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Goose astrovirus and application thereof

An astrovirus, n-goastv-190123 technology, applied in the direction of viruses, antiviral agents, virus antigen components, etc., can solve the problems of little research, no commercialization, and large economic losses of goose astrovirus vaccines

Active Publication Date: 2021-06-08
NANYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of suitable proliferative cell lines in vitro has resulted in relatively few attenuated or inactivated vaccines that can prevent and control avian Astrovirus infection, and there are no commercial goose Astrovirus vaccine products
[0004] The research and development of a variety of vaccines have been reported in the prior art. For example, Rong Xuelu et al. have developed and verified the immune effect of the recombinant Capsid protein subunit vaccine of goose Astrovirus. The research results show that the immune effect of the recombinant Capsid protein subunit vaccine of goose Astrovirus is Good, can effectively prevent the infection of goose astrovirus; patent document CN110699331A reports a preparation method and application of a goose astrovirus attenuated virus, which adopts a method of combining high temperature treatment and low temperature incubation to attenuate the virus for the preparation of vaccines It can effectively prevent goose gout caused by goose astrovirus. However, there are still few researches on goose astrovirus vaccines. Based on the current spread of goose astrovirus in farms, the economic losses caused are increasing. Therefore, There is an urgent need to develop safe and effective vaccines, yolk antibodies and drugs for the prevention and treatment of goose Astrovirus, in order to effectively control the Goose Astrovirus epidemic and avoid economic losses in the breeding industry

Method used

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  • Goose astrovirus and application thereof
  • Goose astrovirus and application thereof
  • Goose astrovirus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Isolation and Identification of Goose Astrovirus

[0023] 1. Collection of disease materials

[0024] The diseased material samples were collected from the affected farms in Nanyang, Henan Province. The affected goose species include: Taizhou goose and Lande goose. The dead goose showed joint swelling and white free urate deposition. Autopsy showed white urate deposition in the heart, Liver and kidney and other parts, some cases also have urate deposition in the subcutaneous and gallbladder.

[0025] Referring to the method in Zhang Qingshui's paper (Zhang Qingshui. Isolation and Identification of Emerging Nephropathogenic Goose Astroviruses and Breeding of Attenuated Virus Strains [D]. China Agricultural University, 2019.), the possible presence of the diseased goslings in samples Goose parvovirus (GPV), goose hemorrhagic polyomavirus (GHPV), duck reovirus (DRV), Tembusu virus (Tembusu) and goose astrovirus (GoAstV) and other viral genes were detected, amon...

Embodiment 2

[0043] Embodiment 2: the preparation of goose Astrovirus inactivated vaccine

[0044] 1. Preparation of Inactivated Vaccine

[0045] Step 1, take the goose astrovirus N-GoAstV-190123 virus seed of the 15th-20th generation and dilute it with a diluent, and dilute it to ELD 50 5×10 5.00 / mL, inoculate 8-10-day-old SPF goose embryos via the chorioallantoic membrane route, 0.2ml per embryo, and continue to incubate at 37°C after inoculation without turning the eggs;

[0046] Step 2: After inoculation of SPF goose embryos, light eggs 3 times a day, discard goose embryos that died before 24 hours, take out goose embryos that died after 24 hours at any time, and take out all live embryos at 72 hours, with the air chamber upward Stand upright and cool at 4°C for 12 hours;

[0047] Step 3: Take out the goose embryos that have been cooled for 12 hours. After the surface is sterilized, absorb the goose embryo fluid. The embryo fluid of every 20 goose embryos is mixed into one group. W...

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Abstract

The invention relates to a goose astrovirus N-GoAstV-190123 with strong virulence and good immune performance, after the goose astrovirus N-GoAstV-190123 is prepared into an inactivated vaccine for immunization, a relatively high neutralizing antibody can be generated, which can be used to protect a goose group completely, and has an important economic value for the goose breeding industry.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and mainly relates to the production of vaccines, in particular to a strain of goose astrovirus and its application. Background technique: [0002] Astrovirus is a non-enveloped, positive-strand RNA virus that can infect humans, dogs, cats, pigs, cattle, sheep, chickens, ducks and other animals. It is one of the main pathogens that cause diarrhea in animals, causing acute gastric Enteritis, lethal hepatitis in ducks, nephritis in chickens, visceral gout in geese and encephalitis in mammals. At present, astrovirus infection is widely prevalent in the world, and has become one of the important infectious diseases that threaten human health and economic animal breeding. In recent years, goose farms in Shandong, Jiangsu and Anhui have broken out a fatal infectious disease with gout as the main symptom. The disease mainly occurs in goslings aged 5-20 days. Infected goslings suffer from depression, loss...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/12A61P31/14C12R1/93
CPCC12N7/00A61K39/12A61P31/14C12N2770/12021C12N2770/12034A61K2039/5252A61K2039/552
Inventor 姚伦广冀君刘阳坤许鑫胡小敏
Owner NANYANG NORMAL UNIV
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