56 results about "Yolk sac" patented technology

Positional cloning has been carried out to identify the gene responsible for the hypochromic anemia of the zebrafish mutant weissherbst. The gene, ferroportin1, encodes a novel multiple-transmembrane domain protein, expressed in the yolk sac. Zebrafish ferroportin1 is required for the transport of iron from maternally-derived yolk stores to the circulation, and functions as an iron exporter when expressed in Xenopus oocytes. Human and mouse homologs of the ferroportin1 gene have been identified. The invention includes isolated polynucleotides, vectors and host cells comprising nucleotide sequences encoding Ferroportin1 proteins and variants thereof, including those having iron transport function. The invention also includes polypeptides encoded by ferroportin1 genes and variants of such polypeptides, and fusion polypeptides comprising a Ferroportin1 or a portion thereof. Methods to produce a Ferroportin1, methods to produce antibodies to a Ferroportin1 and methods to identify agents binding to a Ferroportin1, which can be inhibitors or enhancers of Ferroportin1 iron transport activity, are also described. Inhibitors of Ferroportin1 activity can be used in a therapy for hemochromatosis.

The invention relates to the technical field of gears, in particular to a gear with an oil sac and a self-lubricating function. The gear comprises a gear body comprising a gear face. The outer periphery of the gear face is evenly provided with a plurality of outer gear teeth. The inner side of the outer periphery of the gear face is provided with a circular gear groove. The inner side of the circular gear groove of the gear face is provided with a circular cavity sac. The middle of each outer gear tooth on the outer periphery of the gear face is provided with a vertical channel communicated with the circular gear groove. The outer peripheral wall of the circular cavity sac is provided with a plurality of pores communicated with the circular gear groove. The gear is simple in structure; materials can be saved; lubricating oil is driven to seep to outer gear tooth grooves by rotation of the gear, the gear is lubricated accordingly, less maintenance is required, and normal orderly production is guaranteed.

The invention discloses a loach fry cultivation method. In April in spring, when a natural water body is higher than 18 DEG C in temperature, mature loaches which are regular in body form, strong in physique, much in mucus, healthy and scot-free are used as parents; LRH-A2 and DOM are used for hastening parturition; incubating is carried out at the water temperature which is 25 DEG C after oviposition, and loach fry cultivation is carried out after yolk sacs of most fries are basically disappeared after loach fry incubation; the loach fries are transferred to a pond to conduct cultivation according to a conventional summer fingerling cultivation technology after cultivation is carried out for seven days. According to the loach fry cultivation method, mixed feeding is carried out with yolk liquid and juvenile prawn mixed feed, the nutritional requirement of the mouth-opening periods of the loach fries is met, the loach fries rapidly grow, the complex process of rotifer cultivation is eliminated, the whole cultivation process is easy to operate, the labor amount is greatly reduced, meanwhile, the loach fry pond entering cultivation specification is increased, and the fry cultivation survival rate is improved.

A cross breeding method of odontobutis potamophila and odontobutis yaluensis is characterized by comprising the steps of parent selection and breeding, cross breeding and crossbreed fry rearing, wherein after fertilized egg membrane releasing is completed, a PVC pipe is timely taken out; after membrane releasing is carried out for 3 days, fry yolk sacs are eliminated; when fries can swim flatly, the fries start to open mouths to eat microplankton bait which mainly comprises wheel animalcule and cladocerans, and at least about 10 pieces of palatable bait are maintained underwater per milliliter. After membrane releasing is carried out for 10 days, the fries swim actively, certain copepoda, artemia and other kinds of macrozooplankton need to be supplemented at the moment, the feeding amount is properly added, and more than 15 pieces of the palatable bait are maintained under water per milliliter. When the fries are 1 centimeter long, the fries are shifted into a fry breeding pool where biological bait is cultivated in advance according to the density of 30-50 thousands fries per mu, small raw fishmeal or soybean cake powder is additionally thrown into the pool by means of additional points in the pool, and the daily feeding amount occupies about 5% of the weight of fish. The feeding frequency is twice a day in the morning and at afternoon. The average survival rate of juvenile fish bred by fry cross breeding reaches more than 85% and is higher than the survival rate of parent selfing.

