The invention relates to an ELISA kit for detecting goose astrovirus antibody and a preparation method thereof, comprising an enzyme-labeled plate, an enzyme-labeled secondary antibody, a goose astrovirus positive control serum, a goose astrovirus negative control serum, a diluent, and a 10× Washing solution, TMB substrate solution, and stop solution; the antigen coated on the microtiter plate in the kit is the Cap protein of goose Astrovirus; the goose Astrovirus is avian Astrovirus type 3. The present invention expresses and purifies newly occurring Goose Astrovirus (Avian Astrovirus Type 3) Cap protein as the coating antigen, uses the indirect ELISA method to detect the goose serum sample to be tested, and assembles it for the determination of the Astrovirus antibody in the serum kit. The kit can be used for serological diagnosis of recent goose astrovirus infection, monitoring of antibody levels, epidemiological investigation, etc., and provides a simple and quick method for the prevention and control of goose astrovirus.