36results about How to "Improve the effectiveness of protection" patented technology

The present invention relates to a streptococcus antigen C5a and a preparation method thereof. The streptococcus protective antigen C5a is an SEZC5a recombinant protein, which consists of 571 amino acids and has a molecular weight of 60.3kDa; a forward primer has one BamHI restriction enzyme cutting site, and a reverse primer has one EcoRI restriction enzyme cutting site. According to the preparation method of the streptococcus protective antigen C5a, the SEZ C5a recombinant protein is treated with cloning, expression and purification; and a series of biological engineering technologies and experiments on mice are applied to conduct system analysis on an rSCPZ. After vaccination, the rSCPZ can provide high protective efficacy; an anti-rSCPZ mice double-immunized serum has significant passive immune protection on mice; and the mice immunized by the rSCPZ show high level of antibody titer in serum. The anti-rSCPZ antibody can induce high level of bactericidal capability; an scpZ gene has a transcription level in SEZ (Streptococcus zooepidemicus) infected mice higher than that of culture in vitro; and the rSCPZ can adhere to hep-2 cells and inhibit cell infection ability of SEZ.

The invention discloses a preparation method and an application of a nanocomposite with functional molecules coated with an erythrocyte membrane. The nanocomposite comprises an erythrocyte membrane and functional molecules, wherein the functional molecules are mRNA, protein and small molecule drugs. According to the invention, the modified erythrocyte is prepared, the erythrocyte membrane coated nucleic acid drug is extracted, lipid components such as erythrocyte membrane phospholipid can make a functional drug carrier efficiently target the spleen, and the drug is delivered into the spleen or liver cells and released to play a therapeutic role; in addition, the cell membrane improves the in-vivo circulation stability and the intracellular delivery efficiency of the functional medicine. The nanocomposite is simple in preparation and extraction method, suitable for large-scale synthesis and preparation, mild in preparation condition, high in efficiency, uniform in particle size, good in dispersity, good in targeting capacity, high in biocompatibility and efficient and stable in intracellular delivery efficiency.

The present invention relates to a safety guardrail for highway construction. The safety guardrail comprises a rectangular frame and a steel wire net arranged on the front side of the frame, a plurality of buffering assemblies are horizontally and evenly distributed on the frame, and each buffering assembly is formed by universally hinging two rollers which are coaxially arranged up and down; a plurality of guide rods in one-to-one correspondence with the buffering assemblies are arranged on the frame, the upper ends of the guide rods are vertically connected with the frame in a sliding mode and connected with the frame through first springs, the guide rods slide upwards under the elastic force of the first springs, and the lower ends of the guide rods are universally hinged to the upper ends of the buffering assemblies; a plurality of positioning rods in one-to-one correspondence with the buffering assemblies are arranged on the frame, the lower ends of the positioning rods are rotationally connected with the frame, and the upper ends of the positioning rods are universally hinged to the lower ends of the buffering assemblies. Impact force in all directions can be effectively decomposed, buffered and resisted in a multiple mode, the protection effectiveness is greatly improved, the overall service life of the guardrail is prolonged, the guardrail can be recycled, and unnecessary material and labor cost is reduced.

The invention discloses a nucleic acid and recombinant protein co-immune vaccine based on a classical swine fever virus gene as well as a preparation method and application of the co-immune vaccine. The preparation method comprises the steps that firstly, optimized recombinant plasmids and recombinant classical swine fever vaccine antigen protein are obtained by combining gene engineering and cellengineering; then the nucleic acid sequence and the recombinant protein are compounded into different liquid phase layers of a biphasic adjuvant, so that the obtained co-immune vaccine is good in immunogenicity and short in immune blank period, can rapidly induce an organism to generate high-level, high-affinity and durable antibody response and immune memory, effectively protects a target animalfrom being attacked by classical swine fever virulent viruses, and the method can be applied to preparation of products for preventing/treating classical swine fever.

The invention adopts a monoclonal antibody 5D9 with neutralization activity to both PRRSV-I and PRRSV-II viruses to prepare an immunoenhancer. When a formed immune complex is combined with an adjuvantto immunize mice, T cells secreted by IFN-gamma are significantly increased, suggesting that combined immunization of the PRRSV specific antibody 5D9 and the normal oil in water adjuvant can enhanceCTL reaction in the process of inactivating virus immunity. Animal tests show that the immune protection rate of the immune complex prepared by the method can achieve a higher protection effect than that of a vaccine and a commercialized attenuated vaccine prepared only with a commercial adjuvant ISA 206.

The invention provides a biological product containing a novel goose parvovirus and duck circovirus antigen-antibody compound. The biological product is prepared by the following steps: firstly, preparing an antigen-antibody compound from a novel goose parvovirus egg yolk antibody and a live vaccine thereof; preparing an antigen-antibody complex from the duck circovirus egg yolk antibody and duck circovirus Cap protein; and mixing the two antigen-antibody complexes, and adding a protective agent to finally form a biological product. The biological product can resist invasion of the novel goose parvovirus and the duck circovirus to an organism at the same time, and has good prevention and protection effects on the duck short beak and dwarf syndrome and the duck defeathering syndrome caused by the goose parvovirus and the duck circovirus.

The invention discloses a man-machine interaction application method in the cryptology field based on a contact connection control instruction, and the method comprises the following steps: 1, loggingin a platform through a network, registering an account according to a rule, and getting unique terminal equipment as an account, and enabling the unique terminal equipment to serve as a unique loginaccount and password input equipment; 2, starting a user 'unique terminal device' to connect an intelligent device or a computer, and opening a platform through a network to carry out account verification and password input; 3, setting a password according to a custom password design rule, and performing multilayer grouping conversion on the password by a background through prompt operation of aplatform and a display screen; the number of the point locations of the language logic can be any number, the protection effect of the password is greatly improved, the language logic language characters can use any language characters as the basis, and new artificial symbols are used for improving the password strength, so that the risk that the password is decoded is reduced while the requirement of customizing the password by a client is met, and the use of the user is facilitated.

The invention provides a protection method based on mouth and nose respective processing and a device based on the protection method. According to the protection method, the mouth and the nose of a human face are used as independent areas for coverage protection. The two independent areas are isolated from each other, completely sealed or partially sealed. The mouth and the nose are respectively protected, the nose filtering material can be selected according to different conditions, the protection requirements under various different conditions are met, for example, the efficient nose filtering material can be considered to be selected in hospital wards and the like, so that the protection effect much higher than that of an existing mask can be obtained, and in general occasions, a commontextile can be selected, and the same protection effect as an existing mask can be obtained even if the nose protection cover is not used. In addition, the device also has good comfort and sanitation, can be recycled for a long time, and is environment-friendly and economical.