The invention relates to a serum type 3 duck hepatitis A virus (DHAV-3) live vaccine and a preparation method thereof. The serum type 3 duck hepatitis A virus (DHAV-3) can only be separated and cultured by virtue of duck embryos generally at present, which creates a very big obstacle for the research of the virus and the research and manufacturing of a serum type 3 duck hepatitis live vaccine. According to the preparation method provided by the invention, a chick embryo yolk sac inoculation way is adopted, the serum type 3 duck hepatitis A virus is obtained by separating liver tissues of a duck with duck hepatitis, and an attenuated HuB60 strain (CGMCC No.10307) is obtained by virtue of chick embryo continuous passage culture, wherein the attenuated strain can be used for preparing the serum type 3 duck hepatitis A virus (DHAV-3) live vaccine, and can also be mixed with a serum type 1 duck hepatitis attenuated strain (such as A66 strain) according to an appropriate proportion to prepare a duck hepatitis A bivalent live vaccine.

The invention discloses a method for testing toxicity of environmental estrogen on whitebait embryonic development. The method of the invention utilizes the sensitivity of embryo to the simulation of environmental pollutant or chemical matter to perform a test under semi-static state on whitebait embryo which is exposed in standard diluted aqueous solution of subject having a series of concentrations. The test lasts about 30 days, begins as exposing a live embryo at the blastula stage in the aqueous solution of subject, and ends with hatching all embryos in a control group and an exposed group. The toxicity endpoint contains death, monstrosity and delayed hatching. The method comprises the steps of confirming the toxicity of the subject on the whitebait embryonic development by observing the toxicity endpoint of the exposed group and comparing with the control group; researching on the influence of two natural estrogens E2 and EE2 on the whitebait embryo-yolk sac stage so as to confirm the toxic dose-effect relationship of the natural estrogens to the whitebait embryonic development, and evaluating the potential risk of this type of fish exposed under the estrogen with environmental concentration.

The invention belongs to the technical field of detection. The invention discloses a method for evaluating antioxidant, spot-fading and skin-brightening efficacy by using a zebra fish model. The method comprises the following steps: carrying out an in-vitro experiment on zebra fish within 3 days after fertilization, taking menadione as a molding agent and an oxidative stress reagent as a dye, andtaking an experimental endpoint as the fourth day after fertilization; collecting a fluorescence picture and a white light picture of zebra fish yolk sacs as data, using yolk sac fluorescence intensity, namely software parameter brightness sum (B), for evaluating an antioxidant efficacy, and using yolk sac pigment intensity, namely software parameter opacity sum (O), for evaluating freckle-fadingand skin-brightening efficacy. The method uses menadione for the first time to establish evaluation of antioxidant, spot-fading and skin-brightening efficacy with the zebra fish model; establishes ananimal model that can evaluate antioxidant, spot-fading and skin-brightening efficacy of both oral health food and applying cosmetics and conforms to a principle of substitution; has a short experimental period, completes efficacy evaluation in 1-2 days, and can screen formulas in batches.

The invention discloses a method for lossless determination of genders of poultry embryo eggs. The method comprises the following steps of first, regularly hatching embryo eggs, second, acquiring embryo eggs with proper appearances, third, illuminating and detecting the embryo eggs according to a standard position, and fourth, determining a gender according to an embryo egg vitelline sac bloodline difference determination standard. The embryo egg vitelline sac bloodline difference determination standard is that when vitelline sac bloodline types on the left and right sides of the lower part of the embryo egg are the same, the gender is male; when the vitelline sac bloodline types on the left and right sides of the lower part of the embryo egg are different and the bloodline on the right-lower part is more developed than that of the left-lower part, the gender is female; and when vitelline sac bloodline types on the left and right sides of the lower part of the embryo egg are different and the bloodline on the left-lower part is more developed than that on the right-lower part, the gender is male. With the aid of the determination method for the gender of poultry embryo eggs, lossless determination for gender can be conducted for poultry embryo eggs in early stage; the embryo eggs can be selectively hatched as needed; embryo eggs non-required can be used for other purposes; and production efficiency can be further improved.

