Goose astrovirus egg-yolk antibody compound and preparation method thereof

Abstract

The invention discloses a goose astrovirus egg-yolk antibody compound and a preparation method thereof and relates to the field of veterinary biopreparations. To achieve the objective, the preparation method comprises the steps of 1) using a goose astrovirus SD strain collected under CCTCC NO: V201815 as a vaccine producing viral strain to prepare an inactivation antigen; 2) subjecting the inactivation antigen to water phase preparation and oil phase preparation, mixing a water phase and an oil phase, and performing primary emulsification to obtain an immune antigen; 3) inoculating the immune antigen to layers so as to produce high-immunity eggs; and 4) collecting yolk from the high-immunity eggs, disinfecting, separating yolk, diluting the yolk, acidifying, extracting, preparing a stock antibody solution, filtering, concentrating, and inactivating to obtain the goose astrovirus refined egg-yolk antibody. Goose astrovirus can be prevented effectively, geese can be protected within the whole growth period, and farming benefit can be increased evidently.

Description

technical field [0001] The invention relates to a goose astrovirus egg yolk antibody complex and a preparation method thereof, and mainly relates to the field of veterinary biological products. Background technique [0002] Since 2013, goslings in the main goose breeding areas in my country have been prevalent with clinical symptoms of depression, decreased feed intake, emaciation, white lime-like loose stools, etc., and necropsy symptoms of kidney enlargement, mottled, ureter, muscle and There is urate deposition in the joints and urate adhesion on the surface of the heart and liver. It is clinically called goose "gout". The pathogenic agent of the disease has been identified as goose astrovirus. The disease mainly affects goslings less than 35 days old. The mortality rate of goslings less than 2 weeks old is within 40-70%, and that of 3-4 weeks old goslings is about 20%. The morbidity and mortality of goslings over 5 weeks old are relatively low. Conventional antiviral (tr...

AI Technical Summary

Problems solved by technology

[0006] The disadvantage of the goose astrovirus yolk antibody is that it needs repeated injections during the preventive protection period; the disadvantage of the goose trait virus inactivated vaccine is that there is an immune blank period, during which geese are susceptible to goose astrovirus and develop disease

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