36results about How to "Improve the effectiveness of protection" patented technology

An armored protective sport glove for use in historical-type armed combat having an underlying durable flexible glove with extending cuff having rivets and washers. A contoured armored backplate with padded underlayment is attached to the underlying glove, the backplate connecting with underlying material strips which over and along the back of the fingers and thumb providing connections for overlapping finger and thumb plates which flare at the knuckles and wrap exposed portions of the hand and thumb. The contoured overlapping plates and underlying material connection strips connected with one another and to the underlying glove at specific positions allowing for increased mobility and ease of replacement of worn or broken materials. Fingertip protection is increased by use of soft durable connection means decreasing the risk of smashed or pinched fingertips. Pivot points at the wrist increase mobility at the wrist and thumb.

The present invention relates to a streptococcus antigen C5a and a preparation method thereof. The streptococcus protective antigen C5a is an SEZC5a recombinant protein, which consists of 571 amino acids and has a molecular weight of 60.3kDa; a forward primer has one BamHI restriction enzyme cutting site, and a reverse primer has one EcoRI restriction enzyme cutting site. According to the preparation method of the streptococcus protective antigen C5a, the SEZ C5a recombinant protein is treated with cloning, expression and purification; and a series of biological engineering technologies and experiments on mice are applied to conduct system analysis on an rSCPZ. After vaccination, the rSCPZ can provide high protective efficacy; an anti-rSCPZ mice double-immunized serum has significant passive immune protection on mice; and the mice immunized by the rSCPZ show high level of antibody titer in serum. The anti-rSCPZ antibody can induce high level of bactericidal capability; an scpZ gene has a transcription level in SEZ (Streptococcus zooepidemicus) infected mice higher than that of culture in vitro; and the rSCPZ can adhere to hep-2 cells and inhibit cell infection ability of SEZ.

The present invention provides the preparation process of recombinant type I and II goose interferon. The preparation process includes: RT-PCR amplifying the gene coding goose IFN-alpha mature protein and IFN-gamma mature protein; sequencing identification of gene sequences, directionally sub-cloning target genes to pET30a prokaryotic expression vactor separately; inducing expressing in colibacillus BL21(DE3)plysS separately, separately performing affinity chromatographic purification of the fusion expressed target protein, separately directionally sub-cloning to pBlueBacHis2A and pMel BacA baculovirus transfer vector, separately cotransfecting insect cell Sf9 with the transfer vector and linearized baculovirus DNA, recombining virus infected insect cell, and expressing goose mIFN-alpha and mIFN-gamma in the prokaryotic expression vactor. The products may be used as goose antiviral vaccine medicine and immunity adjuvant.

A multifunctional fence stand column comprises a cylinder and a warning belt and is characterized in that a hollow barrel serves as the cylinder, a rotating shaft part is arranged on the top of the cylinder, an infrared sound production alarm device is arranged in the middle of the rotating shaft part, the bottom end of the rotating shaft part is located in the cylinder and provided with a sector gear, the angle of the sector gear is an obtuse angle, the sector gear is meshed with a drive gear, the drive gear is connected with a power device, a top support is fixedly connected with the cylinder and provided with a solar electric generation device and a photosensor, an electrical control device is arranged in the cylinder, a light emitting device is arranged on the top support, a rechargeable storage battery is arranged in the cylinder, a push type power switch is arranged in the cylinder, a threaded plug cock is assembled on the side wall of the cylinder, the end, used for triggering the push type power switch, on the threaded plug cock is located in the cylinder, and the other end of the threaded plug cock is provided with a prism-shaped counter bore. The fence stand column can enhance the protection prompting function and improve protection effectiveness.