Provided is a high-yield culture method for marbled sand gobies, and belongs to the technical field of agriculture, forestry, animal husbandry, hunting, trapping, and fishing, and especially relates to a method for culturing fishes, mussels, crayfishes, lobsters, sponge, and pearls. The invention provides a high-yield culture method for marbled sand gobies, and the method makes fish body healthy and fast in weight gaining. The high-yield culture method for marbled sand gobies comprises the following steps: (1) preparing a pond, requirements of a pond used to culture marbled sand gobies being not strict, for providing convenience for daily management and overwintering covering, area preferably being 2.5 mu, sludge being little, and water resource being good; (2) putting fish fries, at present, fries of marbled sand gobies mostly being brought in from South East countries; (3) culturing fish fries, overall length of originally incubated fish fries being 2.3-2.6 mm, the whole body being transparent, and an abdomen having a big yolk sac; (4) performing culture managing and throwing bait, when the number of living little fishes and shrimps in the aquatic water is insufficient, the Thailand marbled sand gobies turning to eat artificial feed, feeding chilled small trash fishes or complete feed in artificial culture.

The invention belongs to the technical field of aquaculture, and discloses an in-vitro hatching method for cherax quadricarinatus embryos. The method comprises the steps that 1, ovigerous cherax quadricarinatus with the embryos in the mid-development stage and in orange yellow is obtained; 2, the embryos are slightly stripped along pleopods by using a comb, disinfected by using a disinfectant and washed with sterile water; 3, the washed embryos are hatched, the hatching water temperature is controlled to be 29-30 DEG C, water is replaced every 2-3 days, and dead embryos or dead shrimps are removed in time; 4, after being hatched, the embryos continue to be bred, when it is found that part of shrimp egg yolk sacs disappear, feeding is started, and feeding is carried out once every day until all the shrimp egg yolk sacs disappear to obtain shrimp seeds. The method greatly improves the egg carrying and hatching effects of parent cherax quadricarinatus, the hatching synchronism rate is relatively high, and a reference is provided for exploration and establishment of a breeding technology of the cherax quadricarinatus SPF seeds.

The invention discloses a method for breeding the silurus soldatovi from tiny fries to summer fries. The method breeds summer fries of fine quality by adopting technical measures including sterilizing the pond, controlling the water quality, putting in the fries, managing the breeding, etc. The calces is utilized to clean up the pond in the invention so as to increase the permeability of the silt at the bottom of the pond, increase the ionized calcium in the water, facilitate the reproduction of the aquatic organisms and effectively degerm and kill harmful bacteria as well as various aquatic animals. The silt at the bottom of the pond provides natural food for the fries put in the pond, which increases the growth and the survival rates of the fries. The shading net, immensed in the herbal medicine, is arranged on the surface of the pond, which facilitates the attachment of the fish body, prevents the wind wave, and reduces the diseases to facilitate the survival of the fries. When the fry yolk bags disappear to half, the tiny fries are put in the pond for stocking. The appropriate stocking density is 30,000-50,000 fries per mu, which provides sufficient natural food for the fries. The fries have a high rate of growth and a survival rate of 95%.

