Method for preparing recombinant goose interferon I and II
A technology for interferon and purpose, applied in the field of preparation of goose recombinant I and II interferon
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[0061] The prokaryotic expression protein encoding the goose IFN-α mature protein (mIFN-α) and the IFN-γ mature protein (mIFN-γ) gene, the baculovirus / insect cell expression protein and the preparation method thereof include:
[0062] (1) Designing specific primers for amplifying the genes encoding goose IFN-α mature protein and encoding IFN-γ mature protein;
[0063] (2) Obtain the genes encoding goose IFN-α mature protein and IFN-γ mature protein by RT-PCR amplification;
[0064] (3) Cloning and sequencing identification of mIFN-α and mIFN-γ gene sequences respectively;
[0065] (4) Directional subcloning of mIFN-α and mIFN-γ genes into pET30a prokaryotic expression vector;
[0066] (5) Induced expression of mIFN-α and mIFN-γ genes in Escherichia coli respectively;
[0067] (6) Affinity chromatography purification of mIFN-α and mIFN-γ gene fusion expression protein, mainly including:
[0068] ① Affinity chromatography purification of mIFN-α gene fusion expression protein ...
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