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Egg yolk antibody for preventing and treating novel goose astrovirus with cross-species transmission capacity and preparation method thereof

A technology of astrovirus and egg yolk antibody, which is applied in the direction of virus/bacteriophage, microbe-based methods, biochemical equipment and methods, etc., can solve the problems of inconvenient use and restrictions on the development and application of egg yolk antibody, and achieve good safety and good Prospects for commercial development, effectiveness in prevention and treatment of infections

Active Publication Date: 2021-02-09
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the disease is reported for the first time in my country, there is no commercial vaccine and antibody on the market, and the egg yolk antibody used for the emergency prevention and early infection treatment of the new goose astrovirus has not been reported; and because the egg yolk contains A large amount of lipids brings a lot of inconvenience to use, which also restricts the development and application of the yolk antibody for preventing and treating this novel goose astrovirus

Method used

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  • Egg yolk antibody for preventing and treating novel goose astrovirus with cross-species transmission capacity and preparation method thereof
  • Egg yolk antibody for preventing and treating novel goose astrovirus with cross-species transmission capacity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Isolation and Identification of Goose Astrovirus Strains

[0038] 1. Epidemiological investigation:

[0039] Since May 2019, an infectious disease characterized by nephritis and urate deposition in internal organs has broken out in ducklings in Shandong, Jiangsu and other places in my country. The disease mainly occurs in ducklings aged 3 to 20 days, and the mortality rate can reach up to 40%. The dead ducklings were autopsyed, and the main pathological changes were as follows: kidney enlargement and hemorrhage, degeneration, necrosis, and shedding of renal tubular epithelial cells.

[0040] 2. Collection and processing of disease materials:

[0041]Bacterial examination was carried out on the kidneys and other diseased organs of diseased ducks in a duck farm in Tai'an, Shandong. It was found that no bacteria were detected in the kidney tissue of the diseased duck, and the possibility of bacterial infection was preliminarily ruled out.

[0042] Then take ...

Embodiment 2

[0064] Embodiment 2: The characteristic investigation of new goose Astrovirus SDTA

[0065] 1. Pathogenicity of the virus:

[0066] 1.1 Pathogenicity to ducklings: 20 5-day-old healthy ducklings were randomly divided into two groups: the experimental group and the control group, and the experimental group was intramuscularly injected with the virus solution of the new goose astrovirus SDTA, 0.2ml / piece; The control group was injected with the same amount of sterile saline, and the clinical manifestations of ducklings in each group were observed for 14 days.

[0067] One duckling in the experimental group died on the 4th day, 6th day, 7th day and 9th day after the challenge, and the mortality rate reached 40% (4 / 10). Necropsy of dead ducklings showed renal enlargement and hemorrhage, degeneration, necrosis, and shedding of renal tubular epithelial cells.

[0068] The ducklings in the control group were all healthy and alive without any clinical manifestations. After 14 days o...

Embodiment 3

[0091] Embodiment 3: the preparation of inactivated vaccine

[0092] (1) Virus propagation and harvest:

[0093]Inoculate the isolated and identified novel goose astrovirus SDTA into well-grown DEK cells at an inoculation volume ratio of 1:100; adsorb at 37°C for 1 hour, and then culture in DMEM containing 2% newborn bovine serum at 37°C and 5% Cultivate under the condition of CO2, and harvest when 75% of the cells have lesions, and obtain the cell virus liquid. The obtained sufficient cell virus liquid was frozen at -20°C, and after two freezing and thawing, the supernatant was collected by centrifugation to obtain the virus liquid. Calculate TCID 50 , the virus content in every 0.1ml virus solution is 10 7.5 TCID 50 .

[0094] (2) Purification of virus:

[0095] Use established detection methods such as PCR and RT-PCR to detect whether other common viruses are contained in the obtained virus liquid. The detection items include avian influenza virus (AIV), Newcastle di...

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Abstract

The invention discloses an egg yolk antibody for preventing and treating a novel goose astrovirus with cross-species transmission capability. Inactivated novel goose astrovirus with a preservation number of CCTCC NO: V202019 is used as an antigen to prepare vaccine immune laying hens, and the egg yolk antibody is extracted and purified from high-immunity egg yolk. The goose astrovirus with the preservation number of CCTCC NO: V202019 is used as a vaccine production strain to prepare an inactivated vaccine to immunize laying hens, the egg yolk antibody can be efficiently obtained, the egg yolkantibody is good in safety, and any local or whole-body adverse reaction caused by the egg yolk antibody does not occur; and moreover, the egg yolk antibody can prevent and treat infection of the novel goose astrovirus effectively and has good commercial development prospects.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to an egg yolk antibody for preventing and treating a new type of goose astrovirus capable of cross-species transmission and a preparation method thereof. Background technique [0002] Astrovirus is a spherical non-enveloped single-stranded RNA virus with a diameter of 28-30 nm. In the ninth classification report of the International Committee for Isolation of Viruses (ICTV), the genetic distance of the capsid protein was used as the classification standard for virus species within the genus. , the avian Astrovirus genus is divided into three virus species names, namely Avastrovirus 1, Avastrovirus 2 and Avastwvirus 3. [0003] In May 2019, an infectious disease mainly characterized by nephritis and urate deposition in internal organs broke out in ducklings in Shandong, Jiangsu and other places in my country. The disease mainly occurs in ducklings aged 3 to 2...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/02C12N7/00A61K39/42A61P31/14C12R1/93
CPCC07K16/10C07K16/02C12N7/00A61P31/14C12N2770/12021C07K2317/35A61K2039/505A61K2039/552A61K2039/54
Inventor 刁有祥唐熠魏峰
Owner SHANDONG AGRICULTURAL UNIVERSITY
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