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Novel goose astrovirus with cross-species transmission capacity and application thereof

An astrovirus, a new type of technology, applied in the direction of medical preparations, viruses, antiviral agents containing active ingredients, etc., can solve the problems of vaccine-free and drug prevention and treatment, and achieve long-lasting immune protection and early harvest time. , good immunogenicity

Active Publication Date: 2021-01-29
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the fact that it is a new disease, there is still a lack of in-depth research on the epidemiology and pathogenic mechanism of the disease, and there is no effective vaccine and drug for prevention and treatment. Therefore, starting from the pathogen, further research on the molecular pathogenic mechanism and virus Mutation mechanism, the development of corresponding vaccines is the key to the prevention and control of this infectious disease

Method used

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  • Novel goose astrovirus with cross-species transmission capacity and application thereof
  • Novel goose astrovirus with cross-species transmission capacity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation and identification of goose astrovirus strains

[0029] 1. Epidemiological investigation:

[0030] Since May 2019, an infectious disease characterized by nephritis and urate deposition in internal organs has broken out in ducklings in Shandong, Jiangsu and other places in my country. The disease mainly occurs in 3 to 20-day-old ducklings, with a mortality rate of up to 40%. The necropsy of the dead ducklings showed that the main pathological changes were as follows: enlarged kidneys and hemorrhages, degeneration, necrosis and shedding of renal tubular epithelial cells.

[0031] 2. Collection and processing of sick materials:

[0032] Bacteriological tests were carried out on the kidneys and other diseased organs of diseased ducks in a duck farm in Tai'an, Shandong. The results showed that no bacteria were detected in the kidney tissue of the sick ducks, which preliminarily ruled out the possibility of bacterial infection.

[0033] Then the kidne...

Embodiment 2

[0055] Example 2: Characteristic investigation of novel goose astrovirus SDTA

[0056] 1. Pathogenicity of the virus:

[0057] 1.1 Pathogenicity to ducklings: 20 5-day-old healthy ducklings were randomly divided into two groups: the experimental group and the control group, the experimental group was intramuscularly injected with the virus solution of the new goose astrovirus SDTA, 0.2ml / duck; The control group was injected with the same amount of sterile normal saline, and the clinical manifestations of the ducklings in each group were observed for 14 days.

[0058] One of the ducklings in the experimental group died on the 4th, 6th, 7th and 9th days after the challenge, and the mortality rate was 40% (4 / 10). Necropsy of the dead ducklings revealed enlarged and hemorrhagic kidneys, degeneration, necrosis and shedding of renal tubular epithelial cells, and urate deposition on the surfaces of internal organs and joint cavities.

[0059] The ducklings in the control group were...

Embodiment 3

[0082] Example 3: Preparation of inactivated vaccine

[0083] (1) Virus proliferation and harvest:

[0084] The isolated and identified novel goose astrovirus SDTA was inoculated into DEK cells with a volume ratio of 1:100; adsorbed at 37°C for 1 h, and then cultured in DMEM containing 2% newborn bovine serum based on 37°C, 5% CO 2 Cultivated under the conditions of 75% of the cells, and harvested when 75% of the cells were diseased to obtain the cell virus liquid. The obtained sufficient amount of cell virus liquid was frozen at -20°C, and after two freeze-thaw cycles, the supernatant was collected by centrifugation to obtain a virus liquid. Calculate TCID 50 , the virus content in each 0.1ml virus liquid is 10 7.5 TCID 50 .

[0085] (2) Purification of virus:

[0086] Use established PCR, RT-PCR and other detection methods to detect whether the obtained virus liquid contains other common viruses. Test items include Avian Influenza Virus (AIV), Newcastle Disease Virus...

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Abstract

The invention discloses a novel goose astrovirus with cross-species transmission capacity and application thereof, the virus is preserved in China Center for Type Culture Collection on January 12, 2020, and the preservation number of the virus is CCTCC NO: V202019. The novel goose astrovirus provided by the invention has very good immunogenicity on currently popular goose astroviruses taking duckling nephritis as a main disease. An inactivated vaccine prepared from the novel goose astrovirus strain is good in safety, the protection rate reaches 100%, and complete protection can be provided fornewly separated goose astrovirus variants.

Description

technical field [0001] The invention relates to the field of isolation and application of avian Astrovirus strains, in particular to a novel goose Astrovirus with cross-species transmission capability and its application. Background technique [0002] Astroviruses (AStV) are a class of non-encapsulated, spherical, single-stranded positive-stranded RNA viruses with a diameter of 28-30 nm. The International Committee on Taxonomy of Viruses (ICTV) divides the Astroviridae family into two different genera according to the host of virus infection: Mamastrovirus and Avian Astrovirus Virus genus (Avastrovirus); Among them, avian Astrovirus can cause a variety of diseases in birds, and its clinical manifestations vary with virus strains, virulence or infecting hosts. [0003] In May 2019, an infectious disease characterized by nephritis and urate deposition in internal organs broke out in ducklings in Shandong, Jiangsu and other places in my country. The disease mainly occurs in 3...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/12A61P31/14A61P13/12C12R1/93
CPCC12N7/00A61K39/12A61P31/14A61P13/12C12N2770/12021C12N2770/12034C12N2770/12063A61K2039/5252A61K2039/552
Inventor 唐熠刁有祥魏峰
Owner SHANDONG AGRICULTURAL UNIVERSITY
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