Real-time fluorescence quantification PCR detection primer for goose astroviridae and kit of detection primer

Abstract

The invention provides a real-time fluorescence quantification PCR detection primer for goose astroviridae and a kit of the detection primer. The primer has the sequences represented by SEQ ID NO.1-2 and has very high specificity and sensitivity. Currently, a primer for a real-time fluorescence quantification PCR detection method for detecting the goose astroviridae based on saturated fluorescent dye Eva Green has not been researched and reported at home and abroad, and blanks in the relevant fields at home and abroad can be filled up by virtue of the detection primer and the kit.

Description

technical field [0001] The invention provides a real-time fluorescence quantitative PCR detection primer for goose astrovirus and a kit thereof, which belong to the field of animal infectious diseases. Background technique [0002] Astroviruses belong to the genus Astrovirus in the family Astroviridae and are divided into two genera according to different hosts: Mamastrovirus and Avastrovirus. There are currently three genera (Avastrovirus 1-3) in the genus Avian Astrovirus, including Duck astrovirus, Avian nephritis strovirus, Turkey astrovirus, etc. Symptovirus and its representative species. In recent years, with the continuous optimization of detection technology and the expansion of the detection field, the evolutionary distance between the new strain sequences obtained from humans and animals and the known astrovirus strain sequences is relatively long, and there may be further differences in the discovery of them. Multiple new subtypes and genotypes. Astrovirus is ...

AI Technical Summary

Problems solved by technology

At present, there is no relevant research report on the detection kit of Goose Astrovirus by Eva Green real-time fluorescence quantitative PCR at home and abroad. The establishment of the present invention can fill the gap in related fields at home and abroad.

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