The invention discloses a piece of antireflection glass. The antireflection glass is prepared by using aluminosilicate glass as a substrate; the visible light full-wave band average transmittance is higher than or equal to 94.5%, and the film coated surface single-side full-wave band average reflectivity is smaller than or equal to 1%; the antireflection glass is high in hardness, scrape resistance, acid-base resistance and follow-up processability. The antireflection glass comprises the aluminosilicate glass substrate, a first low-refractive index layer, a first high-refractive index layer, a second low-refractive index layer, a second high-refractive index layer, a third low-refractive index layer, a third high-refractive index layer, a fourth low-refractive index layer, a fourth high-refractive index layer, a fifth low-refractive index layer, and a fifth high-refractive index layer, wherein the first low-refractive index layer is made of SiO2; the first high-refractive index layer is made of Nb2O5. The antireflection glass is lower in the film coated surface single-side full-wave band average reflectivity, higher in hardness, and very suitable for acid and alkali treatment in the follow-up manufacturing procedures, so as to be adapted to more working procedures and achieve wider application range. The invention further discloses a preparation method of the antireflection glass.