The invention discloses a preparation method and an application of a nanocomposite with functional molecules coated with an erythrocyte membrane. The nanocomposite comprises an erythrocyte membrane and functional molecules, wherein the functional molecules are mRNA, protein and small molecule drugs. According to the invention, the modified erythrocyte is prepared, the erythrocyte membrane coated nucleic acid drug is extracted, lipid components such as erythrocyte membrane phospholipid can make a functional drug carrier efficiently target the spleen, and the drug is delivered into the spleen or liver cells and released to play a therapeutic role; in addition, the cell membrane improves the in-vivo circulation stability and the intracellular delivery efficiency of the functional medicine. The nanocomposite is simple in preparation and extraction method, suitable for large-scale synthesis and preparation, mild in preparation condition, high in efficiency, uniform in particle size, good in dispersity, good in targeting capacity, high in biocompatibility and efficient and stable in intracellular delivery efficiency.

The invention relates to Streptococcus zooepidemicus protective antigen Sec_205 and a preparation method thereof. The Streptococcus zooepidemicus protective antigen Sec_205 is rSec_205. The antigen preparation method comprises cloning, conversion, inducible expression and purifying. The rSec_205 and an SEZ (Streptococcus zooepidemicus) rehabilitation pig serum show good immune responses; and the mice immunized by the recombinant protein detects high level of antibody titer in mice serum. mice second immune serum has obvious passive immune protection to mice, and an anti-rSec_205 antibody can induce high-level sterilization capability. The immunity protective tests show that after the mice is immunized by the rSec_205, high protection effectiveness is provided. The transcription level of a gene for coding the Sec_205 protein in SEZ-infected mice body is obviously higher than the transcription level of in vitro culture, a flow cytometry result shows that the Sec_205 is distributed on the surface of SEZ cell, which is cell surface protein, in a SEZ adhesion test, Sec_205 can obviously reduce the SEZ adhesion capability for cells, and the inhibition rate is 30.3%.

The present invention relates to a safety guardrail for highway construction. The safety guardrail comprises a rectangular frame and a steel wire net arranged on the front side of the frame, a plurality of buffering assemblies are horizontally and evenly distributed on the frame, and each buffering assembly is formed by universally hinging two rollers which are coaxially arranged up and down; a plurality of guide rods in one-to-one correspondence with the buffering assemblies are arranged on the frame, the upper ends of the guide rods are vertically connected with the frame in a sliding mode and connected with the frame through first springs, the guide rods slide upwards under the elastic force of the first springs, and the lower ends of the guide rods are universally hinged to the upper ends of the buffering assemblies; a plurality of positioning rods in one-to-one correspondence with the buffering assemblies are arranged on the frame, the lower ends of the positioning rods are rotationally connected with the frame, and the upper ends of the positioning rods are universally hinged to the lower ends of the buffering assemblies. Impact force in all directions can be effectively decomposed, buffered and resisted in a multiple mode, the protection effectiveness is greatly improved, the overall service life of the guardrail is prolonged, the guardrail can be recycled, and unnecessary material and labor cost is reduced.

The invention relates to a streptococcus equi subsp. zooepidemicus (SEZ) protective antigen HP0623 and A preparation method thereof. The SEZ protective antigen HP0623 is a SEZ HP0623 recombinant protein, is composed of 429 amino acid residues, and has a molecular weight of 47.04 kDa. The preparation method comprises steps of gene clone, enzyme cutting and enzyme linking, inducible expression, and protein purification. The HP0623 can be used to prepare vaccines. The provided SEZ protective antigen HP0623 expresses good immunogenicity in mouse experiments; the generated antibody can provide a strong protective force and induce a high level sterilizing performance; the SEZ protective antigen HP0623 is located on the surface of SEZ, and rHP0623 can inhibit the adhesion of SEZ on Hep-2 cells.

