The invention relates to a softening agent containing a sanguisorba officinalis l extract, wherein the components comprise, by mass, 20-31% of cyclocarya paliurus, 3-6% of a sanguisorba officinalis l extract, 5-8% of soybean isoflavone, 13-18% of natural essence, 2-3.5% of an epimedium extract, 10-18% of trichlorohydroxydiphenyl ether, 3-6% of Brazil essential oil, and 40-55% of deionized water, the cyclocarya paliurus is chloropropyltrimethoxysilane, the natural essence is imidazoline natural essence, and the Brazil essential oil is calcium chloride. According to the present invention, the softening agent has advantages of good softening and fluffiness effect after disinfection and washing, low use concentration, no damage on fabrics, good stability, no damage on skin, low cost and no environment pollution, can strongly kill common harmful microorganisms, especially pathogenic bacteria, and can ensure that the fabric does not contain bacterial and has characteristics of good static electricity resistance, good re-mixing wetting property, no damage and no yellowing after the washing.