Abstract

The invention provides a biological product containing a novel goose parvovirus and duck circovirus antigen-antibody compound. The biological product is prepared by the following steps: firstly, preparing an antigen-antibody compound from a novel goose parvovirus egg yolk antibody and a live vaccine thereof; preparing an antigen-antibody complex from the duck circovirus egg yolk antibody and duck circovirus Cap protein; and mixing the two antigen-antibody complexes, and adding a protective agent to finally form a biological product. The biological product can resist invasion of the novel goose parvovirus and the duck circovirus to an organism at the same time, and has good prevention and protection effects on the duck short beak and dwarf syndrome and the duck defeathering syndrome caused by the goose parvovirus and the duck circovirus.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, in particular to a biological product containing a novel goose parvovirus and duck circovirus antigen-antibody complex. Background technique [0002] The main symptom of "Feathers Shedding Syndrome" (Feathers Shedding Syndrome, FSS) is that the whole body feathers fall off partially or completely, and the feathers are difficult to pull out after slaughter (burr ducks), and the spirit is listless. The body is small and the body weight is light. After anatomy, the main symptoms are thymus hemorrhage, partial brain hemorrhage, heart, liver and spleen with bleeding points, intestinal edema, etc. The disease has no obvious age of onset, and the course of the disease is long. It is a disease of "Short beak and dwarfism syndrome" (SBDS) accompanied by growth retardation, atrophy and shortening of the upper and lower beaks, and tongue exposure. Studies have confirmed that the occurrence of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/42A61K39/12A61K39/23A61P31/20
CPCA61K39/42A61K39/12A61P31/20A61K2039/552A61K2039/70C12N2750/14334C12N2750/10034Y02A50/30
Inventor 李守军刘冠星车艳杰张永欣杨燚关世鑫刘洋李睿刘海霞
Owner TIANJIN RINGPU BIO TECH
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