The invention relates to a sheep aphthosis virus fusion protein rB2LcF1L. An amino acid sequence of the sheep aphthosis virus B2L and F1L fusion protein is SEQ ID No:2; compared with the wild type sheep aphthous virus F1L protein, the sheep aphthosis virus fusion protein rB2LcF1L has amino acid sequences of sites No.1 to 70 deleted to facilitate the expression of the fusion protein and increase the expression level. At the same time, 6 groups of histidine tags are added to the C-terminus of the fusion protein to facilitate purification in subsequent production. In the above amino acid sequence, compared with a B2L amino acid sequence of a conventional isolate, the V / L of the amino acid at a site No.9 is mutated to G, namely ''IPVG or IPLG is mutated to IPGG'', and the LDCF at a site No.35is mutated to LYCF. The above mutation significantly increases the expression level and immunogenicity of the fusion protein.

The invention provides a recombinant duck viral enteritis virus, a preparation method and applications, belonging to the field of animal medicine vaccines. The avian influenza virus haemagglutinin gene is 1437th to 3131th nucleotide with the nucleotide sequence of SEQ ID NO:1. According to the recombinant duck viral enteritis virus, an expression cassette containing the avian influenza virus haemagglutinin gene is inserted in a spacer region between LORF11 and UL55 genes of duck viral enteritis virus genome. As a vaccine active ingredient, the avian influenza virus haemagglutinin gene can achieve better cross protection effect. The recombinant duck viral enteritis virus can stably and efficiently express the avian influenza virus haemagglutinin gene, virus is stable and grows well, the prepared live vaccine has higher protective efficacy on duck plague and avian influenza, higher avian influenza antibody is generated, and the duration period of the antibody is long.

The invention discloses a nucleic acid and recombinant protein co-immune vaccine based on a classical swine fever virus gene as well as a preparation method and application of the co-immune vaccine. The preparation method comprises the steps that firstly, optimized recombinant plasmids and recombinant classical swine fever vaccine antigen protein are obtained by combining gene engineering and cellengineering; then the nucleic acid sequence and the recombinant protein are compounded into different liquid phase layers of a biphasic adjuvant, so that the obtained co-immune vaccine is good in immunogenicity and short in immune blank period, can rapidly induce an organism to generate high-level, high-affinity and durable antibody response and immune memory, effectively protects a target animalfrom being attacked by classical swine fever virulent viruses, and the method can be applied to preparation of products for preventing / treating classical swine fever.

The invention adopts a monoclonal antibody 5D9 with neutralization activity to both PRRSV-I and PRRSV-II viruses to prepare an immunoenhancer. When a formed immune complex is combined with an adjuvantto immunize mice, T cells secreted by IFN-gamma are significantly increased, suggesting that combined immunization of the PRRSV specific antibody 5D9 and the normal oil in water adjuvant can enhanceCTL reaction in the process of inactivating virus immunity. Animal tests show that the immune protection rate of the immune complex prepared by the method can achieve a higher protection effect than that of a vaccine and a commercialized attenuated vaccine prepared only with a commercial adjuvant ISA 206.

The invention relates to an oxygen carrying coronary contrast medium, which comprises a contrast medium and a hemoglobin oxygen carrying carrier. According to the condition whether the contrast medium is mixed with the hemoglobin oxygen carrying carrier or not before using, the oxygen carrying coronary contrast medium has the following two types of: 1, storing the hemoglobin oxygen carrying carrier and the contrast medium respectively, wherein the hemoglobin oxygen carrying carrier is contained in dilution solution which is diluted by a diluent until the hemoglobin concentration is between 0.1 and 5g / dL, the pH value of the dilution solution is between 7.0 and 7.4; and making the hemoglobin oxygen carrying carrier contained in the dilution solution in an oxygen saturation state by oxygenating before using; and 2, forming mixed liquor by using the hemoglobin oxygen carrying carrier and the contrast medium, wherein the proportion of the contrast medium to the hemoglobin oxygen carrying carrier is limited by the hemoglobin concentration which is between 0.1 and 5 g / dL in the mixed liquor, and the pH value of the mixed liquor is between 7.0 and 7.4; and making the hemoglobin oxygen carrying carrier contained in the mixed liquor in the oxygen saturation state by oxygenating before using.

