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Novel goose astrovirus resulting gosling gout and application of novel goose astrovirus

A technology of astrovirus and goose gout, applied in the direction of viruses, antiviral agents, virus antigen components, etc., can solve the problems of economic losses in the goose industry, and achieve complete protection, good immunogenicity, and good safety effects

Active Publication Date: 2018-10-16
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease mainly occurs in goslings aged 5 to 20 days. Severe urate deposition occurs in the viscera and joint cavity of the diseased goose, and the mortality rate can reach up to 50%, which has caused huge economic losses to the goose industry.
[0004] For the epidemic of this infectious disease, there is currently no effective vaccine and drug for prevention and treatment. Therefore, starting from the pathogen, further research on the molecular pathogenic mechanism and virus mutation mechanism, and the development of corresponding vaccines are the key to the prevention and control of this infectious disease

Method used

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  • Novel goose astrovirus resulting gosling gout and application of novel goose astrovirus
  • Novel goose astrovirus resulting gosling gout and application of novel goose astrovirus
  • Novel goose astrovirus resulting gosling gout and application of novel goose astrovirus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Isolation and Identification of Goose Astrovirus Strains

[0024] 1. Materials and methods

[0025] 1.1 Collection and processing of disease materials:

[0026] Take liver, spleen, kidney and other tissues of dead goslings, add sterile saline at a ratio of 1:4 for homogenization, place the homogenate in the refrigerator for 3 times, centrifuge at 6000r / min for 15min, and take the supernatant The solution was filtered through a 0.22 μm filter and stored at -20°C for later use.

[0027] 1.2 Main reagents:

[0028] Goose embryos were purchased from a goose farm in Liaocheng, Shandong, the RNA extraction kit was purchased from Beijing Kangwei Century Co., Ltd., the ordinary agarose gel recovery kit was purchased from Beijing Quanshijin Company, and the reverse transcription kit was purchased from Bao Biology (Dalian ) Co., Ltd., 2×EsTaq MasterMix was purchased from Beijing Kangwei Century Co., Ltd., and DL2000Marker was purchased from Bao Biological (Dalian) C...

Embodiment 2

[0060] Embodiment 2: animal regression test

[0061] Sixty 1-day-old goslings without novel goose Astrovirus infection were equally divided into two groups, one was the control group and the other was the experimental group, with 30 goslings in each group. Goslings in the experimental group were subcutaneously inoculated with 0.2 mL of virus solution at the age of 5 days, and the control group was inoculated with 0.2 mL of sterile saline in the same way. The experimental group and the control group were kept in isolation, and the death of goslings was recorded every day, and the dead goslings were autopsyed observe.

[0062] Results: The goslings began to show depression and other symptoms on the first day after inoculation. On the 7th-10th day after inoculation, the death of goslings reached the peak period, and the mortality rate was 50%. After the goslings showed neurological symptoms such as opisthotonus, they died immediately. The necropsy of the dead goslings showed th...

Embodiment 3

[0063] Embodiment 3: the preparation of inactivated vaccine

[0064] (1) Virus propagation and harvest:

[0065] The isolated and identified novel goose astrovirus N-AStV-SDPY was inoculated into well-grown goose embryo kidney cells (GKC), and the virus was proliferated in large quantities to obtain enough virus liquid. The obtained sufficient amount of cell virus liquid was frozen at -20°C, and after two freezing and thawing, the cell virus liquid was collected.

[0066] (2) Purification of virus:

[0067] Use established detection methods such as PCR and RT-PCR to detect whether other common viruses are contained in the obtained virus liquid. The detection items include avian influenza virus (AIV), Newcastle disease virus (NDV), goose parvovirus (GPV), infectious bronchitis virus (IBV), infectious bursal virus (IBDV), etc., and the seed virus is purified .

[0068] (3) Inactivation of virus liquid:

[0069] The virus liquid that passed the bacterial inspection was inact...

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Abstract

The invention discloses a novel goose astrovirus which is preserved in the China Center for Type Culture Collection on April 26, 2018, and the preservation number is CCTCC NO: V201808. The novel gooseastrovirus disclosed by the invention has good immunogenicity upon popular goose astroviruses with a main symptom of gosling gout at present. An inactivated vaccine prepared from a novel goose astrovirus strain disclosed by the invention is good in security, has a protection rate up to 100%, and is capable of providing complete protection on newly separated goose astrovius variants.

Description

technical field [0001] The invention relates to the field of isolation and application of avian astrovirus strains, in particular to a novel goose astrovirus that causes goose gout and its application. Background technique [0002] Astrovirus (AStV) is a kind of non-encapsulated, spherical, single-stranded positive-sense RNA virus with a diameter of 28-30nm. The genome is infectious and 6.4-7.9kb long. The infection of this virus has a certain prevalence. It can cause enteritis and diarrhea in humans or animals, sometimes accompanied by vomiting and abdominal pain. Astroviridae can be divided into mammalian Astrovirus genus and avian Astrovirus genus according to the different infected hosts. Avian Astrovirus can cause a variety of diseases in poultry. vary with each other. [0003] From February to December 2017, an infectious disease mainly characterized by gout broke out in goose flocks in Shandong, Anhui, Jiangsu, Liaoning, Henan and Guangdong. The disease mainly occu...

Claims

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Application Information

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IPC IPC(8): C12N7/00C07K16/02A61K39/12A61P31/14C12R1/93
CPCA61K39/12A61K2039/5252A61K2039/552A61P31/14C07K16/02C12N7/00C12N2770/12021C12N2770/12034
Inventor 唐熠刁有祥田家军
Owner SHANDONG AGRICULTURAL UNIVERSITY
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