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Nanocomposite of erythrocyte membrane coated functional molecules, preparation method and application

A nanocomposite and red blood cell membrane technology, applied in the field of preparation and application, can solve the problems of poor curative effect and short cycle time of targeted drugs, achieve low cost, facilitate large-scale production, and solve the effect of low efficiency

Pending Publication Date: 2021-11-16
CHINA PHARM UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Purpose of the invention: The present invention aims at the problems of short circulation time and poor curative effect of spleen or liver-targeted drugs in the prior art, and provides a nanocomposite of red blood cell membrane-coated functional molecules, and also provides the nanocomposite of the above nanocomposite Method and application

Method used

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  • Nanocomposite of erythrocyte membrane coated functional molecules, preparation method and application
  • Nanocomposite of erythrocyte membrane coated functional molecules, preparation method and application
  • Nanocomposite of erythrocyte membrane coated functional molecules, preparation method and application

Examples

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Effect test

Embodiment 1

[0032] Erythrocyte membrane-wrapped mRNA functional drug molecules target the spleen.

[0033] Extraction of erythrocyte membrane from Balb / c mice: This example takes the extraction of erythrocyte membrane from the blood of Balb / c mice as an example to illustrate, take 5mL of blood from the orbit, add 5ml of PBS (0.01mol / L, pH 7.4), and centrifuge at 4°C (3000rpm, 20min), carefully absorb the supernatant, intermediate white blood cells and platelet layer, blow the lower blood cell layer with phosphate buffered saline solution and mix well, then repeat the centrifugation and wash, take the packed red blood cells and dissolve them directly in 40 times the volume of pre-cooling in the permeabilization solution (hypotonic Tris-HCl 0.01M pH=8.0, PMSF 0.02mM), mix until the solution turns transparent and dark red, put it in the refrigerator at 4°C for 2h, centrifuge at 4°C, and discard the dark in the centrifuge tube. For the red supernatant, re-suspend the red flocculent precipitat...

Embodiment 2

[0036] Functionalized erythrocyte membrane-encapsulated mRNA drug molecules target the liver

[0037]Take the red blood cell membrane of Balb / c mice as an example. Take 5mL of blood from the orbit, add 5ml of PBS (0.01mol / L, pH 7.4), centrifuge at 4°C (3000rpm, 20min), carefully draw the supernatant, middle For the white blood cell and platelet layer, the lower blood cell layer was blown and mixed with phosphate buffered saline solution, and then centrifuged and washed repeatedly, and the packed red blood cells were directly dissolved in 40 times the volume of pre-cooled permeabilization solution (hypotonic Tris-HCl 0.01 M pH=8.0, PMSF 0.02mM), mix until the solution turns transparent and dark red, put it in a refrigerator at 4°C for 2 hours, then centrifuge at 4°C, discard the dark red supernatant in the centrifuge tube, and resuspend the red flocculent precipitate in Centrifuge at 4°C in the permeabilization solution, repeat washing three times until a milky white precipitat...

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Abstract

The invention discloses a preparation method and an application of a nanocomposite with functional molecules coated with an erythrocyte membrane. The nanocomposite comprises an erythrocyte membrane and functional molecules, wherein the functional molecules are mRNA, protein and small molecule drugs. According to the invention, the modified erythrocyte is prepared, the erythrocyte membrane coated nucleic acid drug is extracted, lipid components such as erythrocyte membrane phospholipid can make a functional drug carrier efficiently target the spleen, and the drug is delivered into the spleen or liver cells and released to play a therapeutic role; in addition, the cell membrane improves the in-vivo circulation stability and the intracellular delivery efficiency of the functional medicine. The nanocomposite is simple in preparation and extraction method, suitable for large-scale synthesis and preparation, mild in preparation condition, high in efficiency, uniform in particle size, good in dispersity, good in targeting capacity, high in biocompatibility and efficient and stable in intracellular delivery efficiency.

Description

technical field [0001] The invention relates to a nanocomposite of red blood cell membrane coated functional molecules, and also includes a preparation method and application of the nanocomposite. Background technique [0002] At present, cell membrane bionic technology has been widely used in drug delivery, tumor therapy, immune regulation and many other aspects. The use of cell membranes to wrap nanocomposites greatly improves biocompatibility, while achieving long-term circulation and targeted delivery in vivo. However, the half-life of the red blood cell membrane in the body is longer, and the residence time in the blood circulation is as long as 72 hours. The functional drug molecules wrapped by the red blood cell membrane have not only the physical and chemical properties of the nanocarrier itself, but also the biological properties of natural cells. It can capture pathogens on its own surface, neutralize them and present them to spleen immune cells, thus helping the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K47/69A61K47/54A61K45/00A61K31/7088A61K39/00A61P1/00A61P35/00A61P1/16B82Y5/00B82Y40/00
CPCA61K9/5176A61K9/5192A61K47/6901A61K47/545A61K47/6929A61K45/00A61K31/7088A61K39/00A61P1/00A61P35/00A61P1/16B82Y5/00B82Y40/00A61K2039/53
Inventor 乔海石叶优清郭宣诚
Owner CHINA PHARM UNIV
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