2 results about "Yolk sac" patented technology

The application belongs to the field of stem cell biology and relates to a culture method of a 3D nerve immune organoid containing microglia cells, which comprises the following steps: S1, inducing induced pluripotent stem cells in ectoderm direction and mesoderm direction respectively to obtain nerve-like embryoid bodies and yolk sac-like embryoid bodies; S2, culturing the nerve-like embryoid bodies to make them differentiate into nerves to obtain nerve ring structures, re-digesting the nerve ring structures to obtain nerve progenitor cell single cells, and culturing the yolk sac-like embryoid bodies to make them generate macrophage progenitor cells; and S3, fusing the nerve progenitor cell single cells and the macrophage progenitor cells and continuing to culture to form the 3D nerve immune organoid containing microglia cells. The brain-like organoid of the application can realize the following functions: exploring the control of microglia cells on the proportion of progenitor cells in the development stage, the change of phagocytosis, the control on the number of mature neurons, the influence on cell proliferation and apoptosis, and simultaneously accepting external stimulation and making corresponding functional stress changes.

The application belongs to the technical field of biology and particularly relates to a method for in-vitro rotation culture of embryos. The method places the embryos in embryo culture solution for culture, wherein the embryo culture solution comprises fresh rat serum, antibiotics, ascorbic acid or derivatives thereof, pyruvic acid or salts thereof, glutamine, buffer salt and optionally glucose; the embryo culture solution does not contain human umbilical cord blood serum; the use of human umbilical cord blood serum is avoided, so that the embryo rotation culture system can be quickly popularized; and further limitation is made to the use of fresh rat serum, adjustment of oxygen concentration at different periods, which can improve the proportion of embryos with continued blood flow in the yolk sac blood vessels at the E7.5+3 day period, realize the rapid blood flow in the yolk sac blood vessels of mouse embryos at the E7.5+5 day period, and complete the in-vitro culture process of mouse embryos from E7.5 to E7.5+6 day.