16 results about "Yolk sac" patented technology

The present invention provides an RNA-based tumor vaccine, a preparation method, an immune adjuvant, a vector and an application. The tumor vaccine can specifically act on tumors with positive expression of GPC3, such as hepatocellular carcinoma, ovarian clear cell carcinoma, yolk sac carcinoma, etc. The tumor vaccine includes circular RNA capable of encoding an immunogenic polypeptide. The circular RNA is configured to be capable of specifically encoding the immunogenic polypeptide GPC3. The circ-GPC3 in the present invention has a covalently closed-loop structure that can protect it from exonuclease-mediated degradation, has a longer half-life and higher stability compared to mRNA, and can ensure the continuous expression of immunogenic proteins. By artificially modifying and introducing a ribosome entry site (IRES) modification, the present invention can increase the translation efficiency of tumor-specific antigen RNA.

The application belongs to the field of stem cell biology and relates to a culture method of a 3D nerve immune organoid containing microglia cells, which comprises the following steps: S1, inducing induced pluripotent stem cells in ectoderm direction and mesoderm direction respectively to obtain nerve-like embryoid bodies and yolk sac-like embryoid bodies; S2, culturing the nerve-like embryoid bodies to make them differentiate into nerves to obtain nerve ring structures, re-digesting the nerve ring structures to obtain nerve progenitor cell single cells, and culturing the yolk sac-like embryoid bodies to make them generate macrophage progenitor cells; and S3, fusing the nerve progenitor cell single cells and the macrophage progenitor cells and continuing to culture to form the 3D nerve immune organoid containing microglia cells. The brain-like organoid of the application can realize the following functions: exploring the control of microglia cells on the proportion of progenitor cells in the development stage, the change of phagocytosis, the control on the number of mature neurons, the influence on cell proliferation and apoptosis, and simultaneously accepting external stimulation and making corresponding functional stress changes.

The invention belongs to the technical field of biology, and particularly relates to an embryo in-vitro rotating culture method. The method comprises the following steps: culturing an embryo in an embryo culture solution, wherein the embryo culture solution contains fresh rat serum, antibiotics, ascorbic acid or a derivative thereof, pyruvic acid or a salt thereof, glutamine, a buffer salt and optional glucose; the embryo culture solution does not contain human umbilical cord blood serum; the use of human umbilical cord blood serum is avoided, so that the embryo rotating culture system can be quickly popularized; the method has the advantages that the method is simple and easy to operate, and further limits and adopts fresh rat serum and adjusts the oxygen concentration in different periods, so that the embryo proportion of blood continuously flowing in the egg sac blood vessel in the E7.5 + 3day period can be increased, the rapid flowing of the blood in the egg sac blood vessel in the E7.5 + 5day period of the mouse embryo is realized, and the in-vitro culture process of the mouse embryo E7.5-E7.5 + 6day is completed.

The present invention belongs to the field of biomedicine, and relates to an obstetric surgery risk prediction system, and specifically to a prediction system for massive bleeding during artificial abortion of unexpected pregnancy after cesarean section and a construction method thereof. The prediction system for massive bleeding during artificial abortion of unexpected pregnancy after cesarean section includes a variable input module and a nomogram model prediction module; the variable input module is used to input variables of the maximum diameter of the gestational sac, the number of cesarean sections, the medical history of uterine incision pregnancy, the distance between the gestational sac and the uterine incision scar, and the variable of the yolk sac visible by ultrasound; by substituting the values ​​of each variable into the nomogram model prediction module, the probability value of the risk of surgical bleeding can be obtained, which provides a reliable and effective reference for the configuration of obstetric surgery resources, and increases the investment in physical therapy resources for patients with higher bleeding risks. This scheme can solve the technical problem that the existing technology lacks a prediction system for the risk of massive bleeding during artificial abortion of unexpected pregnancy after cesarean section, and has an ideal prospect for application and promotion.

The present application belongs to the field of early nutrition of poultry in the livestock breeding industry, and relates to a method for increasing the hatching weight of chicks and promoting early growth and development and application, wherein taurine cholic acid solution is added to the air chamber of the chicken embryo; through observation of hatching time, incubation performance, growth performance, organ index, transport and absorption of nutrients in the yolk sac, and blood biochemical indicators, it is found that after the embryo egg is added with TCA, the hatching time of chicks is advanced, the incubation time is shortened, the embryo weight at E16 of chicks is significantly increased, the body weight of chicks after hatching is significantly increased, the growth of broilers during the feeding period after hatching is significantly promoted, and the leg muscle yield is significantly increased. In addition, the embryo egg added with TCA accelerates the transport of lipids in YSC to YSM by up-regulating the expression of E16 YSM and lipid transport related genes. The results show that the embryo egg added with taurine cholic acid can reliably promote the growth of broilers and laying hens during the incubation period and the growth of broilers after hatching.