The invention discloses a method for detecting the toxicity of aflatoxin B1. The method comprises the following steps: preparing culture solutions with different aflatoxin B1 concentrations; culturing zebra fish embryos by virtue of the culture solutions with different aflatoxin B1 concentrations for 24-120 hours; and observing the death rate of the zebra fish embryos and the hatching rate, malformation rate, areas of heart cysts and yolk sacs and areas of fish larvae of young zebra fish hatched from the zebra fish embryos, calculating a heart cyst/larva area ratio and yolk sac/larva area ratio, and judging the toxicity effect of the aflatoxin B1 to the zebra fish embryos. According to the method, the aflatoxin B1 is detected by taking zebra fishes as an experiment object, so that a large number of experimental animals with good homogeneity can be rapidly obtained, the experiment cost is lowered, the toxic symptom can be easily observed, the pathological change of each tissue of the fish body can be visually observed in real time, and the detection efficiency, visuality and accuracy of the aflatoxin B1 are improved.

The invention belongs to the technical field of fish reproduction and discloses a method for reproducing snakehead fishes by using artificial insemination and a hatching barrel. The method comprises the following steps: temporarily rearing selected parent fishes in a cement pond at the water temperature of 25-26 DEG C and performing spawning induction; in the cement pond at the water temperature of 28-29 DEG C, after the effect time, carrying out inspection and ore squeezing once every 2 h, three times in total; temporarily rearing parent fishes after ore squeezing, preventing inflammation with a penicillin agent to keep the survival rate of female parent fishes at 75% or above; taking testes from male fishes, and grinding the testes in a sperm preservation solution in a separated manner with a metal net; and before fertilization, activating sperm in the sperm preservation solution, then pouring ores for fertilization, hatching the ores in the hatching barrel after leaving the ores tostand for more than 2 h, and inflating the hatching barrel; taking out fertilized ores after hatching the ores in the barrel for 2 days, and performing fry culture after yolk sacs are absorbed 1-2 days later. The method significantly the utilization rate of female parent fishes and the survival rate of the female parent fishes after spawning, reduces field occupation and increases the fry hatchingsuccess rate.

The invention discloses a method for artificial reproduction of pseudogy rincheilus procheilus. The method includes the steps that 1, mature and energetic pseudogy rincheilus procheilus is screened as parents; 2, the parents are cultured, wherein a, an outdoor cement pool is selected as a culture pool; b, gonad development of the parents is promoted through combination of hypophysis cerebri intramuscular injection and water level regulation stimulation, and culture is conducted; 3, artificial spawning induction is conducted, wherein after the water temperature in the middle ten days of April is reached, mature parents are screened in a hauling mode, and artificial spawning induction is conducted through a two-needle injection method; 4, artificial egg taking is conducted, wherein living body semen and egg collection is conducted; 5, artificial insemination is conducted, wherein artificial insemination is conducted on semen and egg individuals; 6, artificial incubation is conducted, wherein fertilized ova are washed with water for incubation, talcum powder or fresh milk is added for unsticking, after being completely washed, the fertilized ova are poured into Yushchenko incubation frames, dead ova are picked out after incubation, and after fry yolk sacs disappear and flatly swim, fry are moved out of incubation equipment and transferred into a fry culture cylinder to be cultured. The method is feasible and easy and convenient to operate, the fertilization rate and the incubation rate are high, the survival rate and the bait utilization rate are high, and the growth speed is higher than that of wild individuals.

A hand brake device is combined with a handlebar of a handle type vehicle and comprises a handlebar seat, an oil sac, a needle cylinder and a sac cover, wherein an accommodation chamber for accommodating the oil sac and a clamping part arranged on the rear side of the accommodation chamber are arranged on the handlebar seat; and the oil sac is combined with the sac cover to accommodate the needlecylinder in the body. The oil sac comprises an oil groove, a small beam ring and a large beam ring; the needle cylinder comprises a snap ring, a hollow oil chamber and an oil outlet; and the sac cover comprises a central through hole through which the needle cylinder penetrates, ring grains combined with the oil sac as well as an oil injecting port. By means of the shape, the structure and the combination of the shape and the structure, the handle type oil pressure hand brake device with the advantages of attractiveness in appearance, reduced wind resistance and capability of ensuring the smoothness of a brake oil channel is realized.

