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Preparation and application of new goose astrovirus egg yolk antibody

A technology of egg yolk antibody and astrovirus, which is applied in the field of egg yolk antibody to achieve good commercial development prospects, effective immune protection and good safety effects

Active Publication Date: 2019-01-25
SHANDONG SINDER TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the fact that there is no commercial vaccine (or antibody) for the prevention and treatment of the disease at home and abroad, the disease is still spreading

Method used

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  • Preparation and application of new goose astrovirus egg yolk antibody
  • Preparation and application of new goose astrovirus egg yolk antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and Identification of GD 0122 Strain

[0030] 1. Epidemiological investigation Since October 2017, goslings in Guangdong, Sichuan, Hubei and other regions have had an infectious disease with high mortality and obvious symptoms of gout. The disease mainly occurs in goslings aged 5 to 15 days, and all breeds of geese are susceptible. Autopsies were performed on dead goslings. The main pathological changes are as follows: thickened pericardium, attached or coated with white crystals, commonly known as " Villi heart", the liver is covered with urate, the ureters become thicker, the metabolism is blocked, the kidneys are red and swollen or pale and enlarged, the kidneys are mottled, the contents of the end of the rectum are accumulated with white loose feces, the spleen is enlarged, and the intestinal wall is thinned, severe cases Visible joint swelling, urate deposition in the joint cavity. The inventor collected diseased geese from Guangdong, Sichuan a...

Embodiment 2

[0051] The preparation of embodiment 2 vaccine

[0052] Preparation of Virus Seeds of GD 0122 Strain

[0053] Dilute the GD 0122 virus strain 10 times, inoculate healthy goose embryos aged 12 to 13 days, 0.2ml / embryo, and culture them in an incubator with 60% humidity and 37°C for 168 hours, and discard goose embryos that died within 24 hours. The goose embryos that died within 168 hours were taken out immediately, and stored at 4°C. At 168 hours, all undead embryos were taken out, stored at 4°C for 12-24 hours, and the allantoic fluid of goose embryos was collected. According to this method, the goose embryo allantoic fluid harvested above was continued for 5 generations in 12-13 day-old goose embryos, which were respectively marked as D2-D6 generations. The virus content of each passage was determined. Will test for sterility and virus content ≥10 5.0 TCID 50 The virus liquid is mixed, quantitatively divided, and stored in a freeze-dried manner.

[0054] Preparation of ...

Embodiment 3

[0067] Example 3: Manufacture and inspection of refined egg yolk antibody against novel goose Astrovirus

[0068] 1 Production of high-free eggs

[0069] 1.1 Laying hens should have the production performance of commercial laying hens.

[0070] 1.1.1 No avian leukosis and avian influenza infection Take blood samples from 0.5% of the flock, all of which should be negative.

[0071] 1.1.2 Pullorum and Mycoplasma pullorum and Mycoplasma pullorum are detected according to NY / T536-2002 "Diagnostic Techniques for Typhoid and Pullorum" and NY / T553-2002 "Diagnostic Techniques for Mycoplasma Diseases", and the positive rate of pullorum and Mycoplasma infection should be ≤0.1 %.

[0072] 1.1.3 Feeding and management of laying hens The construction of chicken farms must meet the requirements of veterinary hygiene and epidemic prevention regulations. The chicken farm should be more than 500 meters away from the main traffic road, and the entrance and exit roads should be separated. Th...

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Abstract

The invention provides an egg yolk antibody prepared by using new goose astrovirus. The egg yolk antibody is prepared by taking inactivated vaccine prepared by using a new goose astrovirus GD 0122 strain with the preservation number of CCTCC No:V201820 as an antigen. The prepared antibody is good in safety, after a gosling is inoculated with the antibody, no local or systemic adverse reactions caused by the injection of the antibody occur; the prepared antibody is evaluated by adopting an antibody titer determination and challenge protection test, the result shows that the agar diffusion antibody titer of the prepared antibody is not lower than 1:16, after the antibody is injected, the gosling can resist the attack of virulent virus, and the prepared antibody can provide effective immune protection for geese and has a good commercial development prospect.

Description

technical field [0001] The invention belongs to the technical field of immune products, and in particular relates to an egg yolk antibody prepared by using a novel goose Astrovirus. technical background [0002] Gout, also known as urate deposition, nephrotic syndrome, is due to excessive production of uric acid in the body or obstruction of excretion, resulting in a large amount of uric acid accumulation in the body, and deposits in the form of urate in the joints, visceral organs, serosal surface and other interstitial tissues , thus causing slow movement, white loose feces, anorexia, exhaustion, and even death in affected poultry. It is a special type of protein metabolism disorder disease. [0003] Goose gout mainly occurs in young goslings, and it occurs frequently in 5-15 days old goslings. Sometimes it can be seen in 1-day-old goslings, and various breeds of geese can be affected, with a mortality rate of 30% to 70%. Sick geese 2 to 3 days before the onset Lime-like ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/02C07K16/10C07K1/34A61K39/42A61P15/08A61P19/06
CPCA61K2039/505A61P15/08A61P19/06C07K16/02C07K16/10
Inventor 常娓娓罗济冠王学波刘志亮王丹娜郭春丽王相芹康正武李朝阳
Owner SHANDONG SINDER TECH
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