The invention discloses a waterproof box for storage of a photoelectric sensor. The waterproof box comprises a rubber ball and a rectangular opening formed inside the rubber ball, two mounting grooves communicating with the rectangular opening are symmetrically formed in the outer surface of the rubber ball, a box body is movably inserted into the rectangular opening, each of the left end and the right end of the box body is provided with a plugging base in a clamped mode, the two plugging bases are matched with the two mounting grooves, and iron sheets are arranged on the side edges of the plugging bases in the circumferential direction. According to the waterproof box for storage of the photoelectric sensor, through the arrangement of a locking part, namely the secondary safety part, inside the box body, the box body can be prevented from being cracked due to stress impact, a layer of protection is added on the basis of a conventional lock catch, then the box body is inserted into the rectangular opening, the two plugging bases are arranged at the left end and the right end of the box body in a sleeving manner, the two plugging bases are stabilized in the two mounting grooves outside the rubber ball through the magnetic attraction effect, and in this way, the isolation and protection effects of the photoelectric sensor in the box body can be further improved through the damping and protection effects of the rubber ball.

The present invention relates to a streptococcus antigen C5a and a preparation method thereof. The streptococcus protective antigen C5a is an SEZC5a recombinant protein, which consists of 571 amino acids and has a molecular weight of 60.3kDa; a forward primer has one BamHI restriction enzyme cutting site, and a reverse primer has one EcoRI restriction enzyme cutting site. According to the preparation method of the streptococcus protective antigen C5a, the SEZ C5a recombinant protein is treated with cloning, expression and purification; and a series of biological engineering technologies and experiments on mice are applied to conduct system analysis on an rSCPZ. After vaccination, the rSCPZ can provide high protective efficacy; an anti-rSCPZ mice double-immunized serum has significant passive immune protection on mice; and the mice immunized by the rSCPZ show high level of antibody titer in serum. The anti-rSCPZ antibody can induce high level of bactericidal capability; an scpZ gene has a transcription level in SEZ (Streptococcus zooepidemicus) infected mice higher than that of culture in vitro; and the rSCPZ can adhere to hep-2 cells and inhibit cell infection ability of SEZ.

The invention discloses a database event association method and a database auditing system using the same, and the method comprises the following steps: firstly carrying out the primary association ofa first event from a client to a Web server and a second event from the Web server to the database server according to an occurrence time window; judging whether the second event contains the effective value in the first event, performing secondary association, screening according to the occurrence time sequence, obtaining the final associated event, performing content auditing on the finally confirmed associated event, so that a real user of database operation can be accurately positioned, and database protection effectiveness is improved.

The invention discloses a detecting system and a detecting method for a safety testing mode. The detecting system is used for preventing entering the safety testing mode after a chip testing circuit is abandoned. The detecting system for the safety testing mode comprises a time sequence logic control module, a detecting circuit module and a triggering module. The triggering module is used for generating a high-level or low-level triggering signal according to an on-off state. The time sequence logic control module outputs a high-level testing mode signal according to the low-level triggering signal, thereby making a chip enter the safety testing mode, or the time sequence logic control module outputs a low-level testing mode signal according to the high-level triggering signal, thereby making the detecting circuit module output a self-destroying signal for performing a self-destroying operation. Therefore the detecting system for the safety testing mode improves protecting effectiveness for chip sensitive information and accords with a majority of technological realizing requirements. The detecting system can be widely applied to various safety chips.

The invention provides a biological product containing a novel goose parvovirus and duck circovirus antigen-antibody compound. The biological product is prepared by the following steps: firstly, preparing an antigen-antibody compound from a novel goose parvovirus egg yolk antibody and a live vaccine thereof; preparing an antigen-antibody complex from the duck circovirus egg yolk antibody and duck circovirus Cap protein; and mixing the two antigen-antibody complexes, and adding a protective agent to finally form a biological product. The biological product can resist invasion of the novel goose parvovirus and the duck circovirus to an organism at the same time, and has good prevention and protection effects on the duck short beak and dwarf syndrome and the duck defeathering syndrome caused by the goose parvovirus and the duck circovirus.

