Preparation method and product of goose astrovirus egg yolk antibody

An Astrovirus, Yolk Antibody Technology

Pending Publication Date: 2019-02-15
哈药集团生物疫苗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The existing methods for preparing goose astrovirus yolk antibodies have defects such as lo

Method used

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  • Preparation method and product of goose astrovirus egg yolk antibody
  • Preparation method and product of goose astrovirus egg yolk antibody
  • Preparation method and product of goose astrovirus egg yolk antibody

Examples

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Effect test

Embodiment 1

[0028] The preparation of embodiment 1 goose Astrovirus egg yolk antibody

[0029] 1. Test materials and preparation methods

[0030] The strain used for production: Goose Astrovirus HLJ strain used for making vaccine immunogen, which is isolated, identified, kept and sold by Harbin Pharmaceutical Group Biological Vaccine Co., Ltd.

[0031] 1 Preparation of virus stock solution

[0032] Use sterilized saline to make 10000-fold dilution of the virus seeds for production, inoculate 11-day-old susceptible duck embryos through the yolk sac, 0.1ml per embryo, seal the holes with wax, and incubate at 37°C. Duck embryos that died 48 hours ago were discarded, and eggs were illuminated every 6 hours thereafter, and duck embryos that died 48 to 120 hours were collected and cooled at 4°C for 6 to 12 hours. Duck embryos are disinfected with tincture of iodine on the surface of the air chamber of the eggshell, aseptically remove the eggshell on the air chamber, harvest the embryo fluid, ...

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Abstract

The invention discloses a preparation method and a product of a goose astrovirus egg yolk antibody. The preparation method comprises the following steps: (1) preparing an astrovirus liquid and an adjuvant, using the astrovirus liquid and the adjuvant as immunogens to immunize a healthy laying goose to prepare a highly immunized egg, collecting the highly immunized egg; (2) disinfecting the highlyimmunized egg, collecting an egg yolk; and extracting the egg yolk antibody from the egg yolk by using an acid water-carrageenan method. The recovery rate of the goose astrovirus egg yolk antibody extracted through the preparation method is more than 93%, the purity is more than 95%, and the indexes like the recovery rate, the extracting quantity and the purity of the goose astrovirus egg yolk antibody are obviously better than those of the goose astrovirus egg yolk antibody prepared by using the existing method. The goose astrovirus egg yolk antibody prepared by using the preparation method achieves the better protecting effect only by 0.5 ml of a preventive dose, and the rate of protection against the virulent strain reaches 100%. The infection and the outbreak of the goose astrovirus isprevented specifically, meanwhile, the production process is simplified, and the preparation method can implement large-scale production and is applied clinically.

Description

technical field [0001] The invention relates to a preparation method of the goose astrovirus yolk antibody, and further relates to the goose astrovirus yolk antibody obtained by the preparation method and its application, belonging to the field of preparation and application of the goose astrovirus yolk antibody. Background technique [0002] Gosling plague (Gosling plague), also known as goose parvovirus infection, is an acute septic infectious disease of goslings, characterized by lethargy, loss of appetite, severe diarrhea, sometimes neurological symptoms and high mortality. It can be infected through the digestive tract and respiratory tract, and can be transmitted vertically through eggs. The disease mainly affects susceptible goslings aged 4 to 20 days, and has the characteristics of rapid transmission, high morbidity and mortality. At present, the disease is distributed worldwide, often causing a large number of goslings to die, and has a great impact on the developm...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/02A61K39/12A61P31/14G01N33/569
CPCA61P31/14C07K16/02C07K16/10C07K2317/76C07K2317/11
Inventor 宋扬赵辉柴华卢爱国李应鹤李琳李贽武啸刘鑫莹曲海波
Owner 哈药集团生物疫苗有限公司
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