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Goose astrovirus bivalent inactivated vaccine, egg yolk antibody and preparation methods thereof

A bivalent inactivated vaccine and astrovirus technology, which is applied in the field of vaccine antibody preparation, can solve the problems of restricting the development of vaccines and related products, difficulties in cultivating goose astrovirus, cumbersome preparation of goose embryo kidney cells, etc., and achieve good commercialization Development prospects, no exogenous factor pollution, the effect of preventing goose gout

Active Publication Date: 2020-11-10
SHANDONG SINDER TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, goose embryos or goose embryo kidney cells are mostly used for culturing goose astroviruses, but goose embryos have the disadvantages of high cost, limited sources, low virus content, no SPF goose embryos, and easy contamination by exogenous factors; Kidney cells are cumbersome to prepare, small in quantity, and easily contaminated. Therefore, goose embryo or goose embryo kidney cells are not conducive to large-scale production
There are many difficulties in the cultivation of goose Astrovirus, which seriously restrict the development of vaccines and related products. At present, there are no commercially available bivalent inactivated vaccines and antibodies for effective prevention and treatment of the disease at home and abroad. Bivalent inactivated vaccines and antibodies against type and type 2 astroviruses are necessary requirements for the prevention and control of goose astroviruses

Method used

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  • Goose astrovirus bivalent inactivated vaccine, egg yolk antibody and preparation methods thereof
  • Goose astrovirus bivalent inactivated vaccine, egg yolk antibody and preparation methods thereof
  • Goose astrovirus bivalent inactivated vaccine, egg yolk antibody and preparation methods thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation method of the goose Astrovirus bivalent inactivated vaccine provided in this specific example comprises the steps:

[0040](1) Inoculate virus fluids of the novel goose astrovirus strain WF with the preservation number CCTCC No.V202047 and the novel goose astrovirus GD0122 strain (Noval goose astrovirus Strain GD 0122) with the preservation number CCTCC No.V201820 on 12 to 13 In day-old goose embryos, there is no serological relationship between the new goose astrovirus WF strain and the new goose astrovirus GD0122 strain, and the goose embryo allantoic fluid that died at 24 to 168 hours and the goose embryo allantois that survived at 168 hours were harvested Liquid, in this way continuous passage for 3 generations;

[0041] (2) Carry out virus identification on the virus liquid harvested after three generations of continuous passage, inoculate the virus liquid that meets the requirements in 12-13-day-old goose embryos, and harvest the goose embryo allan...

Embodiment 2

[0046] 1. Virus isolation and identification

[0047] In this specific example, two strains of goose astrovirus were used, namely the novel goose astrovirus WF strain and the novel goose astrovirus GD0122 strain (Noval goose astrovirus strain GD 0122). The novel goose astrovirus WF strain was released on July 2020 It was deposited in the Chinese Type Culture Collection Center of Wuhan University, Wuchang District, Wuhan City, Hubei Province on May 29, and the preservation number is: CCTCCNo. It is preserved in the Chinese Type Culture Collection Center of Wuhan University, Wuchang District, Wuhan City, Hubei Province, and the preservation number is: CCTCC No.V201820. The novel goose astrovirus WF strain is goose type 1 astrovirus, and the novel goose astrovirus GD0122 strain (Noval goose astrovirus strain GD 0122) is goose type 2 astrovirus.

[0048] The new goose astrovirus WF strain was isolated from a 6-day-old goose flock in a goose farm in Shandong, and the new goose ast...

Embodiment 3

[0113] This specific example provides a goose astrovirus bivalent yolk antibody, which is obtained from the goose astrovirus bivalent inactivated vaccine in Example 1 to immunize laying hens, and the specific steps for the preparation of the goose astrovirus bivalent yolk antibody for:

[0114] (a) immunize laying hens with the goose Astrovirus bivalent inactivated vaccine of embodiment 1:

[0115] Basic immunity: intramuscularly inject 0.5ml of goose Astrovirus bivalent inactivated vaccine into the chest of each chicken;

[0116] The second immunization: the second vaccination was carried out on the 14th day after the basic immunization, and 1.0ml of goose Astrovirus bivalent inactivated vaccine was injected intramuscularly into the chest of each chicken;

[0117] Three immunizations: the third inoculation was carried out on the 14th day after the second immunization, and 1.0ml of goose astrovirus bivalent inactivated vaccine was injected intramuscularly into the chest of ea...

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Abstract

The invention discloses a goose astrovirus bivalent inactivated vaccine, an egg yolk antibody and preparation methods thereof. According to the goose astrovirus bivalent inactivated vaccine, acclimatized LMH cells are inoculated with goose astrovirus type 1 strains and goose astrovirus type 2 strains separately for suspension culture, and a virus solution which is high in virus content and free ofconcentration is obtained; the goose astrovirus bivalent inactivated vaccine is obtained by mixing and emulsifying inactivated virus solution and a vaccine adjuvant; the goose astrovirus bivalent eggyolk antibody is obtained by immunize laying hens with the inactivated vaccine to obtain high-immunity eggs and then separating the egg yolk and extracting antibody. The goose astrovirus bivalent inactivated vaccine and the egg yolk antibody prepared by the invention can provide effective immune protection for goslings, can effectively prevent goose gout, and have a good commercial development prospect.

Description

technical field [0001] The invention belongs to the field of vaccine antibody preparation, and in particular relates to a goose astrovirus bivalent inactivated vaccine, egg yolk antibody and a preparation method thereof. Background technique [0002] Since 2016, infectious diseases mainly characterized by visceral and joint gout have broken out in goose farms in more than ten provinces including Henan, Jiangsu, and Shandong, causing serious economic losses to goose farms. The disease mainly affects goslings under the age of 3 weeks. Goslings at the age of 1 day can be seen as early as 1 day, and the mortality rate is 20% to 70%. White loose feces are discharged, and autopsy shows a large amount of urate deposition in the kidneys, heart, liver, lungs, ureters, and joints. The disease occurs in different breeds of geese that use different feeds and different drugs, and it is ineffective to reduce the protein content in the feed and reduce the amount of feed. [0003] Literat...

Claims

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Application Information

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IPC IPC(8): A61K39/12A61K39/295A61K39/42A61P31/14C12N7/00C12N7/02C07K16/10C07K16/02C07K1/34
CPCA61K39/12A61P31/14C12N7/00C07K16/10C07K16/02A61K2039/70A61K2039/5252C12N2770/12051C12N2770/12034C07K2317/11C07K2317/23
Inventor 徐丽娜罗济冠常娓娓王学波郭春丽刘宁李朝阳
Owner SHANDONG SINDER TECH
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