The invention discloses a light-color insulating wear-resistant styrene-butadiene rubber composition for a water-cooled cable sheath. The light-color insulating wear-resistant styrene-butadiene rubber composition comprises the following components in parts by weight: 70-100 parts of styrene-butadiene rubber, 0-30 parts of natural rubber, 2-5 parts of zinc oxide, 1-3 parts of stearic acid, 40-60 parts of white carbon black, 5-15 parts of silica powder, 40-60 parts of mica powder, 2-4 parts of a silane coupling agent, 5-15 parts of naphthenic oil, 2-4 parts of an anti-aging agent, 1-3 parts of protective wax, 0-1 part of sulfur and 3-6 parts of an accelerant. The butadiene styrene rubber product prepared by the invention not only has excellent physical and mechanical properties, but also has insulation, wear resistance and electric breakdown resistance, meets the requirements of main engine plants on the product, and has very high economic value.

The invention discloses a man-machine interaction application method in the cryptology field based on a contact connection control instruction, and the method comprises the following steps: 1, loggingin a platform through a network, registering an account according to a rule, and getting unique terminal equipment as an account, and enabling the unique terminal equipment to serve as a unique loginaccount and password input equipment; 2, starting a user 'unique terminal device' to connect an intelligent device or a computer, and opening a platform through a network to carry out account verification and password input; 3, setting a password according to a custom password design rule, and performing multilayer grouping conversion on the password by a background through prompt operation of aplatform and a display screen; the number of the point locations of the language logic can be any number, the protection effect of the password is greatly improved, the language logic language characters can use any language characters as the basis, and new artificial symbols are used for improving the password strength, so that the risk that the password is decoded is reduced while the requirement of customizing the password by a client is met, and the use of the user is facilitated.

An embodiment of the present invention provides a network routing protocol protection method based on traffic behavior. The method includes: the security management platform sends the rule template to the firewall; the firewall monitors the threat of the flow data according to the rule template and reports the threat flow data packets obtained through monitoring to the security management platform; the threat flow data received by the security management platform After the packet reaches the first threshold, an instruction is issued to enable the firewall to count the number of identical times of the most threatening routing protocol field parameters; The management platform generates a new configuration template. Compared with traditional routing protocol protection methods, the invention can effectively improve the protection effectiveness.

The invention discloses a superplastic stone blocking net. The superplastic stone blocking net comprises steel wire nets, steel columns, steel column bases, anchor rods and anchor rod bases; the steel column bases and the anchor rod bases are fixed on a foundation; a first end of every steel column is connected with the corresponding steel column base in a rotatable mode; the anchor rods are formed by superplastic materials; a first end of every anchor rod is connected with the corresponding steel column in a rotatable mode; a second end of every anchor rod is connected with the corresponding anchor rod base in a rotatable mode; the steel wire nets are formed by superplastic materials; the bilateral sides of every steel wire net are connected with the two adjacent steel columns. According to the superplastic stone blocking net, a decompressor or decompression ring device of a passive side slope protection system is omitted, the force transfer path of the stone blocking net is changed through the design of rotatable structures of the steel columns and the steel column bases, the anchor rods and the steel columns and the anchor rods and the anchor rod bases, and accordingly concentrated loads are effectively dispersed into different stress units and accordingly the cracking of the steel wire nets under the large load impact is effectively prevented and the protection effect is greatly improved.

The invention relates to a streptococcus equi subsp. zooepidemicus (SEZ) protective antigen HP0623 and A preparation method thereof. The SEZ protective antigen HP0623 is a SEZ HP0623 recombinant protein, is composed of 429 amino acid residues, and has a molecular weight of 47.04 kDa. The preparation method comprises steps of gene clone, enzyme cutting and enzyme linking, inducible expression, and protein purification. The HP0623 can be used to prepare vaccines. The provided SEZ protective antigen HP0623 expresses good immunogenicity in mouse experiments; the generated antibody can provide a strong protective force and induce a high level sterilizing performance; the SEZ protective antigen HP0623 is located on the surface of SEZ, and rHP0623 can inhibit the adhesion of SEZ on Hep-2 cells.