The invention belongs to the technical field of health food with homology of medicine and food, particularly relates to a biological enzymolysis nano-type composition with a liver protection effect, a preparation method and application of the biological enzymolysis nano-type composition, and particularly relates to application of the biological enzymolysis nano-type composition in preparation of liver protection products, especially liver protection drugs. According to the present invention, the medicinal and edible components are treated through various means such as superfine grinding, double-enzyme enzymolysis combined with boiling water extraction, nanometer grinding and the like to obtain the biological enzymolysis nanometer type composition with the liver protection effect, and the experimental result shows that the biological enzymolysis nanometer type composition acts for 72 h under the concentration of 1.25 mg / mL, the yolk sac absorption promotion rate achieves more than 75%, and the liver protection effect is good. A good liver injury improving effect is shown.

The invention provides a culture medium and a culture method for embryo in-vitro culture, and particularly provides a culture medium which comprises a basic culture medium, serum and at least one substance related to metabolism. The culture medium can improve the development characteristics of mouse embryo in vitro culture (IVC) stages 1-3, including heart rate, body length, yolk sac diameter and survival rate.

The invention provides a constant-temperature circulating water artificial incubation and cultivation method for channel catfish, which comprises the following steps: introducing eyed eggs of channel catfish, building an indoor incubation device, and disinfecting the incubation device with potassium permanganate 7 days before incubation; the hatching device comprises a hatching tank and a hatching cylinder, the hatching tank is placed on the hatching cylinder, eyed eggs are uniformly spread at the bottom of the hatching tank, constant-temperature circulating water flow of 24.5-25.5 DEG C is filled in the hatching cylinder, and stirring and ventilation of a hatching water body are kept; the fries begin to float upwards to open for ingestion when membranes are broken and yolk sacs disappear, and the fries are successively transferred into a hatching tank in batches to be bred; the tenth day of transferring to the hatching tank is divided according to the size of the larvae, the larvae are divided once every 10 days, and the larvae are transferred to an indoor running water cement pond to be subjected to large-scale fingerling breeding until the average body length of each fish reaches 30 mm after 60 days. The method is suitable for breeding environments in Tibet regions, and has the advantages of high hatching rate and high survival rate.

Disclosed are methods of treating autoimmune diseases by administering engineered T cells expressing a chimeric antigen receptor (CAR) that specifically binds C-type lectin-like molecule-1 (CLL-1). The CAR-T cells deplete CLL-1–expressing monocyte-derived macrophages that replace yolk sac–derived or other embryonically derived tissue-resident macrophages, thereby reducing inflammation and disease progression. The methods are applicable to multiple sclerosis and other autoimmune disorders.

The invention provides an early pregnancy embryo ultrasonic maximum measurement section automatic grabbing system based on a real-time dynamic image, and the system comprises a multi-classification semantic segmentation module which is used for recognizing and segmenting a gestation sac, a yolk sac and a germ in an early pregnancy embryo ultrasonic dynamic image, and outputting a segmentation result; the measurement section capturing module is used for dynamically maintaining the longest diameter table of each category in the segmentation result based on the segmentation result, and determining the maximum measurement section of each category in the segmentation result according to a dynamic updating strategy; and the automatic measurement module is used for automatically measuring the maximum measurement tangent plane and generating a measurement result. The real-time performance and the stability of the system are improved, the measurement speed is remarkably improved, the consistency and the accuracy of diagnosis results are improved, and measurement errors caused by experience differences of doctors are reduced.

This invention relates to a dual-needle synergistic injection method for the yolk sac of zebrafish juveniles based on posture analysis, belonging to the field of zebrafish injection. The method comprises the following steps: Step 1) Arranging zebrafish regularly in a culture dish covered with agarose; Step 2) Ensuring the left and right needles are symmetrical; Step 3) Moving the stage to bring the zebrafish into the microscopic field of view, automatically determining the tail direction based on target detection results; Step 4) Moving the zebrafish yolk sac into the microscopic field of view and to the injection position; Step 5) Processing the target detection results and automatically determining the yolk sac direction; Step 6) If the yolk sac faces right, the injection is performed from right to left using the right needle; if the yolk sac faces left, the injection is performed from left to right using the left needle; Step 7) Moving the stage to the next zebrafish. This invention combines target detection and posture analysis, going beyond simply adjusting the zebrafish to a uniform posture to achieve more efficient and flexible automated batch injection.