The invention discloses an image segmentation method based on feature extraction and denoising, and the method comprises the steps: firstly carrying out the segmentation to obtain a B ultrasonic image, and obtaining a pregnancy sac through the selection of an area where the pregnancy sac is located, feature extraction, denoising, convex hull detection and other algorithms; carrying out AND operation on a level set segmentation result graph and the gestational sac, and segmenting out the egg yolk sac-germ developmental state through the algorithms of feature extraction, morphology, denoising and the like; In the selected 149 pieces of data, the pregnancy sac segmentation accuracy rate is 94%, and the yolk sac-germ segmentation segmentation accuracy rate reaches 77.14%.

The invention discloses an artificial fancy carp hatching method, and belongs to the technical field of aquaculture. The artificial fancy carp hatching method comprises the following steps: performingdisinfection, aeration and dechlorination on a spawning pond before spawning; selecting parent fishes with strong physique and obvious strain characteristics to lay eggs and perform insemination; performing incubating on the fertilized eggs for 4-7 days to obtain fish fry; temporarily prohibiting feeding 24-36 hours after the fertilized eggs begin to form the fish fry; observing yolk sacs, and starting to feed filtered egg yolk and soybean milk when the yolk sacs are about to disappear and the fish fry begin to float upwards and swim horizontally; when swim bladders of the fish fry are inflated and yolk sacs completely disappear, adopting normal nutrient feed to feed the fish fry; after 7-10 days, putting the hatched fry into a soil pond for cultivation management, then classifying and screening the fry of the fancy carp, and reserving individuals with obvious characteristics and bright colors. The fancy carp fry cultured by using the fancy carp hatching method provided by the invention are high in survival rate, keep the characteristics of the original parent fish, and have great ornamental value.

The invention provides an oil seal underwater propeller and belongs to the field of underwater propellers. The oil seal underwater propeller comprises a propeller body and a compensator and further comprises an oil pipe, a head cover, a propeller oil pipe fixing sleeve, a shell, an oil seal gland, an oil seal outer retainer ring, an output shaft, a propelling screw, a sacrificial anode, an air deflector, an air deflector supporting rod, a skeleton-type oil seal, a speed reducer, a motor, a piston cylinder, a piston cylinder seat, a compensator oil pipe fixing sleeve, an oil pipe plug and an oil sac, the propeller body and the compensator are connected through the oil pipe, the head cover, the shell, the oil seal gland, the oil seal outer retainer ring and the output shaft are connected sequentially and matched with the skeleton-type oil seal to form a propeller sealing member, the air deflector is fixedly connected with the shell through the air defector supporting rod, and the propelling screw and the sacrificial anode are mounted the output shaft. The oil seal underwater propeller has the advantages that the problem of wearing of the propeller can be improved, reliability of a sealing structure can be improved, thickness of the shell of the propeller can be reduced, the problem of heat radiation of the motor can be solved, and loss of torque transmission can be avoided.

The invention discloses a chicken inclusion body hepatitis inactivated vaccine and a preparation method thereof. The virus is avian adenovirus group I serotype-4 virus named as SDJ15-8 with the preservation number of CCTCC NO: V201636 and is preserved in the China Center for Type Culture Collection in Wuhan University. The preparation method comprises the following steps: 1) inoculation: diluting the chicken inclusion body hepatitis virus with sterilized normal saline and then inoculating the diluted chicken inclusion body hepatitis virus into SPF chicken embryos through the yolk sac, standing and hatching at 37 DEG C, collecting chicken embryos after death within 48 to 120 hours, harvesting allantoic fluids and embryoid bodies, and crushing the embryoid bodies and then diluting with normal saline to obtain embryoid body fluids; 2) inactivation: uniformly mixing allantoic fluids embryoid body fluids which are qualified after tests according to a volume ratio of 2:1 to obtain mixed chicken embryo liquids, inactivating the mixed chicken embryo liquids with an inactivator and then detecting after inactivation; and 3) vaccine preparation: mixing the inactivated mixed chicken embryo liquids which are qualified after tests in step 2) with a transfer factor, and finally adding sterilized normal saline. The vaccine prepared from the virus and the transfer factor can effectively prevent and control diseases caused by the virus.