The invention provides a protection method based on mouth and nose respective processing and a device based on the protection method. According to the protection method, the mouth and the nose of a human face are used as independent areas for coverage protection. The two independent areas are isolated from each other, completely sealed or partially sealed. The mouth and the nose are respectively protected, the nose filtering material can be selected according to different conditions, the protection requirements under various different conditions are met, for example, the efficient nose filtering material can be considered to be selected in hospital wards and the like, so that the protection effect much higher than that of an existing mask can be obtained, and in general occasions, a commontextile can be selected, and the same protection effect as an existing mask can be obtained even if the nose protection cover is not used. In addition, the device also has good comfort and sanitation, can be recycled for a long time, and is environment-friendly and economical.

The invention discloses a safety belt system, a safety belt control method, a vehicle and a readable storage medium. The safety belt system comprises a webbing, a locking tongue, a lower end piece, a seatbelt retractor and a lock buckle, and the locking tongue is movably arranged on the webbing upper; the lower end piece is connected with one end of the webbing; the seat belt retractor includes a coil spring assembly and a movable guide sleeve assembly, and the end of the webbing away from the lower end sheet is connected with the reel of the coil spring assembly through the webbing channel opening of the seat belt retractor, and is movable. The guide sleeve assembly controls the tightening force of the webbing; the lock catch is engaged with the lock tongue. The invention can improve the wearing comfort of the safety belt under the condition of ensuring the protection effect of the safety belt as much as possible.

The invention belongs to the technical field of nuclear power maintenance and particularly relates to a mounting and fixing device of a nuclear power station water discharging port flashboard guide groove auxiliary anode. In the embodiment of the invention, a placing cylinder is connected with a pedestal; the pedestal can fix the placing cylinder, in which the auxiliary anode is placed, at the bottom end of a water body in water flow of the water discharging port, so that impact on the auxiliary anode and a cable by sea water can be effectively reduced and the fact that the auxiliary anode isthrust and washed away is avoided; in addition, a hollow structure of the side wall of the placing cylinder enables the auxiliary anode to be completely contacted with the sea water, the protective efficacy of a cathode protective system is enhanced and impact on the placing cylinder by the sea water can be reduced, so that the auxiliary anode can exert effect stably, has longer service life and reduces manual maintenance risk and cost.

The present invention adopts the monoclonal antibody 5D9 which has neutralizing activity to both PRRSV-I and PRRSV-II type viruses to make an immune enhancer, and when the formed immune complex is combined with an adjuvant to immunize mice, IFN-γ secretes T cells significantly Increased, suggesting that in the process of inactivated virus immunization, combined immunization with PRRSV-specific antibody 5D9 and normal oil-in-water adjuvant can enhance the CTL response. Animal experiments show that the immune protection rate of the immune complex prepared by the present invention can achieve higher protective efficacy than the vaccine prepared by only using the commercial adjuvant ISA 206 and the commercial attenuated vaccine.

The invention discloses a universal DNA vaccine of type A influenza virus and a construction method thereof, belonging to the technical field of genetic engineering. The DNA vaccine includes a gene construct, a carrier and a delivery system, the gene construct is inserted into the vector to construct an expression plasmid, and the delivery system is coated with the expression plasmid to form a universal DNA vaccine for influenza A virus, wherein the The gene construct is formed by serially combining the conserved sequence M2e genes of four different subtypes of influenza A viruses, and the delivery system is a cell-penetrating peptide. The vaccine combines the advantages of the extracellular region of influenza A virus M2 protein, universal vaccine and DNA vaccine, can effectively prevent and control different subtypes of influenza A virus, and has important scientific significance and social value.