Preparation method and product of goose astrovirus egg yolk antibody

Abstract

The invention discloses a preparation method and a product of a goose astrovirus egg yolk antibody. The preparation method comprises the following steps: (1) preparing an astrovirus liquid and an adjuvant, using the astrovirus liquid and the adjuvant as immunogens to immunize a healthy laying goose to prepare a highly immunized egg, collecting the highly immunized egg; (2) disinfecting the highlyimmunized egg, collecting an egg yolk; and extracting the egg yolk antibody from the egg yolk by using an acid water-carrageenan method. The recovery rate of the goose astrovirus egg yolk antibody extracted through the preparation method is more than 93%, the purity is more than 95%, and the indexes like the recovery rate, the extracting quantity and the purity of the goose astrovirus egg yolk antibody are obviously better than those of the goose astrovirus egg yolk antibody prepared by using the existing method. The goose astrovirus egg yolk antibody prepared by using the preparation method achieves the better protecting effect only by 0.5 ml of a preventive dose, and the rate of protection against the virulent strain reaches 100%. The infection and the outbreak of the goose astrovirus isprevented specifically, meanwhile, the production process is simplified, and the preparation method can implement large-scale production and is applied clinically.

Description

technical field

[0001] The invention relates to a preparation method of the goose astrovirus yolk antibody, and further relates to the goose astrovirus yolk antibody obtained by the preparation method and its application, belonging to the field of preparation and application of the goose astrovirus yolk antibody. Background technique

[0002] Gosling plague (Gosling plague), also known as goose parvovirus infection, is an acute septic infectious disease of goslings, characterized by lethargy, loss of appetite, severe diarrhea, sometimes neurological symptoms and high mortality. It can be infected through the digestive tract and respiratory tract, and can be transmitted vertically through eggs. The disease mainly affects susceptible goslings aged 4 to 20 days, and has the characteristics of rapid transmission, high morbidity and mortality. At present, the disease is distributed worldwide, often causing a large number of goslings to die, and has a great impact on the developm...

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