The invention relates to a method for building a model for reflecting influences of temperature and photoperiod on the utilization rate of pseudobagrus ussuriensis young fish yolk, and belongs to the technical field of research of pseudobagrus ussuriensis young fish yolk utilization. The method includes the following steps that pseudobagrus ussuriensis yolk sac larvae which are incubated and come out of membranes are obtained, and the pseudobagrus ussuriensis yolk sac larvae in the normal state are selected at room temperature to serve as test fishes; the test fishes are obtained and divided into multiple experiment factor combinations, and before an experiment, the average volume of yolk sacs of dozens of the larvae which are randomly selected serves as initial data; after testing is finished, 30 larvae are randomly selected in each experiment factor combination, and the sizes of egg diameters of the yolk sacs of the larvae in each experiment factor combination are measured; all the yolk utilization rate and the corresponding temperature and the photoperiod are obtained, the temperature and the photoperiod serve as independent variables, the yolk utilization rates serve as dependent variables, the least square method is used for fitting, and the model for reflecting the influences of the temperature and the photoperiod on the utilization rate of the pseudobagrus ussuriensis young fish yolk is obtained.

The invention discloses a chicken embryo cultivation method of Trionyx sinensis systemic sepsis spherical virus. The method is characterized in that an SPF chicken embryo is used as an in vitro culture system of Trionyx sinensis systemic sepsis spherical virus; a healthy chicken embryo which has been pre-cultured for 4 to 6 days is selected; a pre-prepared Trionyx sinensis systemic sepsis spherical virus inoculation fluid is innoculated to the chick embryo yolk sac; cultivation is continued until the death of the chick embryo; and the dead chick embryo is collected to carry out homogenate centrifugal separation and obtain a supernatant containing Trionyx sinensis systemic sepsis spherical virus. It is the first time to apply chick embryo culture technique into the cultivation of Trionyx sinensis systemic sepsis spherical virus. In addition, the method can accomplish fast and efficient in vitro subculturing of Trionyx sinensis systemic sepsis spherical virus and is stable and convenient.

The programmed inducing process of promoting directional differentiation of embryo stem cell to hemopoietic stem cell includes taking tissue, culturing stromal cell, collecting no-serum condition culture liquid, program inducing the directional differentiation of embryo stem cell to hemopoietic stem cell, sorting hemopoietic stem cell and other steps. The process features that embryo stem cell is cultured to form embryo proper and hemopoietic stromal cells in different growth stage are separated and cultured from yolk sac, embryo liver and adult myeloid tissue to form the cell layer in sub-fusion state. The present invention makes it possible to induce producing great amount of completely grown hemopoietic stem cells for clinical application.

The invention provides a method for culturing and preparing cordyceps militaris in an egg, and relates to the technical field of fungus culture. Solid and liquid culture mediums are optimized, a cordyceps militaris strain is bred, the cordyceps militaris strain is inoculated into the allantoic cavity and yolk sac cavity of a pollution-free egg according to a certain dosage to be cultured for a certain period of time under the proper temperature, humidity and dark environment, the mycelium growth optimum production conditions of the cordyceps militaris strain in the egg are studied according tothe inoculation dosage, part and time, the proper culture conditions and preparing method are determined, and the egg powder with cordyceps militaris is prepared and collected through the processes of disinfecting, smashing, pulping, filtering and then spray-drying. The technological method is simple, convenient to implement, controllable and stable and provides the technological basis and reference for industrial production of cordyceps militaris.