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Novel goose-star virus strain and application thereof

An astrovirus, a new type of technology, applied in the direction of viruses, antiviral agents, virus antigen components, etc., to achieve good commercial development prospects, effective immune protection, and good safety.

Active Publication Date: 2018-12-25
SHANDONG SINDER TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the fact that there is no commercial vaccine (or antibody) for the prevention and treatment of the disease at home and abroad, the disease is still spreading

Method used

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  • Novel goose-star virus strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Isolation and Identification of GD 0122 Strain

[0013] 1. Epidemiological investigation Since October 2017, goslings in Guangdong, Sichuan, Hubei and other regions have had an infectious disease with high mortality and obvious symptoms of gout. The disease mainly occurs in goslings aged 5 to 15 days, and all breeds of geese are susceptible. Autopsies were performed on dead goslings. The main pathological changes are as follows: thickened pericardium, attached or coated with white crystals, commonly known as " Villi heart", the liver is covered with urate, the ureter becomes thicker, the metabolism is blocked, the kidney is red and swollen or pale and enlarged, the kidney is mottled, the contents of the end of the rectum are accumulated with white loose feces, the gallbladder and spleen are enlarged, the intestinal wall is thinned, severe In some cases, it can be seen that the joints are red and swollen, and there are urate deposits in the joint cavity. Sever...

Embodiment 2

[0035] Preparation of Virus Seeds of GD 0122 Strain

[0036]Dilute the GD 0122 virus strain 10 times, inoculate healthy goose embryos aged 12 to 13 days, 0.2ml / embryo, and culture them in an incubator with 60% humidity and 37°C for 168 hours, and discard goose embryos that died within 24 hours. The goose embryos that died at ~168 hours were taken out immediately, and stored at 4°C. At 168 hours, all live embryos were taken out, stored at 4°C for 12-24 hours, and the allantoic fluid of goose embryos was collected. According to this method, the goose embryo allantoic fluid harvested above was continued for 5 generations in 12-13 day-old goose embryos, which were respectively marked as D2-D6 generations. The virus content of each passage was determined. Will test for sterility and virus content ≥10 5.0 TCID 50 The virus liquid is mixed, quantitatively divided, and stored in a freeze-dried manner.

Embodiment 3

[0038] Preparation of Antigen of GD 0122 Strain

[0039] 1. Take GD 0122 virus seed for inoculation, dilute it 10 times with sterilized PBS (0.1mol / L, pH value 7.2), inoculate healthy goose embryos aged 12 to 13 days, 0.2ml / embryo, seal the pinhole after inoculation, Place at 36-37°C to continue incubation without turning the embryos.

[0040] 2. Incubation and observation After the goose embryos were inoculated, the embryos were photographed twice a day, and the goose embryos that died 24 hours ago were discarded. Until 168 hours, regardless of death or not, all of them were taken out, the air chamber was upright, and cooled at 2-8°C for 12-24 hours.

[0041] 3. Harvesting Take out the cooled goose embryos, sterilize the air chamber with tincture of iodine, then aseptically peel off the egg shells in the air chamber, remove the egg membrane, cut the chorioallantoic membrane and amniotic membrane (to prevent the yolk from breaking), absorb Embryo fluid. Check each goose emb...

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Abstract

The invention provides a novel goose-star virus which can cause gosling gout and is provided with a GD 0122 strain. The strain is preserved in a Typical Chinese Culture Preservation Center of the Wuhan university on May 13, 2018, and the collection number of the strain is CCTCC No:V201820. The novel goose-star virus can be used for preparation of vaccines or antibodies for preventing gosling gout.The gosling gout vaccines prepared by the selected virus is good in safety, and any local and whole body adverse reaction caused by the vaccines are omitted. In a storage life test, character, safetytest and potency test data are analyzed, various indexes are stable and effective, immune effects of the vaccines are assessed by a serological method and an immune attacking method, results show that the prepared inactivated vaccine can provide effective immune protection for geese, and the virus has an excellent commercial development prospect.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a novel goose astrovirus strain and application thereof. technical background [0002] Gout, also known as urate deposition, nephrotic syndrome, is due to excessive production of uric acid in the body or obstruction of excretion, resulting in a large amount of uric acid accumulation in the body, and deposits in the form of urate in the joints, visceral organs, serosal surface and other interstitial tissues , thus causing slow movement, white loose feces, anorexia, exhaustion, and even death in affected poultry. It is a special type of protein metabolism disorder disease. [0003] Goose gout mainly occurs in young goslings, and it occurs frequently in 5-15 days old goslings. Sometimes it can be seen in 1-day-old goslings, and various breeds of geese can be affected, with a mortality rate of 30% to 70%. Sick geese 2 to 3 days before the onset Lim...

Claims

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Application Information

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IPC IPC(8): C12N7/00C07K16/10A61K39/12A61K39/395A61P31/14
CPCA61K39/12A61K2039/505A61K2039/5252A61K2039/552A61P31/14C07K16/10C12N7/00C12N2770/12021C12N2770/12034
Inventor 常娓娓罗济冠王学波刘志亮王丹娜郭春丽王相芹康正武李朝阳
Owner SHANDONG SINDER TECH
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