56 results about "Yolk sac" patented technology

A handheld, hatchling holder not only simplifies manual examinations but also better protects the hatchling's welfare. The welfare concerns include not only bruising but also yolk-sac rupture. The handheld, hatchling holder has a cupped seat portion for cupping the ventral-end of a hatchling. The cupped seat portion is apertured, as with an opening. The hatchling is seated on the handheld, hatchling holder such that the cupped seat portion provides support to portions of the hatchling's perineal area that surrounds the alimentary vent (cloaca) as the alimentary vent is situated for exposure by the opening. The holder and opening facilitates not only welfare-friendly discharge of the meconium in the hatchling's alimentary vent through the opening but also welfare-friendly examination of the distended innards of the vent for markers of gender. The quality of the meconium discharge (or absence thereof) also facilitates determining whether the hatchling exceeds a hydration standard or not.

The invention belongs to the technical field of fish reproduction, and discloses a method for breeding Channaidae fish by using artificial insemination and hatching tanks. Selected broodstock are temporarily raised in a cement pond with a water temperature of 25-26°C, and induced labor is carried out; In a cement tank with a water temperature of 28-29°C, after the effect time is reached, check the egg squeeze every 2 hours, and perform a total of 3 times; after the egg squeeze, the broodstock is kept temporarily, and penicillin is used to prevent inflammation, and the female broodstock survives more than 75% ; The male fish takes the testis, and grinds it with a metal mesh in the sperm preservation solution; before fertilization, the sperm in the sperm preservation solution is activated with water, and then poured into the eggs for fertilization. The eggs are fertilized and left to stand for more than 2 hours, then hatched in the hatching bucket, and the hatching bucket is inflated; the fertilized eggs are hatched in the bucket for 2 days to produce membranes, and after another 1-2 days, the yolk sac is completely absorbed, and the seedlings are cultivated. The invention significantly improves the utilization rate of male broodstock and the postpartum survival rate of female broodstock, reduces site occupation and improves the success rate of seed hatching.

Fish embryos may be successfully vaccinated or therapeutically treated if the therapeutic agent is injected into the yolk sac. Therapeutic agents may be directly injected or released from microspheres and enter the circulation and tissues. Injection into the yolk sac, combined with the use of carriers, allows for a continued, controlled release of therapeutic agents and processing of antigens. Fish vaccination or therapeutic treatment, selecting fish embryos post fertilization at the one-cell to eyed egg stage of development, and injecting the yolk sac with carriers associated with an antigen(s) or therapeutic agent(s), may be fully automated.