The invention belongs to the field of stem cell biology, and relates to a culture method of a 3D neural immune organoid containing microglial cells, which comprises the following steps: S1, respectively inducing induced pluripotent stem cells in an ectodermal direction and a mesodermal direction to obtain a neural embryoid and a yolk sac embryoid; s2, culturing the neural embryoid to perform neural differentiation to obtain a neural garland structure, and re-digesting the neural garland structure to obtain a neural progenitor cell single cell; culturing the yolk sac embryoid to enable the yolk sac embryoid to generate macrophage progenitor cells; and S3, fusing the neural progenitor cell single cell and the macrophage progenitor cell, and continuously culturing to form the 3D neural immune organoid containing the microglial cells. The brain-like organ provided by the invention has the functions of exploring the control of microglial cells on the progenitor cell proportion, the change of phagocytic function, the control on the number of mature neurons and the influence on cell proliferation and apoptosis in the development stage, and meanwhile, the brain-like organ can accept external stimulation and make stress change of corresponding functions.

The application belongs to the technical field of biology and particularly relates to a method for in-vitro rotation culture of embryos. The method places the embryos in embryo culture solution for culture, wherein the embryo culture solution comprises fresh rat serum, antibiotics, ascorbic acid or derivatives thereof, pyruvic acid or salts thereof, glutamine, buffer salt and optionally glucose; the embryo culture solution does not contain human umbilical cord blood serum; the use of human umbilical cord blood serum is avoided, so that the embryo rotation culture system can be quickly popularized; and further limitation is made to the use of fresh rat serum, adjustment of oxygen concentration at different periods, which can improve the proportion of embryos with continued blood flow in the yolk sac blood vessels at the E7.5+3 day period, realize the rapid blood flow in the yolk sac blood vessels of mouse embryos at the E7.5+5 day period, and complete the in-vitro culture process of mouse embryos from E7.5 to E7.5+6 day.

Aiming at the characteristics of obese patients, the invention develops the siraitia grosvenorii-pericarpium citri reticulatae-black coffee solid beverage which is free of fat and contributes to reducing fat and burning fat. Medicinal and edible herbs such as fructus momordicae and orange peel (dried orange peel) are screened, dried orange peel filtrate is obtained through steam distillation-ultrasonic extraction, dried orange peel volatile oil is obtained through vacuum concentration, beta-cyclodextrin embedding and spray drying, and the original natural fragrance of the dried orange peel can be better reserved. On the basis, the dried orange peel extract obtained through spray drying is compounded with the momordica grosvenori extract to obtain momordica grosvenori orange peel (dried orange peel) composite powder, instant coffee powder and instant freeze-dried coffee powder are matched, and the best taste formula is obtained through sensory evaluation. Zebra fish experiments prove that the fructus momordicae and pericarpium citri reticulatae black coffee has the effect of reducing fat. The zebra fish has digestive systems similar to human beings, such as liver and intestinal tracts, and digestion and nutrient absorption and transportation are highly similar to human beings. About 70% of the yolk sac of the zebra fish is neutral fat, and if the absorption of the yolk sac of the zebra fish can be promoted, the decomposition and metabolism of fat can be promoted, which is similar to in-vivo fat combustion. After fat-specific fluorescent staining (red), the yolk sac of the zebra fish is dyed red, which can be obviously observed.

This method for producing hypoblast stem cells includes a step of culturing cells isolated from the yolk sac of a mammal in a culture medium containing an epidermal growth factor, a GSK3β inhibitor, and a ROCK inhibitor. This medium for inducing hypoblast stem cells contains an epidermal growth factor, a GSK3β inhibitor, and a ROCK inhibitor.

Disclosed are methods of treating autoimmune diseases by administering engineered T cells expressing a chimeric antigen receptor (CAR) that specifically binds C-type lectin-like molecule-1 (CLL-1). The CAR-T cells deplete CLL-1-expressing monocyte-derived macrophages that replace yolk sac-derived or other embryonically derived tissue-resident macrophages, thereby reducing inflammation and disease progression. The methods are applicable to multiple sclerosis and other autoimmune disorders.